• Title/Summary/Keyword: inhibition characteristics

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Hydrolysis Characteristics of Goat Milk $\beta-Casein$ by Enzyme and Angiotensin Converting Enzyme Inhibition Effects of Hydrolysate (산야유 $\beta-Casein$의 효소 가수분해 특성과 가수분해물의 Angiotensin Converting Enzyme 저해 효과)

  • Park Yong-Kuk;Kwon Il-Kyoung;Kim Gur-Yoo
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.238-243
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    • 2005
  • This study was carried out to understand hyrolytic characteristics of $\beta-casein$ by enzyme in goat milk and to measure the inhibition effect of the ACE of the hydrolysate. In order to conduct the experiment, $\beta-casein$ of goat milk was separated using Mono S HR 5/5, a cation exchange column. The separated $\beta-casein$ was treated with trypsin of animal hydrolysis enzymes, in an effort to verify the characteristics of hydrolysis. The inhibition activity of ACE was measured and the results are as follows. By analyzing the hydrolysate separated from the trypsin-processed $\beta-casein$ of goat milk, the inhibition effect of the ACE was measured trypsin-hydrolyzed $\beta-casein$ demonstrated a $25.36\pm0.79\%$ of inhibition effect and the $IC_{50}$ of the hydrolysate from the trypsin-processed $\beta-casein$ reached $308.7\pm2.77({\mu}g/mL)$.

Growth Characteristics of Ultrahigh-density Microalgal Cultures

  • Richmond, Amos
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.6
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    • pp.349-353
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    • 2003
  • The physiological characteristics of cultures of very high cell mass (e.g. 10g cell mass/L), termed“ultrahigh cell density cultures”is reviewed. A close relationship was found between the length of the optical path (OP) in flat-plate reactors and the optimal cell density of the culture as well as its areal (g m$\^$-2/ day$\^$-1/) productivity. Cell-growth inhibition (GI) unfolds as culture density surpasses a certain threshold. If it is constantly relieved, a 1.0cm OP reactor could produce ca. 50% more than reactors with longer OP, e.g. 5 or 10cm. This unique effect, discovered by Hu et al. [3], is explained in terms of the relationships between the frequency of the light-dark cycle (L-D cycle), cells undergo in their travel between the light and dark volumes in the reactor, and the turnover time of the photosynthetic center (PC). In long OP reactors (5cm and above) the L-D cycle time may be orders of magnitude longer than the PC turnover time, resulting in a light regime in which the cells are exposed along the L-D cycle, to long, wasteful dark periods. In contrast, in reactors with an OP of ca. 1.0 cm, the L-D cycle frequency approaches the PC turnover time resulting in a significant reduction of the wasteful dark exposure time, thereby inducing a surge in photosynthetic efficiency. Presently, the major difficulty in mass cultivation of ultrahigh-density culture (UHDC) concerns cell growth inhibition in the culture, the exact nature of which is awaiting detailed investigation.

Chemical Responses and Fitness-Related Characteristics of Dichlofluanid-Resistant and -Sensitive Botrytis cinerea Isolates (Dichlofluanid 저항성 및 감수성 잿빛곰팡이병균(Botryits cinerea)의 약제 반응과 생태 적응력과 관련된 특성)

  • 임태헌;김병섭;조광연;차병진
    • Korean Journal Plant Pathology
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    • v.11 no.3
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    • pp.245-251
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    • 1995
  • Nine hundred and ninety-two isolates of Botrytis cinerea were obtained from infected strawberries, tomatoes and cucumbers in Taejon, Gongju, Puyo, Nonsan and Kimhae in Korea. Six hundred forty-two (64.7%) isolates were benomyl resistant (BR), 245 (24.7%) were procymidone resistant (PR), and 105 (10.6%) were dichlofluanid resistant (DR). In the minimum inhibitory concentration (MIC) test, DR isolates showed mycelial growth on the PDA incorporated with 100 or 500 $\mu\textrm{g}$/ml of dichlofluanid, while dichlofluanid sensitive (DS) isolates did not grow on the PDA incorporated even with 10 $\mu\textrm{g}$/ml of dichlofluanid. Chemical concentrations for inhibition of spore germination were much lower than those for inhibition of mycelial growth. IC50 values, the effective concentrations for 50% inhibition of spore germination, for DR were 0.11~0.29 $\mu\textrm{g}$/ml, whereas they were 0.04~0.09 $\mu\textrm{g}$/ml for DS isolates. In comparison of fitness-related characteristics such as virulence, sclerotial formation, and sporulation, DR isolates were inferior to DS isolates. However, mycelial growth was little different between DR isolates and DS isolates.

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Characteristics of the Bacteriocin Produced from Streptococcus salivarius subsp. thermophilus (Streptococcus salivarius subsp. thermophilus가 생산하는 Bacteriocin의 특성)

  • 이장혁;장효일
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.7-12
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    • 1994
  • One bacterial strain, that had made the largest inhibition zone at the antagonism assay and also that lost the inhibition activity by the protease treatment, was isolated from raw milk. That strain was identified as Streptococcus salivarius subsp. thermophilus. The specific growht rate of this strain was maximum at 45$\circ $C. However, at this temperature the strain produced no bacteriocin. The bacteriocin activity was quite stable even at high temperature. Moreover, the activity of the vacteriocin was sensitive to proteases. but not to $\alpha $-amylase, DNase I, or RNase.

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Inhibition of Monamine Oxidase by a Flavone and Its Glycoside from Ixeris dentata Nakai

  • Chung, Ha-Sook
    • Preventive Nutrition and Food Science
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    • v.8 no.2
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    • pp.141-144
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    • 2003
  • Ixeris dentata Nakai (Compositae) is a perennial herb which has been used as a folk medicine for treating diabetes and gastroenteric troubles in Korea. Active compounds were isolated from the aerial parts of Ixeris dentata through the bioassay-guided fractionation and isolation method evaluated for inhibition of monoamine oxidase (MAO) in vitro. The compounds were identified as 5,7,3',4'-tetrahydroxyflavone (1) and 5,7,3',4'- tetrahydroxyflavone 7-glucoside (2), based on physical and spectroscopic characteristics. Compounds 1 and 2 showed a selective inhibition of type B MAO (MAO-B) activity, with IC/sub 50/ values of 15.3 μM and 36.4 μM, respectively, but did not inhibit type A MAO (MAO-A) activity.

Corrosion Inhibition Screening of 2-((6-aminopyridin-2-yl)imino)indolin-3-one: Weight Loss, Morphology, and DFT Investigations

  • Nadia Betti;Ahmed A. Al-Amiery
    • Corrosion Science and Technology
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    • v.22 no.1
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    • pp.10-20
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    • 2023
  • Because of its inexpensive cost, mild steel is frequently employed as a construction material in different industries. Unfortunately, because of its limited resistance to corrosion, a protective layer must be applied to keep it from decaying in acidic or basic environments. The presence of heteroatoms, such as nitrogen, oxygen, and pi-electrons in the Schiff base could cause effective adsorption on the mild steel surface, preventing corrosion. The weight loss method and scanning electron microscopy (SEM) were used to investigate the inhibitory effects of APIDO on mild steel in a 1 M hydrochloric acid environment. The efficiency of inhibition increased as the inhibitor concentration increased and decreased as the temperature increased. The SEM analysis confirmed that the corrosion inhibition of APIDO proceeded by the formation of an organic protective layer over the mild steel surface by the adsorption process. Simulations based on the density functional theory are used to associate inhibitory efficacy with basic molecular characteristics. The findings acquired were compatible with the experimental information provided in the research.

Study of Inhibition Characteristics of Slurry Additives in Copper CMP using Force Spectroscopy

  • Lee, Hyo-Sang;Philipossian Ara;Babu Suryadevara V.;Patri Udaya B.;Hong, Young-Ki;Economikos Laertis;Goldstein Michael
    • Transactions on Electrical and Electronic Materials
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    • v.8 no.1
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    • pp.5-10
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    • 2007
  • Using a reference slurry, ammonium dodecyl sulfate (ADS), an anionic and environmentally friendly surfactant, was investigated as an alternative to BTA for its inhibition and lubrication characteristics. Results demonstrated that the inhibition efficiency of ADS was superior to that of BTA. Coefficient of friction (COF) was the lowest when the slurry contained ADS. This suggested that adsorbed ADS on the surface provided lubricating action thereby reducing the wear between the contacting surfaces. Temperature results were consistent with the COF and removal rate data. ADS showed the lowest temperature rise again confirming the softening effect of the adsorbed surfactant layer and less energy dissipation due to friction. Spectral analysis of shear force showed that increasing the pad-wafer sliding velocity at constant wafer pressure shifted the high frequency spectral peaks to lower frequencies while increasing the variance of the frictional force. Addition of ADS reduced the fluctuating component of the shear force and the extent of the pre-existing stick-slip phenomena caused by the kinematics of the process and collision event between pad asperities with the wafer. By contrast, in the case of BTA, there were no such observed benefits but instead undesirable effects were seen at some polishing conditions. This work underscored the importance of real-time force spectroscopy in elucidating the adsorption, lubrication and inhibition of additives in slurries in CMP.

Adsorption Characteristics of Trihalomethanes in a Bi-solute System (이용질계(二溶質系)에서 Trihalomethane 의 흡착특성(吸着特性))

  • Chung, Tai Hak;Ahn, Kwang Seog
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.7 no.2
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    • pp.37-44
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    • 1987
  • Adsorption characteristics of chloroform, carbon tetrachloride, and crystal violet were investigated in single-and bi-solute systems. Single-solute adsorption for each solute was well described by Freundlich equation. Severe inhibition was recorded in bi-solute adsorption systems despite of low solute concentration of less than 1 mg/l. Inhibition of chloroform adsorption by carbon tetrachloride, similar compound in chemical structure, was much higher than by crystal violet of which chemical structure is quite different. Highest inhibition was observed at crystal violet adsorption by chloroform. While, inhibition caused by each other was almost equal between chloroform and carbon tetrachloride. Bi-solute adsorption was well described by Friz-Schl${\ddot{u}}$nder model and by much simpler 3 parameter Freundlich equation.

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Characteristics of Organic Acid Degradation by Yeast (고농도 유기산폐수의 효모에 의한 분해연구)

  • 김석원;허병기;김은기
    • KSBB Journal
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    • v.14 no.2
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    • pp.136-140
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    • 1999
  • Characteristics of organic acid degradation by isolated yeast strain was investigated. Optimum initial pH was 5. Increase in cell mass was proportional to the decrease in organic acid degradation. Also no accumulation of byproduct was observed during degradation. Acetic acid degraded fast, followed by butyric acid and propionic acid in order. No significant substrate inhibition was observed up to 12 g/L of acetic acid 7 g/L of propionic acid, respectively. However, inhibition of butyric acid was significant above 4 g/L. Cell mass yield was 0.2-0.4 g cell/g acids and decreased at high decreased at high organic acid concentration. 95% of organic acid (7.5 g/L), corresponding to 13,000 ppm, was degraded in 30-40 hours.

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Effects of Plant Growth Regulator Treatment on Ginseng berry and seed development in Panax ginseng C. A. Meyer (식물생장조절제 처리가 인삼의 장과 및 종자 형성에 미치는 영향)

  • Jo, Seo-Ri;Kim, Jung-Sun;Lee, Nu-Ri;Choi, Jae-Eul
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.5
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    • pp.315-319
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    • 2012
  • This study was conducted to select plant growth regulators effective at ginseng berry set inhibition to help root growth in Korean ginseng (Panax ginseng C. A. Meyer). PGRs (ethephon, gibberellic acid, maleic hydrazide, coumarin) were applied to field grown 5-year-old Korean ginseng between one and two times, before and during bloom in 2009, 2010. The number of treatment was more effective in ginseng berry set inhibition when used two times compared with one time in GA 1,000 ppm, MH (5,000, 10,000 ppm), coumarin (5,000, 10,000 ppm) treatment. According to treatment period of plant growth regulator, ginseng berry set inhibition rate from 20days before flowering date to 5days after blooming was the highest in MH 5000 ppm showing 99.9% and the lowest in GA 100 ppm showing 32.8%. The spray treatments of Ethephon (50, 150 ppm) and MH (5,000, 10,000 ppm) from 20 days before the flowering bloom up to 5 days before, and coumarin (5,000, 10,000 ppm) from 20 days to 6 days and before blooming that induced the inhibitory effect more than 90% after 12 weeks. Considering ginseng berry set inhibition characteristics and treatment period ethephon and coumarin was important about applied period but, MH treatment appeared to effective ginseng berry set inhibition regardless of treatment period.