• Title/Summary/Keyword: insects

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Molecular Mechanism of Plant Growth Promotion and Induced Systemic Resistance to Tobacco Mosaic Virus by Bacillus spp.

  • Wang, Shuai;Wu, Huijun;Qiao, Junqing;Ma, Lingli;Liu, Jun;Xia, Yanfei;Gao, Xuewen
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1250-1258
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    • 2009
  • Bacillus spp., as a type of plant growth-promoting rhizobacteria (PGPR), were studied with regards promoting plant growth and inducing plant systemic resistance. The results of greenhouse experiments with tobacco plants demonstrated that treatment with the Bacillus spp. significantly enhanced the plant height and fresh weight, while clearly lowering the disease severity rating of the tobacco mosaic virus (TMV) at 28 days post-inoculation (dpi). The TMV accumulation in the young non-inoculated leaves was remarkably lower for all the plants treated with the Bacillus spp. An RT-PCR analysis of the signaling regulatory genes Coil and NPR1, and defense genes PR-1a and PR-1b, in the tobacco treated with the Bacillus spp. revealed an association with enhancing the systemic resistance of tobacco to TMV. A further analysis of two expansin genes that regulate plant cell growth, NtEXP2 and NtEXP6, also verified a concomitant growth promotion in the roots and leaves of the tobacco responding to the Bacillus spp.

List of Intercepted Scale Insects at Korean Ports of Entry and Potential Invasive Species of Scale Insects to Korea (Hemiptera: Coccoidea) (우리나라 입항지에서 검출된 깍지벌레 목록 및 침입이 우려되는 깍지벌레(노린재목, 깍지벌레상과))

  • Suh, Soo-Jung;Yu, Hye Mi;Hong, Ki-Jeong
    • Korean journal of applied entomology
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    • v.52 no.2
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    • pp.141-160
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    • 2013
  • An updated list is given of 113 species of scale insects which have been intercepted on plants imported into Korea during the period of 2007 to 2011. Information on the number of interceptions, origin, host plants and distribution of species intercepted at Korean ports of entry is provided. In addition, data on intercepted species was analyzed to determine potential invasive species of scale insects that could threaten Korean plants.

Insect Adaptations to Changing Environments - Temperature and Humidity

  • Singh, Tribhuwan;Bhat, Madan Mohan;Khan, Mohammad Ashraf
    • International Journal of Industrial Entomology and Biomaterials
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    • v.19 no.1
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    • pp.155-164
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    • 2009
  • The most important factors in environment that influence the physiology of insects are temperature and humidity. Insects display a remarkable range of adaptations to changing environments and maintain their internal temperature (thermoregulation) and water content within tolerable limits, despite wide fluctuations in their surroundings. Adaptation is a complex and dynamic state that widely differs in species. Surviving under changing environment in insects depends on dispersal, habitat selection, habitat modification, relationship with ice and water, resistance to cold, diapause and developmental rate, sensitivity to environmental signals and syntheses of variety of cryoprotectant molecules. The mulberry silkworm (Bombyx mori) is very delicate and sensitive to environmental fluctuations and unable to survive naturally because of their domestication since ancient times. Thus, the adaptability to environmental conditions in the silkworm is quite different from those of wild insects. Temperature, humidity, air circulation, gases and photoperiod etc. shows a significant interaction in their effect on the physiology of silkworm depending upon the combination of factors and developmental stage affecting growth, development, productivity and quality of silk. An attempt has been made in this article to briefly discuss adaptation in insects with special emphasis on the role of environmental factors and their fluctuations and its significance in the physiology of mulberry silkworm, B. mori.

Insect Hormones and Their Actions (곤충의 호르몬과 작용)

  • 부경생
    • Korean journal of applied entomology
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    • v.40 no.2
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    • pp.155-196
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    • 2001
  • Basically insect hormones include ecdysteroids (molting hormone), juvenile hormones, and neurohormones comprising neuropeptides and biogenic amines. This article reviewed their chemical structures and biological functions. The active molting hormone is 20-hydroxyecdysone in most insects but makisterone A in some other insects including the honey bee and several phytophagous hemipterans. Most insects use JH III, but lepidopterans JH I and II. Dipterans also use a different JH, so-called JH $B_3$(JH III bisepoxide) and we still do not know the exact chemical structure of JH utilized in hemipterans. Some other insects use methyl farnesoate or hydroxylated JH III analogues as their juvenile hormone. Most diverse pictures can be found in neurohormones (NH), especially in neuropeptides, in terms of their number and structure. There are more than 200 neuropeptides (NP), classified into more than 30 families, which structures have been identified, and more of them are expected to be reported in the near future, partly due to rapid development in molecular biological techniques and in analytical techniques. More than half of them are involved in controlling activity of visceral muscles. But function (s) of many NPs are not clarified yet, even though their amino acid sequences have been identified. It is partly due to the fact that a single NP may have multiple functions. Another interesting point is their gene structure, having many number of independent, active peptides in one gene, apparently working for similar or totally different functions. NH also includes amines, such as octopamine, dopamine, serotonin, etc. From now on, investigation will be concentrated on identifying their function (s) and receptors, and on possibilities of their utilization as control agents against pest insects.

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Proteomics in Insecticide Toxicology

  • Park, Byeoung-Soo;Lee, Sung-Eun
    • Molecular & Cellular Toxicology
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    • v.3 no.1
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    • pp.11-18
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    • 2007
  • Mechanisms of insecticide resistance found in insects may include three general categories. Modified behavioral mechanisms can let the insects avoid the exposure to toxic compounds. The second category is physiological mechanisms such as altered penetration, rapid excretion, lower rate transportation, or increased storage of insecticides by insects. The third category relies on biochemical mechanisms including the insensitivity of target sites to insecticides and enhanced detoxification rate by several detoxifying mechanisms. Insecticides metabolism usually results in the formation of more water-soluble and therefore more readily eliminated, and generally less toxic products to the host insects rather than the parent compounds. The representative detoxifying enzymes are general esterases and monooxygenases that catalyze the toxic compounds to be more water-soluble forms and then secondary metabolism is followed by conjugation reactions including those catalyzed by glutathione S-transferases (GSTs). However, a change in the resistant species is not easily determined and the levels of mRNAs do not necessarily predict the levels of the corresponding proteins in a cell. As genomics understands the expression of most of the genes in an organism after being stressed by toxic compounds, proteomics can determine the global protein changes in a cell. In this present review, it is suggested that the environmental proteomic application may be a good approach to understand the biochemical mechanisms of insecticide resistance in insects and to predict metabolomic changes leading to physiological changes of the resistant species.

An Analysis of Ecological Habitat Characteristics in Medium-scale Stream -A Case of the Bokha Stream- (중규모 하천 생물 서식처의 특성 분석 -복하천을 중심으로-)

  • 안홍규;우효섭
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.2
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    • pp.102-119
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    • 2004
  • This study investigates the relation between the location of a habitat and the ecological connections according to the habitat type in the riparian zone at the Bokha stream. Stream habitat is classified into nine types for the aquatic insects and fish. For vegetation and birds, habitat is classified into five types of medium-scale streams, including both physical and chemical streams are analyzed accordingly. The major results of this study are as follows: 1) A pool connected to the main stream has a certain water depth (about 80 cm) and is disturbed at least 5 to 6 times a year. Although the pool has a somewhat bad water quality, it provides a habitat for various types of young fish. 2) A meander riffle is about 15 cm deep, the flow velocity in the region is about 75 cm/s. This region does not have large structures to which aquatic insects can attach, so it cannot be used as a spawning ground. 3) A rock type pool is about 60 cm deep, the flow velocity in the meander riffle is about 25 cm/s. In this region, many aquatic insects attached to large structures appear. Due to various riparian vegetation, the rock type pool is used as a habitat for aquatic insects. 4) A pool which is only connected to the main stream during floods has a bad water quality. Despite of its relatively small size, it has a high population density of aquatic insects. faculty.

Development of Chicken Breast Sausage with Addition of Mealworm (Tenebrio molitor Lavare) using Sensory evaluation

  • Kim, Youngkyun
    • International Journal of Internet, Broadcasting and Communication
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    • v.11 no.3
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    • pp.20-26
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    • 2019
  • The purpose of this study is to show probability of alternate food by using edible insects through the reports (Edible insects: Future prospects for food and feed security) edited by WFO (World Food Organization). We were carried out to develop the functional meat new product using Mealworm (Tenebrio molitor lavare) and Chicken breast. People's interest to the healthy, low-calories food is growing up, the Foodservice industry is developing and making Functional food, which helps to a sale strategy. Insects have played an important role as human food throughout history, especially in Africa, Asia and Latin America. A rapid increase in the human population is expected in the second half of the 21 century, which will lead to lower availability of food, especially animal protein As the problem of food supply and demand has come to the fore with climate change, Food and Agriculture Organization of the United Nations (FAO) has noticed edible insects as future food resources in order to prepare against the shortage of protein source. Recently consumers, especially patients have doubts about safety of raw materials for food. To overcome these limitations, I propose an enteral nutrition formula using edible insects as a raw material.

Analysis of Korean Patent Current Status Related to Food using Edible Insects (식용곤충을 이용한 식품 관련 국내 특허 현황 분석)

  • Park, Chanjeong
    • The Korean Journal of Food And Nutrition
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    • v.35 no.2
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    • pp.137-149
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    • 2022
  • The purpose of this paper was to analyze domestic patents for food using edible insects. From January 1, 1980 to December 31, 2021, patents filed with the Korean Intellectual Property Office were searched, and a total of 242 valid patents were selected. The trend of Korean patent applications for edible insect food has increased since 2015, with 57 cases (the highest number) in 2017. As for the edible insects used in food, Bombyx mori L. were the most common with 127 cases, followed by Tenebrio molitor L. with 118 cases. By type of applicant, individuals accounted for the most, with 132 cases. As a result of grouping patents by food classification, 67 cases of edible insects were used in snacks, breads, and rice cakes. As a result of patent analysis, an effort was made to supplement insufficient nutrients by adding nutritionally excellent edible insects to existing foods, and efforts were made to improve the sensory properties of insect foods. It is expected that this study will contribute to establishing future R&D directions and patent application strategies related to edible insect food.

Cyanogenic glycosides : Alternative insecticides?

  • Park, Dong-Sik;Coats, Joel R.
    • The Korean Journal of Pesticide Science
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    • v.6 no.2
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    • pp.51-57
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    • 2002
  • Cyanogenic glycosides are secondary plant metabolites that are known as plant defense chemicals. They are found in cassava, bamboo, flax, and other plants. In this paper, the role of cyanogenic glycosides, their characteristics, and their interactions with insects are discussed. Previous and current research in our laboratory found that several natural and synthetic cyanohydrins were effective against stored-product insects as fumigants. Due to their insecticidal activity to insects, cyanohydrins can be used as an alternative fumigant and also as soil fumigants. Risk assessment, however, should be done to account for possible environmental problems, non-target wildlife effects, and human health effects.

Enzyme Immunoassay for On-line Sensing of the Insecticide Imidaclopird Residues (살충제 이미다크로프리드 잔류물의 실시간 측정용 효소면역분석법)

  • 송석진;조한근
    • Journal of Biosystems Engineering
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    • v.28 no.6
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    • pp.505-510
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    • 2003
  • In Korea, due to its broad efficacy as a systemic insecticide, imidacloprid has been widely used in rice paddies to control sucking insects, soil insects, and some chewing insects and in apple orchards to control various insects pests. To quantify the imidacloprid residue concentrations, samples are assayed in vitro using enzyme-linked immunosorbent assays(ELISA). These assays generally require several hours to perform. As a biosensor, a competitive imidacloprid ELISA was modified to measure insecticide concentrations. It was found that a total assay time of 15 min(10-min antibody-antigen binding, and 5-min substrate development) is sufficient for monitoring imidacloprid concentrations. Further work is needed to improve the sensitivity of the measurement protocol.