• 제목/요약/키워드: in vitro digestion

검색결과 226건 처리시간 0.028초

In Vitro Digestibility of Rice and Barley in Forms of Raw Flour and Cooked Kernels

  • Han, Jung-Ah;Jang, Su-Hae;Lim, Seung-Taik
    • Food Science and Biotechnology
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    • 제17권1호
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    • pp.180-183
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    • 2008
  • Digestion properties of 3 types of cereals, white rice, brown rice, and barley, were measured after cooking or grinding. Regardless of the processing methods, white rice showed the highest rate and the greatest extent of digestion, whereas barley showed the lowest values. During the early digestion period, cooked white rice kernels had a larger k (kinetic constant) value than uncooked white rice flour, indicating that cooking induced faster digestion than grinding. In the case of brown rice and barley, the cell wall in cooked kernels remained intact and resulted in a lower k values than those of uncooked flour. However, after 3 hr of digestion, the total digestion extent was greater for the cooked brown rice and barley than that for uncooked flours. The high content of slowly digestible starch (SDS) in cooked brown rice and barley might be due to the starch fraction which was protected by the cell wall. The resistant starch (RS) content, however, was greater for the uncooked flours than that for cooked kernels. The cooked kernels of 3 cereal samples tested showed higher glycemic index (GI) values than the uncooked flours.

Nutrient digestibility in black soldier fly larva was greater than in adults for pigs and could be estimated using fiber

  • Jongkeon Kim;Kwanho Park;Sang Yun Ji;Beob Gyun Kim
    • Journal of Animal Science and Technology
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    • 제65권5호
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    • pp.1002-1013
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    • 2023
  • The objectives of the present study were to determine the nutrient digestibility of fish meal, defatted black soldier fly larvae (BSFL), and adult flies and to develop equations for estimating in vitro nutrient digestibility of BSFL for pigs. In vitro digestion procedures were employed to mimic the digestion and absorption of nutrients in the pig intestine. Correlation coefficients between chemical composition and in vitro nutrient digestibility of BSFL were calculated. In Exp. 1, in vitro ileal digestibility (IVID) of dry matter (DM) and crude protein (CP) and in vitro total tract digestibility (IVTTD) of DM and organic matter in defatted BSFL meal were less (p < 0.05) than those in fish meal but were greater (p < 0.05) than those in adult flies. In Exp. 2, CP concentrations in BSFL were negatively correlated with ether extract (r = -0.91) concentration but positively correlated with acid detergent fiber (ADF; r = 0.98) and chitin (r = 0.95) concentrations. ADF and chitin concentrations in BSFL were negatively correlated with IVID of DM (r = -0.98 and -0.88) and IVTTD of DM (r = -1.00 and -0.94) and organic matter (r = -0.99 and -0.98). Prediction equations for in vitro nutrient digestibility of BSFL were developed: IVID of CP (%) = -0.95 × ADF (% DM) + 95 (r2 = 0.75 and p = 0.058) and IVTTD of DM (%) = -2.09 × ADF + 113 (r2 = 0.99 and p < 0.001). The present in vitro experiments suggest that defatted BSFL meal was less digestible than fish meal but was more digestible than adult flies, and nutrient digestibility of BSFL can be predicted using ADF as an independent variable.

Development of a Dynamic System Simulating Pig Gastric Digestion

  • Chiang, C.-C.;Croom, J.;Chuang, S.-T.;Chiou, P.W.S.;Yu, B.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권10호
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    • pp.1522-1528
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    • 2008
  • The objective of this study was to develop a model for simulating gastric digestion in the pig. The model was constructed to include the chemical and physical changes associated with gastric digestion such as enzyme release, digestion product removal and gastric emptying. Digesta was collected from the stomach cannula of pigs to establish system parameters and to document the ability of the model to simulate gastric digestion. The results showed that the average pH of gastric digesta increased significantly from 2.47 to 4.97 after feed consumption and then decreased 140 min postprandial. The model described the decrease in pH within the pigs' stomach as $pH_t=5.182e^{-0.0014t}$, where t represents the postprandial time in minutes. The cumulative distribution function of liquid digesta was $V_t=64.509e^{0.0109t}$. The average pepsin activity in the liquid digesta was 317Anson units/mL. There was significant gastric emptying 220 min after feed consumption. The cybernetic dynamic system of gastric digestion was set according to the above data in order to compare with in vivo changes. The time course of crude protein digestion predicted by the model was highly correlated with observed in vivo digestion (r = 0.97; p = 0.0001), Model prediction for protein digestion was higher than that observed for a traditional static in vitro method (r = 0.89; p = 0.0001).

단백질(蛋白質) 분해효소(分解酵素) 첨가시(添加時) 우육(牛肉)의 숙성(熟成)에 관(關)한 연구(硏究) -VI. Papain처리(處理)가 우육(牛肉)의 소화율(消化率)에 미치는 영향(影響)- (Studies on the Aging of Beef at Adding the Proteolytic Enzyme -VI. Effects of Papain Treatment on the Enzymatic Digestibility of Beef-)

  • 윤정의
    • 한국식품과학회지
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    • 제10권4호
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    • pp.394-397
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    • 1978
  • 한우(韓牛)의 round muscle에 papain으로 일차(一次) 분해(分解)시킨 후 pepsin에 의한 in vitro소화율(消化率)과 소화과정중(消化過程中) 생성(生成)되는 amino 태(態)-N의 경분적(經分的)인 변화(變化)를 실험(實驗)한 결과(結果)는 다음과 같다. 1. 효소처리구(酵素處理區)가 대조구(對照區)에 비(比)하여 소화율(消化率)도 높았으며 소화시간(消化時間)도 단축되었다. 2. 효소처리구(酵素處理區)는 소화과정중(消化過程中) $6{\sim}10$분간(分間)사이에 소화율(消化率)이 현저하게 증가(增加)하였다. 3. amino태(態)-N는 소화율(消化率)과 소화시간(消化時間)에 거의 비슷하게 증가(增加)하였다. 4. 대조구(對照區)에 비(比)하여 효소처리구(酵素處理區)가 amino태(態)-N 생성량(生成量)이 현저하게 높았으며$2{\sim}8$시간(時間)사이에서 그 증가율(增加率)이 가장 높았다.

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EFFECT OF PLANT PHENOLIC ACIDS ON CELLULOLYTIC ACTIVITY OF MIXED RUMEN POPULATIONS

  • Ushida, K.;Watase, H.;Kojima, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제3권1호
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    • pp.27-31
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    • 1990
  • Influences of plant phenolic acids and their possible metabolites(non-phenolic aromatic acids involved) in the rumen on the cellulolytic activity of mixed rumen populations were examined by a simple in vitro culture technique. Initial concentrations of aromatic acids were 1, 5, 10 and 20 mM/l. All the tested aromatic acids reduced microbial cellulose digestion especially at the higher initial concentration. P-Coumaric acid, ferulic acid and cinnamic acid, those having unhydrogenated propenoic side chain were more inhibitory than were 3-phenylpropinic acid and phloretic acid, those having hydrogenated propanoic side chain. Lag-time for cellulose digestion was prolonged by former three acids by 16 h. Apparent reduction in p-coumaric acid concentration was observed at 24 h when cellulose digestion began. Volatile fatty acid productions from cellulose fermentation were shifted by former three aromatic acids to produce more acetate and less propionate. This suggests that the selection of celluloytic organisms was induced by these aromatic acids.

An In vitro Enzymatic Digestion Method for Estimation of the Acrylamide Contents of Foods

  • Kim, So-Hyun;Yoon, Ko-Woon;Kim, Mi-Kyo;Paek, Se-Hee;Choi, Dong-Mi;Oh, Sang-Suk;Park, Jin-Byung
    • Food Science and Biotechnology
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    • 제16권3호
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    • pp.493-495
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    • 2007
  • In this study, the acrylamide contents of foods were estimated via liquid chromatography (LC)/mass spectrometry (MS)/MS after the food matrix constituents had been degraded with digestive enzymes (i.e., pepsin and pancreatin) and extracted with water. The quantities of acrylamide released from samples of cereal, potato chips, peanuts, and coffee were $62{\pm}5.1,\;970,\;106{\pm}20$, and 890 ppb, respectively. No acrylamide was detected in samples of soybean curd (tofu), fish cake, and ham. Compared to the amounts of acrylamide detected after extraction with water only, we noted no significant differences in the soybean curd, fish cake, potato chip, ham, and coffee samples. However, the quantities of acrylamide released from the cereal and peanut samples were approximately 2-fold larger following pretreatment with the digestive enzymes. This study presents a new in vitro enzymatic digestion method which allows for a more accurate estimation of the acrylamide contents of foods.

Effect of heat treatment of digestion-resistant fraction from soybean on retarding of bile acid transport in vitro

  • Han, Sung-Hee;Lee, Seog-Won;Rhee, Chul
    • Nutrition Research and Practice
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    • 제3권2호
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    • pp.149-155
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    • 2009
  • In this study, we investigated the heat effect of digestion-resistant fraction (RF) from soybean on retarding bile acid transport in vitro. The RFs from soybean retarded bile acid transport. A raw, unheated RF of soybean (RRF-SOY) was significantly more effective than the heated RF of soybean (HRF-SOY). The RS1 which physically trapped in milled grains and inaccessible to digestive enzyme after 18 hrs incubation level of content in RRF-SOY was found to be as high as 24.1% and after heating the RS1 of HRF-SOY was significantly reduced to 16.8%. The X-ray diffraction pattern of RF from soybean was altered after heat treatment. The RFs from soybean were characterized by peak at diffraction angles of $12.0^{\circ}$ and $20.0^{\circ}$ corresponding to RS content. Cellulose contents of RRF-SOY was 5% higher than that of HRF-SOY and pentosan contents of RRF-SOY was 5% higher than that of HRF-SOY, too. Whereas the hemicellulose content of RRF-SOY was 13% lower than HRF-SOY.

효소변형 전분기반 하이드로젤 모델 프리믹스 내 탑재된 커큐민의 소화과정 중 안정성 (Stability of the enzyme-modified starch-based hydrogel model premix with curcumin during in vitro digestion)

  • 강지현;노신정;이지영;김용노
    • 한국식품과학회지
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    • 제53권4호
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    • pp.365-374
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    • 2021
  • 본 연구에서는 커큐민 함유 에멀션의 안정성을 위해 필드하이드로젤로 쌀전분(RS), 1시간 효소처리한 전분(1GS), 24시간 효소처리한 전분(24GS), 96시간 효소처리한 전분(96GS)을 사용하였으며 이를 이용한 모델 프리믹스로 커큐민 탑재 필드하이드로젤 분말(FHP)을 제조하여 탑재된 커큐민의 안정성 및 in vitro 소화 후 커큐민 보유율의 변화를 관찰하였다. FHP는 커큐민 함유 에멀션분말(EMP)과 비교하여 재분산시 크리밍 현상없이 분산안전성을 보여주었다. 재분산 하이드로젤은 고온에서 녹으면서 오히려 액적(droplet)의 유착(coalescence)과 응집(flocculation)이 가속화되어 에멀션에 비해 열안정성이 떨어졌으나 UV 안정성은 RS-FHP와 1GS-FHP에서 유의적인 커큐민 보호 효과를 나타냈다. GS-FHP는 in vitro 소화 중 기름방울의 응집 및 유착이 발견되지 않았으며 소화 후 에멀션의 유상에 용해된 커큐민을 성공적으로 보호함을 확인하였다. 따라서, GS-FHP는 분말형태 프리믹스 개발에 새로운 소재로 사용 가능할 것이며 커큐민 외에 다른 다양한 소수성 기능성물질에 대해서도 적용을 확장할 수 있을것으로 생각된다.