• Fisheries and Aquatic Sciences
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• v.26 no.3
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• Journal of Industrial Technology
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• v.20 no.A
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• pp.185-194
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• 2000

In this work we measured the total pressure of the aqueous solutions and the methanol-water solutions dissolved with inorganic salts, at $25^{\circ}C$. In organic electrolytes used in this work were $K_2SO_4$ and $(NH_4)_2SO_4$. Using the measured vapour pressures the activity coefficient of solvents and the mean ionic activity coefficient were obtained through thermodynamic relations. The activity coefficients of solvent and the mean ionic activity coefficirnt obtained in this work were fitted with Macedo's model and Acard's model. Both two models were good agreeable to the vapor pressure and the mean ionic activity coefficient for the electroyte aqueous solutions. For electrolyte /methanol/water solutions, Macedo's model had much deviation from experimental data, while Acard's model showed a good agreement with experimental data.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.302-313
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• 2016

This study conducts statistical analysis based on a survey of 300 CEOs from Korean companies in order to examine the effects of a CEO's internal?external activities (information, knowledge management, learning organization) on business innovation activity (exploitative, exploratory) and business performance. Analysis results show that learning organization activity had a significant positive (+) effect on exploitative and exploratory innovation activity. In addition, knowledge management activity lacked statistically significant effects on exploratory innovation activity. Furthermore, exploitative and exploratory innovation activity was affected by CEO's internal?external activities (information, knowledge management, learning organization) and had a significant positive (+) effect on company's business performance. but it was shown that the level of influence was different. Results of this study imply that maximizing business performance through developing innovation activity by CEO's internal?external activities (information, knowledge management, learning organization) in the company, extracting activity advantageous to company's business environment based on activity perceived in the precedent study and business strategy becomes advantageous to the attainment of business performance objectives.

• Journal of Korean Society for Atmospheric Environment
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• v.18 no.3
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• pp.205-212
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• 2002

The catalytic combustion of benzene, toluene and xylene over Pt-based catalyst was investigated in a fixed bed flow reactor system with atmospheric pressure to recycle the waste industrial catalyst for the processes of removing volatile organic compounds. According to the pretreatment condition, the properties of the waste Pt-based catalyst were characterized by XRD (X-ray diffraction) and BET (Brunauer-Emmett-Toller). In the carte of air pretreatment, 20$0^{\circ}C$ was found to be optimal, and increasing pretreatment temperature resulted in the reduction of the catalytic activity. When Pt-based catalyst pretreated at 20$0^{\circ}C$ by alto was retreated by hydrogen, the catalytic activity increased by increasing treatment temperature. In the case of HNO$_3$aqueous solution pretreatment, the catalytic activity decreased by increasing the concentration of HNO$_3$aqueous solution. The catalytic activity was seen to observe the following sequence : benzene > toluene > xylene.

• IE interfaces
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• v.16 no.2
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• pp.156-166
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• 2003

Proposed in this paper is a standard-compliant business process modeling tool which is based on the UML(Unified Modeling Language) activity diagram and produces an XPDL(XML Process Definition Language) file as an output. The XPDL is a standard process definition exchange format by WfMC (Workflow Management Coalition). To develop an UML/XPDL-based modeling tool, the mapping of modeling elements between activity diagram and XPDL format is conducted after the detailed analysis of each modeling specification. As a result of this mapping, it is revealed that modeling elements of each activity diagram and XPDL must be expanded. So new modeling elements are identified and added to each specification. Based on this mapping, the prototype system is developed, and the usefulness of the developed system is shown through the case study.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.5
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• pp.632-638
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• 2005

This paper describes a new data-handing, based on a Spatial Human Interface as human indicator, to the Spatial-Knowledge-Tags (SKT) in the spatial memory the Spatial Human Interface (SHI) is a new system that enables us to facilitate human activity in a working environment. The SHI stores human activity data as knowledge and activity history of human into the Spatial Memory in a working environment as three-dimensional space where one acts, and loads them with the Spatial-Knowledge-Tags(SKT) by supporting the enhancement of human activity. To realize this, the purpose of SHI is to construct new relationship among human and distributed networks computers and sensors that is based on intuitive and simultaneous interactions. In this paper, the specified functions of SKT and the realization method of SKT are explained. The utility of SKT is demonstrated in designing a robot motion control.

• Proceedings of the Society of Korea Industrial and System Engineering Conference
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• 2002.05a
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• pp.69-78
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• 2002

This paper examines the effects on mental fatigue of changes in the intensity of physical activity. A treadmill-equipped instrument and perception tester were used to attain several levels of physical activity In this paper, In order to determine the individual levels of physical activity of subjects, Borg-RPE scale, Heart Rate(HR) and Respiratory Quotient(RQ) were used. Also, an addition test in whole-body activity on treadmill-equipped instrument as an indicator of mental fatigue were performed. In the above experiments, the scores obtained in addition test administered before and after physical activity at each intensity level used. Restricted within the limits of this paper, the results of these tests showed that mental fatigue decreased after physical activity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.25 no.6
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• pp.42-47
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• 2002

This paper examines the effects on mental task of changes in the intensity of physical activity. A treadmill-equipped instrument and perception tester were used to attain several levels of physical activity. In this paper, in order to determine the individual levels of physical activity of subjects, Borg-RPE scale, heart rate(HR) and respiratory quotient(RQ) were used. Also, an arithmetic addition test in whole-body activity on treadmill-equipped instrument as an indicator of mental task were performed. In the above experiments, the scores obtained in arithmetic addition test administered before and after physical activity at each intensity level used. Restricted within the limits of this paper, the results of these tests showed that the performance of mental task was Increased after physical activity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.13 no.22
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• pp.99-111
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• 1990

The first national QC Circle Convention sponsored by Industry Advancement Administration in 1975 was the beginning of employees group activity from a national point view. Subsequently some events such as the first nationwide Factory Seamaul activity small Group Convention, which was supervised by the ministry of Trading and Industry and Factory Seamanulundong promotion Center, paved the way for proliferation of the group activity through all kinds of manufacturing. Although since November 1982 these two conventions has been united, many problem have been disclosed that are against the basic principle more or less. Worker's small group activity is, essentially, a trouble shooting oriented voluntary small group within their working place to improve the surroundings creatively. In practice, however, many groups are so typical and so impetuous of the material effect that it is worried to be inclined to outform rather than contents. Effective small group activities are presumed to he successful only with labor management cooperation on the basis of human-oriented management philosophy. The small group activities are also prevalent in service sector. More derivative methods have been developed and more members are willingly participating in training programs. The small group which is basically a horizontal organization unit, promotes communication within the whole organization. In consideration of the social circumstances and traditions, the flexible model of the small group activities suitable to the corporate environment, will contribute to industrial development.

• The Plant Pathology Journal
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• v.31 no.1
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• pp.61-66
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• 2015

From study of antifungal actions on the rice sheath blight by using the extract of Ginkgo biloba outer seedcoats, we found that the extracts of Ginkgo biloba outer seedcoats of all treatment concentrations had inhibited the rice sheath blight. Among them, the most effective concentration was 250 mg/l at which the growth of microbe was 26 mm and even at the packaging test, when sprayed the G. biloba outer seedcoats at the level of 250 mg/l, the damage rate of the rice sheath blight was identified as 13%. As a result investigating the antifungal activity by separating polysaccharides from G. biloba outer seedcoats, it showed that the clear zone of 14 mm or more was formed at the concentration of 250 mg/l or higher. Based on these results, we concluded that the G. biloba outer seedcoat is a natural substance with the antifungal activity on the rice sheath blight.