• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.563-565
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• 1992

The objective of this study is to compare the qualitative and quantitative properties of paneer prepared from buffalo and cow milk. Paneer from buffalo milk had higher total solids as compared to cow. Moreover significant differences were recorded in fat and protein contents among tested samples of milk, cheese and whey respectively. The results of the organoleptic evaluations showed that paneer made from cow milk was liked more as compared to buffalo.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.2
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• pp.142-148
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• 2020

The research work was undertaken to determine an effective fertilization medium, sperm separation method and sperm capacitating agent for optimum in vitro fertilization (IVF) rates of indigenous zebu cow oocytes. In experiment 1, tissue culture medium (TCM 199), Tyrode's albumin lactate pyruvate (TALP) and Brackett and Oliphant (BO) medium were used as basic medium for IVF of oocytes of indigenous zebu cows. In experiment 2, three sperm separation methods namely centrifugation, swim up and percoll gradient methods were used for separation of motile and viable spermatozoa for IVF. In experiment 3, for capacitation of spermatozoa, IVF medium supplemented with the heparin, mixture of penicillamine, hypotaurine and epinephrine (PHE) or the combination of heparin with PHE were used for fertilization. In vitro culture (IVC) of presumptive zygotes was done in modified synthetic oviduct fluid (mSOF) medium using standard procedure 24 h after sperm-oocytes co-culture. The cleavage rate was determined to evaluate the efficacy of fertilization medium, sperm separation method and sperm capacitating agent 24 h after IVC. The cleavage rate was higher in oocytes fertilized in TALP (63.3%) than in TCM 199 (47.5%) (p < 0.05). The cleavage rate was higher in oocytes fertilized by spermatozoa separated by percoll gradient method (62.3%) than by centrifugation (51.6%) (p < 0.05). The cleavage rate of oocytes was higher when insemination was done with spermatozoa capacitated in TALP supplemented with heparin and PHE (61.3%) compared to control (40.9%) (p < 0.05). In conclusions, TALP based medium and percoll gradient sperm separation followed by capacitation with combination of heparin and PHE are suitable for IVF of indigenous zebu cow oocytes in Bangladesh.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.79-86
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• 2022

Reproductive disorders in cows cause economic loss in livestock farms. This study was carried out to investigate the incidence of endometriosis in the uterine of Korean indigenous cow (Hanwoo). In the present study, the uterine of 25 cows was provided by the slaughterhouse. As a result on a visual examination of the uterus, 18 out of 25 were visually normal, and 7 uteruses (28%) appeared rather pale and showed purulent or mucosal symptoms in Uterine horn. However, the results of hematological analysis showed that both RBC and WBC were normal and showed no signs of systemic inflammation, indicating 7 cows showed asymptomatic endometriosis. The inflammatory uterus (28%) showed a wide range of pathological conditions that elicit an inflammatory response, such as serous exudate and bleeding. Histological and microscopic analysis in the inflammatory group demonstrated that there was swelling of the uterine glands, and neutrophil, basophil, and lymphocyte appeared in the uterine gland. Moreover, plasma cells and hemosiderin-laden macrophages were increased in the endometrial stroma, which lead to inhibit pregnancy by suppression of the synthesis of pregnancy hormones, and the appearance of hemosiderin-laden macrophages is an indicator of intracellular bleeding. In summary, hematologically, it is a normal diagnosis in Korean indigenous cows, however, when the uterus was extracted and investigated microscopically, the asymptomatic endometriosis were evident. In order to achieve the goal of healthy cow management and breeding within 2 weeks after birth, cows' uterus should be washed, disinfected, and through thorough the hygiene management, it aims to prevent asymptomatic endometriosis to produce healthy offspring and reduce the breeding interval.

• Journal of Embryo Transfer
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• v.28 no.4
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• pp.319-324
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• 2013

This study was conducted among 120 different breed cows at selected areas of Sirajgonj district from March to July 2010 to compare the reproductive performance of crossbred and Desi cows at farmer's level. The results showed that the average daily milk yield of Desi, Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi cows was $2.3{\pm}0.2$, $4.9{\pm}0.9$, $6.0{\pm}1.0$ and $5.7{\pm}0.9$ liters, respectively. The milk yield of crossbred cows ($5.5{\pm}0.6$ liters/day) was significantly (p<0.01) higher than Desi cows ($2.3{\pm}0.2$ liters/day). The average age at puberty of Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi was significantly (p<0.01) lower than that of Desi breed. The crossbred cows had significantly (p<0.01) lower pubertal age ($20.4{\pm}1.2$) than Desi ($25.9{\pm}1.1$). The age at first calving in Desi cows was significantly (p<0.01) higher ($37.6{\pm}1.1$ months) than crossbred cows ($31.2{\pm}1.3$ months). The average gestation length of Desi, Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi was $289.9{\pm}1.4$, $285.0{\pm}0.0$, $285.0{\pm}4.2$ and $282.1{\pm}2.4$ days, respectively. It is suggested that the overall reproductive performance of Friesian ${\times}$ Desi, Jersey ${\times}$ Desi and Shahiwal ${\times}$ Desi cows were better than that of Desi cow.

• Journal of Embryo Transfer
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• v.30 no.4
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• pp.327-333
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• 2015

The objectives of the present study were to select an effective basic medium including its hormone and protein supplementation for IVM of oocytes of indigenous zebu cows. The ovaries of cows were collected from slaughter house and the follicular fluid was aspirated from 2 to 8 mm diameter follicles. The COCs with more than 3 cumulus cell layers and homogenous cytoplasm were selected for maturation. The oocytes were matured in media for 24 hrs at $39^{\circ}C$ with 5% $CO_2$ in humidified air. The maturation of oocytes was evaluated by examining the presence of first polar body under microscope. An efficient basic medium was determined after culturing COCs in either TCM 199 or SOF medium in Experiment 1. An efficient hormone supplementation was determined after culturing COCs in either FSH or gonadotrophin supplemented TCM 199 in Experiment 2. An efficient protein supplementation was determined after culturing COCs in either FBS or Oestrous cow serum (OCS) supplemented TCM 199 in Experiment 3. The oocyte recovery rate per ovary was 3.35. The overall rate of IVM was 74.6%. The maturation rate was $75.5{\pm}3.9$ and $62.2{\pm}20.2%$ in TCM and SOF medium, respectively (P>0.05). The maturation rate of oocytes was significantly higher ($76.6{\pm}13.2%$) in FSH supplemented medium than gonadotrphin supplemented counterpart ($69.7{\pm}10.8%$) (P<0.05). The maturation rates of oocytes were $81.7{\pm}12.9$ and $85.7{\pm}12.7%$ in medium supplemented with FBS and OCS, respectively (P>0.05). In conclusions, both TCM 199 and SOF supplemented with either FBS or OCS, and FSH may be used as medium for IVM of indigenous zebu oocytes in Bangladesh.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1039-1046
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• 2004

A model Indigenous Thai beef cattle breeding structure consisting of nucleus, multiplier and commercial units was used to evaluate the effect of changes in heritabilities of and genetic correlations between adaptation traits on genetic gain and profitability. A breeding objective that incorporated adaptation was considered. Two scenarios for improving both the production and the adaptation of animals where also compared in terms of their genetic and economic efficiency. A base scenario was modelled where selection is for production traits and adaptation is assumed to be under the forces of natural selection. The second scenario (+Adaptation) included all the information available for base scenario with the addition of indirect measures of adaptation. These measures included tick count (TICK), faecal egg count (FEC) and rectal temperature (RECT). Therefore, the main difference between these scenarios was seen in the records available for use as selection criteria and hence the level of investments. Additional genetic gain and profitability was generated through incorporating indirect measures of adaptation as criteria measured in the breeding program. Unsurprisingly, the results were sensitive to the changes in heritabilities and genetic correlations between adaptation traits. However, there were more changes in the genetic gain and profitability of the breeding program when the genetic correlations of adaptation and its indirect measures were varied than when the correlations between these measures were. The changes in the magnitudes of the genetic gain and profit per cow stresses the importance of using reliable estimates of these traits in any breeding program.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.461-464
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• 1996

The data on milk yield of 1837 healthy dairy cows of different genetic groups raised in Central Cattle Breeding and Dairy farms, Savar, Dhaka for the period from 1978 to 1992 were analyzed from first to 6th lactation. The mean (SE) milk yields of 100 days, 305 days, total lactation and total life time ($928{\pm}48.6kg$, $2,188{\pm}201.81kg$, $2,661{\pm}385.46kg$ and $11,134{\pm}2,916kg$) respectively, were in pure bred Friesian found to be highest among all genetic groups. The corresponding means in indigenous cows, $334{\pm}12.92kg$, $946{\pm}64.44kg$, $635{\pm}16.31kg$ and $3,934{\pm}402.21kg$ were the lowest. Among the $F_1$, $F_2$ and $F_3$ cross-breds, the $F_1$ half bred Friesian showed the highest performance in milk yield. The half bred Friesian in successive generations yielded low milk. Similar trend was obtained incase of cross-bred with other breeds such as Jersey, Sindhi and Sahiwal. From the over all results, it can be concluded that the performance in respect to milk yield of pure bred Friesian and their $F_1$ half bred with indigenous and Sahiwal were superior over the $F_2$ or $F_3$ cross-breds between Fresian and any other breed irrespective of proportion of blood. The performance in respect to production of indigenous local cow was found to be almost similar with that of Sahiwal breed.

• Journal of Embryo Transfer
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• v.17 no.2
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• pp.123-128
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• 2002

The present study aimed at determining the effective dose of Folltropin, a follicle timulating hormone (FSH), on superovulation in indigenous cows of Bangladesh. Fifteen regularly cycling 5～7 years old dry cows, weighing 200～250 kg with 2.5～3.0 body condition scores (BCS) were divided into three groups (n＝5). Individual groups were superovulated with 100, 200 or 300 mg of Folltropin per animal. The superovulation treatment was initiated at Day 10 or Day 11 of the estrous cycle (Day 0＝day of estrus). Alfaprostol (6 mg) was injected to each cow 72 h after the initiation of superovulation treatment to induce eestrus. After confirming standing estrus, the cows were inseminated 2～3 times, 12 h apart, depending on the duration of estrus. At Day 6 or Day 7, individual horns of the uterus were flushed with 150～200 $m\ell$ of phosphate buffered saline supplemented with BSA (0.2％), penicillin (100 IU／$m\ell$) and streptomycin (100 $\mu\textrm{g}$／$m\ell$) using a two-way foley catheter. The embryos were concentrated, removing the excess medium through an embryo filter, and identified under a stereomicroscope. The identified embryos were collected, washed four times, evaluated and graded as excellent, good, fair or poor. The excellent, good and fair embryos were considered as transferable quality embryos. The mean (range). numbers of embryos collected vs. transferable quality embryos far 100, 200 and 300 mg of Folltropin were 4.5 (1～10) vs. 3.5 (1～8); 2.5 (1～4) vs. 1 (0～2) and 0.0 (0～0) vs. 0.0 (0～0), respectively, Folltropin at a dose of 100 or 200 mg produced suitable ovarian stimulation for superovulation in indigenous zebu cows of Bangladesh. A dose of 300 mg or more Folltropin consistently caused preovulatory corpora lutea formation in the ovaries and resulted in zero embryo recovery.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.427-433
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• 1992

Reproduction and growth to weaning were compared for Brahman crossbred (BX) and a local strain of South-East Asian cattle, "Javanese Zebu" (JZ) and their reciprocal crosses at "Erap" in the humid equatorial lowlands of Papua New Guinea. Forty heifers of each breed were mated continuously, half to bulls of each breed, for five years. BX calved first at 35 months while JZ calved at 31 months. Subsequent calving intervals were very short, at 370 and 341 days. JZ cows weighed about two thirds of the BX cattle at each stage of reproduction. Birth weights and growth to weaning were : BX 35 kg and 0.68 kg/d ; BX male $\times$ JZ female 29.3 kg and 0.53 kg/d ; JZ male $\times$ BX female 30.8 kg and 0.61 kg/d ; JZ 25 kg and 0.50 kg/d. The combination of small cow size, short calving interval and rapid calf growth resulted in the BX male $\times$ JZ female being the most efficient producer, in kg of calf weaned per cow mated per year while the reciprocal cross was the least efficient ; both straight-breds were equal and intermediate. These data show that indigenous equatorial cattle may not be inferior under good grazing conditions. For all traits, breed interactions (heterosis) was small and non-significant.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.6
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• pp.774-780
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• 2000

For this study three types of block such as medicated urea-molasses-mineral block (MUMMB, 7% urea), urea-molasses-mineral blocks ($UMMB_a$ 7% urea) and urea-molasses-mineral blocks ($UMMB_b$ 21% urea) were prepared. Naturally infested 24 indigenous dairy cows with gastrointestinal nematodes were used to study the effect of MUMMB, $UMMB_a$ and $UMMB_b$ on different parameters (body weight, milk yield, serum urea level, milk urea level). The cows were offered normal diet added with 250 g block per cow for every day. Straw and green grasses were used as basal diet in the ration. The managemental facilities for all cows of each group were similar. Cows were stall fed, calves were tied up at night and had free access to their dam during day time. Milking was done once daily in the morning. The daily average live weight gains by MUMMB, $UMMB_a$, $UMMB_b$, and control group (no block) were 52.08 g, 44.44 g, 48.58 g, and 1.41 g respectively. The live weight gain were statistically significant (p<0.01) in comparison to control group. The highest daily milk yield was observed in MUMMB treated cows (2.39 L/d); followed by $UMMB_b$ treated cows (1.82 L/d) and $UMMB_a$ treated cows (2.16 L/d). The lowest milk yield was found in control group (1.54 L/d). This result differs significantly (p<0.05) between treated groups and also with control group. During urea-molasses-mineral blocks $UMMB_a$, $UMMB_b$ licking milk urea level increased from initial $22.76{\pm}2.35mg/dl$ to $35.46{\pm}4.80mg/dl$ and initial $22.86{\pm}2.96mg/dl$ to $40.66{\pm}0.87mg/dl$ respectively. This variation of milk urea level was statistically significant (p<0.001). Similarly during $UMMB_a$, and $UMMB_b$ blocks licking, serum urea level increased in both treated groups from initial $22.76{\pm}2.60mg/dl$ to $32.68{\pm}2.21$ and initial $23.70{\pm}2.78mg/dl$ to $40.48{\pm}3.24mg/dl$, respectively. This variation of serum urea level was also statistically significant (p<0.001). Use of MUMMB instead of UMMB was proved better for milk production and live weight gain in dairy cows under the village condition of Bangladesh where balanced ration for dairy cows are a major scarcity. And various concentration of urea in blocks positively affect milk and serum urea levels of indigenous dairy cows.