• Genomics & Informatics
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• v.6 no.4
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• pp.231-234
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• 2008

Precise and reliable identification of CNV is still important to fully understand the effect of CNV on genetic diversity and background of complex diseases. SNP marker has been used frequently to detect CNVs, but the analysis of SNP chip data for identifying CNV has not been well established. We compared various normalization methods for CNV analysis and suggest optimal normalization procedure for reliable CNV call. Four normal Koreans and NA10851 HapMap male samples were genotyped using Affymetrix Genome-Wide Human SNP array 5.0. We evaluated the effect of median and quantile normalization to find the optimal normalization for CNV detection based on SNP array data. We also explored the effect of Robust Multichip Average (RMA) background correction for each normalization process. In total, the following 4 combinations of normalization were tried: 1) Median normalization without RMA background correction, 2) Quantile normalization without RMA background correction, 3) Median normalization with RMA background correction, and 4) Quantile normalization with RMA background correction. CNV was called using SW-ARRAY algorithm. We applied 4 different combinations of normalization and compared the effect using intensity ratio profile, box plot, and MA plot. When we applied median and quantile normalizations without RMA background correction, both methods showed similar normalization effect and the final CNV calls were also similar in terms of number and size. In both median and quantile normalizations, RMA backgroundcorrection resulted in widening the range of intensity ratio distribution, which may suggest that RMA background correction may help to detect more CNVs compared to no correction.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.555-559
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• 2001

This research was conducted to estimate the chlorophyll contents of chrysanthemum leaves using SPAD-502 chlorophyll meter under different photoperiod and light intensity. Measurements were done at every third leaf intervals starting from the top of the stem to the bottom at harvest time. SPAD value was highest at 10 hours of photoperiods, followed by 13 and 16 hours of photoperiods. In particular, under short day condition, SPAD value was highest in the young leaves below the flower bud and decreased down the leaf profile. Under long day condition, SPAD value reached maximum at leaves between $15-27^{th}$ from the top and decreased to the minimum immediately below the flower bud (youngest leaf). These results may indicate that the younger leaves become strong source of supporting flowers in reproductive stage, while the younger leaves serve as sinks and older leaves support their development in vegetative stage. Changes of SPAD value at different levels of irradiance showed that highest SPAD value were observed at high irradiance and decreased with decreasing irradiance.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.17 no.6
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• pp.256-263
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• 2007

For $P_B=50Torr,\;P_T=5401Torr,\;T_S=450^{\circ}C,\;{\Delta}T=20K$, Ar=5, Pr=3.34, Le=0.01, Pe=4.16, Cv=1.05, adiabatic and linear thermal profiles at walls, the intensity of solutal convection (solutal Grashof number $Grs=7.86{\times}10^6$) is greater than that of thermal convection (thermal Grashof number $Grt=4.83{\times}10^5$) by one order of magnitude, which is based on the solutally buoyancy-driven convection due to the disparity in the molecular weights of the component A ($Hg_2Cl_2$) and B (He). With increasing the partial pressure of component B from 20 up to 800 Torr, the rate is decreased exponentially. It is also interesting that as the partial pressure of component B is increased by a factor of 2, the rate is approximately reduced by a half. For systems under consideration, the rate increases linearly and directly with the dimensionless Peclet number which reflects the intensity of condensation and sublimation at the crystal and source region. The convective transport decreases with lower g level and is changed to the diffusive mode at $0.1g_0$. In other words, for regions in which the g level is $0.1g_0$ or less, the diffusion-driven convection results in a parabolic velocity profile and a recirculating cell is not likely to occur. Therefore a gravitational acceleration level of less than $0.1g_0$ can be adequate to ensure purely diffusive transport.

• Journal of Biomedical Engineering Research
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• v.33 no.4
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• pp.169-176
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• 2012

Tumor cell morphology is closely related to its migratory behaviors. An active tumor cell has a highly irregular shape, whereas a spherical cell is inactive. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use 3D time-lapse phase-contrast microscopy to analyze single cell morphology because it enables to observe long-term activity of living cells without photobleaching and phototoxicity, which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we calculated the Fourier descriptor that morphological characteristics of cell to classify tumor cells into active and inactive groups. We validated classification accuracy by comparing our findings with manually obtained results.

• Food Science of Animal Resources
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• v.34 no.4
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• pp.552-557
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• 2014

This study investigated the effects of ${\gamma}$-aminobutylic acid (GABA) on the quality and sensorial properties of both the GABA/NaCl complex and model meat products. GABA/NaCl complex was prepared by spray-drying, and the surface dimensions, morphology, rheology, and saltiness were characterized. For model meat products, pork patties were prepared by replacing NaCl with GABA. For characteristics of the complex, increasing GABA concentration increased the surface dimensions of the complex. However, GABA did not affect the rheological properties of solutions containing the complex. The addition of 2% GABA exhibited significantly higher saltiness than the control (no GABA treatment). In the case of pork patties, sensory testing indicated that the addition of GABA decreased the saltiness intensity. Both the intensity of juiciness and tenderness of patties containing GABA also scored lower than the control, based on the NaCl reduction. These results were consistent with the quality characteristics (cooking loss and texture profile analysis). Nevertheless, overall acceptability of the pork patties showed that up to 1.5%, patties containing GABA did not significantly differ from the control. Consequently, the results indicated that GABA has a potential application in meat products, but also manifested a deterioration of quality by the NaCl reduction, which warrants further exploration.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.720-727
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• 2017

Objective: The aim of this work was to determine the relationship between objective sensory descriptors and volatile flavour compound composition of Polish traditional dry-cured loin. Methods: The volatile compounds were investigated by using solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). For sensory assessment, the quantitative descriptive analysis (QDA) method was used. Results: A total of 50 volatile compounds were found and assigned to 17 chemical families. Most of the detected volatile compounds derived from smoking, lipid oxidative reactions and seasoning (46.8%, 21.7%, and 18.9%, respectively). The dominant compounds were: aromatic hydrocarbon (toluene); alkanes (hexane, heptane, and 2,2,4-trimethylpentane); aldehyde (hexanal); alcohol (2-furanmethanol); ketone (3-hydroxy-2-butanone); phenol (guaiacol); and terpenes (eucalyptol, cymene, ${\gamma}-terpinen$, and limonene). Correlation analysis showed that some compounds derived from smoking were positively correlated with the intensity of cured meat odour and flavour and negatively with the intensity of dried meat odour and flavour, while terpenes were strongly correlated with odour and flavour of added spices. Conclusion: The analysed dry-cured loins were characterized by specific and unique sensory profile. Odour and flavour of studied loins was mainly determined by volatile compounds originating from smoking, seasoning and lipid oxidation. Obtained results suggest that smoking process is a crucial stage during Polish traditional dry-cured loins production.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.3
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• pp.281-288
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• 2004

The hybrid composite material (Al/GFRP laminates) are applied to the fuselage and wing in a aircraft. Therefore, Al/GFRP laminates suffer from the cyclic bending moments. This study was to evaluate the effect of fiber stacking angle on the fatigue crack propagation and delamination behavior using the relationship between crack growth rate (da/dN) and stress intensity factor range (ΔK) in Al/GFRP laminates under cyclic bending moment. The variable delamination growth behavior in case of three different type of fiber orientations, i.e., [Al/O$_2$/Al], [Al/+45$_2$/Al] and [Al/90$_2$/Al] at the interface of Al layer and glass fiber layer was measured by ultrasonic C-scan images. As results of this study, It represent that the delamination shape should turns out to have more effective characteristics on the fiber stacking angle. The extension of the delamination zone in case of [Al/+45$_2$/Al] and [Al/90$_2$/Al] were not formed along the fatigue crack profile. The shape of delamination zone depend on fiber stacking angle and the variable type with the delamination contour decreased non-linearly toward the crack tip at the Al layer.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.3
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• pp.394-401
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• 2002

The instantaneous flow characteristics of a round jet issuing normally into a crossflow has been studied using a flow visualization technique and particle image velocimetry. The effects of parameters such as jet inflow profile and turbulence intensity of the jet are evaluated for various Reynolds numbers in range between 735 and 3150, which are based on the crossflow velocity and jet-pipe diameter. The jet-to-crossflow velocity ratio is fixed at the value of 3.3. Instantaneous later tomographic images of the symmetry plane of the crossflow jet show that there exist very different natures in the flow structures of the near-field of the jet even though the velocity ratio is same. It is found that when the turbulence intensity of jet is elevated, the shear layer becomes much thicker due to the strong entrainment of the ambient fluid by turbulent interaction between the jet and crossflow. The detailed characteristics of instantaneous velocity and vorticity fields are presented to illustrate the effects of the above parameters on the vertical structures of the crossflow jet.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.51.1-51.1
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• 2013

We present molecular line observations of a small group of Young Stellar Objects (YSOs), L1251C. Observations by Spitzer Space Telescope legacy program "From Molecular Cores to Planet Forming Disks"(c2d; Evans et al. 2003) revealed that there are three YSOs within ~15" in L1251C: IRS1 (Class I), IRS2 (Class II), and IRS3 (Class II). In order to understand the molecular environment around these YSOs, we carried out the KVN single-dish observations in $HCO^+$ J=1-0, $H^{13}CO^+$ J=1-0, $N_2H^+$ J=1-0 and HCN J=1-0. CO J=1-0 was also mapped in L1251C with the TRAO 14m telescope. Integrated intensity maps of high density tracers such as $H^{13}CO^+$ J=1-0, $N_2H^+$ J=1-0 and HCN J=1-0 show similar emission distributions, whose peaks are off from the positions of YSOs. However, $HCO^+$ J=1-0, which is believed to trace both infall and outflow, presents its emission distribution different from those of other molecular transitions. The line profile of $HCO^+$ J=1-0 is superimposed by two velocity (narrow and broad) components. The $HCO^+$ outflow map reveals multiple structures while the CO outflow map elongates mainly along the EW direction. With the KVN single dish, the 22 GHz $H_2O$ maser emission has been also monitored toward L1251C to find variations of the systemic velocity and intensity with time.

• Journal of the Society of Naval Architects of Korea
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• v.60 no.2
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• pp.135-145
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• 2023

Developing high-fidelity Computational Fluid Dynamics (CFD) simulation methods used to evaluate the airwake characteristics along a flight deck of a large ship, the various kind of data such as actual ship measurement and wind tunnel results are required to verify the accuracy of CFD simulation. Inflow velocity profile at the bow, local unsteady flow field data around the flight deck, and highly reliable wind tunnel data which were measured after reviewing Atmospheric Boundary Layer (ABL) simulation and Reynolds Number effects were also used to determine the key parameters such as turbulence model, time resolution and accuracy, grid resolution and type, inflow condition, domain size, simulation length, and so on in STAR CCM⁺. Velocity ratio and turbulent intensity difference between Full-scale CFD and actual ship measurement at the measurement points show less than 2% and 1.7% respectively. And differences in velocity ratio and turbulence intensity between wind tunnel test and small-scale CFD are both less than 2.2%. Based upon this fact, the selected parameters in CFD simulation are highly reliable for a specific wind condition.