• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.85-85
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• 2005

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2016.09a
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• pp.156-160
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• 2016

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.113-113
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• 2002

• Journal of Forest and Environmental Science
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• v.36 no.4
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• pp.326-327
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• 2020

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.394-398
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• 2017

A survey to assess the diversity of wound-associated Ophiostomatales and Microascales, the so-called ophiostomatoid fungi, on Korean native trees, was undertaken in 2017. Wounds were artificially created, and a fungus resembling a species of Sporendocladia was consistently isolated from the exposed cambium and inner bark of artificially induced wounds on Quercus variabilis. Morphological examination and DNA sequence comparisons based on the internal transcribed spacer (ITS) and 5.8S regions of the rDNA confirmed the identity of the fungus as Sporendocladia bactrospora. This is the first report on S. bactrospora occurring on Q. variabilis in Korea.

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Plant Pathology Journal
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• v.37 no.5
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• pp.437-445
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• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.208-218
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• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• Korean journal of applied entomology
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• v.57 no.1
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• pp.33-38
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• 2018

Walnuts are one of the most economically important fruit trees in forestry. In walnut orchards, however, studies examining damage by insect pests are limited. This study was conducted to investigate the rate of damage caused by Conogethes punctiferalis to walnut fruits and by Acrocercops transecta to walnut leaves. In 2017, the change of damage rate (caused by C. punctiferalis) to fruits were investigated in Buyeo. In addition, we counted fruits and leaves damaged by two lepidopteran species in Gimcheon, Buyeo, Yeongdong, and Hwaseong. Damaged fruits steadily increased in abundance with fruit growth in Buyeo, and the highest number of damaged fruits was documented in late summer. The rates of damage caused by C. punctiferalis to fruits in Buyeo, Gimcheon, and Hwaseong were 22.1%, 20.5%, and 11.7%, respectively. Additionally, the rates of damage caused by A. transecta to leaves in seedlings (58.5% on average) were significantly higher than to those in older trees (11.2% on average), irrespective of the study location. In conclusion, this study showed that C. punctiferalis and A. transecta were serious pests affecting walnut fruits and leaves, respectively. Therefore, management methods for these insect pests need to be developed.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.