• Proceedings of the KIEE Conference
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• 2002.11a
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• pp.167-169
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• 2002

Selective, highly stable determination of serotonin was achieved in cyclic voltammetric measurement carried out at electrochemically treated conductive boron-doped diamond electrode. Boron-doped diamond electrodes were prepared on single crystal Si wafers by microwave plasma chemical vapor deposition and $B_2O_3$ was dissolved in acetone/methanol(9:1) mixture solution so that the B/C weight ratio ca. $10^4ppm$. Serotonin is a kind of indoleamines, which secreted from adrenal marrow cells. The serious problem to detection of serotonin is the interference phenomena of electroactive constituent, including AA. In this study, electrochemical treatment of HDD was carried out to discriminate between serotonin and AA responses. Experimental results showed that the peak potential of AA oxidation shift to the positive direction and the oxidation peak of serotonin was unchanged.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.85.2-85.2
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• 2003

In vivo aromatic ${\beta}$-carbolines, such as harman and norharman, may easily be formed by cyclization of indoleamines with e.g. aldehydes. Because of the structural similarity to MPTP, ${\beta}$-carbolines have been proposed as endogenous toxins. In this study, we have investigated the effects of harman and norharman on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells. Treatment of PC12 cells with harman and norharman showed 48.8% and 49.5% inhibition of dopamine content at a concentration of 20 ${\mu}$M and 100 ${\mu}$M for 48h. (omitted)

• Applied Biological Chemistry
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• v.19 no.2
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• pp.65-69
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• 1976

Methanolic extract of the common reed sprouts (Phragmites communis Trin.), unincubated or incubated at $27^{\circ}C$ in the dark, was subjected to thin layer chromatography for the indole compounds. Serotonin, tryptophan and tryptamine were the major indoles in the unincubated sprouts. At early stage of incubation of sprouts, however, serotonin and tryptamine decreased while other indoleamines increased. Tryptophan remained constant throughout the incubation periods. From the observation in the present study, it was speculated that serotonin be synthesized from tryptophan via tryptamine and in turn metabolized to bufotenine.

• Analytical Science and Technology
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• v.8 no.2
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• pp.215-220
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• 1995

A simple, efficient and sensitive method was described for the simultaneous determination of catecholamine, indoleamine and related metabolites from the homogenates of the rat brain striatum by HPLC-ECD. The optimum mobile phase on a reverse phase $C_{18}$ column was 35mM sodium acetate buffer(included 10mM citric acid, 0.13mM $Na_4EDTA$, 0.58mM SOS, pH3-4):MeOH=85:15. The column temperature was $30^{\circ}C$. Dopamine(DA), 3, 4-dihydroxyphenyl acetic acid(DOPAC), homovanilic acid(HVA), 5-hydroxyindole acetic acid(5-HIAA), serotonin(5-HT) and noradrenaline(NA) could be separated and analysed to very small amount. The detection limits of this method were 2~10pg per injection for all components. The effects of age and sex of rat on the contents of the catecholamines and their metabolites in rat brain striatum were studied. The levels of DA and 5-HT contents of the 7 week old female rats were higher than those of the 7 week old male rats. As the age of rat increases, the contents of DOPAC increased significantly.