• Journal of Korean Society of Forest Science
• /
• v.102 no.2
• /
• pp.170-175
• /
• 2013

This study was conducted to investigate the effect on the habitation of Monochamus alternatus, a vector insect of Pine wood nematode by the tended sound stumps for the developing forestry control methods of Pine wilt disease controls. Field cage plots (1 m ${\times}$ 1 m ${\times}$ 1 m) were established 12 units (Experiment1:4 units, Experiment2:8 Units) and were treated 4 kinds(February in 2010, February, April, June in 2011). The adults of M. alternatus were put into 4~5couples each the cage June in 2011. Presence or absence of the larva entrance holes and larva were detailed November in 2011. The larva were appeared in the sound stumps of sapling and young trees tended at February, April, June, the current emergence year of M. alternatus, but were not appeared in the sound stumps of sapling and young trees tended February in 2010, one year before the emergence of M. alternatus. The larva habitated in stumps of sapling were likely to be smaller in size in compare to the larva in stumps of young trees. In case of young tree, the larva were found at the cleft part of bark, but were not found at ridge part of bark. All observed larva appeared at part of bark and between the bark and the woody parts, but were not found at the woody parts. To develop the control guiding principal of sound stump after forest tending practice, further experiment need is in existence oviposition and larvae of vectors by height of stemps.

• Journal of Life Science
• /
• v.32 no.8
• /
• pp.601-610
• /
• 2022

P. tenebrifer (PT) belongs to the Diptera order and Stratiomyidae family. Recently, insect industry have been focused as food, animal feed and environmental advantages. γ-aminobutyric acid (GABA) and melatonin have been associated with regulating sleep and depression. GABA is the primary inhibitory neurotransmitter and is synthesized via biotransformation of monosodium glutamate (MSG) to GABA by lactic acid bacteria. In this study, we first used a GABA-enhanced PT extract, wherein GABA was enhanced by feeding MSG to PT. The underlying mechanisms preventing stress and insomnia were investigated in a corticosterone (CORT)-induced endoplasmic reticulum (ER) stress and chronic restraint stress (CRS)-exposed mouse model, as well as in pentobarbital (45 mg/kg)-induced sleep behaviors in mice. In the present study, the GABA peak was detected in high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis and showed in Ptecticus tenebrifer water extract (PTW) but not in non-PTW extract. The results showed that PTW and Ptecticus tenebrifer with 70% ethanol extract (PTE) exerted neuroprotective effects by protecting against CORT-induced downregulation of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and cAMP-response element binding protein (CREB) expression. In addition, PTW (300 mg/kg) significantly reduced CORT levels in CRS-exposed mice. Furthermore, PTW (100 and 300 mg/kg) significantly reduced sleep latency and increased total sleep duration in pentobarbital (45 mg/kg)-induced sleeping behaviors, which was related to serum melatonin levels. In conclusion, our results suggest that PTW exerts anti-stress and sleep-enhancing effects by regulating serum CORT and melatonin levels.

• Korean Journal of Environmental Biology
• /
• v.39 no.1
• /
• pp.119-135
• /
• 2021

Korea has stepped up efforts to investigate and catalog its flora and fauna to conserve the biodiversity of the Korean Peninsula and secure biological resources since the ratification of the Convention on Biological Diversity (CBD) in 1992 and the Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits (ABS) in 2010. Thus, after its establishment in 2007, the National Institute of Biological Resources (NIBR) of the Ministry of Environment of Korea initiated a project called the Korean Indigenous Species Investigation Project to investigate indigenous species on the Korean Peninsula. For 15 years since its beginning in 2006, this project has been carried out in five phases, Phase 1 from 2006-2008, Phase 2 from 2009-2011, Phase 3 from 2012-2014, Phase 4 from 2015-2017, and Phase 5 from 2018-2020. Before this project, in 2006, the number of indigenous species surveyed was 29,916. The figure was cumulatively aggregated at the end of each phase as 33,253 species for Phase 1 (2008), 38,011 species for Phase 2 (2011), 42,756 species for Phase 3 (2014), 49,027 species for Phase 4 (2017), and 54,428 species for Phase 5(2020). The number of indigenous species surveyed grew rapidly, showing an approximately 1.8-fold increase as the project progressed. These statistics showed an annual average of 2,320 newly recorded species during the project period. Among the recorded species, a total of 5,242 new species were reported in scientific publications, a great scientific achievement. During this project period, newly recorded species on the Korean Peninsula were identified using the recent taxonomic classifications as follows: 4,440 insect species (including 988 new species), 4,333 invertebrate species except for insects (including 1,492 new species), 98 vertebrate species (fish) (including nine new species), 309 plant species (including 176 vascular plant species, 133 bryophyte species, and 39 new species), 1,916 algae species (including 178 new species), 1,716 fungi and lichen species(including 309 new species), and 4,812 prokaryotic species (including 2,226 new species). The number of collected biological specimens in each phase was aggregated as follows: 247,226 for Phase 1 (2008), 207,827 for Phase 2 (2011), 287,133 for Phase 3 (2014), 244,920 for Phase 4(2017), and 144,333 for Phase 5(2020). A total of 1,131,439 specimens were obtained with an annual average of 75,429. More specifically, 281,054 insect specimens, 194,667 invertebrate specimens (except for insects), 40,100 fish specimens, 378,251 plant specimens, 140,490 algae specimens, 61,695 fungi specimens, and 35,182 prokaryotic specimens were collected. The cumulative number of researchers, which were nearly all professional taxonomists and graduate students majoring in taxonomy across the country, involved in this project was around 5,000, with an annual average of 395. The number of researchers/assistant researchers or mainly graduate students participating in Phase 1 was 597/268; 522/191 in Phase 2; 939/292 in Phase 3; 575/852 in Phase 4; and 601/1,097 in Phase 5. During this project period, 3,488 papers were published in major scientific journals. Of these, 2,320 papers were published in domestic journals and 1,168 papers were published in Science Citation Index(SCI) journals. During the project period, a total of 83.3 billion won (annual average of 5.5 billion won) or approximately US $75 million (annual average of US $5 million) was invested in investigating indigenous species and collecting specimens. This project was a large-scale research study led by the Korean government. It is considered to be a successful example of Korea's compressed development as it attracted almost all of the taxonomists in Korea and made remarkable achievements with a massive budget in a short time. The results from this project led to the National List of Species of Korea, where all species were organized by taxonomic classification. Information regarding the National List of Species of Korea is available to experts, students, and the general public (https://species.nibr.go.kr/index.do). The information, including descriptions, DNA sequences, habitats, distributions, ecological aspects, images, and multimedia, has been digitized, making contributions to scientific advancement in research fields such as phylogenetics and evolution. The species information also serves as a basis for projects aimed at species distribution and biological monitoring such as climate-sensitive biological indicator species. Moreover, the species information helps bio-industries search for useful biological resources. The most meaningful achievement of this project can be in providing support for nurturing young taxonomists like graduate students. This project has continued for the past 15 years and is still ongoing. Efforts to address issues, including species misidentification and invalid synonyms, still have to be made to enhance taxonomic research. Research needs to be conducted to investigate another 50,000 species out of the estimated 100,000 indigenous species on the Korean Peninsula.

• Korean Journal of Microbiology
• /
• v.46 no.1
• /
• pp.46-51
• /
• 2010

Coenzyme Q10 (CoQ10) is an essential lipid-soluble component of membrane-bound electron transport chains. CoQ10 is involved in several aspects of cellular metabolism and is increasingly being used in therapeutic applications for several diseases. Despite the recent accomplishments in metabolic engineering of Escherichia coli for CoQ10 production, the production levels are not yet competitive with those by fermentation or isolation. So we tested several microorganisms obtained from the KCTC of Biological Resource Center to find novel sources of strain-development for CoQ10-production. Then we selected two strains, Paracoccus denitrificans (KCTC 2530) and Asaia siamensis (KCTC 12914), and tested to optimize the CoQ10 production conditions. Among the carbon sources tested, CoQ10 production was the highest when fructose was supplied about 4% concentration. Yeast extract produced the highest CoQ10 production about 2% concentration. The highest CoQ10 production was obtained at pH 6.0 for P. denitrificans and pH 8.0 for A. siamensis. And two strains showed the highest CoQ10 production at $30^{\circ}C$, but the highest DCW was obtained at $37^{\circ}C$. In the fed-batch culture, P. denitrificans yielded $14.34{\pm}0.473$ mg and A. siamensis yielded $12.53{\pm}0.231$ mg of final CoQ10 production.

• Korean Journal of Plant Resources
• /
• v.8 no.2
• /
• pp.189-193
• /
• 1995

A new paeony variety 'Euiseongjakyak' was developed by Gyeongbug Provincial Rural Development Administration in 1993. This variety was selected from pure lint selection. It is characterized by red flower of single type, deep white flesh colour, straight and big radixs. When compared with 'Punggi jerye' a check variety, it is more resistant to lodging, disease and insect injury, and outyielded by 32% in total radix and 17% in marketable radix during 3 years regional performance trials. In the radix, the content of starch and sugar was higher by $9.8\sim12.22%$, crude protein and ash was similar and fiber was lower by 1.17% compared to 'Punggi jerye'.

• Korean Journal of Environment and Ecology
• /
• v.29 no.3
• /
• pp.368-373
• /
• 2015

Faecal analysis was carried out to identify the diet composition of Eptesicus serotinus after collecting the faeces from 25 female bats. Five bats were captured at a bridge where a colony of E. serotinous was found in Andong city, Gyeongbuk, every month from May to September 2014. A total of 100 faeces were collected and the parts of insects obtained from the faeces were examined using a high-resolution microscope (50 ${\times}$ binocular microscope) and then the orders of the insects were identified by the characteristics of the parts of the insect (mouthpart, leg, wing, antenna, elytra, etc.). According to the results of the faecal analysis, 4 orders: Coleoptera, Hymenoptera, Diptera and Hemiptera were used as food resource of E. serotinus. Coleoptera was found in May, July, August, and September. Interestingly, only Coleoptera were found in May and July. Hemiptera, Diptera, and Hymenoptera were found to be used as food resources in September, June, and August.

• Korean Journal of Plant Resources
• /
• v.19 no.4
• /
• pp.488-490
• /
• 2006

In double cropping fields, there has been a risk of increase rice stripe viral disease outbreaking as the vector, small brown planthopper that overwinter in barley fields increases. Especially rice cultivation in Honam region has two types of cropping system such as rice only cropping in a season and double cropping both of rice and barley. In double cropping system, harvesting of barley could induce migration of the vector from barley to rice field. We found that the vector's density was different in rice fields by distance from barley field. The vector's density in rice field was highest 63 numbers within one meter from the neighbor barley field, and the numbers reduced greatly as the distance from barley field increases. The disease incidence according to rice cultivation methods was different, Donjin 1 was appeared to be a susceptible variety showing 2.4% and 2.5% of disease incidence in direct and transplanting cultivation, respectively. The small brown planthopper in a nearby rice field reach peak 3 days after the barley field was harvested.

• Korean Journal of Plant Resources
• /
• v.35 no.1
• /
• pp.10-22
• /
• 2022

This study investigated field data to understand the spatio-temporal distribution of pollinating insects and buckwheat flowers. We set the in-situ observation sites in different locations to get altitude and cropping system distribution data for five years (2016 to 2020) in Korea. Twenty-five different insect species, belonging to 8 orders, were recorded. Over the past five years, species from the orders Diptera and Hymenoptera were the principal visitors. Hymenoptera was mainly represented by honey bees (Apis cerana), while Diptera was represented by bean seed fly (Delia platura) and several other species. Some bees and other Hymenoptera species could, however, act as co-pollinators because of their high relative frequency and activity. Compared with open-field cultivation (conventional), the pollination mediating effect of flies and bees was superior in net house, so the yield was high, and it was also found to be slightly higher in the mixed treatment of flies and bees than in the single treatment. Based on the above results, flies and bees were found to be the most active pollinating insects in buckwheat and it is necessary to actively utilize the selected insects to improve buckwheat productivity. This relationship will be utilized in establishing the system of seed production on pollinating regulation of a primary plant.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.18 no.3
• /
• pp.31-37
• /
• 2010

Chitinases (EC 3.2.1.14) hydrolyze the ${\beta}$-1,4-linkages in chitin, the second most abundant polymer of N-acetyl-${\beta}$-D-glucosamine which is a structural component of protective biological matrices such as fungal cell walls and insect exoskeletons. The glycosyl hydrolases 18 family including chitinases is an ancient gene family widely expressed in archea, prokaryotes and eukaryotes. Since earthworms live in the soil with a lot of microbial activities and fungi are supposed to be a major component of the diet of earthworm, it has been reported that there would be appropriate immune system to protect themselves from microorganisms attacks. In this study, the novel chitinase, EaChi, from the midgut of earthworm, Eisenia andrei, were identified and characterized. To obtain full-length cDNA sequence of chitinase, RT-PCR and RACE-PCR analyses were carried out by using the previously identified EST sequence amongst cDNA library established from the midgut of E. andrei. EaChi, a partial chitinase gene, was composed of 927 nucleotides encoding 309 amino acids. By the multiple sequence alignments of amino acids with other different species, it was revealed that EaCHI is a member of glycosyl hydrolases 18 family, which has two highly conserved domains, substrate binding and catalytic domain.

• Korean journal of applied entomology
• /
• v.48 no.2
• /
• pp.237-243
• /
• 2009

This study was conducted to develop economic injury level (ElL) and economic threshold (ET) of Cabbage armyworm, Mamestra brassicae L. on cabbage (Brassica oleracea L. var). The changes of cabbage biomass and M. brassicae density were investigated after introduction of larval M. brassicae (2nd instar) at different densities: 0, 1, 2, 4, 8, and 16 larvae per plant at 40 d after planting for an open field experiment, and 0, 2, 5, 8 and 12 larvae per plant at 25 d after planting for a glass house experiment. In the field experiment, the yield loss of cabbage was not significantly different among treated-plots at 30 d after the larval introduction, showing an over-compensatory response of cabbage plants to M. brassicae attack. In the glasshouse experiment, however, the biomass of cabbage at 15 d after the larval introduction significantly decreased with increasing the initial introduced number of M. brassicae, resulting in 38.3, 36.7, 21.7, 23.3 and 16.7g in above treated-plots, respectively. The relationship between cumulative insect days (CID) and yield loss (%) of cabbage was well described by a nonlinear logistic equation. Using the estimated equation, ElL of M. brassicae on cabbage was estimated at 44 CID per plant based on the yield loss 14%, which take into account of an empirical gain threshold 5% and marketable rate 91% of cabbage. Also, ET was calculated at 80% of the EIL: 35 CID per plant. Until a more elaborate EIL-model is developed, the present result may be useful for M. brassicae management at early growth stage of cabbage.