• Molecules and Cells
• /
• 제39권12호
• /
• pp.877-887
• /
• 2016

Tazarotene-induced gene 1 (TIG1) is a retinoic acid-inducible protein that is considered a putative tumor suppressor. The expression of TIG1 is decreased in malignant prostate carcinoma or poorly differentiated colorectal adenocarcinoma, but TIG1 is present in benign or well-differentiated tumors. Ectopic TIG1 expression led to suppression of growth in cancer cells. However, the function of TIG1 in cell differentiation is still unknown. Using a yeast two-hybrid system, we found that transmembrane protein 192 (TMEM192) interacted with TIG1. We also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins, including Beclin-1 and LC-3B. The silencing of TMEM192 reduced the TIG1-mediated upregulation of autophagic activity. Furthermore, silencing of either TIG1 or TMEM192 led to alleviation of the upregulation of autophagy induced by all-trans retinoic acid. Our results demonstrate that the expression of TIG1 leads to cell autophagy through TMEM192. Our study also suggests that TIG1 and TMEM192 play an important role in the all-trans retinoic acid-mediated upregulation of autophagic activity.

• 농업과학연구
• /
• 제38권1호
• /
• pp.7-10
• /
• 2011

In order to develop the new Miscanthus varieties for biomass, characteristics of collected materials were surveyed. Also, they were treated with S-azide(Sodium-azide) and DES(dethyl sulfate) for promising lines and 0.2% Colchicine for polyploidy, respectively. CNU-J1 line induced with chemical, DES and S-azide treatment showed shorter than others in stem height and stem diameter. CNU-N1 line treated with 10 mM DES showed earlier than non-treated lines in flowering as 7days. Among selected lines, the CNU-J1 line was crossed with DB-1 selected as a superior line and their hybrid was expected to be high in biomass than others. Especially, CNU-W1 line treated with 2.5~5.0 mM DES showed heading in M1 generation.

• Bulletin of the Korean Chemical Society
• /
• 제23권1호
• /
• pp.35-40
• /
• 2002

Photoreaction of guest organic anions in layered organic-inorganic hybrid materials was investigated. The layered hybrids were synthesized by an anion-exchange reaction of $[LiAl_2(OH)_6]Cl{\cdot}yH_2O$ layered double hydroxide with aqueous (Z,Z)- and (E,E)-muconates under inert atmospheric condition, to give new organicinorganic hybrids of $[LiAl_2(OH)_6]_2[(Z,Z)-C_6H_4O_4]{\cdot}zH_2O$ and $[LiAl_2(OH)_6]_2[(E,E)-C_6H_4O_4]{\cdot}H_2O$, respectively. The basal spacings calculated by XRPD of intercalates indicate that muconate anions have almost vertical arrangements against the host $[LiAl_2(OH)_6]^+$ lattices in the interlayer of organic-inorganic hybrid materials. When UV light was irradiated on the suspension of $[LiAl_2(OH)_6]_2[(Z,Z)-C_6H_4O_4]{\cdot}zH_2O$, the (Z,Z)-muconate anions of the gallery space of hybrids were polymerized in the aqueous media while it was isomerized into more stable (E,E)-muconate in the methanollic suspension in the presence of catalytic amount of molecular iodine. All the products were characterized using elemental analysis, TGA, XRPD, FT-IR, $^1H$ NMR and $^{13}C$ CP-MAS NMR.

• Earthquakes and Structures
• /
• 제12권3호
• /
• pp.285-296
• /
• 2017

Base isolation is a quite practical control strategy for enhancing the response of structural systems induced by strong ground motions. Due to the dynamic effects of base isolation systems, reduction in the interstory drifts of the superstructure is often achieved at the expense of high base displacement level, which may lead to instability of the structure or non-practical designs for the base isolators. To reduce the base displacement, several hybrid structural control strategies have been studied over the past decades. This study investigates a particular strategy that employs Tuned Mass Dampers (TMDs) for improving the performance of base-isolated structures and unlike previous studies, specifically focuses on the effectiveness of this hybrid control strategy in structures that are equipped with nonlinear base isolation systems. To consider the nonlinearities of base isolation systems, a Bouc-Wen model is selected and nonlinear dynamic OpenSees models are used to perform several time-history simulations in time and frequency domains. Through these numerical simulations, the effects of several parameters such as the fundamental period of the structure, dynamic properties of the TMD and isolation systems and properties of the input ground motion on the behaviour of TMD-structure-base isolation systems are examined. The results of this study provide a better insight into the performance of linear shear-story structures with nonlinear base isolators and show that there are many scenarios in which TMDs can still improve the performance of these systems.

• 제어로봇시스템학회논문지
• /
• 제7권11호
• /
• pp.912-917
• /
• 2001

In this study, we define the measuring method of crank angle precisely using an event and perform a study on the hardware structure and software algorithms which is applicable for the commercial engine. Also we developed a Computer-ECU(Personal computer based electronic control unit) using a computer and a microprocessor, for performing the ignition at a desire position(angle) and for controlling a duty ratio a pulse for ISC(Idle speed control). We applied these algorithms to the modeling which is induced a concept of event and got a better result than a conventional ECU in the state of transient as a result of performing air fuel ratio control in a commercial engine. This technique can be used for the back to improve ECU performance. It the present type of Hybrid I. C voltage regulator is altered to the new type of regulator, we will be surely able to reduce the production cost as well as simplify the design of alternator\`s rear bracket and rectifier part because of the removal of trio diode. Experiment is taken by MS-R004.

• Journal of Microbiology and Biotechnology
• /
• 제15권5호
• /
• pp.1039-1046
• /
• 2005

The salt resistance of antibacterial activity and synergistic effect with clinically used antibiotic agents are critical factors in developing effective peptide antibiotic drugs. For this reason, we investigated the resistance of antibacterial activity to antagonism induced by NaCl and $MgCl_2$ and the synergistic effect of P20 with cefotaxime. P20 is a 20-residue synthetic peptide derived from a cecropin A (CA)-melittin(ME) hybrid peptide. In this study, P20 was found to have potent antibacterial activity against clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) strains without hemolytic activity against human erythrocytes. The combination study revealed that P20 in combination with cefotaxime showed synergistic antibacterial activity in an energy-dependent manner. We also confirmed the synergism between P20 and cefotaxime by fluorescence-activated flow cytometric analysis by staining bacterial cells with propidium iodide (PI) and bis-(1,3-dibutylbarbituric acid) trimethine oxonol (BOX). This study suggests that P20 may be useful as a therapeutic antibiotic peptide with synergistic effect in combination with conventional antibiotic agents.

• Earthquakes and Structures
• /
• 제11권4호
• /
• pp.649-664
• /
• 2016

A seismic damaged bridge may be hit again by a strong aftershock or another earthquake in a short interval before the repair work has been done. However, discussions about the impact of the unrepaired damages on the residual earthquake resistance of a steel bridge are very scarce at present. In this paper, nonlinear time-history analysis of a steel arch bridge was performed using multi-scale hybrid model. Two strong historical records of main shock-aftershock sequences were taken as the input ground motions during the dynamic analysis. The strain response, local deformation and the accumulation of plasticity of the bridge with and without unrepaired seismic damage were compared. Moreover, the effect of earthquake sequence on crack initiation caused by low-cycle fatigue of the steel bridge was investigated. The results show that seismic damage has little impact on the overall structural displacement response during the aftershock. The residual local deformation, strain response and the cumulative equivalent plastic strain are affected to some extent by the unrepaired damage. Low-cycle fatigue of the steel arch bridge is not induced by the earthquake sequences. Damage indexes of low-cycle fatigue predicted based on different theories are not exactly the same.

• 한국전자파학회논문지
• /
• 제16권2호
• /
• pp.174-181
• /
• 2005

이 논문에서는 숲 속에 숨어있는 타겟의 산란 특성을 계산할 수 있는 효율적인 모델을 계발한다. 이 산란모델은 숲의 산란 특성을 계산하기 위해 프랙탈 기하학과 single scattering 이론을 사용하는 channel model과 타겟 자체의 산란 특성을 계산하기 위해 physical optic(PO) 근사법을 사용하는 hybrid 모델이다. 그리고 전체 모델의 계산량을 줄이기 위해 가역정리를 사용하여 간단하게 타겟과 숲 사이의 상호 작용을 계산한다.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2000년도 춘계학술대회논문집
• /
• pp.280-285
• /
• 2000

With recent development of technology of high stiffness material and the structural design, the construction of high rise structures such as tall building, tower has increased. The more flexible and slender structure is vulnerable to the internal and external dynamic loads induced by earthquake, wind and traffic load. There have been great effort and many researches to minimize the influence of dynamic loads on the structure. The traditional and stable method, the application of the passive damper, is not able to comply with various dynamic loads, while the mass damper which active control technology is integrated can effectively comply with load types. Therefore, the application of active control of huge structures with AMD(Active mass damper) or HMD(Hybrid Mass damper) is increasing. Up to now, most of actuators are servomotor and hydraulic actuator. But it is known that the electromagnetic actuator applies non contacting control force, which makes the control system easier with no characteristic change depending on time. In this paper, Hybrid mass damper with electromagnetic actuator was designed and applied to building scaled structure. The performance of designed HMD tested by shake table test is included.

• 한국환경과학회지
• /
• 제7권5호
• /
• pp.599-605
• /
• 1998

The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.