• Reproductive and Developmental Biology
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• v.29 no.3
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• pp.181-186
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• 2005

This experiment was conducted to investigate effects of the two different in vitro production systems, serumcontaining system (IVM, IVF and IVC; TCM199, TALP and CR1aa) and serum-free system (IVM, IVF and IVC; IVMD101, IVMD100 and IVMD101), on the development of in vitro fertilized or DNA-microiniected embryos. We also examined the effect of DNA dosage and its expression pattern in embryos. The DNA used for microinjection was a green fluorescence protein gene. The development rates to $\geq$ 2cell, 8cell and blastocyst stage were significantly higher in vitro fertilized embryos than those in DNA-microinjected embryos. The development rate to the 8-cell stage was significantly higher in serum-free system than in serum-containing system (p<0.05; $3.3\%\;vs.\;15.5\%\;and\;21.4\%$, respectively). The development rates to the blastocyst stage of in vitro fertilzed or DNA-microinjected embryos between two different culture system ($2.7\%\;vs.\;2.3\%\;and\;23.0\%\;vs.\;23.6\%$, respectively) were not different. The development rates of embryos injected 2 ng/uL DNA was higher. than those of embryos injected 4 or 8 ng/uL DNA. The GFP expression rate of 1-cell embryos was significantly higher than that of 2-cell and 4-cell embryos, whereas the rates were not different between 4-cell and blastocyst-stage embryos.

• Archives of design research
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• v.16 no.2
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• pp.385-394
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• 2003

Modem market's tendency is displaying brand strategy of Corporation sells product and consumer buys rand'.Brand acts by intermediate that reflect consumer's request and experience and attach product and consumer hat produce. Successful brand intended strategy empirical esthetics enemy, and consumer recognizes brand to novel experience and think by one fixed idea (awareness). any corporations wish to get maximum effect into minimum investment expense that reduce huge public elations and advertisement charge. Do to incuse impression for product brand through direct interview with consumer at purchase visual point, and do function hat drive purchase and the importance great rawly. Therefore, sight expression of brand image must be able to from immobile one impression in consumer's spirit being inked nearly with brand .Brand that situate by affirmative impression once can arrive in purchase by brand without alternative information being withdrawn by consumer's emory. Solution of visual expression for this being city cornification laying stress on logo and mark of brand as central element of brand image, speak as communication actor who back this. This to be Dija as direction that product and connection are deep before meaning that is sight language enemy who allow fetters special quality and the seniority in age sex language enemy of brand as ell as have direction of by methods method for problem solution present can .Therefore, relation with brand image analysis of sports equipment and consumer product purchase that this research forms market economy theoretical investigating to be effective and present direction that is image-making to be consistency this the purpose be.

• Journal of Korea Technology Innovation Society
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• v.10 no.3
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• pp.486-508
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• 2007

This study identifies key determinants of participation in UCC services. Incorporating insights from the flow theory, we examine the effects of psychological factors of social presence, self expression, arousal, and challenge as well as web-site characteristics variables of media easiness, contents usability, and immediateness. We have done a sample survey of internet users and collected 260 responses. Using Windows SPSS/PC 12.0 Package, we have performed statistical analyses including a correlation analysis, a factor analysis, and a multiple regression analysis. The result of the study is as follows. Psychological variables of perceived social presence, self expression, arousal, and challenge all show positive significant effect on participation in UCC service. Among web site characteristics, media easiness, which consists of a web structure that is easy-to-use, user friendliness, and personalized service, demonstrates a positive significant effect on participation in UCC services. Immediateness also has a positive significant effect. Some of the practical implications of the result are follows. We should improve user access to platforms of UCC service by opening up platforms. This will heighten perceived challenge which has the strongest influence on participation in UCC services. We need to focus on multimedia services and adjust to the cultural code of netizen who crave for visual expressions and on the spot on-line activities. Also suggested is that contributions made by participants need to be acknowledged through such provisions as profit sharing. Needs for individualized service, which is an aspect of media easiness, should also be addressed. Participants tend to value individuality while at the same time accepting broader trends. Information services need to be customized for individuals. In UCC centered internet businesses, netizen consumers are presumer. They are consumers and producers at the same time, and consumer needs should also be explored for the success of internet businesses.

• Korean Journal of Cognitive Science
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• v.12 no.1_2
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• pp.77-89
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• 2001

Applying perceptual hierarchy of facial feature points, a neural network model for recognizing facial expressions was designed. Input data were convolution values of 150 facial expression pictures by Gabor-filters of 5 different sizes and 8 different orientations for each of 39 mesh points defined by MPEG-4 SNHC (Synthetic/Natural Hybrid Coding). A set of multiple regression analyses was performed with the rating value of the affective states for each facial expression and the Gabor-filtered values of 39 feature points. The results show that the pleasure-displeasure dimension of affective states is mainly related to the feature points around the mouth and the eyebrows, while a arousal-sleep dimension is closely related to the feature points around eyes. For the filter sizes. the affective states were found to be mostly related to the low spatial frequency. and for the filter orientations. the oblique orientations. An optimized neural network model was designed on the basis of these results by reducing original 1560(39x5x8) input elements to 400(25x2x8) The optimized model could predict human affective rating values. up to the correlation value of 0.886 for the pleasure-displeasure, and 0.631 for the arousal-sleep. Mapping the results of the optimized model to the six basic emotional categories (happy, sad, fear, angry, surprised, disgusted) fit 74% of human responses. Results of this study imply that, using human principles of recognizing facial expressions, a system for recognizing facial expressions can be optimized even with a a relatively little amount of information.

• The KIPS Transactions:PartD
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• v.14D no.6
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• pp.585-596
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• 2007

XML data generally consists of a hierarchical tree-structure which is reflected in mechanisms to store and retrieve XML data. Therefore, when storing XML data in the database, the hierarchical relationships among the XML elements are taken into consideration during the restructuring and storing of the XML data. Also, in order to support the search queries from the user, a mechanism is needed to compute the hierarchical relationship between the element structures specified by the query. The structural join operation is one solution to this problem, and is an efficient computation method for hierarchical relationships in an in database based on the node numbering scheme. However, in order to process a tree structured XML query which contains a complex nested hierarchical relationship it still needs to carry out multiple structural joins and results in another problem of having a high query execution cost. Therefore, in this paper we provide a preprocessing mechanism for effectively reducing the cost of multiple nested structural joins by applying the concept of equivalence classes and suggest a query path reduction algorithm to shorten the path query which consists of a regular expression. The mechanism is especially devised to reduce path queries containing branch nodes. The experimental results show that the proposed algorithm can reduce the time requited for processing the path queries to 1/3 of the original execution time.

• Korean Journal of Health Education and Promotion
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• v.22 no.4
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• pp.73-90
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• 2005

Objectives: Smokers who had failed to quit smoking were frequently reported that life stress mostly interrupted their abstention. Stress vulnerability model for smoking cessation has been considered, and most of contemporary smoking cessation programs help smokers develop coping strategies for stressful situations. This study aims to investigate the appropriate coping styles for stress of abstention from smoking. The result of investigating the relationship between abstention following smoking cessation program and coping styles would suggest useful information for those who want to stop smoking and health practitioners who help them. Methods: Participants were 69 smokers (62 males, 7 females) participated in a hospitalized smoking cessation program, whose mean age was 44.89 (SD=9.61). Participants took medical test and completed questionnaires and psychological tests including: Fagerstrom Test for Nicotine Dependence and Multidimensional Coping Scale. To identify participants' abstention, researchers followed them for 2 years. To identify whether abstained or not and encourage them to abstain, researchers called them on the telephone once a week for 3 months. After 3 months, they were contacted every other week till 6 months passed since they left smoking cessation program. And they were contacted once a month for other 18months. Researchers also contacted their family to identify their abstention. Data Mining Decision Tree was performed with 37 variables (13 variables for the coping styles and 24 smoking-related variables) by Answer Tree 3.0v Results: Forty four (63.8%) out of sixty nine for 2 weeks, 34 (49.3%) for 6 months, 25 (36.2%) abstained for 1 year, and 22 (31.9%) abstained for 2 years. Participants of this study abstained average of 286.77 days from smoking. Included variables of a Decision Tree model for this study were positive interpretation, emotional expression, self-criticism, restraint and emotional social support seeking. Decision Tree model showed that those (n=9) who did not interpret positively (<=7.5) and criticized themselves (>6.5) abstained 23 days only, while those (n=9) who interpreted positively (>7.5), expressed their emotion freely (>6.5), and sought social support actively (>11.5) abstained 730 days, till last day of the investigation. Conclusion: The results of this study showed that certain coping styles such as positive interpretation, emotional expression, self-criticism, restraint and emotional social support seeking were important factors for long-term abstention from smoking. These findings reiterate the role of stress for abstention from smoking and suggest a model of coping styles for successful abstention from smoking. Despite of limitation of this study, it might help smokers who want to stop smoking and health practitioners who help them.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.46 no.1
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• pp.112-120
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• 2009

Face membership authentication is to decide whether an incoming person is an enrolled member or not using face recognition, and basically belongs to two-class classification where support vector machine (SVM) has been successfully applied. The previous SVMs used for face membership authentication have been trained and tested using image feature vectors extracted from member face images of each class (enrolled class and unenrolled class). The SVM so trained using image feature vectors extracted from members in the training set may not achieve robust performance in the testing environments where configuration and size of each class can change dynamically due to member's joining or withdrawal as well as where testing face images have different illumination, pose, or facial expression from those in the training set. In this paper, we propose an effective class discriminating feature vector-based SVM for robust face membership authentication. The adopted features for training and testing the proposed SVM are chosen so as to reflect the capability of discriminating well between the enrolled class and the unenrolled class. Thus, the proposed SVM trained by the adopted class discriminating feature vectors is less affected by the change in membership and variations in illumination, pose, and facial expression of face images. Through experiments, it is shown that the face membership authentication method based on the proposed SVM performs better than the conventional SVM-based authentication methods and is relatively robust to the change in the enrolled class configuration.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.140-140
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• 2017

Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

• Science of Emotion and Sensibility
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• v.16 no.1
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• pp.95-106
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• 2013

The study compares accuracies between humans and computer algorithms in the discrimination of spontaneous smiles from posed smiles. For this purpose, subjects performed two tasks, one was judgment with single pictures and the other was judgment with pair comparison. At the task of judgment with single pictures, in which pictures of smiling facial expression were presented one by one, subjects were required to judge whether smiles in the pictures were spontaneous or posed. In the task for judgment with pair comparison, in which two kinds of smiles from one person were presented simultaneously, subjects were to select spontaneous smile. To calculate the discrimination algorithm accuracy, 8 kinds of facial features were used. To calculate the discriminant function, stepwise linear discriminant analysis (SLDA) was performed by using approximately 50 % of pictures, and the rest of pictures were classified by using the calculated discriminant function. In the task of single pictures, the accuracy rate of SLDA was higher than that of humans. In the analysis of accuracy on pair comparison, the accuracy rate of SLDA was also higher than that of humans. Among the 20 subjects, none of them showed the above accuracy rate of SLDA. The facial feature contributed to SLDA effectively was angle of inner eye corner, which was the degree of the openness of the eyes. According to Ekman's FACS system, this feature corresponds to AU 6. The reason why the humans had low accuracy while classifying two kinds of smiles, it appears that they didn't use the information coming from the eyes enough.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.215-222
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• 2013

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of $E^D$, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of $E^+$ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, $E^+/E^+$ (sire 1), $E^+/e$ (sire 2 and dam 3), $E^+/e$ with 4 bands of 174, 207 and 328 bp (dam 1) and $E^+/e$ with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), $E^+/E^+$ (22%) and $E^+/e$ (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, $E^+/E^+$ with 67% and $E^+/e$ with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had $E^+/e$ genotype and the dam had $E^+/E^+$ with the 3 bands or $E^+/e$ genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires ($E^+/e$) and dams ($E^+/E^+$ with the 3 bands or $E^+/e$) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.