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Estrogen Mediates Ischemic Damage and the Migration of Human Umbilical Cord Blood Cells

  • Kim, Jee-Yun;Yu, Seong-Jin;Kim, Do-Rim;Youm, Mi-Young;Lee, Chae-Kwan;Kang, Sung-Goo
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.71-71
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    • 2003
  • Human umbilical cord blood cells(HUCBC) are rich in mesenchymal progenitor cells, endothelial cell precursors and hematopoietic cells. HUCBC have been used as a source of transplantable stem and progenitor cells. However, little is known about survival and development of HUCBC transplantation in the CNS. Estrogen has a neuroprotective potential against oxidative stress-induced cell death so has an effect on reducing infarct size of ischemic brain. We investigated the potential use of HUCBC as donor cells and tested whether estrogen mediates intravenously infused HUCBC enter and survive in ischemic brain. PKH26 labeled mononuclear fraction of HUCBC were injected into the tail vein of ischemic OVX rat brain with or without $17\beta$-estradiol valerate(EV). Under fluorescence microscopy, labeled cells were observed in the brain section. Significantly more cells were found in the ischemic brain than in the non-ischemic brain. HUCBC transplanted into ischemic brain could migrate and survive. Some of cells have shown neuronal like cells in hippocampus, striatum and cortex tissues. These result suggest that estrogen reduces ischemic damage and increases the migration of human umbilical cord blood cells. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) though the Biohealth Products Research Center(BPRC), Inje University, Korea.

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Anaerobic Biotreatment of Animal Manure - A review of current knowledge and direction for future research -

  • Hong, Jihyung
    • Journal of Animal Environmental Science
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    • v.11 no.2
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    • pp.97-102
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    • 2005
  • Anaerobic decomposition is one of the most common processes in nature and has been extensively used in waste and wastewater treatment for several centuries. New applications and system modifications continue to be adapted making the process either more effective, less expensive, or suited to the particular waste in question and the operation to which it is to be applied. Animal manure is a highly biodegradable organic material and will naturally undergo anaerobic fermentation, resulting in release of noxious odors, such as in manure storage pits. Depending on the presence or absence of oxygen in the manure, biological treatment process may be either aerobic or anaerobic. Under anaerobic conditions, bacteria carry on fermentative metabolisms to break down the complex organic substances into simpler organic acids and then convert them to ultimately formed methane and carbon dioxide. Anaerobic biological systems for animal manure treatment include anaerobic lagoons and anaerobic digesters. Methane and carbon dioxide are the principal end products of controlled anaerobic digestion. These two gases are collectively called biogas. The biogas contains $60\~70\%$ methane and can be used directly as a fuel for heating or electrical power generation. Trace amounts of ammonia and hydrogen sulfide ($100\~300\;ppm$) are always present in the biogas stream. Anaerobic lagoons have found widespread application in the treatment of animal manure because of their low initial costs, ease of operation and convenience of loading by gravity flow from the animal buildings. The main disadvantage is the release of odors from the open surfaces of the lagoons, especially during the spring warm-up or if the lagoons are overloaded. However, if the lagoons are covered and gases are collected, the odor problems can be solved and the methane collected can be used as a fuel. Anaerobic digesters are air-tight, enclosed vessels and are used to digest manure in a well-controlled environment, thus resulting in higher digestion rates and smaller space requirements than anaerobic lagoons. Anaerobic digesters are usually heated and mixed to maximize treatment efficiency and biogas production. The objective of this work was to review a current anaerobic biological treatment of animal manure for effective new technologies in the future.

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A Study on the Comprehensive Artificial Intelligence Utilization Analysis of Online Research Platform (온라인 리서치 플랫폼의 종합적인 인공지능 활용 분석 연구)

  • Lim, Hye-Won;Nam, Won-Suk
    • Journal of the Korea Convergence Society
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    • v.11 no.10
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    • pp.187-195
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    • 2020
  • The purpose of study is to find ways to improve online research work in the near future environment. Study methods and results are as follows. First, the basis of research was secured through theoretical consideration. Second, three types were derived by collecting examples. There are reward, execution and visualization. Third, comprehensive matrix and directions were derived through case analysis. According to the study, the most frequently used phase of AI technology were analysis, visualization, execution, casting, design, and matching, The direction of use of AI can be largely divided into work efficiency, human thought assistance, and changes in research methods. But the plan phase is generally scarce. Overall, the lack of in function is provided, including AI technology. At a time when research plans are becoming more important as users and products diversify.

Design and Implementation of PLC Automatic Welding System with Power-saving (전력 절약형 PLC 자동용접 시스템 설계 및 구현)

  • Yang, Young-Joon
    • Journal of Energy Engineering
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    • v.24 no.3
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    • pp.6-12
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    • 2015
  • The welding technology has been used in almost all industries such as automotive, shipbuilding, power plants and industrial machinery. In this study, the design and implementation of PLC $CO_2$ welding automation system were investigated. For these purposes, the structure analysis for driving supporter was performed and specification of automatic voltage regulator, mutual interface of system and circuit diagram were designed in order to contrive power-saving system. As the results, the stability of design for driving supporter could be convinced by numerical analysis and PLC automatic welding system was suitable for welding automation of structural-manufacturing factory capable of producing various and small amount products. Therefore, it was confirmed that PLC $CO_2$ welding automation system could contribute to productivity, stable quality and power-saving.

Characterization of Controlled Low Strength Materials Utilizing CO2-fixation Steel Slag and Power Plant Bottom Ash (CO2고정화한 제강슬래그와 발전소 바닥재를 활용한 저강도 고유동 채움재의 특성)

  • Cho, Yong-Kwang;Kim, Chun-Sik;Nam, Seong-Young;Cho, Sung-Hyun;Lee, Hyoung-Woo;Ahn, Ji-Whan
    • Journal of Energy Engineering
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    • v.27 no.2
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    • pp.55-60
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    • 2018
  • In this study investigated the Controlled Low Strength Materials using coal ash and steel slag(KR slag) as the main material in the thermal power plant classified as waste resource. Bottom ash and KR slag are mixed at a ratio of 7: 3 to expand the use of industrial by-products through carbonate($CO_2$-fixation) reactions and inhibit the exudation of heavy metals. The results showed that the water content increased as the content of bottom ash increased. It was confirmed that as the powder content increased, the bleeding ratio decreased. Also, as the content of one kind of ordinary portland cement (OPC) decreased, activation of hydration reaction decreased and compressive strength decreased. However, when the mixed composition is appropriately adjusted, the compressive strength of 2.0 MPa required for the controlled low-strength material can be satisfied.

Quantitative Analysis of Pentachlorophenol in Paper Products (종이재 중의 Pentachlorophenol 정량 분석)

  • Hahn, Airan;Park, Jihye;Choi, Hyeongki;Hong, Sungyeap;Han, Hyechul;Jo, Byoungmuk;Oh, Jungsoo
    • Applied Chemistry for Engineering
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    • v.16 no.3
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    • pp.323-327
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    • 2005
  • Pentachlorophenol (PCP) is a representative compound of chlorophenol derivatives which are especially toxic and potentially carcinogenic compounds in the priority pollutant list of the EPA (Environmental Protection Agency, USA). The aim of this study was to investigate an analysis method of PCP in infinitesimal quantity retained in paper. PCP in paper was pretreated by solid phase extraction (SPE) in a phenyl cartridge. The extracted sample was acetylated or silylated and analyzed with GC/MS-SIM. The detection sensitivity of silylated PCP was improved significantly when compared with those of PCP itself and acetylated PCP. As a test specimen, an office paper and a wallpaper were used.

Study on the biodegradation of alternatives (four species including C8H8F9KO3S) for perfluorooctane sulfonate

  • Choi, Bong-In;Na, Suk-Hyun;Kwak, Yeong-Don;Ryu, Byung-Taek;Chung, Seon-Yong
    • Environmental Analysis Health and Toxicology
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    • v.30 no.sup
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    • pp.8.1-8.5
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    • 2015
  • Objectives The objective of this study was to evaluate the biodegradation potential of four perfluorooctane sulfonic acid (PFOS) alternatives that were developed at Changwon National University. While PFOS has been used widely in industrial and consumer products, it is known to be a persistent organic pollutant. Therefore, greener alternatives are highly desirable. Methods Biodegradation tests were run for 28 days using standard test protocols. The biochemical oxygen demand was measured daily throughout the experimental period, and the data were used to calculate the biodegradation rates. Microorganisms were isolated from the some of the tests that showed evidence of biodegradation. Results $C_8H_8F_9KO_3S$, which has the same number of carbons as the parent compound PFOS but a reduced number of fluorines, showed the highest biodegradation rate followed by $C_{10}H_8F_{13}KO_3S$. Chemical alternatives with lower number of carbons did not biodegrade readily in the experiments. Conclusions Together, these results suggest that it may be advantageous to develop PFOS alternatives with 8 carbons, the same as PFOS, but a reduced number of fluorines; as such, chemicals are more susceptible to biodegradation than the parent compound.

Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality

  • Kim, Jin-Woo;Yang, Seul-Gi;Park, Hyo-Jin;Kim, Ju Hwan;Lee, Dong-Mok;Woo, Seong-Min;Kim, Hyun-Jeong;Kim, Hyun Ah;Jeong, Jae-Hoon;Lee, Min Ji;Koo, Deog-Bon
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.2
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    • pp.207-213
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    • 2020
  • Cryopreservation is used for blastocyst preservation of most mammalian embryos and is an important technique for breeding. We aimed to compare the efficiency of the cryopreservation method using the standard Cryotop device and the ReproCarrier device, a domestic product manufactured in Korea. The efficacy of the two devices was analyzed based on the survival rate, intracellular levels of reactive oxygen species (ROS), and apoptosis of the vitrified bovine blastocysts. The survival rates of the vitrified-warmed blastocysts were similar between the ReproCarrier group (58.4 ± 17.7%) and Cryotop group (59.9 ± 14.1%). Intracellular ROS levels and apoptotic index were determined by DCFDA staining and TUNEL assay. Changes in intracellular ROS levels, number of total nuclei, and cellular apoptosis of vitrified blastocysts after cryopreservation were not significantly different between the two groups. These results indicate that the ReproCarrier device method is as effective as the standard Cryotop method for vitrification of bovine blastocysts in vitro.

Synthesis of Surface Active Properties of Destructible Surfactants with 1,3-Dioxane (1,3-디옥산을 함유한 분해성 계면활성제의 합성의 및 계면 특성)

  • Kim, Chi-Hoi;Roh, Yun-Chan;Kim, Yu-Ok;Nam, Kie-Dae
    • Journal of the Korean Applied Science and Technology
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    • v.13 no.3
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    • pp.61-71
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    • 1996
  • In acid-catalyzed acetal cyclization of long aliphatic aldehydes($R=n-C_7H_{15}$ ; $n-C_9H_{19}$ ; $n-C_{11}H_{23}$) with 1,1,1-tris(hydroxymethyl)propane, 2-alkyl-5-hydroxymethyl-5-ethyl-1,3-dioxanes were obtained. The final products, sodium 2-alkyl-5-(sulfonatedpropylethermethyl)-5-ethyl-1,3-propanesultion in the presence of sodium hydride. These compounds were a new group of destructible surfactants which were readily hydrolyzed and oxidized in natural water reservoirs. Physical properties of these new compounds involved some surface properties such as Krafft point(Kp), critical micelle concentration(cmc), surface tension of aqueous solutions near cmc(${\gamma}_{min}$), foaming power, emulsion power and hydrolysis properties were determined. The destructible surfactants containing 1,3-dioxane ring were synthesized to about $85{\pm}5.5%$ yield. The cmc values of the compounds by ring method were assumed to $0.5{\sim}5.0{\times}10^{-3}mol/L$ range and surface tensions at cmc were $29.5{\sim}33.0dyne/cm$ respectively at $25^{\circ}C$. The foaming power and foam stability were $170{\sim}230mm$ and $52{\sim}135mm$ respectively at $1{\times}10^{-2}mol/L$, foam was occurred rarely below $1{\times}10^{-3}mol/L$. The emulsion property of liquid paraffin was better than that of soybean oil. For hydrolysis property with ph and time, these compounds were decomposed within about 200minutes at $ph1{\sim}2$. Hopefully these compounds are expected to be a good O/W emulsifier that have decomposability in acid and may be used in the process which do not need foaming.

Comparison on Sanitary and Nutritional Characteristics Between Skipjack Tuna Ktsuwonus pelamis Frame and Commercial Beef Bone Extract Concentrates (어류 프레임 농축액과 시판 사골 농축액의 위생적 및 영양적 특성 비교)

  • Lim, Chi-Won;Sung, Sang Wook;Heu, Min Soo;Lee, Tae-Gee;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.467-472
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    • 2017
  • There is increasing interest in developing flavor-enriched concentrate using byproducts of skipjack tuna Ktsuwonus pelamis as a replacement for commercial beef bone extract concentrate (CBB-EC). This study was compared on sanitary and nutritional characteristics between skipjack tuna frame extract concentrate (ST-EC) and CBB-EC as a control of skipjack tuna frame extract concentrate. The moisture contents of CBB-EC and ST-EC 41.4-4.9% and 45.3%, respectively. The results of viable cell counts and E. coli assays suggested that CBB-EC and ST-EC is a safe and sanitary for use in food. There were no differences in peroxide values among CBB-ECs. The total amino acid contents in CBB-EC and ST-EC were 25.6-29.3 g/100 g and 37.9 g/100 g, respectively, and the major amino acids present glutamic acid, proline, glycine, alanine and arginine. Regardless of kinds of the mineral, mineral contents of ST-EC higher than those of CBB-EC. The major fatty acids were 16:0, 18:0 and 18:1n-9 in CBB-EC, 16:0, 18:1n-9 and 22:6n-3 in ST-EC. These results can be used as background information to develop flavor-enriched concentrates from byproducts of skipjack tuna as replacement for CBB-EC.