• Title/Summary/Keyword: initial seed

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Prediction Model for the Extraction Weights and Extraction Rate of Barley and Cassia Tora Seed Tea by Different Extraction Conditions (보리차 및 결명자(決明子)차의 추출조건(抽出條件)의 변화(變化)에 따른 추출량(抽出量) 및 추출속도(抽出速度) 예측(豫測)모델)

  • Jeong, Mun Ho;Choi, Yong Hee
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.95-106
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    • 1990
  • The most important factors among extraction conditions in the extraction process of Barley and Casia tora seed are particle size, extraction temperature, time and initial concentration. In this research project, then, the amounts of extracted materials were measured at various conditions of above factors. They were increased as the particle sizes were decreased and were also increased in the proportional to the value of square of temperature. General mathematical prediction models were developed by an optimization technique for the amounts of extracted materials and extraction rate on the basis of each independent factor. Then, the final prediction model was obtained upon all the factors. As the results, it was also found that the values of overall mass transfer coefficients were increased as the particle sizes were decreased.

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CaCO3 Biomineralization in Microfluidic Crystallizer (미세유체 결정화기를 이용한 탄산칼슘 Biomineralization)

  • Seo, Seung Woo;Ko, Kwan Young;Lee, Chang Soo;Kim, In Ho
    • Korean Chemical Engineering Research
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    • v.51 no.1
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    • pp.151-156
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    • 2013
  • Crystallization of $CaCO_3$ is practiced on a polymethylsiloxane (PDMS) - based microfluidic system. Liquid- liquid reaction was investigated by mixing calcium chloride ($CaCl_2$) and sodium carbonate ($Na_2CO_3$) solution to crystallize $CaCO_3$. Aspartic acid (Asp) was added to investigate the morphology change such as vaterite and calcite. Suitable ratio of $Na_2CO_3$ and $CaCl_2$ was searched for initial seed formation. Christmas tree model was used as microfluidic device to form concentration gradient of $Na_2CO_3$ and $CaCl_2$. After observing microfluidic channel by using optical microscope, we found that seeds of $CaCO_3$ were formed under the condition that the ratio of $Na_2CO_3$ and $CaCl_2$ was 2:1. Morphology of crystals were also observed as $CaCO_3$ crystals grow. When Asp was added, vaterite crystal was more frequently found in two morphologies (vaterite and calcite) and seed formation and crystal growth were inhibited.

Enhanced Seed Development in the Progeny from the Interspecific Backcross (Fagopyrum esculentum ${\times}$ F. homotropicum) ${\times}$ F. esculentum

  • Shin, Dong-Hoon;Kamal, A H M;Yun, Young-Ho;Bae, Jeong-Sook;Lee, Yun-Sang;Lee, Moon-Soon;Chung, Keun-Yook;Woo, Sun-Hee
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.209-214
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    • 2009
  • To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The $F_{2}$ generation was more amenable than $F_{1}$ hybrids to produce backcross progenies. The $F_{1}$ hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BC$F_{1}$ ${\times}$ F. esculentum (thrum) and F. homotropicum ${\times}$ BC$F_{1}$ was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between $F_{1}$ plants and selected fertile plants of the second congruity backcrosses.

Molecular cloning, expression and characterization of a squalene synthase gene from grain amaranth (Amaranthus cruentus L.)

  • Park, Young-Jun;Nemoto, Kazuhiro;Matsushima, Kenichi;Um, Han-Yong;Choi, Jung-Hoon;Oh, Chan-sung;Nishikawa, Tomotaro
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.89-89
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    • 2017
  • A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All of the exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained conserved six domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was low levels of SQS transcripts at the initial stage of seed development, but the levels increased rapidly at the mid-late developmental stages before declining at the late developmental stage. These findings showed that the amaranth SQS is a late-expressed gene that is rapidly expressed at the mid-late stage of seed development. In addition, we observed that the SQS mRNA levels in stems and roots increased rapidly during the four- to six-leaf stage of development. Therefore, our results showed that the expression levels of SQS in stem and root tissues are significantly higher than those in leaf tissues. In present study provides useful information about the molecular characterization of the SQS clone isolated from grain amaranth. Finally, a basic understanding of these characteristics will contribute to further studies on the amaranth SQS.

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A Study on Seed Germination and Seedling Growth of Cornus kousa Treated with Livestock Manure (가축분뇨 처리에 의한 산딸나무 종자발아 및 유묘생육에 관한 연구)

  • Lee, Chang-Heon;Kang, Hag-Mo;Kim, Mi-Ja
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.2
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    • pp.105-118
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    • 2015
  • This study was conducted to obtain proper amount of solid swine manure and poultry manure in the beginning phase of Cornus kousa growth by investigating the initial germination, seedling growth increment, dry weight, chlorophyll content change, body nutrient uptake and chemical changes of soil according to the concentration of solid swine manure and poultry manure fertilization. When treated with solid swine manure and poultry manure, seed germination rate was the highest in the control. However germination rate showed a tendency to decrease when treated with high concentration of swine manure and poultry manure. The survey results of the growth increment were all higher than that of the control. At the 1.0% treatment of both swine and poultry manure, the seedling height growth was highest. Poultry manure treatment made higher result than swine manure treatment at each treatment. Dry weight was the highest at the 1.0% treatment by both swine and poultry manure. Dry amount declined sharply at the 2.0% treatment. Poultry manure was higher in weight than swine manure at every treatment. Chlorophyll content was the highest at the 1.0% treatment by both swine and poultry manure, but declined sharply at the 2.0% treatment. The survey content was higer than that of the control. The amount of nutrients absorbed in the seedling body was generally high at the 1.0% treatment of swine manure and poultry manure. For the planting soil of Cornus kousa the higher concentration of swine manure and poultry manure was, the lower pH became. However, nitrogen, got higher. available phosphoric acid, exchangeable K, Na, and Mg got higher.

Fabrication of Highly Efficient Nanocrystalline Silicon Thin-Film Solar Cells Using Flexible Substrates (유연기판을 이용한 고효율 나노결정질 실리콘 박막 태양전지 제조)

  • Jang, Eunseok;Kim, Sol Ji;Lee, Ji Eun;Ahn, Seung Kyu;Park, Joo Hyung;Cho, Jun-Sik
    • Current Photovoltaic Research
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    • v.2 no.3
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    • pp.103-109
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    • 2014
  • Highly efficient hydrogenated nanocrystalline silicon (nc-Si:H) thin-film solar cells were prepared on flexible stainless steel substrates using plasma-enhanced chemical vapor deposition. To enhance the performance of solar cells, material properties of back reflectors, n-doped seed layers and wide bandgap nc-SiC:H window layers were optimized. The light scattering efficiency of Ag back reflectors was improved by increasing the surface roughness of the films deposited at elevated substrate temperatures. Using the n-doped seed layers with high crystallinity, the initial crystal growth of intrinsic nc-Si:H absorber layers was improved, resulting in the elimination of the defect-dense amorphous regions at the n/i interfaces. The nc-SiC:H window layers with high bandgap over 2.2 eV were deposited under high hydrogen dilution conditions. The vertical current flow of the films was enhanced by the formation of Si nanocrystallites in the amorphous SiC:H matrix. Under optimized conditions, a high conversion efficiency of 9.13% ($V_{oc}=0.52$, $J_{sc}=25.45mA/cm^2$, FF = 0.69) was achieved for the flexible nc-Si:H thin-film solar cells.

Waterborne Core-shell Pressure Sensitive Adhesive (PSA) Based on Polymeric Nano-dispersant (고분자 분산제를 이용한 Core-shell 수성 감압점착제)

  • Lee, Jin-Kyoung;Chin, In-Joo
    • Journal of Adhesion and Interface
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    • v.17 no.3
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    • pp.89-95
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    • 2016
  • An environmentally friendly water-based pressure sensitive adhesive (PSA) was designed in an attempt to replace the solvent-based adhesive for dry lamination used in flexible food packaging films. Instead of using a low molecular weight surfactant, which may have variable material properties, a high molecular weight dispersant was used for emulsification. A polymeric nano-dispersant (PND) was synthesized using solution polymerization, and it was used as a micelle seed in the surfactant, resulting in the synthesis of a core/shell grafted acrylic adhesive. The shell and core exhibited different $T_g$ values, so that the initial adhesion strength and holding power were complemented by the film's flexibility, which is required to provide good adhesion of thin films. Results showed that the PSA designed in this study using the PND instead of traditional low molecular weight surfactant had adhesive properties applicable to the flexible packaging with appropriate tack.

Growth behavior on initial layer of ZnO:P layers grown by magnetron sputtering with controlled by $O_2$ partial pressure

  • Kim, Yeong-Lee;An, Cheol-Hyeon;Bae, Yeong-Suk;Kim, Dong-Chan;Jo, Hyeong-Gyun
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.28.1-28.1
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    • 2009
  • The superior properties of ZnO such as high exciton binding energy, high thermal and chemical stability, low growth temperature and possibility of wet etching process in ZnO have great interest for applications ranging from optoelectronics to chemical sensor. Particularly, vertically well-aligned ZnO nanorods on large areas with good optical and structural properties are of special interest for the fabrication of electronic and optical nanodevices. Currently, low-dimensional ZnO is synthesized by metal-organic chemical vapor deposition (MOCVD), molecular beam epitaxy (MBE), thermal evaporation, and sol.gel growth. Recently, our group has been reported about achievement the growth of Ga-doped ZnO nanorods using ZnO seed layer on p-type Si substrate by RF magnetron sputtering system at high rf power and high growth temperature. However, the crystallinity of nanorods deteriorates due to lattice mismatch between nanorods and Si substrate. Also, in the growth of oxide using sputtering, the oxygen flow ratio relative to argon gas flow is an important growth parameter and significantly affects the structural properties. In this study, Phosphorus (P) doped ZnO nanorods were grown on c-sapphire substrates without seed layer by radio frequency magnetron sputtering with various argon/oxygen gas ratios. The layer change films into nanorods with decreasing oxygen partial pressure. The diameter and length of vertically well-aligned on the c-sapphire substrate are in the range of 51-103 nm and about 725 nm, respectively. The photoluminescence spectra of the nanorods are dominated by intense near band-edge emission with weak deep-level emission.

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Silymarin seed extract supplementation enhances the growth performance, meat quality, and nutrients digestibility, and reduces gas emission in broilers

  • Shanmugam, Sureshkumar;Park, Jae Hong;Cho, Sungbo;Kim, In Ho
    • Animal Bioscience
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    • v.35 no.8
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    • pp.1215-1222
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    • 2022
  • Objective: A feeding trial was carried out to determine the effect of dietary inclusion of silymarin seed extract on growth performance, nutrient digestibility, excreta microbiota, excreta gas emission, blood profiles, and meat quality in broilers. Methods: A total of 1,088 one-day-old Ross 308 broiler chicks (mixed-sex) with an initial body weight of 42.34±0.82 g, were randomly allocated into 1 of 4 dietary treatments with 17 replicates of 16 chicks per cage and fed a basal diet supplemented with 0%, 0.02%, 0.04%, and 0.06% of silymarin. Results: The inclusion of silymarin supplementation linearly increased the body weight of broilers during days 7 to 21 and 1 to 35 days. On day 35, broilers fed a diet containing graded levels of silymarin supplementation linearly increased the nutrient digestibility of dry matter, gross energy, and nitrogen and cecal Lactobacillus counts (p = 0.038). While silymarin supplement linearly reduced the methyl mercaptans (p = 0.039) and acetic acid (p = 0.007) emission in broilers. No significant effects were observed on the blood profile. Relative weights of organs such as breast muscle, bursa of fabricius were increased (linear effect, p<0.05), water holding capacity was enhanced by increasing the silymarin level from 0% to 0.06%. A linear reduction (p>0.05) in drip loss from meat samples during days 1, 3, 5, and 7 by the addition of graded level of silymarin to the diet. Conclusion: An increasing level of silymarin supplementation to the diet of broiler would be beneficial to enhance growth performance, nutrient digestibility, excreta microflora, blood profile, and meat quality traits.

Expression Patterns of SQS in Different Tissues in Amaranth Grains (Amaranthus cruentus L.)

  • Young-Jun Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.294-294
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    • 2022
  • To date, there have been no reports on the cloning and characterization of a gene encoding SQS from Amaranthus, although there have been some reports on methods of extracting and purifying squalene from Amaranthus seeds. In this study, we monitored the expression pattern of the amaranth SQS gene in seeds at different developmental stages and in different tissues. The transcript expression pattern of the SQS gene was investigated using total RNA isolated from seeds at different stages of development. There were low levels of SQS transcripts at the early stage of seed development, and the levels remained low until the middle developmental stage. The expression of SQS increased rapidly to reach a peak at the mid-late developmental stage, and then declined dramatically. This pattern of expression was consistent with the results of RT-PCR analyses. All RNA samples generated a fragment of the expected size (183-bp). The amaranth SQS was expressed at low levels during the initial to middle stages of seed development, and its expression level increased at the mid-late development stage. Also The tissue-specific expression of amaranth SQS was determined by quantifying its mRNA in total RNA isolated from the leaves, petioles, stems, and roots of seedlings at the four- and six-leaf stages. Using qRT-PCR and RT-PCR analysis, we detected amaranth SQS transcripts in some of the tissues at the six-leaf stage, but in none of the tissues from plants at the four-leaf stage. SQS transcripts accumulated in almost equal amounts in stems and roots, while a lower level accumulated in leaves and petioles during seedling development at the four- to six-leaf stages. This study provides useful information about the molecular characterization of the SQS clone isolated from grain amaranth. A basic understanding of these characteristics will contribute to further studies on the amaranth SQS.

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