• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.821-825
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• 2018

A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The chromosome VII (1,105 kb) was successfully split to 887 kb, 44 kb containing the element for copy number amplification, and a 184-kb split-YAC. The 44-kb split-mini YAC was amplified a maximum of 9-fold, and the activity of the reporter enzymes integrated into the split-mini YAC increased about 5-7-fold. These results demonstrate that the mini-YAC containing a targeted chromosome region can be readily amplified, and the specific genes in the mini-YAC could be overexpressed by increasing the copy number.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.414-417
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• 2001

In today's engineering world, any product development activity requires the expertise and the interaction of many different engineering. Therefore the various activities in a product development architecture are highly distributed and interdependent. The interdependence may be due to sharing of databases and transfer of information between various activities. This will require an integrated product model. That can support distributed design such that participants from different backgrounds collaborate towards one common goal. This paper presents the architecture of the product modeling system for the integration of CAD and CAE. It then reports on the study of STEP 10303 and UG open API focused on the integration of different technical environments.

• Hwankyungkyoyuk
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• v.23 no.2
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• pp.16-31
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• 2010

The purpose of this study was to investigate the influence of the integrated atmosphere environmental education program on environmental attitudes for 5th graders in elementary school. The major results of this study were as follows. First, the developed atmosphere environmental education program had a positive effect on environmental attitudes of elementary students. Second, it also had a positive effect on their interest and desire of atmosphere environment issues. Third, it had a positive correlation among the 3 subdomains of cognitive, affective and behavioral. In conclusion, the atmosphere environmental education program is more directly effective than lectures on environment following the curriculum to improve students' attitudes toward environment. Therefore, it is required that more systematic research should be done linking with teaching and learning methods, as a follow-up activity.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1814-1820
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• 2023

Safety classification of systems, structures, and components (SSC) is an essential activity for nuclear reactor design and operation. The current regulatory trend is to require risk-informed safety classification that considers first, the severity, but also the frequency of SSC failures. While safety classification for nuclear power plants is covered in many regulatory and scientific publications, research reactors received less attention. Research reactors are typically of lower power but, at the same time, are less standardized i.e., have more variability in the design, operational modes, and operating conditions. This makes them more challenging when considering safety classification. This work presents the Integrated Risk-Informed Safety Classification (IRISC) procedure which is a novel extension of the IAEA recommended process with dedicated probabilistic treatment of research reactor designs. The article provides the details of probabilistic analysis performed within safety classification process to a degree that is often missing in most literature on the topic. The article presents insight from the implementation of the procedure in the safety classification for the MARIA Research Reactor operated by the National Center for Nuclear Research in Poland.

• Journal of Korean Society for Quality Management
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• v.32 no.3
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• pp.141-152
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• 2004

This study describes process and method to establish concurrent and/or sequential schedule planning for the system design process of the EIA632 standard. For this purpose, the study suggest Process and method that are context analysis of standard process, interface definition from activity decomposition, integration of related activities, and definition of concurrent and/or sequential work flow. The proposed process and method will contribute to minimize time loss that is emerged from activities iteration.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.70-80
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• 2016

This study was conducted to investigate the effects of an integrated recreation program on mood states, flexibility, and stress of elderly women. The integrated recreation program was applied to dance as a physical activity and songs and art as emotional activities in consideration of the characteristics of the elderly activities. The subjects were 50 elderly women aged 65 years or older who reside in K-do. The examination period was December 2 in 2014 to February 17 in 2015, during which time mood states, flexibilities, density of the salivary cortisol, variations in heartbeats, and stress were measured. The integrated recreation program had positive effects on mood, flexibility and stress reduction in elderly women. Based on these results, the integrated recreation program is considered to increase mood states and flexibility of elderly women while lowering stress. Therefore, the integrated recreation program developed in this study not only helps the physical and mental health of elderly women, but can also be widely utilized as a health promotion program for the elderly.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.339-344
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• 2009

Diabetic cataract is a major complication of diabetes mellitus. Excess accumulation of sorbitol plays an important role in the pathogenesis of diabetic complications such as cataract formation. In this study, we investigated the inhibitory effect of the extract of the aerial parts of Aster koraiensis (AK) on diabetic cataractogenesis. To examine this further, we evaluated sorbitol accumulation during cataract development using streptozotocin-induced diabetic rat, an animal model of type 1 diabetes. Diabetic rats were treated orally with AK (100 mg/kg and 200 mg/kg body weight) once a day orally for 9 weeks. In vehicle-treated diabetic rats, lens opacity was increased, and lens fiber swelling and membrane rupture were observed. In addition, sorbitol accumulation in diabetic lens was markedly enhanced. However, AK treatment delayed the progression of diabetic cataract through the inhibition of sorbitol accumulation, and prevented lens fiber degeneration in a dose-dependent manner. These observations suggest that AK treatment can delay the progression of lens opacification in the diabetic rats during the early diabetic cataractogenesis.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.980-985
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• 2009

The study was designed to test anti-platelet effect and find out anti-platelet mechanism of extract from Gamisopunghwalheol-tang in vitro. The extract was investigated for the inhibition against the aggregation of human platelet suspensions induced from collagen by aggregometer. And also the extract was investigated for the inhibition against the aggregation of human platelet suspensions who is taking aspirin or clopidogrel induced from collagen by aggregometer. In collagen-induced platelet aggregation test, the extract significantly inhibited collagen-induced platelet aggregation in a concentration-dependent manner(p<0.05). The extract significantly inhibited collagen-induced platelet aggregation of human platelet who is taking aspirin or clopidogrel ing 0 mg/ml concentration(p<0.05). And the extract inhibited more ingpatients who is taking aspirin. These results show that the extract from Gamisopunghwalheol-tang has anti-platelet aggregation effect.

• Journal of Families and Better Life
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• v.25 no.4
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• pp.57-66
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• 2007

This study purposed to examine the effects of integrated activities based on wordless picture books on increase in children's creativity. As for the subject of this study, 20 five-year old children(9 boys and 11 girls), who go to D Kindergarten in Ulsan-si, were selected as a experimental group and 20 five-year old children(8 boys and 12 girls) as comparative group, who attend B Kindergarten in Ulsan-si. For this study, all children of the groups were taken pre-test. After this experiment, difference in creativity of two groups was examined through post-test. The total of 42 sessions were conducted for 14 weeks from the first week of April to the second week of July, 2005. The test tool for this study was Creativity Scale developed by Kim Sang-yun (2003). To verify study questions, the mean of the two groups and the standard deviation were calculated and independent-sample t-test was carried out using SPSS WIN 11.5. The results of this study were as the following: First, integrated activities based on wordless picture books showed statistically significant difference in children's creativity between the experimental group and the comparative one(p<.001), which suggested that the activities affected increase in children's creativity in general. Second, integrated activities centering around wordless picture books indicated statistically significant difference in fluency(p<.05), flexibility(p<.01) and originality(p<.001), sub-factors of children's creativity, between the experimental group and the comparative one. This result revealed that these activities were effective. Third, integrated activities based on wordless picture books did not show significant difference in elaboration, a sub-factor of children's creativity, between the two groups. As the result of this study, integrated activities based on wordless picture books had positive effects on increase in children's creativity.

• Clinical and Experimental Reproductive Medicine
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• v.51 no.2
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• pp.102-111
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• 2024

Objective: Given the noteworthy implications of alcohol consumption and its association with male infertility, there has been a notable focus on investigating natural alternatives to mitigate its adverse effects. Thus, this study was conducted to assess the potential protective effect of phycocyanin extract derived from the blue algae Arthrospira (Spirulina) platensis against ethanol-induced oxidative stress, disturbances in testicular morphology, and alterations in sperm production. Methods: Male rats were divided into four groups (five rats each): the control group received a saline solution, the ethanol exposed group (EtOH) was subjected to intraperitoneal injections of 10 mL/kg of ethanol solution at a concentration of 38% (v/v), the phycocyanin alone treated group (P) received oral administration of phycocyanin at a dosage of 50 mg/kg, and the phycocyanin-cotreated group (PE) was given oral phycocyanin followed by ethanol injections. All treatments were administered over a period of 14 days. Results: Our findings demonstrated that ethanol exposure induced reproductive toxicity, characterized by reduced sperm production and viability, alterations in testicular weight and morphology, increased lipid peroxidation levels, and elevated oxidative enzyme activity. In addition, the ethanol-intoxicated group showed perturbations in serum biochemical parameters. However, the simultaneous exposure to ethanol and phycocyanin exhibited a counteractive effect against ethanol toxicity. Conclusion: The results showed that supplementation of phycocyanin prevented oxidative and testicular morphological damage-induced by ethanol and maintained normal sperm production, and viability.