• 한방안이비인후피부과학회지
• /
• 제23권2호
• /
• pp.27-40
• /
• 2010

Background and Objective : Increasing interest in anti-aging and anti-wrinkling agents for the skin has triggered the recent outflow of researches and studies in this field. This study was designed to investigate the effects of bee venom on skin wrinkling and skin aging by testing the skin wrinkling, skin elasticity, trans-epidermal water loss (TEWL), free radical level, anti-oxidative agent level, and skin tissue after infusion of bee venom on hairless mouse. Materials and Methods : Fifteen hairless mice aged between 36~40 weeks were divided randomly into 3 Group; the Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group. The Bee Venom Syringe Group were injected subcutaneously with bee venom (0.1cc in total) using an insulin syringe on three spots in the lumbar spine (one spot on the center and two spots 1~2cm to the side bilaterally). The Bee Venom Needle Group were pricked with bee venom-smeared acupuncture needles on three longitudinal spots in the lumbar spine each 1cm apart, after which the needles were removed 10 minutes later. The Control Group did not receive any form of intervention. All procedures took place thrice a week for four weeks, during which the mice were allowed free access to water and fodder. The mice were measured and compared in the weight, skin wrinkling scale, skin elasticity, and TEWL before and after the experiment. After the experiment, blood samples were taken to measure the free radical and anti-oxidative agent level, and the skin tissue was sliced for examination. Data was analyzed using the SPSS program (ver 12.0). The ANOVA analysis was used to compare and contrast the three groups, and t-test for paired samples was used to evaluate skin-wrinkling before and after experiment. The cut-off p-value of significance was set at p<0.05. Results : 1. Administration of bee venom did not cause serious weight loss or gain. 2. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed a decrease in skin wrinkling scale after intervention. Especially, the Bee Venom Syringe Group showed a significant decrease (p<0.05). 3. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed an increase in skin elasticity. Especially, the Bee Venom Syringe Group showed a significant increase (p<0.05). 4. No significant change in TEWL was found in the mice in all the three groups before and after experiment. 5. Free radical level was normal in all 15 mice in all the three groups, and anti-oxidative agent was not significantly different across the three groups. 6. The Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group did not show any significant difference in the thickness of epidermis and dermis, infiltration of inflammatory cells, and skin wrinkling. The epidermis layer was relatively better preserved in the Bee Venom Syringe Group as compared to the Bee Venom Needle Group and the control group. Conclusion : Direct injection of bee venom on the hairless mouse using a syringe was found to improve wrinkling of the skin and increase skin elasticity but did not show effectiveness on skin dryness due to water loss. The bee venom appears to have suppressive effects on skin wrinkling, one of the symptoms of skin aging, through a process independent of suppression of free radicals or increase of anti-oxidative agent.

• Journal of Dairy Science and Biotechnology
• /
• 제33권1호
• /
• pp.35-46
• /
• 2015

최근 20년간 건강 및 질병 예방효과를 가진 식품에 대한 관심이 증가하고 있다. 오늘날 대다수의 노인들이 동물성 지방 과다 섭취로 인한 질환(예: 심혈관계 질병, 당뇨병)을 겪고 있으며, 이 질환으로 인한 세계 인구 사망률은 3번째로 높다. 최근 많은 사람들이 기능성 식품을 찾고 있으며, 기능성 식품은 모든 식품 부문, 그 중에서도 주로 낙농제품, 과자류, 청량음료, 제빵, 유아식 등의 부문에서 개발되고 있다. 식품 산업들이 새로운 식품분야에 도전함으로써 여러 가능성(예: 지방산 함유량의 재조리 또는 식이 섬유, 산화 방지제, probiotic 첨가를 통한 원료 및 가공물질의 성분 조절)을 시도해 볼 수 있게 되었다. 우유 및 유제품이 건강에 이로운 식품으로 인식되고 있으며, 우유에는 인체에 유익한 여러 가지의 생물적으로 활성화된 요소와 활성균이 함유되어 있다. 이 박테리아들은 우유에 존재하는 성분을 변형시킬 뿐만 아니라, 몇몇 심각한 질병을 완화시키며, 방부제 역할도 한다. 미생물의 상호 작용을 통해 발효된 유제품은 직접적으로 또는 발효과정중 생산된 비타민, 단백질, 펩타이드, 올리고당, 유기산 등의 대사산물을 통해 간접적으로 기능적 역할을 수행한다. probiotic 균이 면역반응(예: 사이토카인 및 항균 펩타이드 생산 조절)에 작용하는 기전을 토대로 이 박테리아들이 건강에 영향을 미치는 기전이 연구되고 있다. 유청 단백질은 항균 및 항발암 성질을 가지고 있으며, 여러 기능(예: 면역자극 생산, 건강 증진, 지방 침적 감소, 인슐린 감수성 향상등)을 가지고 있다. 유제품에 존재하는 박테리아와 특정 성분이 건강을 미치는 영향이 연구를 통해 밝혀지고 있다. 우유에 함유된 기능성 단백질, 생리활성, 펩타이드, 필수 지방산, 칼슘, 비타민 D 등의 물질은 면역체계, 심장혈관계, 위장관, 장 등의 건강을 증진시키며, 고혈압, 관상혈관질환, 비만, 골다공증, 암, 당뇨, 전달질병 등의 질환을 예방한다. 기능성 낙농식품에 함유된 성분들은 다양하게 응용되고 있다. 예를 들면, 카세인으로부터 파생된 포스포펩타이드가 식이보충제 및 제약보충제의 제조과정에 사용되고 있으며, 우유로부터 파생된 성장인자가 피부질환, 소화기계질환, 다리궤양, 건선, 내장, 골다공증 등을 치료하는 목적으로 사용되고 있다. 유제품을 통한 식이조절이 비만관리 및 예방에 도움이 될 가능성이 있으며, 건강을 증진시키는 질을 예방 및 치료 물질의 성분으로 사용될 것으로 여겨진다. 따라서 우유와 유제품이 기능성 식품 및 이 식품의 성분으로 사용될 가능성을 적극적으로 개발하는 연구가 절실히 요구되고 지속적으로 진행되어야 할 것이다.

• 대한한방내과학회지
• /
• 제15권1호
• /
• pp.22-44
• /
• 1994

Clinical observations were done on 67 cases with Diabetes Mellitus in CVA patients who were confirmed by CT scan and observed for over 1 week, admitted to the Dept. of Internal Medicine in Oriental Medical Hospital of Dongguk University from January 1992 to December 1993. The results were as follows; 1. 86 patients (15.3%) with Diabetes Mellitus were found in 561 CVA patients, the 6th decade of age was 40.2%, the ratio of male to female was 0.72:1. 2. The local distribution of CVA was similar to common CVA, and occlusive CVD was 83.6%, cerebral hemorrhage was 16.4% in this study. 3. The association between blood glucose and years were not significant. The largest ratio of fasting blood glucose were 140-199 mg/dl (44.6%) in admission, below 139 mg/dl (51.8%) in discharge in case of occlusive CVD. In cerebral hemorrhage, that were 140-199 mg/dl(45.5%) in dmission, below 139 mg/dl (45.5%) in discharge. The largest ratio of pp2hrs blood glucose were 200-299mg/dl in admission and discharge both occlusive CVD and cerebral hemorrhage. 4. The total sensitivity of urine glucose was 71.6%, and sensitivity of urine glucose in cerebral hemorrhage (81.8%) was more higher than that of occlusive CVD (69.6%). 5. Below 4 years had the highest prevalence(44.8%) in duration of diabetes mellitus. Patients usually used oral hypoglycemic agents(41.8%), insulin injection(23.9%) treatment and non-therapeutic was 17.9% in this study. 6. Predisposing factors and symptoms in admissin were similar to common CVA. The conscious disturbance on attacck was 41.1% in occlusive CVD, and that was 63.7% in cerebral hemorrhage. 7. The most common ratio of the season's attack was spring (44.8%), 8. The frequency of post history was as follows, hypertension (44.8%), heart disease (10.4%), and they were in below 199 mg/dl (83.3%) of fasting blood glucose. 9. The family history of CVA was 46.3%, and they was higher than nondiabetic patients. 10. The recurrence rate of CVA was 28.4%, and that of occlusive CVD(28.6%) was higer than cerebral hemorrhage's (18.2%). 11. The smoker was 52.2%, the drinker was 38.9%. 12. The complications was occured in 10 cases (14.9%) after admission, and they frequently occured than common CVA. 13. In admission, the ratio of systolic blood pressure in over l60mmHg was 42.9%, that of diastolic blood pressure in over l00mmHg was 12.5% in occlusive CVD. In cerebral hemorrhage, the ratio of systolic blood pressure in over l60mmHg was 54.5%, that of diastolic blood pressure in over l00mmHg was 27.3%. 14. The average beginned time of physical theraphy was, generally lated, 8.3 days in occlusive CVD, 11.2 days in cerebral hemorrhage. Average admitted period was longer than common CVA, and was 29.2 days in occlusive CVD, 11.2 days in cerebral hemorrhage. 15. The degree of recovery were 82.1% in occlusive CVD, 72.7% in cerebral hemorrhage. 16. The herb medications were various Sunghyanggeonggisans, Sopungtang, Ganghwalyupungtang, Yanggyuksan etc. were used most frequently, and Yukmijihwangtang, Gamidaebotang, Mangeumtang etc. were used as discharge.