• 제목/요약/키워드: indole 3-acetic acid

검색결과 231건 처리시간 0.023초

Production of Auxins and Auxin-like Compounds by Ginseng Growth-promoting Bacterium Pseudomonas fluorescens KGPP 207

  • Ten, Leonid N.;Lee, Mi Ja;Lee, Mee-Kyoung;Park, Hoon;Yoon, Jong Hyuk
    • Journal of Applied Biological Chemistry
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    • 제43권4호
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    • pp.264-268
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    • 2000
  • High activity of acidic ethylacetate extract from the culture supernatant of ginseng growth-promoting bacterium Pseudomonas fluorescens KGPP 207 and its fractions were demonstrated through wheat coleoptile bioassay. The following auxins and auxin-like compounds were identified in these fractions by combined gas chromatography-mass spectrometry: indole-3-acetic acid, indole-3-acetic acid methyl and ethyl ester, indole-3-butyric acid, indole-3-lactic acid and its methyl ester, indole-3-propionic acid, indole-3-pyruvic acid, p-hydroxyphenyl acetic acid, p-hydroxyphenyl acetic acid methyl and ethyl ester, phenyl acetic acid and its methyl ester. The bacterium KGPP 207 belongs to the strain of P. fluorescens which produces plant growth regulators and its beneficial effect on the ginseng growth may be due to the formation of the identified compounds.

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Biosynthesis of Indole-3-acetic Acid in Ginseng Growth-promoting Pseudomonas fluorescens KGPP 207

  • Leonid, N.Ten;Lee, Mee-Kyoung;Lee, Mi Ja;Park, Hoon
    • Journal of Applied Biological Chemistry
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    • 제43권4호
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    • pp.269-272
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    • 2000
  • The ginseng growth-promoting bacterium Pseudomonas fluorescens KGPP 207 synthesized indole-3- acetic acid (IAA) from L-tryptophan, indole-3-pyruvic acid (IPyA), and indole-3-acetaldehyde (IAAld), but not from indole-3-acetamide (lAM) and other intermediates of various IAA biosynthetic pathways in the experiment with indole compound supplemented cell suspensions. TLC, HPLC, and GC-MS analyses revealed the presence of IPyA, indole-3-ethanol, indole-3-lactic acid and its methyl ester, IAA and its methyl, and ethyl esters in the culture supernatant of the bacterium. IAAld was detected in the supernatant using sodium bisulfite and TLC. The results indicate that unlike gall-forming bacteria which can synthesize IAA by lAM, the indole-3-pyruvic acid pathway is the route for IAA biosynthesis in this beneficial strain of P. fluorescens.

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Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1

  • Romasi, Elisa Friska;Lee, Jinho
    • Journal of Microbiology and Biotechnology
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    • 제23권12호
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    • pp.1726-1736
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    • 2013
  • Biosynthesis of indole-3-acetic acid (IAA) via the indole-3-pyruvic acid pathway involves three kinds of enzymes; aminotransferase encoded by aspC, indole-3-pyruvic acid decarboxylase encoded by ipdC, and indole-3-acetic acid dehydrogenase encoded by iad1. The ipdC from Enterobacter cloacae ATCC 13047, aspC from Escherichia coli, and iad1 from Ustilago maydis were cloned and expressed under the control of the tac and sod promoters in E. coli. According to SDS-PAGE and enzyme activity, IpdC and Iad1 showed good expression under the control of $P_{tac}$, whereas AspC was efficiently expressed by $P_{sod}$ originating from Corynebacterium glutamicum. The activities of IpdC, AspC, and Iad1 from the crude extracts of recombinant E. coli Top 10 were 215.6, 5.7, and 272.1 nmol/min/mg-protein, respectively. The recombinant E. coli $DH5{\alpha}$ expressing IpdC, AspC, and Iad1 produced about 1.1 g/l of IAA and 0.13 g/l of tryptophol (TOL) after 48 h of cultivation in LB medium with 2 g/l tryptophan. To improve IAA production, a tnaA gene mediating indole formation from tryptophan was deleted. As a result, E. coli IAA68 with expression of the three genes produced 1.8 g/l of IAA, which is a 1.6-fold increase compared with wild-type $DH5{\alpha}$ harboring the same plasmids. Moreover, the complete conversion of tryptophan to IAA was achieved by E. coli IAA68. Finally, E. coli IAA68 produced 3.0 g/l of IAA after 24 h cultivation in LB medium supplemented with 4 g/l of tryptophan.

CLINICAL EFFICACY EVALUATION OF O/W EMULSION CONTAINING INDOLE-3-ACETIC ACID WITH THE FUNCTION OF ANTI-WRINKLE

  • Yang, Jae-Hun;Lee, Sun-Young;Han, Yang-Su;Jung, Hye-Sun;Kim, Ju-Ho;Park, Kwon-Ho;Choy, Jin-Ho
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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    • pp.219-238
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    • 2003
  • Indole-3-acetic acid (IAA) molecule has been successfully encapsulated in biocompatible inorganic matrix in order for the cosmetic application such as superfacial fine line reduction. The encapsulation was realized through chemical reaction involving simultaneous formation of inorganic lattice and 1M giving rise to an 1M-inorganic nanohybrid (IAA-brid) which shows excellent storage stability and sustained releasing property of indole-3-acetic acid. The clinical efficacy of essence cream containing IAA-brid as anti-wrinkle formulation was also carefully evaluated by measuring the roughness of the skin replica before and after treatment. Upon administration of the cream on the eye-area, the fine-line is drastically reduced.

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Liquid Chromatography-Mass/Mass Spectrometry (MS)와 Gas Chromatography-MS를 이용한 농축 액상 비료제품 중 Indole-3-acetic acid 및 Indole-3-butyric acid 정량분석능 비교 (Comparison of Liquid Chromatography-Mass/Mass Spectrometry (MS) and Gas Chromatography-MS for Quantitative Analysis of Indole-3-acetic acid and Indole-3-butyric acid from the Concentrated Liquid Fertilizer)

  • 김진효;박종민;최근형;박연기;임건재;김두호;권오경
    • Journal of Applied Biological Chemistry
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    • 제56권1호
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    • pp.53-57
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    • 2013
  • 비료제품에는 의도적 혹은 비의도적으로 농약으로 등록된 indole-3-acetic acid (IAA) 및 indole-3-butyric acid (IBA)성분에 대한 잔류 가능성이 꾸준히 제기되고 있으나, 생장조정제가 아닌 비료제품에서 mg/L 수준의 옥신류 잔류분석법이 마련되어 있지 않았다. 따라서, 본 연구에서는 생체시료에 미량 잔류하는 IAA 및 IBA 분석에 사용되어 온 liquid chromatography-mass/mass spectrometry (LC-MS/MS)와 gas chromatography-MS 기기분석법을 활용하여 농축 액상비료제품에 적용할 수 있는지 조사하였으며, 액상 비료제품에 적용 가능한 정밀기기 분석법을 개발하고자 하였다. 수용액 상태의 시료에서 식물 생장호르몬인 IAA와 IBA를 가장 손쉽게 정제할 수 있는 방법으로 hydrophile-lipophile balance (HLB) solid phase extraction를 활용하였으나, 제한된 조건에서 LC-MS/MS를 통한 정량분석은 적합한 회수율을 확보하지 못하였고, 정성분석만 가능함을 확인하였다. 반면, 비료제품 250배 희석액을 사용하여 HLB 정제, trimethylsilyl chloride을 이용한 methylation을 통한 GC-MS 분석에서는 검출한계 1.4 mg/L과 93-107%의 회수율로 액상 비료제품에서 IAA와 IBA 정량분석법을 확립할 수 있었다.

항염증제인 Etodolac 유도체의 합성에 관한 연구 (Studies on the Synthesis of Etodolac Derivatives as Potential Anti-inflammatory Agents)

  • 조훈;정용석;장향동;류성렬
    • 공업화학
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    • 제10권1호
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    • pp.135-137
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    • 1999
  • 새로운 인돌 유도체인 항염증제를 합성하기 위하여 ${\alpha}$-benzoyl-1-ethyl-1,3,4,9-tetrahydro-8-ethyl-9-(N-benzoyl)pyrano[3,4-b]indole-1-acetic acid methyl ester(10)을 합성하였다. 출발물질은 7-ethylindole과 oxalyl chloride로 하여 4단계로 이루어졌다. 셋째 단계는 borontrifluoride diethyl etherate을 사용하여 66%로 고리화반응을 하였으며, 환원반응과 고리화반응도 효율적으로 단순화하였다. 최종생성물인 ${\alpha}$-benzoyl-1-ethyl-1,3,4,9-tetrahydro-8-ethyl-9-(N-benzoyl)pyrano[3,4-b]indole-1-acetic acid methyl ester는 4단계에서 얻은 methyl 1,8-dimethyl-1,3,4,9-tetrahydropyrano[3,4-b]indole-1-acetate(etodollic acid methyl ester)를 benzoyl chloride와 반응하여 66%의 수율로 얻었다.

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GC/MSD를 이용한 콩나물에서의 Indole-3-acetic acid 분석 (Analysis of Indole-3-acetic acid from bean sprouts by GC/MSD)

  • 이자영;한일근;이상윤;허우덕;여익현
    • 분석과학
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    • 제8권3호
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    • pp.375-381
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    • 1995
  • 간단한 용매추출 조작에 의한 콩나물에서의 Indole-3-acetic acid(IAA)분석에 관하여 연구하였다. 콩나물 재배시 성장촉진제로 사용되는 인돌비액제의 주성분인 IAA의 추출은 80% Acetone 100ml와 Methanol 50ml 혼합 용액으로 하였으며, 추출 농축된 시료를 14% $BF_3$/methanol로 methyl ester화하여 GC/MSD로 분석하였다. 본 분석방법의 회수율은 약 80%로서 우수하였으며, 이 분석방법을 이용하여 시중에 유통중인 포장 콩나물에서의 IAA분석을 시도하였다.

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녹두발아 중에 생성되는 Indole화합물에 대하여 (Some Observations on the Indole Compounds in Mung Bean Sprauts)

  • 이춘영;조인호;김인수
    • Applied Biological Chemistry
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    • 제15권1호
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    • pp.1-6
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    • 1972
  • The naturally occurring indole derivatives in mung bean (Phaseolus vidissinus) sprouts were investigated by means of paper and thin layer chromatographic techniques. The results can be summerized as follows: 1. The mung bean sprouts are richer in free tryptophan than other plant species. 2. Indole ethanol and indole lactic acid were identified. 3. The content of indole ethanol was more than that of indole acetic acid. This result appeared to support the idea that indole ethanol is the storage product of indole acetic acid.

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저장 온도와 시간이 신선한 돈육 삼겹 부위로부터 추출한 휘발성 화합물의 조성에 미치는 영향 (Evaluation of Various Storage Temperatures and Times on the Composition of Volatile Compounds Extracted from Fresh Pork Belly)

  • 박성용;진구복;유승석
    • 한국축산식품학회지
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    • 제26권4호
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    • pp.441-446
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    • 2006
  • 신선한 돈육 삼겹살로부터 방향족(6), 알데하이드(6), 카르복시산(5), 알코올(4), 케톤(4), 알칸(4), 알켄(1)및 아민류(1)가 동정되었다. 이중 11개 화합물(1-butanol, propane, 2-butanol, 3-hydroxy-2-butanonl, acetic acid, 3-methyl-1-butanol, 1-pentanol, phenol, 2-pentyl-furan, indole, 2-dodecanone)만이 저장기간과 저장 온도사이에 상호작용이 존재하였다. (p<0.05). 삼겹살에 있어서 $4^{\circ}C$ 저장시 hexanal과 hexadecanal과 같은 알데하이드 계통의 화합물이 주로 발생되었고 반면에 $20^{\circ}C$ 저장시에는 phenol과 indole과 같은 방향족 화합물과 1-butanol과 2-butanol과 같은 알콜류 화합물이 주로 생성되었다. 동정된 휘발성 화합물중 1-butanol, 2-butanol, 3-hydroxy-2-butanone, acetic acid, phenol, indole은 저장기간에 따른 차이를 보인 화합물들이며, 1-butanol, 2-butanol, 3-hydroxy-2-butanone, acetic acid, indole 그리고 2-dodecanone과 같은 휘발성 화합물들은 모두 $20^{\circ}C$에서 저장된 삼겹살에서만 검출되었다. 이러한 결과는 돈육의 삼겹 부위가 $20^{\circ}C$의 저장에서는 방향족 화합물이, $4^{\circ}C$의 저장에서는 알데하이드류가 주로 생성된 것처럼 저장 온도에 따라 생성되는 휘발성 화합물이 차이가 있고 저장기간이 경과함에 따라 달라짐을 시사한다.

Indole-3-acetic acid (IAA) 생성 Arthrobacter sp.의 분리 및 식물 생육촉진 효과 (Isolation of Indole-3-acetic acid (IAA) producing Arthrobacter sp. and plant growth promotion effect)

  • 김다솜;신호용;한송이
    • 한국응용과학기술학회지
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    • 제39권6호
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    • pp.831-838
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    • 2022
  • 농업 재배지 토양으로부터 auxin 생성세균 KSD16, KSD33 그리고 KSD36를 분리하였다. 분리균주 KSD16, KSD33 그리고 KSD36 는 16S rRNA 유전자 계통분석을 통해 Arthrobacter 속으로 분류되었다. 이들 균주는 R2A배지에 0.1% L-tryptophan를 첨가한 배지에서 28℃, 48 시간 배양한 결과, auxin의 일종인 indole-3-acetic acid (IAA)를 204.4 mg L-1 생성하는 것으로 확인되었다. IAA 생성세균의 녹두종자 발아에 미치는 영향을 확인한 결과, Arthrobacter 속 균주 KSD16, KSD33 그리고 KSD36 는 대조군에 비해 뿌리길이와 발근수가 증가하였다. Arthrobacter 속 균주의 녹두 성장촉진 효과를 확인한 결과, 녹두의 발아율이 대조군보다 73.4 % 증가하는 특징을 나타내었다.