• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.593-598
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• 1997

The changes of tissue structure in timothy and alfalfa during ensiling process with silage additives; lactic acid bacteria, cellulase and formic acid, were observed with a video microscope. Stem samples were obtained from the second internode, and cut to divide into 2 pieces. One piece was for observation of ensiled material and the other was for silage. The latter piece was put into a nylon cloth bag, and ensiled with grass for 50 days in a small experimental silo Lignification of the plant tissues was checked by acid phloroglucinol. Natural silage fermentation resulted in some degradation of less lignified parenchyma in both plant species. However, lignified sclerenchyma and vascular bundles remained intact. The cellulase enhanced the degradation of parenchyma tissue, while the formic acid suppressed the degradation. The effect of lactobacillus was small. The percentage of remained cross sectional area of stem and the loss of NDF and ADF by silage fermentation confirmed the observation. High negative correlations were obtained between the remained area and loss of fibrous components during silage fermentation in both plants, and between the loss of fibrous components and in vitro dry matter digestibility in timothy but not in alfalfa.

• The korean journal of orthodontics
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• v.1 no.1
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• pp.15-20
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• 1970

The author investigated on the responses of pulp and dentine following tooth movements. The material consisted of fifty-four intact teeth from twenty-seven adult white rats. The half of the teeth were employed as controls and the other half served as experimental group. These teeth were moved with forces ranging from 30 grams to 120 grams for from 3 to 7 days. All these were extracted immediately after the force was relieved. The main pulp changes in the experiment were vacuolization of the pulp tissue and circulatory disturbances. The magnitude of the force had an important role. In addition to these changes, the resorption in dentine and cementum was observed, which was related to the magnitude of the force and the duration of experiment.

• Biomedical Science Letters
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• v.9 no.3
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• pp.111-121
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• 2003

Recombinant single chain Fv (scFv) antibodies offer many advantages over mouse monoclonal antibodies such as faster clearance from blood, improved tumor localization, reduced human anti-mouse antibody (HAMA) response, and the availability to manipulate the scFv through genetic approaches. The recombinant phage display was constructed using lym-l hybridoma cells as a source of genetic starting material. mRNA was isolated from the corresponding antibodies hybridoma cells. VH and VL cDNA were amplified with RT-PCR and linked with ScFv by linker DNA to form ScFv DNA, which then were inserted into phagemid pCANTAB5E. The phage of positive clones selected with tube containing raji lymphoma cell and infected by competent E. coli HB2151 to express soluble scFv. The scFv lym-l was secreted into the cytosol and culture supernatant and shown to be of expected size (approximately 32 kDa) by western blot. An active scFv lym-l could be produced in E. coli with soluble form and high yield from hybridoma cell line, using phage display system. Immunoreactivity indicated that scFv lym1 showed a potential biding affinity against the raji lymphoma cell as its parental antibody (intact lym-l Ab).

• Journal of Plant Biology
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• v.16 no.1_2
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• pp.17-22
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• 1973

The experiments were conducted to examine the injuries of $SO_2$, $NH_3$, and $SO_2$ and NH3 mixed gas to the germination and the growth of plants. Six kinds of plants were used as the material. These plants seeds were treated with the gases for five days. Rate of the germination and the growth in height of varying plants were different according to the components of the gases. The critical concentration of the gases for both the germination and the growth were in 5ppm of $SO_2$, 50ppm of $NH_3$, and 50ppm $SO_2$ and 50ppm $NH_3$ mixed gases. When a low concentration of $SO_2$ was treated together with NH3, especially it was reduced to 60 percent of the damage in the germination and the growth. In the treatemnt with $SO_2$, the germination of the seeds which soaked in water for 24 hours reduced the injuries more 40 percent than those which for one hour. It was observed that the seeds with thick coasts or with originally intact coats were suffered but little damaged by the gases, and the external symptoms of an injury were shown, at first, in water pore, and then, in guard cell on the leaves.

• Elastomers and Composites
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• v.55 no.2
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• pp.114-119
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• 2020

The effect of a greenhouse-covering material on its indoor environment and on the characteristics of cherry tomatoes grown in it was investigated. The conventional polyethylene (PE) film on the greenhouse roof was replaced by a polycarbonate (PC) sheet, while maintaining the main structural frame intact. Color changes and the formation of water droplets on the PC surface were avoided by applying coextrusion and coating layers. When compared to the PE greenhouse, the PC greenhouse enabled increased light transmittance and thus a higher indoor temperature during both summer and winter. The thermal insulating property of the PC sheet effectively reduced the heating loss by approximately 55% during winter. The cherry tomatoes grown in the PC greenhouse exhibited superior fruit characteristics in terms of size, weight, and sugar content. The total amount of cherry tomatoes produced per unit area (1,000 ㎡) in the PC greenhouse was found to be greater by approximately 19% compared to that in the PE greenhouse.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.394-399
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• 2005

In this paper, a structural damage identification method (SDIM) is developed to identify the line crack-like directional damages generated within a cylindrical shell. First, the equations of motion fur a damaged cylindrical shell are derived. Based on a theory of continuum damage mechanics, a small material volume containing a directional damage is represented by the effective orthotropic elastic stiffness, which is dependent of the size and the orientation of the damage with respect to the global coordinates. The present SDIM is then derived from the frequency response function (FRF) directly solved from the dynamic equations of the damaged cylindrical shell. In contrast with most existing SDIMs which require the modal parameters measured in both intact and damaged states, the present SDIM requires only the FRF-data measured in damaged state. By virtue of utilizing FRF-data, one may choose as many sets of excitation frequency and FRF measurement point as needed to acquire a sufficient number of equations fer damage identification analysis. The numerically simulated damage identification tests are conducted to study the feasibility of the present SDIM.

• Steel and Composite Structures
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• v.35 no.2
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• pp.171-186
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• 2020

This paper aims at providing insights on the use of thermosetting liner for the repair of offshore pipelines exposed to corrosion and leakage. The work which covers both experimental and numerical approaches were aspired due to the high cost of repair for pipelines, limitations of thermoplastic material and limited study of reinforced thermosetting liner. The experiment involves a destruction test called the burst test, carried out on an API 5L X42 carbon steel pipe under four case studies, namely (i) intact pipe, (ii) pipe with corrosion defect, (iii) pipe with corrosion defect and repaired with thermosetting liner and (iv) pipe with leakage and repaired with thermosetting liner. The numerical simulation was developed to first validate the experimental results and later to optimize the design of the thermosetting liner in terms of the number of layers required to restore the original strength of the pipe. The burst test shows an improvement in 23% of the burst capacity for the pipe with corrosion defects, after being repaired with a three-layer thermosetting liner. The parametric studies conducted showed that with an addition of thermosetting layers, the burst capacity improves by an average of 1.85 MPa. In conclusions, the improvement in strength can be further increased with increasing thickness of the thermosetting liner. The thermosetting liner was also determined to fail first inside the host pipe.

• Earthquakes and Structures
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• v.19 no.6
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• pp.445-457
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• 2020

An ultra-high voltage (UHV) transmission system has the advantages of low circuitry loss, high bulk capacity and long-distance transmission capabilities over conventional transmission systems, but it is easier for this system to cross fault rupture zones and become damaged during earthquakes. This paper experimentally and numerically investigates the seismic responses and collapse failure of a UHV transmission tower-line system crossing a fault. A 1:25 reduced-scale model is constructed and tested by using shaking tables to evaluate the influence of the forward-directivity and fling-step effects on the responses of suspension-type towers. Furthermore, the collapse failure tests of the system under specific cross-fault scenarios are carried out. The corresponding finite element (FE) model is established in ABAQUS software and verified based on the Tian-Ma-Qu material model. The results reveal that the seismic responses of the transmission system under the cross-fault scenario are larger than those under the near-fault scenario, and the permanent ground displacements in the fling-step ground motions tend to magnify the seismic responses of the fault-crossing transmission system. The critical collapse peak ground acceleration (PGA), failure mode and weak position determined by the model experiment and numerical simulation are in relatively good agreement. The sequential failure of the members in Segments 4 and 5 leads to the collapse of the entire model, whereas other segments basically remain in the intact state.

• Steel and Composite Structures
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• v.39 no.5
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• pp.543-564
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• 2021

In this study free vibration analysis of a cracked Goland composite wing is investigated. The wing is modelled as a cantilevered beam based on Euler- Bernoulli equations. Also, composite material is modelled based on lamina fiber-reinforced. Edge crack is modelled by additional boundary conditions and local flexibility matrix in crack location, Castigliano's theorem and energy release rate formulation. Governing differential equations are extracted by Hamilton's principle. Using the separation of variables method, general solution in the normalized form for bending and torsion deflection is achieved then expressions for the cross-sectional rotation, the bending moment, the shear force and the torsional moment for the cantilevered beam are obtained. The cracked beam is modelled by separation of beam into two interconnected intact beams. Free vibration analysis of the beam is performed by applying boundary conditions at the fixed end, the free end, continuity conditions in the crack location of the beam and dynamic stiffness matrix determinant. Also, the effects of various parameters such as length and location of crack and fiber angle on natural frequencies and mode shapes are studied. Modal analysis results illustrate that natural frequencies and mode shapes are affected by depth and location of edge crack and coupling parameter.

• Steel and Composite Structures
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• v.43 no.5
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• pp.673-688
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• 2022

Post-earthquake fire is a major threat since most structures are designed allowing some damage during strong earthquakes, which will expose a more vulnerable structure to post-earthquake fire compared to an intact structure. A series of experimental research on steel-concrete composite beam-to-column joints subjected to fire after cyclic loading has been carried out and a clear reduction of fire resistance due to the partial damage caused by cyclic loading was observed. In this paper, by using ABAQUS a robust finite element model is developed for exploring the performance of steel-concrete composite joints in post-earthquake fire scenarios. After validation of these models with the previously conducted experimental results, a comprehensive numerical analysis is performed, allowing influential parameters affecting the post-earthquake fire behavior of the steel-concrete composite joints to be identified. Specifically, the level of pre-damage induced by cyclic loading is regraded to deteriorate mechanical and thermal properties of concrete, material properties of steel, and thickness of the fire protection layer. It is found that the ultimate temperature of the joint is affected by the load ratio while fire-resistant duration is relevant to the heating rate, both of which change due to the damage induced by the cyclic loading.