• 한국습지학회지
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• 제15권4호
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• pp.543-553
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• 2013

우리나라에서는 유역 특성별 비점오염물질 유출특성을 파악하고 최적관리방안을 도모하기 위해 2000년 이후 다양한 형태의 실험실 규모 또는 실증 규모 최적관리방안(Best Management Practices, BMP) 시설에 대해 연구가 진행되고 있으나 유역 특성상 자정작용의 기회가 주어지지 않는 연안지역에서의 비점오염물질 유출특성 및 관리방안에 대한 연구는 미비한 상태이다. 최근 연안지역 개발에 따른 불투수층 비율은 지속적으로 증가하고 있으나, 초기우수의 직접유출을 방지하기 위한 완충 시설 및 최적관리방안 시설은 전무한 상태이다. 본 논문에서는 충남 보령시 대천항 주차장을 중심으로 연안 지역의 비점오염물질 유출특성을 파악하고, 이를 저감하기 위한 최적관리방안을 설정하여 연안지역 비점오염원 관리에 대한 기초자료를 제공하였다. 연안지역은 지역특성상 해안과 접하고 있기 때문에 지하수위가 낮고 경사차가 크지 않은 평탄한 지형을 이루고 있다. 즉, 최적관리방안을 선정하는데 있어 유출입구의 수두확보문제와 굴착 깊이에 제한을 받으며, 인공습지 및 저류지와 같은 대규모 저류형 시설의 입지조건으로는 부적합하다. 또한, 연안지역은 강우유출 특성상 염분의 함량이 높기 때문에 식물 생장에 미치는 영향이 커 식생을 이용한 자연형 최적관리방안 시설을 적용하는데도 제약을 받는다. 연안지역 비점오염원 유출 특성과 연안지역 지리 지형적 조건을 고려하여 선정된 최적관리방안 시설의 주관평가 결과 시공시 굴착 깊이가 적고, 식생의 이용이 제한적인 침투수로 형태의 시설이 적합한 것으로 사료되며 유지관리 측면에서도 유리하다.

• 생명과학회지
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• 제21권5호
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• pp.713-719
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• 2011

본 연구에서는 대두 추출물인 LES가 DNCB에 의해 인위적으로 접촉피부염을 유발시킨 랫드의 피부 회복능에 미치는 효과를 조사하였다. 알레르기 접촉피부염이 유발된 피부에 LES 17.6 mg/ml를 3-5회 도포하여 피부 표면을 조직학적 분석하여 본 결과 각질이 제거된 깨끗하고 매끄러운 상태로 되었으며, 비후화된 표피층의 두께도 점차 정상군과 같은 두께로 회복시키는 효과를 나타냈다. 또한 LES는 체내 지질함량을 낮춰 정상수준으로 회복시키는 지질함량의 개선에 효과적으로 작용하고 있으며, 혈장 내 IgE의 수준도 정상군 수준으로 감소시키고 있으며, LES에 의해 염증 반응과 관련된 iNOS와 COX-2의 활성도 감소되어 피부의 과민반응 해소에 관여하는 것으로 생각된다. LES에 포함된 주 성분 중 하나인 lunasin은 세포 침투와 함께 핵 내로 이동하는 것으로 확인되어 전체 유전자 또는 일부 유전자의 발현을 조절하는 데 관여할 것으로 추측된다. 이러한 결과로 보아 LES는 알레르기성 접촉피부염에 매우 효과적인 치료 효과를 나타내고 있기 때문에 기존의 알레르기성 접촉피부염 치료제를 대체할 수 있는 제제로서 응용이 기대된다.

• 대한약침학회지
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• 제13권4호
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• pp.5-41
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• 2010

Objectives: This study was performed to analyse four week repeated dose toxicity of Sweet Bee Venom(Sweet BV) extracted from the bee venom in Beagle dogs. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female Beagle dogs of 5-6 months old were chosen for the pilot study of four week repeated dose toxicity of Sweet BV which was administered at the level of 0.56mg/kg body weight which is eighty times higher than the clinical application dosage as the high dosage, followed by 0.28 and 0.14mg/kg as midium and low dosage, respectively. Equal amount of excipient(normal saline) to the Sweet BV experiment groups was administered as the control group every day for four weeks. Results: 1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups were appealed pain sense in the treating time compared to the control group, and hyperemia and movement disorder were observed around the area of administration in all experiment groups, and higher occurrence in the higher dosage treatment. 3. For weight measurement, Neither male nor female groups showed significant changes. 4. In the urine analysis, CBC and biochemistry didn't show any significant changes in the experiment groups compared with control group. 5. For weight measurement of organs, experiment groups didn't show any significant changes compared with control group. 6. To verify abnormalities of organs and tissues, thigh muscle which treated with Sweet BV, cerebrum, liver, lung, kidney, and spinal cords were removed and conducted histologocal observation with H-E staining. In the histologocal observation of thigh muscle, cell infiltration, inflammatory, degeneration, necrosis of muscle fiber, and fibrosis were found in both thigh tissue. And the changes were depend on the dose of Sweet BV. But another organs were not detected in any abnormalities. 7. The proper high dosage of Sweet BV for the thirteen week repeated test in Beagle dogs may be 0.28mg/kg in one time. Conclusion: Above findings suggest that Sweet BV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.

• 한국산학기술학회논문지
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• 제9권5호
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• pp.1334-1343
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• 2008

본 연구는 수계별 한정된 수자원의 효율적 관리를 위한 기존댐의 연계운영과 병행하여 댐 상·하류 유출을 고려한 종합적인 수자원관리방안 수립의 필요성이 대두됨에 따라, 고수기 및 저수기 댐 상 하류의 수계주요지점에 대한 하천유출상황을 모의할 수 있는 유출모형을 구성하는데 목적이 있다. 또한 장 단기적으로는 기존 모형을 검토하여 한국수자원공사의 "한강수계 댐 통합운영계획 수립" 업무에 활용될 수 있도록 하는데 있다. 본 연구에서는 한강수계의 소유역을 24개로 분할하였고 강우의 공간 분포를 작성하기 위해 151개의 강우관측소를 이용하여 강우자료를 정리하였다. 한강수계의 주요 제어지점으로 소양강댐, 충주댐, 충주 조정지댐, 횡성댐, 화천댐, 춘천댐, 의암댐, 청평댐, 팔당댐을 선정하였다. SSARR(Streamflow Synthesis and Reservoir Regulation) 모형을 기반모형으로 선정하여 모형의 입력자료를 작성하고 2002년의 수문자료를 이용하여 매개변수의 민감도 분석을 수행하였다. 민감도 분석 결과, 유역유출과 관련된 매개변수 중 토양습윤상태별 유출율, 침투량별 지하수유입률 및 지표수와 복류수를 분리하는 매개변수가 비교적 큰 민감도를 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10451-10456
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• 2015

Background: We defined melanoma distribution in a large series of Turkish patients and evaluated the prognostic parameters of melanomas. Materials and Methods: A total of 1574 patients' data was retrospectively collected at 18 centers in Turkey. Demographic characteristics were questioned and noted. Prognostic parametres were evaluated based on sentinel lymph node involvement. Results: Mean age was 56.7 (4-99) years. While 844 (53.6%) cases were male, 730 (46.4%) cases were female. One thousand four hundred forty-seven (92%) cases were invasive melanoma and 127 (8%) cases were in-situ melanoma. The most common histopathological form was the superficial spreading melanoma (SSM) which was found in 549 patients (37.9%). It was followed by nodular melanoma in 379 (26.2%), acral lentiginous melanoma (ALM) in 191 (13.2%) and lentigo maligna melanoma in 132 (9.1%), respectively. On univariate analysis, lymphovascular invasion (p<0.001), tumor thickness (p<0.001), histopathological subtype (p<0.001), Clark level (p=0.001), ulceration (p<0.001), ${\geq}6/mm^2$ mitosis (p=0.005), satellite formation (p=0.001) and gender (p=0.03) were found to be associated with sentinel lymph node positivity. Regression was associated with sentinel lymph node negativity (p=0.017). According to multivariate analysis, lymphovascular invasion and tumor thickness were significant independent predictive factors of SLN positivity. Patient age, tumor localization, precursor lesions, lymphocytic infiltration and neurotropism were not related with sentinel lymph node involvement. Conclusions: In this retrospective analysis, it was found that the prevalence of SSM is at a lower rate while the prevalence of ALM is at a higher rate when compared to western countries. According to Breslow index; most of the melanoma lesions' thickness were greater than 2 mm, corresponding Clark IV. Vascular invasion and tumor thickness are the most important factors for sentinel lymph node involvement.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2146-2152
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• 2015

Apios americana Medik (hereinafter Apios) has been reported to treat diseases, including cancer, hypertension, obesity, and diabetes. The therapeutic effect of Apios is likely to be associated with its anti-inflammatory activity. This study was conducted to evaluate the protective effects of Apios in animal models of acute lung injury induced by lipopolysaccharide (LPS) or pandemic H1N1 2009 influenza A virus (H1N1). Mice were exposed to LPS or H1N1 for 2-4 days to induce acute lung injury. The treatment groups were administered Apios extracts via oral injection for 8 weeks before LPS treatment or H1N1 infection. To investigate the effects of Apios, we assessed the mice for in vivo effects of Apios on immune cell infiltration and the level of pro-inflammatory cytokines in the bronchoalveolar lavage (BAL) fluid, and histopathological changes in the lung. After induction of acute lung injury, the numbers of neutrophils and total cells were lower in the Apios-treated groups than in the non-Apios-treated LPS and H1N1 groups. The Apios groups tended to have lower levels of tumor necrosis factor-a and interleukin-6 in BAL fluid. In addition, the histopathological changes in the lungs were markedly reduced in the Apios-treated groups. These data suggest that Apios treatment reduces LPS- and H1N1-induced lung inflammation. These protective effects of Apios suggest that it may have therapeutic potential in acute lung injury.

• 한국인터넷방송통신학회논문지
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• 제20권3호
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• pp.129-134
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• 2020

최근 태양전지 모듈 내부에 습기 침투 및 EVA Sheet의 배부름 현상, 프레임 Seal이 녹아내리는 현상, 설치 후 1년 지난 모듈에서 발전성능이 저하되는 현상 등이 발생하고 있다. 국내에 5~7년 이전에 설치된 태양전지 모듈에서 백화현상 및 전극 부식 현상, 절연파괴 현상 등이 나타나기 시작하여 발전성능 저하로 이어지고, 장기 신뢰성 및 장수명 기술에 대한 커다란 문제점이 대두되고 있다. 따라서 이러한 문제점들을 해결하기 위해 태양전지 모듈의 내구성 확보 및 노화 진행을 모니터링할 수 있는 기능을 포함하는 마이크로 인버터 (MiCrco Inverter Converter, 이하 MiC) 개발 및 MiC에서 모니터링 데이터를 기반으로 태양전지 모듈의 노화를 판단할 수 있는 스마트 모니터링 프로그램이 제시되고 있다. 또한, 태양전지 모듈의 모니터링 기능을 강화한 MiC와 IT 융합을 통한 체계적 운영 관리를 통한 고효율 태양광 스마트 감시 시스템이 되기 위해서는 MiC 내의 SoC (System On Chip)는 태양전지 모듈에 대한 환경정보를 복합적으로 감지하고 필요시 통신 및 제어를 수행할 수 있는 기능들이 요구되고 있다. 이러한 요구사항들을 기반으로 본 논문에서는 SoC 기반 보급형 MIC 스마트 태양광발전시스템 기술개발을 목적으로 연구하고자 한다.

• 한국세라믹학회지
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• 제45권10호
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• pp.618-624
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• 2008

Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $\beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $\beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $\beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $\beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $\beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $\beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $\beta$-TCP synthesized by fibrous monolithic process. The $\beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $\beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $\beta$-TCP bone graft substitutes.

• 대한한방소아과학회지
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• 제28권4호
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• pp.1-29
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• 2014

Objectives Dictamni Radicis Cortex extracts (DRC) has been known to suppress allergic reaction, however the cellular target of DRC and its mode of action remain unclear. The purpose of this study is to investigate the effects of Dictamni Radicis Cortex extracts on DNCB induced atopic dermatitis-like skin lesions of NC/Nga mouse. Methods This study was designed to investigate the effects of DRC extract in the DNP-IgE-induced activation of MC/9 murine mast cell lines in vitro and in the DNCB-induced activation of NC/Nga mouse in vivo. For this investigation, We examined IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ and GM-CSF mRNA expression by Real-time PCR, IL-13, MIP-$1{\alpha}$ production by ELISA analysis and manifestations of NFAT1, NFAT2, AP-1 and NF-${\kappa}B$ p65 transcription factors by western blotting in vitro. Then, we examined WBC, eosinophil and neutrophil in NC/Nga mouse, IL-5, IL-13 in serum, IFN-${\gamma}$, IL-4 in the spleenocyte culture supernatant, the absolute cell numbers of $CD4^+$, $CD8^+$, $^+Gr-1^+CD11b$, $B220^+CD23^+$ in the ALN, PBMCs and dorsal skin, IL-5, IL-13 in the dorsal skin by Real-time PCR and the distribution of mast cells by H&E and toluidine blue. Results In vitro the mRNA expression of IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$, GM-CSF and IL-13, MIP-$1{\alpha}$ production by ELISA analysis were completely abolished by DRC and the western blot analysis decreased the expression of mast cell-specific transcription factors including NFAT-1, NF-${\kappa}B$ p65. In vivo DRC oral adminstration also decreased the counts of WBC, eosinophils and inflammatory cytokines such as IL-13 and IgE in the serum. DRC oral adminstration elevated IL-4 level in the spleenocyte culture supernatant. DRC oral adminstration decreased total ALN cells, total skin cells, cell numbers of $CD4^+$, $B220^+CD23^+$ in the ALN, $^+Gr-1^+CD11b$ in the PBMCs and $CD4^+$, $CD8^+$ in the dorsal skin. The mRNA expression of IL-5, IL-13, thickness of epidermis, inflammation immune cells and mast cells were abolished by DRC in the dorsal skin. Conclusions Histological examination showed that infiltration levels of immune cells in the skin of AD-induced NC/Nga mouse were much improved by DRC oral adminstration. These results, therefore, suggest that DRC can regulate molecular mediators and immune cells that are functionally associated with atopic dermatitis induced in NC/Nga mouse, and may play an important role in recovering AD symptoms.

• Clinical and Experimental Pediatrics
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• 제54권9호
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• pp.373-379
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• 2011

Purpose: 4-1BB (CD 137) is a costimulatory molecule expressed on activated T-cells. Repression by 4-1BB is thought to attenuate Th2-mediated allergic reactions. The aim of this study was to investigate the effect of 4-1BB on allergic airway inflammation in a murine asthma model. Methods: BALB/c mice were sensitized to and challenged with ovalbumin (OVA). Hu.4-1BB-Fc was administered 1 day before the first OVA sensitization or 1 day after the second OVA sensitization. Following antigen challenge, airway responsiveness to methacholine was assessed and bronchoalveolar lavage (BAL) fluid was analyzed. Total immunoglobulin (Ig) E, OVA-specific IgE, $IgG_1$, and $IgG_{2a}$ levels in sera were measured by enzyme-linked immunosorbent assay. Lung pathology was also evaluated. Results: In mice treated with Hu.4-1BB-Fc before the first OVA sensitization, there was a marked decrease in airway hyperresponsiveness, total cell count, and eosinophil count in the BAL fluid. In addition, Hu.4-1BB-Fc treatment decreased serum OVA-specific $IgG_1$ levels and increased serum $IgG_{2a}$ level significantly compared with the corresponding levels in mice sensitized to and challenged with OVA. Hu.4-1BB-Fc-treated mice also showed suppressed peribronchial and perivascular inflammatory cell infiltration. In contrast, treatment with Hu.4-1BB-Fc 1 day after sensitization had no effect on airway hyperresponsiveness and showed less suppression of inflammation in lung tissue. Conclusion: Administration of Hu.4-1BB-Fc can attenuate airway inflammation and hyperreactivity in a mouse model of allergic airway inflammation. In addition, administration before sensitization may be more effective. These findings suggest that 4-1BB may be a useful therapeutic molecule against asthma.