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The Use of Styrofoam Box for Chikso (Korean Brindled Cattle) Semen Cryopreservation with Liquid Nitrogen (칡소 동결 정액 생산을 위한 스티로폼상자와 액체질소 이용 방법)

  • Kim, Sung Woo;Ko, Yeoung-Gyu;Lee, Jae-Yeong;Kim, Chan-Lan;Hwang, In-Sul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.490-496
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    • 2020
  • A styrofoam box is used as a simple and easy freezing method to preserve animal semen as a livestock genetic source. This study optimized the methods of freezing chikso brindled cattle semen. To test the freezing box, the motility of spermatozoa was compared between two box sizes (length×width×heigh) with the dimensions of 23.5×30.5×22.5 cm and 25.5×46.5×26.5 cm. The motility of thawed sperm from brindled Korean bulls was used to confirm the efficiency of the freezing boxes. The box having a larger inner space with larger horizontal and height measurements supported better motility after thawing (60.4±5.3% vs 67.2±3.1%) with 10 min of exposure time in liquid nitrogen vapor. The optimized freezing space is estimated to be an essential element for successful freezing results and the larger box could be used for production of more than 60 frozen semen straws. These properties are also helpful to optimize the cryopreservation techniques that would control the quality and quantity of semen straws according to different animal species.

Morphological Studies on Hair Growth Effect of Samultang-gamibang (사물탕가미방(四物湯加味方)이 원형탈모 생쥐의 발모효과에 관한 형태학적 연구)

  • Kwon, Gang-Joo;Lee, Chang-Hyun;Lee, Moon-Won;Mun, Yeun-Ja;Choi, Doo-Ho;Lee, Hak-Jae;Kim, Ho-Min;Woo, Won-Hong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.3
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    • pp.696-704
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    • 2005
  • This experiment examined the effect of Samultang-gamibang (SGB) on hair growth in spontaneous alopecia areata C57BL/6N mice. We first investigated hair growth effect of SGB compare to control groups after apply to oral administration for 10 weeks and regional treatment in skin for last 4 weeks. We second investigated the number of hair follicle and mast cells after treatment of SGB in spontaneous alopecia areata C57BL/6N mice for 10 weeks. We third investigated immunoreactive density of neuropeptides in skin of spontaneous alopecia areata C57BL/6N mice by immunohistochemical methods. The results were as follows : Hair growing effect of experimental group was observed from 7 weeks after administration of SGB (87.5%). In experimental group, the number of mast cells and eosinophils was significantly decreased compare to control group. Immunoreactive density of substance P and corticotropin releasing factor (CRF) in skin of experimental group was weakly stained in epidermis and subcutaneous tissue compare to control group. Immunoreactive density of CRF-receptor (CRF-R), CRF-binding protein (CRF-BP) in skin of experimental group was increased in epidermis, sebaceous gland, inner root sheath, outer root sheath and secondary hair germ epithelium compare to control group. These results suggest that SGB may be used in treatment of alopecia areata.

Characteristics and Yield of Jochung Processed by Different Preparation Methods (제조 방법에 따른 쌀 조청의 특성 및 수율)

  • Choi, Yoon-Hee;Baek, Ji-Eun;Park, Shin-Young;Choi, Hye-Sun;Song, Jin
    • The Korean Journal of Food And Nutrition
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    • v.27 no.3
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    • pp.414-420
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    • 2014
  • This study was performed to increase the yield and to reduce the processing times for the preparation to improve the productivity and quality of rice jochung, a traditional food in Korea. In order to evaluate the quality characteristics and yield of jochung, the viscosity, color value, mineral contents and the sensory evaluation were measured. Jochung is prepared from steamed rice (STR), wet-milled rice flour (WRF) and dry-milled rice flour (DRF) by processing methods of rice and reacting times (6 hours or 13 hours) of liquefaction and saccharification. There is commonly added liquefying enzyme for rice liquefaction (0.4%/10 kg rice, at $85{\sim}90^{\circ}C$ for 1 hour or 4 hours) and saccharogenic enzyme with malt (2.5% or 4.5%/10 kg rice, at $56{\sim}60^{\circ}C$ for 5 hours or 9 hours). The inner structural properties of WRF showed the more distinct shape regular structure of uncombined starch particles but the DRF closely maintained particles of rice flour observed by SEM. If processing times for liquefation and saccharification were reduced from 13 hours to 6 hours, the yield of jochungs prepared with WRF increased 8%, the DRF 7%, and the STR 3% respectively and the sensory evaluation as well as color values and overall desirability received high scores. The viscosity, color a and b values of jochung processed with WRF for 6 hours were lower than that processed for 13 hours. The viscosity and color a, b value and Ca content were decreased in the jochung processed with WRF or DRF for 6 hours, but Mg, P and K were increased than that of STR. Jochung processed by 0.4% liquefying enzyme and 2.5% malt with WRF for 6 hours will increase the yield, save manufacturing times and costs and will thereby enable cost-effective techniques.

Late Quaternary Seismic Stratigraphy and Sedimentation of the Southeastern Continental Shelf, Korea Strait (한국 남동해역(대한해협) 대륙붕지역의 후 제4기 탄성파 층서 및 퇴적작용)

  • Yoo Dong-Geun;Lee Chi-Won;Min Gun-Hong;Lee Ho-Young;Choi Joung-Gyu;Park Soo-Chul
    • Geophysics and Geophysical Exploration
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    • v.8 no.3
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    • pp.201-206
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    • 2005
  • Interpretation of high-resolution seismic profiles from the southeastern continental shelf of Korea reveals that the sedimentary deposits consist of seven seismic units formed during the late Quaternary. These units comprise lowstand, transgressive, and highstand systems tracts. The lowstand systems tract consists of a lowstand prograding wedge (SU1) and a mass flow deposit (SU2) including slumps and slides. The transgressive systems tract contains four seismic units: an ancient beach/shoreface deposit (SU3), a channel-fill deposit (SU4), a transgressive sand layer (SU5), and a transgressive sand ridge (SU6). The highstand systems tract consists of an inner-shelf mud deposit (SU7) derived from the Nakdong and Seomjin rivers during the last 6 ka when sea level was close to the present level.

Controlled Bandwidth Borrowing with Extended RSVP-TE to Maximize Bandwidth Utilization

  • Kim Chul;Kim Young-Tak
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.1B
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    • pp.64-72
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    • 2004
  • Multiprotocol Label Switching (MPLS) has been developed as a key technology to enhance the reliability, manageability and overall quality of service of core If networks with connection-oriented tunnel LSP and traffic engineering such as constraint-based routing, explicit routing, and restoration. In this paper, we propose a control bandwidth borrowing scheme that maximizes the utilization of tunnel LSPs or physical links by an extension to the RSVP-TE label distribution protocol. MPLS-based core switching network and VPN services rely on the establishment of connection-oriented tunneled LSPs that are configured or predefined by network management systems. The mechanism of network management system varies from (i) a relatively static LSP establishment accounting, to (ii) a dynamic QoS routing mechanisms. With the use of hierarchical LSPs, the extra bandwidth that is unused by the trunk (outer) LSPs should be fully allocated to their constituent end-to-end user traffic (inner) LSPs in order to maximize their utilization. In order to find out the unused extra bandwidth in tunnel LSP or physical link and redistribute these resources to constituent LSPs, we expend the functionality of RSVP-TE and the found unused extra bandwidth is redistributed with a weight-based recursive redistribution scheme. By the extended RSVP-TE and proposed recursive redistributed scheme, we could achieve the instantaneous maximized utilization of tunnel LSP or physical link suffering from the potential under-utilization problem and guarantee the end-to-end QoS requirements. With the proposed scheme, network manager can manage more effectively the extra available bandwidth of hierarchical LSPs and maximize the instantaneous utilization of the tunneled LSP resources.

The Distribution of Flora and Management Status for the Inner Windbreak Forests at Jangsoo-ri and Pyeongsa-ri in Yeosu City (여수시 장수리, 평사리 방풍림일대의 식물분포와 관리실태)

  • Oh, Hyun-Kyung;Kim, Yong-Shik;Kim, Do-Gyun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.1
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    • pp.44-56
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    • 2007
  • The flora was summarized as 195 taxa; 73 families, 138 genera, 168 species, 23 varieties and 4 forms in Jangsoo-ri windbreak forest and 151 taxa; 63 families, 118 genera, 131 species, 18 varieties and 2 forms in Pyeongsa-ri windbreak forest. Based on the list of the rare plants by the Forest Research Institute, 3 taxa and based on the list of Korean endemic plant 4 taxa were recorded in the studied areas; Puccinellia coreensis (Gramineae), Asarum maculatum (Aristolochiaceae), Prunus yedoensis (Rosaceae), Koelreuteria paniculata (Sapindaceae), Adenophora triphylla var. hirsuta (Campanulaceae). The Specific Plant Species which is categorized by the Ministry of Environment, Korea, was summarized as 23 taxa for the 1 degree of 17 taxa, 3 degree of 5 taxa, 5 degree of 1 taxa in Jangsoo-ri windbreak forest and 22 taxa for the 1 degree of 18 taxa, 3 degree of 3 taxa, 5 degree of 1 taxa in Pyeongsa-ri windbreak forest. From the enlisted the flora surveyed, the naturalized plants were 17 taxa in Jangsoo-ri windbreak forest and 19 taxa in Pyeongsa-ri windbreak forests with the Naturalization rate was 8.7 percent and 12.6 percent, respectively. In Jangsoo-ri windbreak forest, there has been occurred many man-made impair and development without legal institution and control. So, it needs in haste institutional conservation measures and systemic database construction about function and necessity of windbreak forest. In Pyeongsa-ri windbreak forest, recently many people visited here. So, if we make eco-tour place connecting nearby coast and windbreak forest, it will contribute resident's economic profit and opportunity, concerns about windbreak forest, and necessity of conservation measures. Recently, the windbreak forests in Jangsoo-ri and Pyeongsa-ri should be bereaved windbreak forest of function by the human activities.

Development of RT-PCR Kit for Diagnosis of Pathogenic Agent of Ginseng Root Rot in the Ginseng Field (인삼포장에서 뿌리섞음병원균의 진단을 위한 RT-PCR KIT의 개발)

  • 도은수
    • Korean Journal of Plant Resources
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    • v.16 no.1
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    • pp.40-48
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    • 2003
  • Cylindrocarpon destructans is the major pathogen inducing the root rot disease in ginseng. Up to now, there is no reliable and convenient method to analyze the spore density or population of this pathogen in ginseng-growing soil or any contaminated farmlands. Therefore, it will be very valuable to develop a new and reliable method in detecting the spore of this pathogen. In this study, a molecular biological technique using two step nested PCR method, was developed. Two universal ITS primers, ITS5F and ITS4R were used in the first round of PCR to amplify a fragment of ITS region from the genomic DNA of C. destructans. The specific prmers Nest 1 and Nest 2 were designed and used in the second round of PCR to amplify a inner fragment from the first round PCR product of C. destructans. C. destructans spore, only soil samples from the diseased ginseng farm produced the positive bands, suggesting its usefulness in detecting the C. destructans spores in soil samples. Thus it is recommended to first extract the whole genomic DNA from soil samples and use it for the PCR reaction, thereby eliminating the inhibitory activity of soil components.

A Study on Pore Structure of High-Fluidity Concrete using Lime Stone Powder and Fly-ash (석회석 미분말 및 플라이 애시를 사용한 고유동 콘크리트의 공극구조에 관한 연구)

  • Choi, Yun-Wang;Hooton, R.D.;Eom, Joo-Han
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.3
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    • pp.118-125
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    • 2011
  • The size and distribution of concrete void was one among the factor determining durability of concrete. Recently, there was a lot of researches related to the High-Fludity Concrete(HFC) with field applications. However, the research about the void structure having an effect on durability of concrete is insufficient. Therefore, in this research, Conventional Concrete(CC) and HFC using lime stone powder and fly-ash of 30 MPa range was manufactured and observed the void structure of CC and HFC. Experimental results showed that average pore diameter in the case of the 30 MPa range HFC was to be lower than CC and SEM analyzed result, HFC was firmer inner structure than CC.

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Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection

  • Yoo, Jinhee;Park, Kimoon;Yoo, Youngji;Kim, Jongkeun;Yang, Heejin;Shin, Youngjae
    • Food Science of Animal Resources
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    • v.34 no.1
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    • pp.26-32
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    • 2014
  • This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN-${\gamma}$ caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN-${\gamma}$-induced inflammation, anti-collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.

The Reducing Effects Analysis of Floods through Washland Construction in Hwapocheon Basin (화포천 유역의 천변저류지 조성을 통한 홍수 저감효과 분석)

  • Jeong, Young-Won;Kim, Young-Do;Park, Jae-Hyun;Yoon, Byung-Man
    • Proceedings of the Korea Water Resources Association Conference
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    • 2009.05a
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    • pp.1489-1493
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    • 2009
  • The disaster with many casualties every year by floods, and the economic loss will occur in Korea. The establishment and the recovery measures are necessary. In this research, we analyzed the effect for reducing flood by making washland in flood season, where is used as the wetland in non-flood season in Hwapocheon basin of Nakdong River, Korea. We prepared draingage of inner basin for flood in the past because the water elevation of Hwapocheon is lower than the water elevation of the Nakdong River. On the other hand, now a days, drainage capacity of the expansion and change of the height of the embankment have limitations, because of the increase in torrential rains. In this study, HEC-RAS is used for the unsteady flow routing for the effectiveness analysis of flood level mitigation in flood season. This analysis was performed according to the scenarios of washland construction location and its scale.

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