• 대한한방부인과학회지
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• 제18권3호
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• pp.77-91
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• 2005

Purpose : Trichosanthis Radix is traditional medical herb which has been shown to inhibit tumor cell proliferation. In this study, the effects of Trichosanthis Radix extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Human uterine cervical carcinoma cells line, ME-180, was used for the study. The cells were treated with varying concentrations of Trichosanthis Radix extract. Cell growth and inhibitory rate were measured by MTT assay. Apoptosis induction was detected by fluorescence microscopy, DNA ladder formation and flow cytometry. Results : Trichosanthis Radix extract inhibited the growth of human uterine cervical carcinoma cells in a dose-dependent manner. It induced ME-180 cells to undergo apoptosis including fragmented nuclei and nucleosome-sized DNA fragmentation. Flow cytometric analysis showed the increasing rate of apoptotic cells by Trichosanthis Radix extract. Reduction of mitochondrial membrane potential and increase in caspase-3 activity and were found in ME-180 cells treated with Trichosanthis Radix extract. Conclusion : Our data suggest that Trichosanthis Radix extract inhibit the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity via caspase-3 activation initiated by depolarization of mitochondria.

• 한국환경과학회지
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• 제8권5호
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• pp.569-574
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• 1999

This study was carried out to determine the antimutagenic effects of genistein on the somatic mutagenicity induced by aflatoxin B1 (${AFB}_1$), using Drosophila wing spot test system. Mutagen alone or mutagen with genistein were administered to the heterozygous(mwh/+) third instar larvae by feeding, and somatic cell mutations were detected in adult fly wing hairs. Genistein did not show any mutagenicity with the feeding concentrations of 5~15% in the test system. As the feeding concentrations of genistein increased, genistein inhibited the mutagenicity induced by AFB1 (14.6%~62.2% inhibition rate), while as the concentrations of AFB1 increased, small much spots that arise mostly from chromosome deletion and nondisjunction were more strongly suppressed by genistein than the large mwh spots from chromosomal recombination. In each group of different AFB1 concentrations, the rate of inhibition for total mwh spots was dependent on the dose of genistein. These results indicate that genistein have inhibitory effect on the mutagenicity induced by a mtagen, ${AFB}_1$. It seems to suggest that genistein may exert inhibitory effects to mutagenic and/or carcinogenic properties of DNA damaging agents.

• 한국지하수토양환경학회지:지하수토양환경
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• 제26권5호
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• pp.1-8
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• 2021

A series of pot experiments were conducted to quantitatively estimate inhibitory effects of reclaimed soil on the growth of Lentil (Lens culinaris) with two soils remediated by land farming (DDC) and low temperature thermal desorption(YJ), respectively. After cultivation in a growth chamber for 8 days, plants were harvested for the analysis of 8 indices including chlorophyll-a and carotenoid in leaves, shoot fresh weight, root dry weight, root length, number of later roots, specific root length (SRL) as well as germination rate in comparison to control experiment conducted on nursery soil. Root length was estimated by SmartRoot program from the digital images of the roots. The results showed germination rate on YJ and DDC soil decreased 29 and 71%, respectively. In comparison to the control, the averaged value of the 8 indices for YJ and DDC soil showed overall growth inhibition was 48 and 68%, respectively. When the same experiment was conducted with 25% (W/W) vermiculate amended soil, plant growth on each soil was comparable to that of the control. The results implies reclaimed soils requires additional processes and/or amendments to reuse for plant growth.

• BMB Reports
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• 제32권3호
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• pp.226-231
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• 1999

In vitro effects of acetone on cytochrome P450 (P450)-dependent benzo(a)pyrene (B(a)P) hydroxylation supported by cumene hydroperoxide (CuOOH) or NADPH/$O_2 $ systems were studied using 3-methylcholanthrene-pretreated rat liver microsomes. The maximal rate of B(a)P hydroxylation at constant concentration ($80\;{\mu}M)$ of the substrate was observed in the presence of $30\;{\mu}M$ CuOOH. However, at concentrations higher than $30\;{\mu}M$ CuOOH the hydroxylation rates were rapidly decreased. In contrast to CuOOH, at a concentration of $200\;{\mu}M$ NADPH, B(a)P hydroxylation rate reached a plateau. At concentrations higher than $200\;{\mu}M$ NADPH, the rates of substrate hydroxylation were maintained at the maximal rate with no inhibition. Acetone at 1% (v/v) enhanced both CuOOH- and NADPH/$O_2$-supported B(a)P hydroxylation at the optimal concentrations of the cofactors. At concentrations higher than 1% (v/v) acetone, substrate hydroxylation was sterero specific under the support of these two cofactors; it was strongly enhanced with $30\;{\mu}M$ CuOOH, but rather inhibited in the $200\;{\mu}M$> NADPH/$0_2 $ system. The lipid peroxidation rate induced during CuOOH-supported P450-dependent B(a)P hydroxylation was increased as CuOOH concentrations were increased. Acetone in the concentration range of 2.5~7.5%(v/v) inhibited lipid peroxidation during CuOOH supported B(a)P hydroxylation. The finding that CuOOH-supported B(a)P hydroxylation is greatly enhanced by acetone suggests that acetone may contribute more to the activation of oxygen (for the insertion of oxygen into the substrate) in the presence of CuOOH than with NADPH/$O_2$. Acetone may also contribute to the partial inhibition of destruction of microsomal membranes by lipid peroxidation.

• 유기물자원화
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• 제15권4호
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• pp.131-137
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• 2007

황산염 환원조건에서의 리그노셀룰로오스의 분해에 대하여 고찰하였다. 특히, 리그닌에 대한 셀룰로오스의 비(C/L 비)를 각각 42.15, 4.59, 2.51, 1.14, 0.7로 하여 리그닌과 셀룰로오스의 상호작용에 대하여 고찰하였다. 셀룰로오스의 분해율은 1차 반응식에 의해 계산되어져, C/L 비가 감소할수록 반응상수는 감소하여 셀룰로오스의 분해에 대한 리그닌의 저해작용을 보여 주였다. 1차 반응식에 의한 반응상수와 리그닌의 함량의 증가에 대한 셀룰로오스의 분해율은 0.97의 $R^2$ 값을 가지며 로그힘수에 의해 표현할 수 있었다. 리그노셀룰로오스의 분해율 또한 C/L 비와 로그함수의 관계를 가지며 리그닌의 함량이 많을수록 감소하였다. 리그닌의 분해율은 C/L 비가 2.51 및 1.14인 조건에서 4.59 및 0.7의 조건보다 높게 나타나, 공동기질로서의 과도한 탄소원은 리그닌분해에 장애가 됨을 보여 주였다.

• KSBB Journal
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• 제10권3호
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• pp.323-334
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• 1995

Hybridoma 세포의 세포 성장 속도, monoclo nal antibody의 생산성 및 세포 대사에 미치는 glucose의 영향이 조사되었다. IgG2,를 생산하는 m mouse-mouse hybridona VIII H-8 세포가 모델로 이용되었다. Glucose 농도에 따른 바성장속도의 변화는 기질저해형식 (substrate inhibition type) 의 성장모델로 나타낼 수 있었다. 초기 glucose 농도 4g/f까지는 최대 세포밀도의 증가를 보여 주었다. Glucose는 세포사망속도에 큰 영향을 나타내었고 glucose 농도와 비사망속도 간에는 반비례의 관계가 성립됨을 보였다. Glucose 농도가 증가될수록 세포의 생존율과 monoclonal an tibody의 생산이 증가되었다. Glucose 놓도가 증가될수록 glucose의 비소비속도가 증가되 었고, 초기 glucose 농도의 증가는 lactate의 총괄 비 생산속도의 증대를 가져왔다. 암모늄 이온의 총괄비 생산속도는 초기 Glucose의 농도에 의존하 였지만 암모늄 이온의 전반적인 생성속도는 초기 glucose 농도에 거 의 무관함을 보여 주었다.

• The Korean Journal of Physiology and Pharmacology
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• 제5권5호
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• pp.423-431
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• 2001

Some recent investigations revealed that vasodilatory action of adenosine is mainly not mediated by surface A2 receptor and suggested the existence of an intracellular action site. In the present study, we tried to differentiate intracellular from extracellular site of adenosine action in the regulation of coronary flow. In perfused rabbit hearts, concentration-response curve of coronary flow to exogenous adenosine was constructed in the presence or absence of dipyridamole, an inhibitor of transmembrane purine transport. Inhibition of cellular adenosine uptake by dipyridamole suppressed the increase of flow rate while enhancing the decrease in heart rate induced by exogenous adenosine. In another series of experiments, perfused rabbit hearts were subjected to energy deprivation in order to increase the production of endogenous adenosine. Energy deprivation along with dipyridamole administration resulted in higher coronary flow rate. Lower perfusate adenosine concentration was observed along with higher tissue adenosine content in this group. These results implied that coronary flow rate is determined not by interstitial adenosine concentration but by intracellular activity of adenosine. To confirm the effects of dypiridamole in vivo, direct measurement of interstitial adenosine concentration by mycrodialysis along with the assay of intracellular adenosine content was performed after intranenous dipyridamole administration. After dipyridamole infusion, intracellular adenosine content was markedly increased while interstitial adenosine concentration was not altered. In another series of experiments, the right shift of concentration-response curve of adenosine-induced vasodilation by 8-phenyltheophilline, a representative adenosine receptor antagonist, was mostly abolished by prior administration of prazosin, indicating that the influence of 8-PT on the adenosine action is not attributed to the inhibition of A2 receptor but related to the suppression of ${\alpha}-adrenoceptor$ activation. From these results, we concluded that adenosine acts intracellularly to regulate the coronary blood flow.

• 한국작물학회지
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• 제29권3호
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• pp.285-290
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• 1984

대두에 왜화제인 B 995와 CCC를 살포시기별(본엽 4, 5, 6 매), 농도별 (500, 1000, 2000, 3000 ppm)로 엽면살포하여 생육 및 수량에 미치는 영향을 구명하고자 본 실험을 수행하여 얻어진 주요결과는 다음과 같다. 1. 약제별로는 경장에서만, 살포시기별로는 결협율에만, 그리고 농도별로는 경장, 분지수, 결협율, 주당협수와 수량에서만 유의성이 인정되었다. 2. 견장의 단축은 CCC보다는 B 995가 더 효과적이었으며 결협율은 본엽4 매시의 살포에서 가장 높았다. 3.농도별효과를 보면 저농도보다는 고농도인 3,000ppm에서 경장의 단축, 분지수, 결협율 그리고 주당협수의 증가와 수량의 증대를 나타냈다. 4. 주경절수, 경건중, 엽록소와 100입중은 약제살포시기 및 농도별로 유의성이 없었다. 5. 수량은 경장과는 부의 상관이었고 분지수, 주당협수, 결협율과는 정의 상관이었다. 6. 왜화제의 엽면살포는 주요수량구성 요소증대에 상당히 효과적이었다.

• 디지털융복합연구
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• 제10권11호
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• pp.559-564
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• 2012

생약제제 중에서 귤피는 항산화작용, 순환기계 질병의 예방, 항염증, 항알레르기, 항균, 항바이러스, 지질 저하 작용, 면역 증강작용, 모세혈관 강화작용 등이 있는 것으로 보고되고 있다. 본 연구에서는 귤피 추출물이 치아우식 원인균으로 알려진 S. mutans에 미치는 항균효과와 GTase활성에 미치는 영향에 대해 알아보고자 하였다. 추출물을 5, 10, 20 mg/ml의 농도로 배지에 첨가한 후 S. mutans의 성장억제효과를 확인한 결과, 농도가 높을수록 colony의 수가 현저히 줄어드는 것을 관찰 할 수 있었다. 8 시간 후 측정하였을때 5 mg/ml에서 92%, 10 mg/ml에서 95%, 20 mg/ml 에서 99%의 높은 성장억제율을 나타내었다. 또한 추출물의 GTase 활성 저해율을 측정한 결과, 추출물의 농도가 높아질수록 저해율이 급격히 증가하는 것으로 나타났으며, 5 mg/ml에서는 42%의 강한 활성 저해 효과를 보였다.

• 동의신경정신과학회지
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• 제20권3호
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• pp.49-64
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• 2009

Objectives : The water and methanol extracts of Aconitum carmichaeli(Aconiti Tuber Preparat) were investigated for their anti-depressant effects. Methods : In this study, reserpine-induced hypothermia test, tail suspension test and hot plate test. Additionally, the brain monoamine oxidase activity was determined in vivo. Results: In the reserpine-induced hypothermia test, both extracts suppressed the fall of body temperature compared to the control group in a dose-dependent manner, suggesting the inhibition on hypothermia. In the tail suspension test, the methanol extract dose-dependently reduced the duration of immobility by 28.4% at a dose of 1 g/kg compared to control group, which is more effective than the water extract. In the hot plate test, the water extract and methanol extract increased the jump latency time compared to the control group, showing the inhibition rate of 198% and 182%, respectively, at a dose of 1 g/kg. Methanol extracts potently inhibited the brain monoamine oxidase activity in an in vivo assay compared to the control group, showing 84.6% inhibition, but the water extract revealed very weak activity. Conclusions : Above results suggested that the extract of Aconitum carmichaeli can be useful for the prevention and treatment of depression.