• 제목/요약/키워드: inductible nitric oxide (iNOS)

검색결과 1건 처리시간 0.015초

RAW264.7 세포주를 통한 복분자 30% 에탄올 추출물의 면역기능 활성증진효과 검토 (Immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) in RAW264.7 cells)

  • 조재경;최한석;김민수;김용국;김치홍;신용철;고성규
    • 대한예방한의학회지
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    • 제16권3호
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    • pp.155-165
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    • 2012
  • Objective : The object of this study is to confirm the immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) on RAW264.7 mouse macrophage cell line. Methods : RAW264.7 cells were treated with $10-500{\mu}g/mL$ RC for 24 hours. Cell viability was then measured using WST assays. Levels of intracellular NO and ROS were measured by Griess reagent and DCFH-DA staining respectively. Levels of iNOS and COX-2 mRNA was determined by RT-PCR. Secretion levels of IL-$1{\beta}$ and IL-6 cytokines were evaluated by sandwich ELISA assay. Western blot analysis was performed to measure the levels of intracellular molecules related to MAPKs pathways. Results : RC suppressed the growth of RAW264.7 cells. RC increased the production of NO and ROS. RC increased the mRNA and protein levels of COX-2 and iNOS. RC augmented the levels of secreted IL-$1{\beta}$ and IL-6 cytokines. RC increased MAPKs phosphorylation. Conclusion : In summary, our result shows that RC has inflammatory effect increasing the levels of NO, ROS and secreted cytokines and activating MAPKs. Hence, RC seems to have an immune enhancement effect.