• Journal of Microbiology and Biotechnology
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• 제31권8호
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• pp.1175-1182
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• 2021

We investigated the effect of bovine lactoferricin (Lfcin-B), a peptide derived from bovine lactoferrin, on activation of intestinal epithelial cells in IEC-6 intestinal cell, and protection against in vivo rotavirus (RV) infection. Treatment with Lfcin-B significantly enhanced the growth of IEC-6 cells and increased their capacity for attachment and spreading in culture plates. Also, Lfcin-B synergistically augmented the binding of IEC-6 cells to laminin, a component of the extracellular matrix (ECM). In the analysis of the intracellular mechanism related to Lfcin-B-induced activation of IEC-6 cells, this peptide upregulated tyrosine-dependent phosphorylation of focal adhesion kinase (FAK) and paxillin, which are intracellular proteins associated with cell adhesion, spreading, and signal transduction during cell activation. An experiment using synthetic peptides with various sequences of amino acids revealed that a sequence of 9 amino acids (FKCRRWQWR) corresponding to 17-25 of the N-terminus of Lfcin-B is responsible for the epithelial cell activation. In an in vivo experiment, treatment with Lfcin-B one day before RV infection effectively prevented RV-induced diarrhea and significantly reduced RV titers in the bowels of infected mice. These results suggest that Lfcin-B plays meaningful roles in the maintenance and repair of intestinal mucosal tissues, as well as in protecting against intestinal infection by RV. Collectively, Lfcin-B is a promising candidate with potential applications in drugs or functional foods beneficial for intestinal health and mucosal immunity.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.522-530
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• 2002

We have already known, neural progenitor cells exist not only in the developing brain, but in certain spots in adult CNS in mammals, so it will be of great value to find out some compounds which can interfere these cells proliferation ability. In this research, we observed that ginsenoside $Rg_1$ can not only enhance neural progenitors' proliferation ability in vitro, but increase neurogenesis in adult mouse dentate gyrus in vivo. Firstly, we set up neural progenitor cells' culture system from embryonic rats' hippocampus and prove their feature through immunocytochemistry. Then by using MTT assay, we found that when growing with ginsenoside $Rg_1(0.5\~2.5{\mu}mol/l)$, the progenitor cells' survival rate nearly doubled, furthermore, we proved that this increase was due to the increment of cell proliferation through $^3H-thimidine$ incorporation assay, hence, we drew the first conclusion: ginsenoside Rg1 has the ability to stimulate neural progenitor cells' proliferation in vitro; in order to observe this compound's effect in vivo, we devised the following experiment: after administering ginsenoside Rg1 (5, 10 mg/kg, once a day) intraperitoneally for two weeks, we examine the number of BrdU positive cells in the dentate gyrus of mice, and found that Rg1 could increase the number of proliferation cells significantly in vivo. From these studies, we are quite sure about Rg1's effects on the proliferation ability of neural progenitor cells both in vitro and in vivo, certain targets of the compound and its underlying mechanisms are in progress.

• 대한약침학회지
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• 제7권3호
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• pp.59-71
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• 2004

Objectives : In order to investigate effects and immune improvement of distilled white-ginseng herbal extract, expression of Cox-1, Cox-2, and mRNA of Bcl-2 and Bax were analyzed in A549 cell in vivo. Survival time and expression of cytokine mRNA were measured for the mice with Sarcoma-180 induced abdominal cancer. Methods : Balb/c mouse was treated with distilled white-ginseng Herbal Acupuncture at Wisu($Bl_{21}$) and Chung-wan($CV_{12}$) to investigate anti-cancer effects and immune response. Results : 1. For expression of mRNA of Cox-1 using RT-PCR. the control group and the experiment groups show significant increase. For Cox-2, both experiment groups and the normal group showed significant decrease. For Bcl-2, experiment groups showed slight decrease compared to the control group. For Bax, no significant changes were shown between the control group and experiment groups 2. For survival time, all of experiment groups didn't show significant differences. 3. IL-2 productivity using Flow cytometry, experiment group I didn't show any significance, For II-4, all of experiment groups showed slight decrease compared to the control group. 4. For IL-2 productivity using ELISA, experiment group I showed slight decrease compared to the control group, experiment group II didn't show any significance. 5. For expression of cytokine mRNA using RT-PCR, significant increase of IL-2 and IL-4 were witnessed in experiment group I compared to the control group. Significant decrease of IL-10 was shonwn in all of experiment groups compared to the control group. Conclusion : According to the results, we can expect that distilled white-ginseng Herbal Acupuncture may be further effects in anti-cancer and immune improvement if increasing concentration.

• 대한치과교정학회지
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• 제22권2호
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• pp.309-325
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• 1992

The purpose of this study was to evaluate the effect of intrinsic factor and extrinsic factor for growth of the mandibular condylar cartilage of 4 day-old rats in a serum-free medium for 1, 4, 7, 14 days. They were compared with normal growth in vivo and with growth of spheno-occipital synchondrosis in serum-free medium. The cellular kinetics of cartilages were evaluated by auto-radiography of tritiated thymidine. 1. Condylar cartilage was enlarged with rounded head on day 14 of experiment while in vivo the rounded-headed shape changed into functionally flattened appearance. 2. On day 14 of experiment, a severe reduction of the proliferative zone and a considerable increase of the hypertrophic zone were observed while in normal control group endochondrol bone formation and bone marrow were observed. 3. The proliferative activity in the proliferative zone of condylar cartilage detected by $^3H-thymidine$ incorporation was lower than that of normal control group and decreased more than that of spheno-occipital synchondrosis, but it continued during the 14 days of culture. 4. The continued maintenance of condylar cartilage and morphologic change were disturbed in this culture system, but cell division within the proliferative zone was continued and probably linked to intrinsic factor.

• Natural Product Sciences
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• 제20권3호
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• pp.196-201
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• 2014

Pyunkang-hwan (Pyunkang-tang) extract (PGT) is a traditional folk medicine for controlling diverse pulmonary diseases including bronchitis, tonsiltis and pneumonitis. We investigated whether PGT significantly affects secretion, production and gene expression of airway mucin using in vivo and in vitro experimental models reflecting the hypersecretion and/or hyperproduction of mucus observed in inflammatory pulmonary diseases. For in vivo experiment, effect of PGT was checked on hypersecretion of pulmonary mucin in sulfur dioxide-induced bronchitis in rats. For in vitro experiment, confluent NCI-H292 cells were pretreated with PGT for 30 min and then stimulated with EGF (epidermal growth factor), PMA (phorbol 12-myristate 13-acetate) or TNF-${\alpha}$ (tumor necrosis factor-${\alpha}$) for 24 h. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) PGT inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-${\alpha}$ from NCI-H292 cells, respectively; (2) PGT also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells, respectively; (3) PGT inhibited secretion of mucin in sulfur dioxide-induced bronchitis rat model. This result suggests that PGT can regulate secretion, production and gene expression of airway mucin.

• Preventive Nutrition and Food Science
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• 제5권1호
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• pp.42-47
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• 2000

For the development of functional food for hair-growth stimulation, this study has tried an in vivo and clinical test. As an in vivo test male Sprague Dawley rats and as a clinical test 27 baldheaded or loosehaired men were recruited. Before the experiment, the total hair count in 6 $\textrm{mm}^2$ of the designated area was 46.5. In four weeks and eight weeks of the functional food feeding it increased to 61.8 and 75.3, respectively. Hence the net increase was totaled at 62% in eight weeks. Also, depilation was decreased by 28%. Before the experiment, average hair loss was 65.7. In four weeks and eight weeks of the functional food feeding it decreased to 55.2 and 47.3, respectively. LDL and phospholipids were decreased by 42% and 36%, respectively during that period. However, HDL was increased by 21%. Forty percent of the subjects responded that itching of head skin was reduced and 34% responded to have reduced dandruff. No side-effects among the subjects were examined and no other blood pa-rameters were significantly affected by the diet. The results in this study suggest that biotin-rich functional food may stimulate cholesterol and lipid metabolism and blood flow leading to the growth of new hair and prevention of hair loss.

• Nuclear Engineering and Technology
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• 제55권6호
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• pp.2018-2025
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• 2023

In our previous study, we proposed an integrated PG-PET-based imaging method to increase the prediction accuracy for patient dose distributions. The purpose of the present study is to experimentally validate the feasibility of the PG-PET system. Based on the detector geometry optimized in the previous study, we constructed a dual-head PG-PET system consisting of a 16 × 16 GAGG scintillator and KETEK SiPM arrays, BaSO₄ reflectors, and an 8 × 8 parallel-hole tungsten collimator. The performance of this system as equipped with a proof of principle, we measured the PG and positron emission (PE) distributions from a 3 × 6 × 10 cm³ PMMA phantom for a 45 MeV proton beam. The measured depth was about 17 mm and the expected depth was 16 mm in the computation simulation under the same conditions as the measurements. In the comparison result, we can find a 1 mm difference between computation simulation and measurement. In this study, our results show the feasibility of the PG-PET system for in-vivo range verification. However, further study should be followed with the consideration of the typical measurement conditions in the clinic application.

• 한국한의학연구원논문집
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• 제16권2호
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• pp.83-90
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• 2010

Objective : The purpose of this study was to provide the information about Bojungikki-tang by domestic papers and theses. Method : Domestic papers related to Bojungikki-tang were reviewed and analyzed. These papers were classified by year, research method, experimental method, and subject. Result : The following results were obtained in this study. 1. The study of Bojungikki-tang started from 1984 and continued steadily. 2. The domestic papers about Bojungikki-tang were 47 volumes. Among them, there were 26 volumes since 2000. 3. Classified by research method from the year 2000, 20 experimental studies, 4 clinical experiments, and 2 documentary researches. 4. Classified by experimental method from the year 2000, 10 studies used mouse in vivo experiment and 4 studies used rat in vivo experiment and 6 studies used each cell in vitro experiment. 5. Classified by subject from the year 2000, papers related to immune enhancing effect, nerve and mental system, radiation protection effect, anti-allergy, anti-inflammatory, anti-cancer, remedy of diabetes, skin protection and so on. Conclusion : Many papers of experimental studies, clinical experiments and documentary researches related Bojungikki-tang are included in domestic journals. Bojungikki-tang is being researched variously in immune enhancing effect, nerve and mental system, radiation protection effect, anti-allergy, anti-inflammatory, anti-cancer, remedy of diabetes, skin protection and so on. However, more clinical studies on Bojungikki-tang are needed.

• 한국수정란이식학회지
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• 제26권3호
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• pp.195-199
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• 2011

포유동물 수정란의 동결보존기술은 최근 기후 변화에 따른 생물종 다양성을 보존하기 위해서 중요하게 여겨지는 연구 분야이다. 따라서 멸실 위험에 처한 동물의 개량과 증식, 보존과 복원 및 생명공학의 분야에 이르기까지 응용 기술은 다양하게 이용되어진다. 본 연구에서는 한우 수정란의 동결 후 생존성 향상을 위해서 동결 방법에 따른 체내 외수정한의 내동성을 조사하였다. 완만동결에 따른 체내 외수정란의 동결 융해 후 수정란의 재확장률은 89.6%와 81.5% 그리고 72시간 배양하였을 때 부화된 수정란은 76.9%와 43.4%, 생존수정란의 총세포수는 136${\pm}$3.6개와 107${\pm}$3.8개의 결과를 보여 동결 융해 후 생존율에서는 차이를 보이지 않았으나, 수정란의 부화율과 세포수 조사에서는 체내수정란이 유의적으로(p<0.05) 높은 결과를 보였다. 체내수정란의 완만동결 후 배반포 회복률은 91.3% 그리고 12시간 이상 배양하였을 때 확장배반포까지의 발달률은 71.4%의 결과를 보였으며, 초자화 동경에서는 85.7%와 75.0%의 결과를 보였다. 결과적으로 본 연구에서 수행된 체내 외 수정란의 동결기술을 이용한다면 수정란이식 현장에서도 효과적인 활용이 가능할 것으로 사료된다.

• 응용통계연구
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• 제6권2호
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• pp.383-391
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• 1993

암치료에 있어서 암세포의 약제 내성 극복에 관한 연구는 새로운 항암제의 개발 못지 않게 중요한 과제이다. 다약제 내성을 극복하기 위하여서는 여러 가지 변조물질(modulators)이 항암제와 결합투여되고 있지만, 아직까지 이들 변조물질중 어느 것이 다약제 내성의 극복에 더 효과적인지는 밝혀지지 않고 있다. 본 논문에서는 인체 암세포주를 대상으로 여러 종류의 항암제에다 변조물질들을 결합투여하는 경우 용량-반응곡선으로부터 상승효과를 검정할 수 있게 통계기법을 소개하고, 또한 일반화 선형모형의 계산 프로그램인 글림 패키지(GLIM Package)의 매크로(macro)를 사용하여 실제 실험자료를 분석한다. 이러한 분석결과는 in-vivo 실험을 통한 실제 임상적용의 근거를 제공하리라 믿어진다.