• 미생물학회지
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• 제38권4호
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• pp.230-230
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• 2002

Class Ⅲ chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class Ⅲ chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5′flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for past-transnational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P chrysogenum and genus Aspergillus.

• 미생물학회지
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• 제38권4호
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• pp.255-255
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• 2002

For rapid and sensitive measurement of antiviral activities, application of a recombinant virus containing firefly luciferase gene was attempted. Recombinant human cytomegalovirus (HCMV) containing luciferase gene driven by HCMV late gene pp28 promoter (HCMV/pp28-luc) was used to test the antiviral activities of three known compounds and the result was compared with results from the conventional plaque assay for measuring the production of infectious viruses. When human fibroblast cells were infected with HCMV/pp28-luc, luciferase activity was observed at 2 days after infection and reached maximum at 6 days after infection, whereas the production of infectious virus was maximal at 4 days after infection. The antiviral activities of ganciclovir, acyclovir, and papaverine were measured in HFF cells infected with HCMV/PP28-luc and the luciferase activity was compared with the infectious virus titers. Luciferase activity decreased as the concentration of ganciclovir or papaverine increased, while there was a slight decrease in luciferase activity with acyclovir. The level of the decrease in Luciferase activity was comparable to the level of decrease in the production of infectious virus. Therefore, the antiviral assay using recombinant virus HCMV/pp28-luc resulted in sensitivity similar to the conventional plaque assay with a significant reduction in assay time.

• 적정기술학회지
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• 제6권2호
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• pp.146-150
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• 2020

4차 산업혁명에 의한 급변하는 기술 발전 과정에서 정보취약계층을 위한 보조공학기술은 종종 간과되고 있다. 본 논문에서는 차세대 보조공학기술로써 전자석 액추에이터를 적용한 촉각 디스플레이를 소개하고자 한다. 다중배열 구조의 촉각 디스플레이는 셀의 크기와 간격을 좁혀 직선과 곡선을 통해 그림, 지도, 그래픽 등을 출력한다. 이는 시각장애인들이 촉각으로 2차원 정보를 확인 할 수 있게 만들고, 기존 정보통신 기술(ICT)와 융합하여 더 많은 정보를 제공한다. STEM교육(과학, 기술, 공학, 수학)에 접근할 수 있는 교육환경을 제공할 뿐만 아니라 대중교통, 공공인프라 등 스마트시티의 다양한 분야에까지도 적용될 수 있다. 본 논문에서는 전자석 액추에이터의 연구 및 제작과정을 설명하고, 그 적용 사례를 중심으로 해당 기술이 함의하는 사회, 경제적 가치와 가능성에 대해 살펴보고자 한다.

• 한국토양비료학회지
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• 제42권5호
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• pp.371-378
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• 2009

본 시험은 벼 재배시 녹비작물로 녹비용 보리의 투입효과를 구명하여 호밀 대체 가능성을 검토코자 수행하였다. 호밀은 출수기, 녹비용 보리는 출수기, 출수 후 3일 및 출수 후 10일에 경운로타리 작업을 하고 담수시켜 부숙을 촉진한 다음 녹비작물 무시용구는 표준시비, 녹비작물 시용구는 토양검정시비 및 무시비 하여 동진 1호를 재배 후 토양물리화학성, 녹비작물 부숙정도, 생육 및 수량성을 검토한 결과는 다음과 같다. 녹비작물 투입시기별 생체중은 각각 10a당 2,715, 2,352, 2,867 kg이였고 건물중에 있어서 총질소 함량은 1.31, 1.46, 1.38%이었으며 탄질률은 33.4, 28.7, 34.6이였다. 녹비작물 투입으로 작토심이 깊어졌고 토양경도와 용적밀도가 낮아졌으며 공극률이 증가하여 물리성이 개선되었다. 또한 시험 전에 비하여 녹비용 보리 환원으로 양이온치환용량이 높아졌으며 치환성 양이온 함량이 증가되는 경향을 나타냈다. 쌀 수량은 관행($559kg\;10a^{-1}$)에 비하여 녹비용 보리+토양검정진단시비구에서 3~9% 증수되었으며, 현미 외관품위 중 완전립비율은 관행(73.0%)에 비하여 녹비용 보리 투입시 73.6~78.7%로 높은 경향을 나타냈다. 결론적으로 녹비용 보리를 출수 후 10일경 투입하여 벼 재배시 질소비료 절감과 지력개선에 효과적인 것으로 판단되었다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1991년도 하계학술대회 논문집
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• pp.162-165
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• 1991

Oxygen behaviors in CZ-silicon wafer, grown by the Lucky Advanced Materials Inc. that is a pioneer of silicon material industries in Korea, were investigated to simulate effects on the device performance of oxygen, neglecting the effect of other impurity content, defects and thermal history. Silicon wafers were annealed through simulated 16K SRAM thermal cycle. As initial oxygen concentration increased up to 16.7ppma the amount of oxygen precipitation increased up to 10.6ppma and the bulk microdefect density increased up to $10.3{\times}10^3/mm^2$, but the depth of the denuded zone decreased to $5.0{\mu}m$

• 생약학회지
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• 제5권2호
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• pp.103-109
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• 1974

미국인삼(美國人蔘) (오가과(五加科), Panax quinquefolium L.) 2년생(年生) 및 4년생(年生) 식물(植物)에 심지법(法)을 사용(使用)하여 $squalene-H^3$를 투여(投與)했을 때 미국인삼(美國人蔘) 사포닌(panaquilins)과 그 비당체(非糖禮)에 $H^3-incorporation$ 가능성(可能性) 여부(與否)를 검토 하였다. $squalene-H^3$ 섭취율은 매우 낮은 값을 보였으며 $(40{\sim}86%)$, $squalene-H^3$는 또한 panaquilin의 비당체(非糖體) 부분(部分)에는 incorporation 되지는 않았으나 당체(糖體)에는 squalene 대사물질(代謝物質)이 incorporation된 것으로 사료(思料) 되었다.

• 미생물학회지
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• 제28권4호
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• pp.318-323
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• 1990

The bacterial secondary production was measured at 6 sites of Lake Soyang in October, 1989 by $^{3}$H-thymidine incorporation rate. Verfication for the method of bacterial secondary production measurement showed that $^{3}$H-thymidine incorporated into DNA, RNA and protein by average percentage of 38.45, 42.27 and 20.07%, respectively. THe more increased incoporated $^{3}$H-thymidine, the more increasde DNA fraction, but protein fraction was generally low. Incorporation of rate of /usp 3/H-thymidine. $^{3}$H-leucine into protein correlated with protein fraction of incorporated $^{3}$H-thymidine. Conversion factors were calculated as follows; $1.83*10 ^{20}$ cells/moles of thymidine incorporated/hr and 1.69*10$^{22}$ cells/moles of leucine incorporated/hr.

• 대한치과의사협회지
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• 제19권7호통권146호
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• pp.609-614
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• 1981

Effects of steroid hormones on the collagen biosynthesis in aorta and uterus were studied with ovariectomized Sprague-Dawley rats. Effects of administration of hormones, such as estrogen, testosterone and prednisolone, to the ovariectomized animals were studied, comparing with the control. Each group was injected with ³H-proline and sacrificed, followed by removals of aorta and uterus. Separations and quantitative analyses of proline and hydroxyproline were performed by means of thin layer chromatography; and radioactivities of the separated amino acids were assayed by liquid scintillation counter. Normally the incorporation of ³H-proline into hydroxyproline was greater in uterus than in aorta, and collagen turnover rate of uterus was observed rapid as well than that of aorta. In the two tissues from ovariectomized rats, the incorporation rate of ³H-proline into hydroxypoline was markedly decreased than that of the former. Changes in the turnover rate of collagen in these tissues were not observed. Decrease in ³H-proline incorporation into collagen in ovariectomized rats was markedly antagonized by estrogen, but not influenced by prednisolone in the tissues tested.

• 한국진공학회지
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• 제5권4호
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• pp.354-358
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• 1996

precuror alternating metalorganic chemical vapor deposition(PAMOCVD)에 의한 InGaAs의 성장시에 높은 indium의 유입이 관찰되었다. gallium과 indium의 전구물질의 분해의 차이 그리고 이에따른 분해된 전구물질 분자의 흡착행동의 차이를 고려함으로써 PAMOCVD 성장시의 결정내로의 높은 indium유입의 mechanism을 제안하였다.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1087-1090
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• 2014

A simple and general method that selectively introduces metal binding sites into a protein can greatly increase the ability to design and biosynthesize artificial metalloproteins. Here, we report the incorporation of a phenanthroline-containing amino acid (Phen-Ala) into proteins in Escherichia coli by using the $tRNA{^{Tyr}}_{CUA}$ and tyrosyl aminoacyl-tRNA synthetase pair (BpyRS) from Methanococcus jannaschii, which was originally developed for a bipyridine-containing amino acid (Bpy-Ala). The incorporation efficiency of BpyRS for Phen-Ala was comparable to that for Bpy-Ala. Because of its high metal-binding ability and characteristic spectral properties, Phen-Ala can be a useful alternative to the existing metal-chelating amino acids for the design and synthesis of artificial metalloproteins.