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Whole genome sequencing of Luxi Black Head sheep for screening selection signatures associated with important traits

  • Liu, Zhaohua;Tan, Xiuwen;Wang, Jianying;Jin, Qing;Meng, Xianfeng;Cai, Zhongfeng;Cui, Xukui;Wang, Ke
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1340-1350
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    • 2022
  • Objective: Luxi Black Head sheep (LBH) is the first crossbreed specialized for meat production and was developed by crossbreeding Black Head Dorper sheep (DP) and Small Tailed Han sheep (STH) in the farming areas of northern China. Research on the genomic variations and selection signatures of LBH caused by continuous artificial selection is of great significance for identifying the genetic mechanisms of important traits of sheep and for the continuous breeding of LBH. Methods: We explored the genetic relationships of LBH, DP, and several Mongolian sheep breeds by constructing phylogenetic tree, principal component analysis and linkage disequilibrium analysis. In addition, we analysed 29 whole genomes of sheep. The genome-wide selection signatures have been scanned with four methods: heterozygosity (HP), fixation index (FST), cross-population extended haplotype homozygosity (XP-EHH) and the nucleotide diversity (𝜃π) ratio. Results: The genetic relationships analysis showed that LBH appeared to be an independent cluster closer to DP. The candidate signatures of positive selection in sheep genome revealed candidate genes for developmental process (HoxA gene cluster, BCL2L11, TSHR), immunity (CXCL6, CXCL1, SKAP2, PTK6, MST1R), growth (PDGFD, FGF18, SRF, SOCS2), and reproduction (BCAS3, TRIM24, ASTL, FNDC3A). Moreover, two signalling pathways closely related to reproduction, the thyroid hormone signalling pathway and the oxytocin signalling pathway, were detected. Conclusion: The selective sweep analysis of LBH genome revealed candidate genes and signalling pathways associated with developmental process, immunity, growth, and reproduction. Our findings provide a valuable resource for sheep breeding and insight into the mechanisms of artificial selection.

A Study on Efficient Sidewalk Green Space Development for Improving Urban Thermal Environments and Enhancing Pedestrian Comfort - Focused on the Derivation of Parameters for Design, and Proposing Methods for Green Space Creation - (도시열환경 개선 및 보행자 열 쾌적성 증대를 위한 효율적 가로녹지 조성방안 연구 - 계획 및 설계를 위한 매개변수 도출과 녹지조성 방법 제안을 중심으로 -)

  • Park, Ju-Hyeon;Eum, Jeong-Hee
    • Journal of the Korean Institute of Landscape Architecture
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    • v.52 no.2
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    • pp.21-38
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    • 2024
  • This study aims to establish an efficient street green area to improve the urban thermal environment and enhance pedestrian thermal comfort. Specifically, This study identified parameters applicable to green space planning and design, analyzed thermal environment mitigation mechanisms for each parameter, and, based on these findings, proposed methods for tree species selection and planting in green space planning and design. To achieve this, 61 papers were selected through a four-stage process from both domestic and foreign sources. The selected papers were analyzed, and the following main results were derived: In open street canyons with high stress levels due to low aspect ratios and high sky view factors(SVF), broadleaf trees with wide crown widths, low trunk heights, high leaf area index(LAI), and high crown heights were found effective in reducing heat, thereby increasing the amount and quality of shade. In contrast, in deep and narrow street canyons with relatively low heat stress due to high aspect ratios and low SVF, broad-leaved trees with narrow crown widths, high trunks, low crown heights, and low LAI were effective in reducing heat by enhancing ventilation. This study can serve as fundamental data for establishing standards for street green spaces to improve the thermal environment of street canyons and enhance thermal comfort of pedestrians. Additionally, it can be valuable when selecting the location and prioritizing street green spaces. Moreover, it is anticipated to be a foundational resource for creating guidelines for green space planning and design in response to climate change.

Relationship between Aboveground Biomass and Measures of Structure and Species Diversity in Quercus mongolica-Dominated Forest, Mt. Jeombong (점봉산 신갈나무군락의 생물종 다양성, 구조 다양성과 지상부 생물량의 관계에 대한 연구)

  • Jeong, Heon Mo;Jang, Inyoung;Hong, Seungbum
    • Korean Journal of Environment and Ecology
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    • v.30 no.6
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    • pp.1022-1031
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    • 2016
  • Relationships of standing biomass with biodiversity and structural diversity were examined in the Quercus mongolica-dominated forest in Mt. Jeombong, Gangwon-do. We examined the standing biomass of the Q. mongolia community ($311.1ton{\cdot}ha^{-1}$) from 2004 to 2013, and the observed major species were Q. mongoilca, Carpinus cordata, Tilia amurensis whose standing biomasses were $206.3ton{\cdot}ha^{-1}$ (66.3%), $36.9ton{\cdot}ha^{-1}$ (11.9%), and $30.6ton{\cdot}ha^{-1}$ (9.8%), respectively. Although the number of Q. mongolica individuals was very small compared with total density, the reason that Q. mongolica showed the most biomass than other species is due to greater average diameter at breast height (DBH) and the higher number of $DBH{\geq}50cm$ individuals. We calculated the range of Shannon index (H') and Shannon evenness (J') in the Q. mongolica community, and they were gradually increased in time, showing 2.015~2.166, 0.673~0.736, respectively. Their H' and J' showed positive linear relationships with their standing biomass. This indicates that the spatial distribution of the standing biomass in Q. mongoilca community becomes more homogeneous with time and this homogenization appears in various species in the community. In addition, we estimated biomass-species index (BS) and abundance-biomass-speciesdiversity (ABS) and they also showed gradual increase in time, ranging from 3.746 to 3.811 and from 4.781 to 5.028, respectively. Their indices showed positive linear relationships with the standing biomass. This can be explained from the observations of variations in standing biomass with tree diameters as the differences in the average standing biomass in the community have reduced gradually in time. Moreover, it is expected that increase in the structure diversity of the Q. mongoilca community enhances the efficiency in carbon sequestration and productivity, so the community can be developed to a more sustainable ecosystem with more abundant resources. Thus, applications of uneven-aged plantations with considerations of local ecological properties can be a very efficient reforestation method to ensure stable support of biodiversity and productivity.

Hierarchical Overlapping Clustering to Detect Complex Concepts (중복을 허용한 계층적 클러스터링에 의한 복합 개념 탐지 방법)

  • Hong, Su-Jeong;Choi, Joong-Min
    • Journal of Intelligence and Information Systems
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    • v.17 no.1
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    • pp.111-125
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    • 2011
  • Clustering is a process of grouping similar or relevant documents into a cluster and assigning a meaningful concept to the cluster. By this process, clustering facilitates fast and correct search for the relevant documents by narrowing down the range of searching only to the collection of documents belonging to related clusters. For effective clustering, techniques are required for identifying similar documents and grouping them into a cluster, and discovering a concept that is most relevant to the cluster. One of the problems often appearing in this context is the detection of a complex concept that overlaps with several simple concepts at the same hierarchical level. Previous clustering methods were unable to identify and represent a complex concept that belongs to several different clusters at the same level in the concept hierarchy, and also could not validate the semantic hierarchical relationship between a complex concept and each of simple concepts. In order to solve these problems, this paper proposes a new clustering method that identifies and represents complex concepts efficiently. We developed the Hierarchical Overlapping Clustering (HOC) algorithm that modified the traditional Agglomerative Hierarchical Clustering algorithm to allow overlapped clusters at the same level in the concept hierarchy. The HOC algorithm represents the clustering result not by a tree but by a lattice to detect complex concepts. We developed a system that employs the HOC algorithm to carry out the goal of complex concept detection. This system operates in three phases; 1) the preprocessing of documents, 2) the clustering using the HOC algorithm, and 3) the validation of semantic hierarchical relationships among the concepts in the lattice obtained as a result of clustering. The preprocessing phase represents the documents as x-y coordinate values in a 2-dimensional space by considering the weights of terms appearing in the documents. First, it goes through some refinement process by applying stopwords removal and stemming to extract index terms. Then, each index term is assigned a TF-IDF weight value and the x-y coordinate value for each document is determined by combining the TF-IDF values of the terms in it. The clustering phase uses the HOC algorithm in which the similarity between the documents is calculated by applying the Euclidean distance method. Initially, a cluster is generated for each document by grouping those documents that are closest to it. Then, the distance between any two clusters is measured, grouping the closest clusters as a new cluster. This process is repeated until the root cluster is generated. In the validation phase, the feature selection method is applied to validate the appropriateness of the cluster concepts built by the HOC algorithm to see if they have meaningful hierarchical relationships. Feature selection is a method of extracting key features from a document by identifying and assigning weight values to important and representative terms in the document. In order to correctly select key features, a method is needed to determine how each term contributes to the class of the document. Among several methods achieving this goal, this paper adopted the $x^2$�� statistics, which measures the dependency degree of a term t to a class c, and represents the relationship between t and c by a numerical value. To demonstrate the effectiveness of the HOC algorithm, a series of performance evaluation is carried out by using a well-known Reuter-21578 news collection. The result of performance evaluation showed that the HOC algorithm greatly contributes to detecting and producing complex concepts by generating the concept hierarchy in a lattice structure.

Characteristics of Seedling Quality of Daphniphyllum macropodum 2-year-old Container Seedlings by Shading Level (굴거리나무 2년생 용기묘의 피음수준별 묘목품질 특성)

  • Song, Ki Seon;Choi, Kyu Seong;Sung, Hwan In;Jeon, Kwon Seok;An, Kyoung Jin;Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.104 no.3
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    • pp.390-396
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    • 2015
  • This study was carried out in order to closely examine quality index by shading level of 2-year-old (1-1 seedling) container seedling of Daphniphyllum macropodum which is known as the species of having shade tolerance that is evergreen broad leaved tree in the warm temperate region. The shading treatment was regulated with the shading level of full sunlight, and 35%, 55%, 75%, 95% of full sunlight. As a result of surveying growth according to the shading level, both height and root collar diameter were surveyed to be the highest with 45.1 cm and 8.22 mm, respectively, under 75% of shading. The next was surveyed to be 43.2 cm & 8.05 mm and 42.5 cm & 7.98 mm, respectively, in order of 35% and 55% in shading. Leaf, shoot, root, and whole dry mass production were the highest under 75% of shading. The next was higher in leaf, stem, and whole dry mass production under 55% of shading. A root was higher under 35% of shading in the next. H/D ratio was the range of 5.29~5.35 under the 35~75% shading that showed the relatively high height and root collar diameter. T/R ratio was the lowest with 1.17 under 35% of shading. It was 0.41 under 95% of shading as for LWR, 0.24 under 75%-95% of shading as for SWR, and 0.46 under full sun and 35% shading as for RWR. QI was the highest with 3.74 under 75% of shading. As a result of surveying the whole experiment, it is concluded that the production of D. macropodum seedling is more effective under 75% shading

Estimation of Timber Production by Thinning Scenarios Using a Forest Stand Yield Model (임분 수확예측 모델을 이용한 간벌 시나리오별 목재수확량 예측)

  • Kim, Young-Hwan;Kim, Tae-Wook;Won, Hyun-Kyu;Lee, Kyeong-Hak;Shin, Man Yong
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.592-598
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    • 2012
  • Forest stand yield and its changes along with 10 thinning scenarios were estimated using a forest stand yield model for six major tree species in Korea, such as Pinus densiflora in Gangwon province, Pinus densiflora in other regions, Pinus koraiensis, Larix leptolepis, Quercus acutissima Carruth, Quercus mongolica. The 10 thinning scenarios were generated based on a number of constraints and assumptions. For instance, it was assumed that thinning is implemented between 15 years and 40 years with 5 year period and its duration should be at least 10 years. Also, the overall removal rate from the thinning treatments was assumed to be not greater than 60%. Under the 10 scenarios, the overall stand yield volumes from thinning and final harvesting were estimated for each species and site index. The results showed that highest yield volumes were obtained for Pinus densiflora in Gangwon province, Pinus koraiensis and Quercus mongolica when 30% of basal areas were thinned at 20 and 40 years, while highest yield volumes were obtained for Pinus densiflora in other regions and Larix leptolepis when 20% of basal areas were thinned at 20, 30 and 40 years. Those two scenarios gave the same amount of highest yield volume for Quercus acutissima Carruth. Also the results indicated that thinning treatment is effective to increase overall stand yield volume and its effects are larger with a higher site index. The largest thinning effects were found in Pinus densiflora in Gangwon province (28%) and Larix leptolepis (25%), while limited in Pinus koraiensis (12%). The forest stand yield model, used in this research, could be an effective tool for estimating the stand dynamics with various thinning treatments, but it could be improved in a further research that validates its applicability in the field.

The Variation of Natural Population of Pinus densiflora S. et Z. in Korea -Characteristics of Needle and Wood of Wangsan, Bonghwa and Yangju Populations- (소나무 천연집단(天然集團)의 변이(變移)에 관(關)한 연구(硏究)(VII) -왕산(旺山), 봉화(奉化), 양주집단(楊州集團)의 침엽(針葉) 및 재질형질(材質形質)-)

  • Yim, Kyong Bin;Lee, Kyong Jae
    • Journal of Korean Society of Forest Science
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    • v.40 no.1
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    • pp.1-18
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    • 1978
  • Three Pinus densiflora populations as shown in location map (Fig. 1) were studied in 1977. These succeed the population numbers 10, 11 and 12 after the preceeding populations. Following the previous study methods, 20 trees were chosen from each population and the morphological characteristics such as tree forms, branching habit, needle and wood properties were investigated. The results are summerized as follows; 1. The mean stand ages were ranged from 40 to 45. The growth performances of trees of population 10 and 11 was similar, but 12 seemed to be inferior more or less. 2. The ratios of clear bole length was 0.53 in population 12 as the highest but 0.43 for population 10 as the lowest. 3. The population 12 was considered to be a stand of the coarser branching habit having the crown index (The maximum crown diameter/the crown length) 1.65 though the mean branching angle indicates almost horizontal. 4. The differences were observed in the clear bole length ratios and crown-indices between populations as shown in Fig. 3 and 4. 5. No inter-population differences in serration density of needle was shown but significant inter and intra-population and individual differences (within population) in number of stomata rows and resin duct. 6. Population 12 shown 0.119 of resin duct index as the maximum. 7. The pattern of diameter growth, analyses based on the width of 10-year-ring segment unit (for example, the 1st segment denotes the width between pith center and 10th year ring and the 2nd one is from 11th to 20th year ring and so on.), was alike among populations as shown in Fig. 9. 8. No significant differences between population in mean summer wood percentages as well as in wood specific gravity was observed. The values of wood specific gravity were increased with the increase of ages in population 10 and 11 however vice versa in population 12. 9. The fiber length was increased with the increase of age but no differences between populations as shown in Fig. 12.

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Geographic Variation of Seed Characteristics and 1-year-old Seedling Growth of Zelkova serrata (느티나무 종자특성의 지리적 변이와 1년생 유묘의 생장)

  • Kim, In Sik;Lee, Joo Hwan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.15 no.4
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    • pp.234-244
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    • 2013
  • This study was conducted to determine the factors affecting to seed characteristics and seedling growth of Zelkova serrata. The seeds were collected from sixteen populations of Z. serrata and the seed characteristics (i.e., seed length, width, weight and full seed rate) were measured. We also measured the 1-year-old seedling growth of each population at nursery. All seed characteristics showed significant differences in population level. Seed length and seed width were positively correlated with latitude, longitude, altitude and distance from coast line of sampling site. Seed length and seed width also negatively correlated with annual mean temperature, mean temperature of growing season (Mar.~Oct.). The ratio of seed length/seed width showed inverse trend in case of seed length and seed width. Seed weight/1,000 grains had no correlation with geographic factors but showed negative correlation with annual mean dryness index. Seed weight/L showed negative correlation with latitude and longitude and positive correlation with mean temperature of growing season of sampling site. Full seed rate showed negative correlation with latitude, longitude and annual mean dryness index of sampling site. There were significant differences among populations, among family within population and among individuals within family in seedling growth. Height and diameter of root collar of seedling showed negative correlation with longitude and mean humidity of growing season of sampling site. Height growth of seedlings was not correlated with any seed characteristics but, diameter at root collar showed low negative correlation with seed weight/1,000 grains and seed weight/L. We discussed the implications of the results in view of tree improvement of Z. serrata.

Change of the Vegetation Due to Soyanggang Dam Construction (소양강댐 건설에 따른 주변 식생의 변화)

  • Choi, Ho;Park, Pil-Sun;Kim, Jae-Geun;Suh, Sim-Eun
    • Journal of Wetlands Research
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    • v.12 no.3
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    • pp.1-13
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    • 2010
  • Most of investigations about the effects of dam construction on the surrounding environments have focused mainly on the change of climate conditions and crop production. In order to research the effect of dam construction on the surrounding vegetation, we chose the Soyanggang dam whose storage capacity is the largest in Korea, and was built 33 years ago. We surveyed and analyzed the surrounding vegetation by using quadrat method and measured the soil moisture content among floodplain (FP), 5m above the flood plain (AFP) and control group (CG) which is 3km far from the lake through ridge. The largest value of mean importance percentage of the canopy~understory layer at FP was Salix koreensis (87.9%) and those of AFP and CG was Quercus mongolica (38.9% and 40.4% respectively) and the largest important percentage of the herb layer at FP was Artemisia capillaris (34.2%) and those of AFP and CG was Oplismenus undulatifolius var. undulatifolius (9.4% and 24.6% respectively). The Shannon-Wiener diversity index of shrub~canopy layer at FP (0.26) was lower than AFP (2.34) and CG (2.23) and there was not any significant difference in the herb layer among three groups. The S${\o}$rensen similarity index between FP and AFP, FP and CG was 0, and that of AFP and CG was relatively high. The highest density of tree and subtree with the DBH level of FP was S. koreensis of 5~10cm (240/ha), and that of AFP and CG was Quercus spp. of 15~20cm (400/ha and 466/ha respectively). And the highest density of seedlings of FP was Pinus densiflora (7,040/ha), and that of AFP and CG was Quercus spp. (720/ha and 400/ha respectively). The soil water content of FP (6.28%) was relatively lower than AFP and CG (11.13% and 10.14% respectively; p<.01). These results indicated that construction of Soyanggang dam changed the vegetation of the floodplain, without showing a change in its upland areas.

Evaluation of Community Land Model version 3.5-Dynamic Global Vegetation Model over Deciduous Forest in Gwangneung, Korea (광릉 활엽수림에서 Community Land Model 3.5-Dynamic Global Vegetation Model의 평가)

  • Lim, Hee-Jeong;Lee, Young-Hee;Kwon, Hyo-Jung
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.12 no.2
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    • pp.95-106
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    • 2010
  • The performance of Community Land Model version 3.5 - Dynamic Global Vegetation Model (CLM-DGVM) was evaluated through a comparison with the observation over temperate deciduous forest in Gwangneung, Korea. Influence of plant phenology, composition of plant functional type, and climate variability on carbon exchanges was also examined through sensitivity test. To get equilibrium carbon storage, the model was run for 400 years driven by the observed atmospheric data at the deciduous forest of the year 2006. We run the model for 2006 with the equilibrium carbon storage at Gwangneung forest and compared the model output with the observation. A comparison of leaf area index (LAI) between the model and observation indicated that the simulated phenology poorly represented the timing of budburst, leaf-fall, and evolution of LAI. Senescence of the phenology was delayed about four weeks and the simulated maximum LAI (of 5.8 $m^2$ $m^{-2}$) was greater than the observed value (of 4.5 $m^2$ $m^{-2}$). The overestimated LAI contributed to overestimation of both gross primary productivity (GPP) and ecosystem respiration $(R_e)$ through increased photosynthesis and foliar autotropic respiration $(R_a)$, respectively. Despite the discrepancy between the simulated and observed LAI, the simulated tree carbon storage amounts were comparable with the reported values at the site. Change in plant phenology from the simulated to the observed reduced more than six weeks of the plant growth period, resulting in the decreased amount of GPP and $R_e$. These values, however, were still higher (~10% of GPP and 40% of $R_e$) than the observed values. The effect of change in plant functional type composition (from dominant temperate deciduous forest to the coexistence of temperate deciduous and needle leaf forests) on the estimated amount of GPP and $R_e$ was marginal. The influence of climate variability on carbon storage amounts was not significant. The simulated inter-annual variation of GPP and $R_e$ from 1994 to 2003 depended on annual mean air temperature and total radiation but not on precipitation. Other deficiencies of CLM3.5-DGVM have been discussed.