• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.299-303
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• 2012

Inbreeding is the mating of relatives that produce progeny having more homozygous alleles than non-inbred animals. Inbreeding increases numbers of recessive alleles, which is often associated with decreased performance known as inbreeding depression. The magnitude of inbreeding depression depends on the level of inbreeding in the animal. Level of inbreeding is expressed by the inbreeding coefficient. One breeding goal in livestock is uniform productivity while maintaining acceptable inbreeding levels, especially keeping inbreeding less than 20%. However, in closed herds without the introduction of new genetic sources high levels of inbreeding over time are unavoidable. One method that increases selection response and minimizes inbreeding is selection of individuals by weighting estimated breeding values with average relationships among individuals. Optimum genetic contribution theory (OGC) uses relationships among individuals as weighting factors. The algorithm is as follows: i) Identify the individual having the best EBV; ii) Calculate average relationships ($\bar{r_j}$) between selected and candidates; iii) Select the individual having the best EBV adjusted for average relationships using the weighting factor k, $EBV^*=EBV_j(1-k\bar{{r}_j})$ Repeat process until the number of individuals selected equals number required. The objective of this study was to compare simulated results based on OGC selection under different conditions over 30 generations. Individuals (n = 110) were generated for the base population with pseudo random numbers of N~ (0, 3), ten were assumed male, and the remainder female. Each male was mated to ten females, and every female was assumed to have 5 progeny resulting in 500 individuals in the following generation. Results showed the OGC algorithm effectively controlled inbreeding and maintained consistent increases in selection response. Difference in breeding values between selection with OGC algorithm and by EBV only was 8%, however, rate of inbreeding was controlled by 47% after 20 generation. These results indicate that the OGC algorithm can be used effectively in long-term selection programs.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6035-6040
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• 2013

Background: Cancer is a complex disease caused by multiple factors, both genetic and environmental. It is a major health concern worldwide, in the Middle East and in Jordan specifically and the fourth most common killer in the Middle East. Hypothesis: The relative genetic homogeneity of the Circassian and Chechan populations in Jordan results in incidences of cancer that differ from the general Jordanian population, who are mostly Arabs. Materials and Methods: National Cancer Registry data were obtained for the years 1996-2005 The Chechen and Circassian cancer cases were identified and cancer registry data were divided into three populations. Crude rates were calculated based on the number of cancer cases and estimated populations. Results: Breast cancer is the most common cancer type constituting about one third of female cancers in all three populations. Higher crude rates are observed in the Circassian and Chechen populations than in the Arab Jordanian population. The rate ratios (95%CI) in Circassians and Chechens with respect to the Arab Jordanian population are 2.1 (1.48, 2.72) and 1.81 (1.16, 2.85), respectively. Lung cancer is the most common cancer in male Arab Jordanians and Chechens with crude rates of 4.2 and 8.0 per 100,000 respectively. The male to female ratio in these two populations in respective order are 5:1 and 7:1. The lung cancer crude rate in Circassians is 6.5 per 100,000 with a male to female ratio of only 1.6:1. The colorectal cancer crude rates in Arab Jordanians and Chechens are similar at 6.2 and 6.0 per 100,000, respectively, while that in Circassians is twice as high. Conclusions: Considerable ethnic variation exists for cancer incidence rates in Jordan. The included inbred and selected populations offer an ideal situation for investigating genetic factors involved in various cancer types.

• Interdisciplinary Bio Central
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• v.2 no.4
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• pp.14.1-14.9
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• 2010

Misidentification of cultured cell lines results in the generation of erroneous scientific data. Hence, it is very important to identify and eliminate cell lines with a different origin from that being claimed. Various methods, such as karyotyping and isozyme analysis, can be used to detect inter-species misidentification. However, these methods have proved of little value for identifying intra-species misidentification, and it will only be through the development and application of molecular biological approaches that this will become practical. Recently, the profiling of microsatellite variants has been validated as a means of detecting gene polymorphisms and has proved to be a simple and reliable method for identifying individual cell lines. Currently, the human cell lines provided by cell banks around the world are routinely authenticated by microsatellite polymorphism profiling. Unfortunately, this practice has not been widely adopted for mouse cells lines. Here we show that the profiling of microsatellite variants can be also applied to distinguish the commonly used mouse inbred strains and to determine the strain of origin of cultured cell lines. We found that approximately 4.2% of mouse cell lines have been misidentified; this is a similar rate of misidentification as detected in human cell lines. Although this approach cannot detect intra-strain misidentification, the profiling of microsatellite variants should be routinely carried out for all mouse cell lines to eliminate inter-strain misidentification.

• Asian-Australasian Journal of Animal Sciences
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• v.1 no.2
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• pp.73-79
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• 1988

Experiments were conducted to determine whether supplemental cobalt (Co) or nickel (Ni) would prevent the signs of zinc (Zn) deficiency in chicks fed a high calcium (1.5%) corn-soybean diet and to examine the difference in $^{65}Zn$ absorption rates between inbred Ancona and crossbred New Hampshire X Single Comb White Leghorn chicks. In the initial experiment, the supplementation of 27 ppm Ni, 27 ppm Co or 54 ppm Co to a basal diet increased weight gain and reduced feather defects; 54 ppm Ni tended to increase weight gain but did not reduce feather defects. In further experiments, chicks fed the diet supplemented with 54 ppm Co usually showed increased weight gain and reduced feather defects; however, chicks fed a diet supplemented with 54 ppm Ni less frequently showed these effects. In another test, Ancona chicks fed a diet supplemented with 30 ppm Zn (except during a $^{65}Zn$ absorption study period) showed lower weight gain, more feather defects and less $^{65}Zn$ absorption than did New Hampshire X Leghorn cross chicks. Similar results were achieved with two strains of chicks fed the basal and 54 ppm Ni, 54 ppm Co or 60 ppm Zn supplemented diets. The sparing effects of Co on Zn which were commonly observed and the lesser effect of Ni or Zn were shown to be, at least in part, the result of increased availability of dietary Zn. That Ancona chicks required more Zn than New Hampshire cross chicks for the development of feathers and for growth is partly the result of decreased Zn absorption from the type of diets fed.

• Development and Reproduction
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• v.17 no.1
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• pp.17-24
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• 2013

This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

• Korean Journal of Animal Reproduction
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• v.15 no.3
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• pp.179-187
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• 1991

This experiment was carried out to develop a new technique of identifying XX of XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wastar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentraton or affected H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. However, production of live young or sex rates of male and female from embryos transferred with psudopregnant. The biological test with the morula stage embryos showed that H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the bisected mouse embryos were cultured in vitro for 5~6 hours in morula stage, of 457 bisected embryos 81.4% of then were developed to the blastocyst stage. When the concentration rate of complement to H-Y antiserum varied from 1.0~5.0${mu}ell$, the lysis-rate of embryo was 19.5 to 67.3%. The concentration rate of complement did not influence the lysis-rate of embryos(P<0.05). The morphology embryos of bisected, zona-free and intact embryos showed the embryos lysis rate of 58.6, 42.7 and 48.5% respectively(P<0.05). Pregnancy rate were 50.0, 45.5 and 57.1% in psudopregnant recipient transferred with bisected, zona-free and intact blastocyst embryos. However, production of live youngs, sexual rate of male or female was 24(50.0:50.0), 22(45.5:55.5) and 36(58.3:41.7)mice, but affected and non affected half embryos with H-Y antiserum treatment was 23.1 and 26.7%. Also production of live youngs and sexual rate was 14(92.9:7.1) and 17(17.6:82.4)mice in affected and non affected half embryos in H-Y antiserum treatment(P<0.05).

• Korean Journal of Poultry Science
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• v.44 no.3
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• pp.199-209
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• 2017

Mitogen-activated protein kinase (MAPK) signaling pathways play a key role in innate immunity, inflammation, cell proliferation, cell differentiation, and cell death. The main objective of this study was to investigate the expression level of candidate MAPK pathway genes in the intestinal mucosal layer of two genetically disparate chicken lines (Marek's disease-resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE). Using high-throughput RNA sequencing, we investigated 178 MAPK signaling pathway related genes that were significantly and differentially expressed between the intestinal mucosal layers of the NE-afflicted and control chickens. In total, 15 MAPK pathway genes were further measured by quantitative real-time PCR(qRT-PCR) and the results were consistent with the RNA-sequencing data. All 178 identified genes were annotated through Gene Ontology and mapped onto the KEGG chicken MAPK signaling pathway. Several key genes of the MAPK pathway, ERK1/2, JNK1-3, p38 MAPK, MAP2K1-4, $NF-{\kappa}B1/2$, c-Fos, AP-1, Jun-D, and Jun, were differentially expressed in the two chicken lines. Therefore, we believe that RNA sequencing and qRT-PCR analysis provide resourceful information for future studies on MAPK signaling of genetically disparate chicken lines in response to pathogens.

• Plant Biotechnology Reports
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• v.4 no.1
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• pp.23-27
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• 2010

Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.249-256
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• 2007

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.626-631
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• 1997

This study was conducted to clarify the genetic effect on the duration of grain filling with using the eight corn inbreds. In diallel cross analysis, the grain filling during the lag period showed partial dominance with great additive effects. Inbreds FR14A and A508 showed greater recessive gene effects for lag period, while FR25 showed greater effects of dominant genes. The genetic analysis for the effective filling period(EFP) showed over dominance without additive gene effects. FR25 of 8 inbreds showed greatest effects of dominant genes for EFP, while YUBC208 showed greater recessive gene effects for EFP than other inbreds. The genetic analysis for total grain filling period(TGFP) seemed to be due to partial dominance with greater additive effects. Early inbred line, YUBC208 especially showed greater recessive gene effects for TGFP than others. No. of effective genes related to EFP and TGFP were estimated by at least 5.