• 한방재활의학과학회지
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• 제18권2호
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• pp.97-109
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• 2008

Objectives : Effects of Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) on intellectual development and learning ability were investigated growth and intellectual impairment rat with insufficient nutrition diet. Methods : We divided male Sprague-Dawley rats into 4 groups(A, B, C, D). They were normal group(A), growth deficiency rat with insufficient nutrition diet group(B), growth deficiency rat with 0.1% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group(C) and growth deficiency rat with 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group(D). They were administered for 5 weeks. We measured body weight, serum growth hormone, insulin-like growth factor and thyroid stimulating hormone, RBC, concentration of Hb and PCV ratio, total WBC and its composition, the values of plasma glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) activities and morris water maze test in escape distance, escape time and escape speed. Results : 1. Body weight showed a tendency to increase in the Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups and 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group showed significantly different than control groups. 2. Serum growth hormone, insulin-like growth factor and thyroid stimulating hormone showed a tendency to increase in Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups, however these values showed no significantly different. 3. About the counts of RBC, 0.1% and 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed significantly different than control groups. Concentration of Hb was higher than control group in Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups. And 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group showed significantly different than control groups in PCV ratio. 4. The counts of total WBC and its composition showed no significantly different in all treatment groups. 5. The values of plasma glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) activities showed no significantly different in all treatment groups. 6. In the morris water maze test, in escape distance and escape speed, Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed no significantly different than control group. But Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed the increasing tendency. Conclusions : So Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) have an effect of promoting growth of rats and might be effect to treat various kinds of growth delay in children.

• 대한핵의학회지
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• 제36권6호
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• pp.344-356
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• 2002

목적: 적혈구 혈액 풀 SPECT는 높은 특이도로 인하여, 간의 대표적인 양성 종양인 혈관종의 진단에 널리 사용되어 왔지만 낮은 해상도가 이 검사의 단점 중 하나였다. 최근 들어 ordered subset expectation maximization (OSEM)이라는 기술이 임상 핵의학 분야에서 단층영상의 재구성에 도입되고 있는 바, 저자들은 간 혈관종을 대상으로 기존의 역투사방식과 새로운 수정된 반복영상구성법인 OSEM을 비교하고자 하였다. 대상 및 방법: 24명의 간 혈관종 환자의 28개의 병변들 각각으로부터 이중 헤드 감마 카메라를 이용하여 단층영상 재구성을 위한 64개의 투사 영상을 얻었다. 이들 raw data는 LINUX운영체계의 개인용 컴퓨터에 보내서, 각각의 header file을 interfile로 대체하여 OSEM프로그램이 인식할 수 있도록 하였다. 최상의 영상을 구성하는 조건을 알아보기 위하여 다양한 subset 수(1, 2, 4, 8, 16 그리고 32) 및 반복계산 수 (1, 2, 4, 8, 그리고 16)하에서 재구성을 시도하여 4번의 반복계산과 16개의 subset일 때를 최적 조건으로 선택하였다. 이후 이 조건 하에서 OSEM과 역투사 방법으로 각각 모든 대상을 재구성한 후에 3명의 핵의학 및 방사선과 전문의가 특별한 정보 없이 모든 영상을 검토하였다. 결과: 28개의 병변을 맹검한 결과, 거의 모든 증례에서 OSEM이 역투사에 비교하여 최소한 대등하거나 우수한 영상의 질을 보여주었다. 비록 3 cm 이상의 큰 병변의 검출에는 차이가 없었으나 1.5-3 cm 크기의 병변 5개는 OSEM을 통하여서만 발견되었다. 하지만 1.5 cm 미만의 작은 병변 4개는 양쪽 모두에서 검출되지 않았다. 결론: OSEM은 작은 크기의 간 혈관종을 발견하는데 보다 높은 민감도를 보였으며 전체적인 영상의 질에 있어서도 보다 좋은 대조도와 윤곽을 보여주었다. OSEM은 이와 같은 장점 뿐만 아니라 높은 사양의 컴퓨터를 요하지않고 계산시간이 길지 않기 때문에 임상에서 간 혈관종의 진단을 위한 적혈구 혈액풀 SPECT에 쉽게 적용될 수 있는 좋은 방법으로 사료된다.

• 대한한방부인과학회지
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• 제19권2호
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• pp.107-126
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• 2006

Purpose : This study was peformed to evaluate the effect of Kamijoaguiem(JGE) on the bone mass and its related factors. Methods : We used ovariectomized rat as an estrogen-deficient animal model. The model rats of osteoporosis showed a significant decrease in bone density, bone ash density, calcium content of femur bone. At the 7th day after operating ovariectomy, rats were administered with JGE per orally, and continued for 10 weeks. And osteoporosis related parameters were determined to investigate the effect of JGE. Results : Bone density, bone ash density, bone calcium, magnesium and phosphorus was decreased in osteoporotic rats. JGE improved the decreased bone density, bone ash density and the decreased bone magnesium, but JGE didn't improve the decreased bone calcium and phosphorus in osteoporotic rats. Osteocalcin in serum and hydroxy-proline excretion in urine were increased in osteoporotic rats. Their levels were decreased when JGE was administered. ALP activity in serum was increased in osteoporotic rats. JGE didn't induce any significant changes. JGE showed significant increase in serum calcium level, total protein level, albumin level, BUN level, serum LDH activity. JGE didn't show significant increase in serum T-cholesterol density, triglyceride density, HDL-cholesterol density. JGE didn't show significant increase in RBC number, hemoglobin level, platelet number, hematocrit level. JGE showed inhibitory effect on the degradation of bone-matrix in osteoporotic rats, in histological examination to Hematoxylin-eosin stain. Conclusion : JGE might improve bone density due to inhibition of bone resolution in osteoporotic rats. It suggest that JGE may be useful prescription in osteoporosis.

• Clinical and Experimental Pediatrics
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• 제52권2호
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• pp.167-175
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• 2009

목 적 : 소아를 대상으로 생화학적 영양지표들이 각각의 체질량지수군 간에서 어떤 차이를 보이는 지 분석하고, 영양상태의 평가로 생화학적 영양지표들이 유용한 것인가를 알아보기 위하여 이 연구를 시행하였다. 방 법 : 2006년 1월부터 2007년 12월까지 강릉 아산병원에 선택적 수술을 받기 위해 내원한 3세 이상 10세 미만의 소아 중 신체계측 자료와 수술 전 혈액검사 자료가 모두 있는 269명을 대상으로 하였다. 체질량지수에 따라 저체중군, 정상체중군, 과체중군, 비만군으로 나누고 각 군 간에 TLC, 혈색소, 적혈구용적, 혈청 알부민, 총콜레스테롤 등과 같은 생화학적 영양지표가 의미있는 차이를 보이는 지를 후향적으로 분석하였다. 결 과 : 모든 체질량지수군 간에서 TLC는 유의한 차이가 없었다. 하지만 혈청 알부민은 저체중군에서 유의하게 감소되어 있었다. 적혈구수, 혈색소, 적혈구용적과 혈청 총콜레스테롤은 과체중군 또는 비만군에서 정상체중군보다 유의한 증가를 보였다. MCH와 MCV는 모든 군에서 유의한 증가가 없어, 과체중군과 비만군의 혈색소 및 적혈구수 등의 증가는 철분대사 보다는 적혈구 조혈기능의 증강 때문으로 생각된다. 남자와 여자에서 유의한 생화학적 영양지표가 서로 다르게 나타났는데 남자에서는 네 체질량군 간에 적혈구수, 혈색소, 적혈구 용적, 혈청 총콜레스테롤이 일부 유의한 차이를 보인 반면 여자에서는 유의한 차이를 보이지 않았고 알부민만 저체중군과 정상체중군 사이에서 유의한 감소를 보였다. 결 론 : 소아를 대상으로 시행한 생화학적 영양지표 중 적혈구수, 혈색소, 적혈구 용적, 혈청 알부민 및 총콜레스테롤은 체질량지수와 연관성이 있었다. 하지만 여자는 저체중군에서 알부민만 유의한 감소를 보였을 뿐 나머지 생화학 영양지표들과의 통계적 유의성은 없었다. 이에 대해 더 많은 연구가 필요할 것으로 생각된다.

• Nutritional Sciences
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• 제6권1호
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• pp.20-24
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• 2003

The effect of a-tocopherol on the formation and accumulation of phospholipid hydroperoxides, especially of phosphatidylcholine hydroperoxides, in the tissues of 2, 2 -azobis Hydrochloride (AAPH) - dosed rats was investigated. In a-tocopherol supplemented rats, the activities of glutathione peroxidase, catalase and superoxide dismutase were significantly inhibited, compared with the AAPH group. AAPH treatment led to oxidation of phospholipids in the liver, lungs, brain, plasma and red blood cells (RBC), resulting in a notable increase in phosphatidylcholine hydroperoxide (PCOOH). All tissues of the rats given an $\alpha$-tocopherol supplement showed an attenuation of the stimulating effect of AAPH, leading to low levels of formation of PCOOH. Also, the rats injected with AAPH and a-tocopherol showed relatively normal-appearing hepatocytes, except for a little loss of the granules. With regards to the morphological appearance of the liver, it was observed that oral intakes of a -tocopherol resulted in an antioxidant defense against attacks of peroxyl radicals. Thus, we suggest that a-tocopherol is potentially helpful in protecting membrane phospholipids against oxidative damage in vivo.

• 생약학회지
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• 제22권3호
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• pp.197-206
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• 1991

The water extract of Gamisamryungbaekchool-San increased the lifespan of mice implanted intraperitoneally with sarcoma 180. Significant depression of lethal toxicity of cis-platin $(45{\mu}M/kg,\;s.c)$ and renal toxicity (indicated by an increase in blood urea nitrogen value and creatinine value) of cis-platin $(35{\mu}M/kg,\;s.c)$ were observed in mice and rats treated with Gamisamryungbaekchool-San. RBC and WBC were significantly decreased in rats treated with cis-platin, and significant depression of hematologic toxicities of cis-platin $(35{\mu}M/kg,\;s.c)$ in rats treated with Gamisamryungbaekchool-San. After all, alleviative effect of the side actions of cis-platin was acknowledged by combined usage of Gamisamryungbaekchool-San and cis-platin.

• Applied Science and Convergence Technology
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• 제24권5호
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• pp.196-202
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• 2015

Human blood consists of 55% of plasma and 45% of blood cells such as white blood cell (WBC) and red blood cell (RBC). In plasma, there are many kinds of promising biomarkers, which can be used for the diagnosis of various diseases and biological analysis. For diagnostic tools such as a lab-on-a-chip (LOC), blood plasma separation is a fundamental step for accomplishing a high performance in the detection of a disease. Highly efficient separators can increase the sensitivity and selectivity of biosensors and reduce diagnostic time. In order to achieve a higher yield in blood plasma separation, we propose a novel fluid wing structure that is optimized by COMSOL simulations by varying the fluidic channel width and the angle of the bifurcation. The fluid wing structure is inspired by the inertial particle separator system in helicopters where sand particles are prevented from following the air flow to an engine. The structure is ameliorated in order to satisfy biological and fluidic requirements at the micro scale to achieve high plasma yield and separation efficiency. In this study, we fabricated the fluid wing structure for the efficient microfluidic blood plasma separation. The high plasma yield of 67% is achieved with a channel width of $20{\mu}m$ in the fabricated fluidic chip and the result was not affected by the angle of the bifurcation.

• 대한약침학회지
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• 제2권1호
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• pp.63-71
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• 1999

Counts of RBC and proportion of packed cell volume(PCV) showdc no difference among treatment groups. the values of hemoglobin in rat showed a tendeuy to increase in herbal-acupuncture group, however the values of hemoglobin in guineapig and rabbit showed no significant difference in the treatnent groups. the count of WBC showed high in the chishiI(BL52) herbal-acupuncture group. The proprtion of neutrophils showed no significant difference in rabbit, however the values in guinea-pig and rat showed a tendency to increased in herbal-acupuncture groups. The proportion of lymphocytes in rabbit and guinea-pig showed a tendency to decreast in herbal-acupuncture groups, however the values in rat showed no significant difference. The proportion of Eosinophils and monocytes showed no significant difference in all the treated groups. Results from this study indicated that shinsu(BL23) and chishil(BL52) herbal-acupuncture with palmijihwangtang elivate the biglogical function in Rabbit Guinea-pig and Rat.

• 대한의용생체공학회:의공학회지
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• 제31권2호
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• pp.99-105
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• 2010

In order to obtain velocity profile of blood flow with high spatial resolution, a micro PIV technique consisted of a fluorescent microscope, double-pulsed YAG laser, cooled CCD camera was applied to in-vitro blood flow experiment through a micro round tube of a diameter $100{\mu}m$. Velocity distributions of blood flow for rabbit were obtained. The viscosity profiles for shear rate were found at flowing condition. To provide hemorheological characteristics of blood flow, the viscosities for shear rate were evaluated. The viscosity of blood also steeply increase by decreasing shear rate resulting in Non-Newtonian flow, especially in low shear rate region caused by RBC rheological properties. The results show typical characteristics of Non-Newtonian characteristics from the results of velocity profile and viscosity for blood flow. From the inflection points, cell free layer and two-phase flow consisted with plasma and suspensions including RBCs can be separated.

• ALGAE
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• 제31권4호
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• pp.303-315
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• 2016

Compsopogon specimens collected in China were examined based on morphology and DNA sequences. Five molecular markers from different genome compartments including rbcL, COI, 18S rDNA, psbA, and UPA were identified and used to construct a phylogenetic relationship. Phylogenetic analyses indicated that two different morphological types from China clustered into an independent clade with Compsopogon specimens when compared to other global samples. The Compsopogon clade exhibited robust support values, revealing the affiliation of the samples to Compsopogon caeruleus. Although the samples were distributed in a close geographical area, unexpected sequence divergences between the Chinese samples implied that they were introduced by different dispersal events and from varied origins. It was speculated that Compsopogon originated in North America, a portion of the Laurentia landmass situated in the Rodinia supercontinent at approximately 573.89-1,701.50 million years ago during the Proterozoic era.Although Compsopogonhad evolved for a rather long time, genetic conservation had limited its variability and rate of evolution, resulting in the current monospecific global distribution. Additional global specimens and sequence information were required to increase our understanding of the evolutionary history of this ancient red algal lineage.