• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2004년도 추계학술발표회
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• pp.43-47
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• 2004

두 개의 다른 위성 센서 (RADARSATSAR와 KOMPSAT EOC)를 사용하여 선박탐지 실험을 실시하였으며, 탐지성능을 검토하였다. 목포항과 울산항을 대상으로 실시하였으며 필요에 따라 현장 검증 데이터를 얻기 위하여 위성 통과 시에 선박에 승선하여 선박정보를 포함하는 Sea Truth를 취득하였다. 또한 VTS레이더 정보를 위성데이터와 비교를 통하여 위치정보의 검증을 수행하였다. 광학과 마이크로파 원격탐사에 있어 그 특성의 차이는 뚜렷하였으나, 광학의 경우 선속 3.1kts 이상인 선박의 후류가 탐지되었으며, 마이크로파의 경우, 최대 6-7kts의 선박에 대해서도 후류의 탐지는 어려웠다. 그러나, 마이크로파는 다양한 선형을 반영한 신호가 sigma nought로 표현되므로 향후 선형 정보뿐만 아니라, 침로의 추출이 가능하다는 결론을 얻었다. 또한, 동일 선박이라 할지라도 신호강도의 차이에 의해 선박이 2개 이상으로 나타나는 현상도 파악되었다. 앞으로 다양한 해상환경 및 위성 관측 모드에 따른 추가 실험을 실시하여 자동 선박추출이 가능할 것으로 판단된다.

• Smart Structures and Systems
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• 제5권4호
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• pp.317-328
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• 2009

Current maintenance operations and integrity checks on a wide array of structures require personnel entry into normally-inaccessible or hazardous areas to perform necessary nondestructive inspections. To gain access for these inspections, structure must be disassembled and removed or personnel must be transported to remote locations. The use of in-situ sensors, coupled with remote interrogation, can be employed to overcome a myriad of inspection impediments stemming from accessibility limitations, complex geometries, the location and depth of hidden damage, and the isolated location of the structure. Furthermore, prevention of unexpected flaw growth and structural failure could be improved if on-board health monitoring systems were used to more regularly assess structural integrity. A research program has been completed to develop and validate Comparative Vacuum Monitoring (CVM) Sensors for surface crack detection. Statistical methods using one-sided tolerance intervals were employed to derive Probability of Detection (POD) levels for a wide array of application scenarios. Multi-year field tests were also conducted to study the deployment and long-term operation of CVM sensors on aircraft. This paper presents the quantitative crack detection capabilities of the CVM sensor, its performance in actual flight environments, and the prospects for structural health monitoring applications on aircraft and other civil structures.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 총회 및 춘계학술발표회
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• pp.22-25
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• 2002

The effect of in-situ chemical oxidation on the indigenous soil microorganisms (total microbes and PAH-degrading microbes) and contaminant removal were investigated. Field soil contaminated with diesel in gas station was collected and the soil was treated from 0 to 900 minutes by in-situ ozonation as chemical remediation. The treated soil samples were incubated with supplying oxygen during the 9 weeks to understand the characteristics of microbes regrowth, damaged by ozone. The sharp decrease of aromatic fraction and TPH was observed within 60 minutes of ozone application and aromatic fraction and TPH then slowly decreased. The phenanthren-degrading bacteria were the most sensitive to ozonation, because 1 hour of ozonation reduced the microbes from 10$^{6}$ CFU/g-soil to below detection limits.

• 미생물학회지
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• 제37권3호
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• pp.204-208
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• 2001

Bacillus속에 속하는 세균을 FISH 방법으로 검출하기 위한 새로운 방법을 개발하였다. LGC353b, S-G-Bacill-0597-a-A-22와 S-G-Bacill-0597-a-S-22 등 3개의 probe를 22종의 Bacillus속 세균의 혼합배양액에 적용한 결과 S-G-Bacill-0597-a-A-22의 probe가 DAPI로 검출된 세균 중 95%가 FISH로도 측정되어 가장 효율이 좋은 것으로 나타났다. Hybrillization 과정 전에 Bacillus의 영양세포와 포자의 세포벽을 파괴하기 위하여 SDS/DTT (dithiothreitol)와 lysozyme를 순차적으로 전처리하는 방법과 lysozyme용액으로만 전처리 하는 두 가지 방법을 사용하였다. 그 결과, Bacillus를 검출하기 위해서는 영양세포의 경우에는 lysozyme용액으로만 전처리 하는 방법이 가장 좋았으며, 포자는 FISH로 검출되지 않았다. 따라서 토양, 하수 처리장등의 생태계에서 Bacillus를 검출할 경우에는 영양세포는 lysozyme으로 전처리 하여 FISH로 검출하고 포자는 따로 포자염색으로 검출하는 두가지 방법을 병행하여야한다.

• Food Science and Biotechnology
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• 제16권1호
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• pp.99-103
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• 2007

The purpose of this study was to develop a simple detection method, possibly at the species-level, that allows for large-scale screening of bifidobacteria. Human fecal samples were plated on MRS-raffinose agar containing cysteine and neomycin sulfate, serving as selective pressure for bifidobacteria, and 0.003%(w/v) bromocresol green. All of the test strains grew well on this medium at $37{\pm}1^{\circ}C$, forming white colonies surrounded by yellow halos, which presented a sharp contrast against the green background. In this disc assay, the required incubation time to develop a yellowish zone varied with the species of Bifidobacterium that was tested, allowing for differential counts and easy identification at the species-level: 10-14 hr for B. bifidum, 20-22 hr for B. catenulatum and B. infantis. and 24-25 hr for B. longum and B. breve. No apparent color was observed for B. angulatum and B. adolescentis 28 hr after inoculation. To evaluate the results of pH indicator-based identification, individual isolates were subjected to a colony-PCR experiment with genus-specific primers. The amplified products from the isolates were in good accordance with those from the reference strains at a level of 95% agreement. These results suggest that the present method could be conveniently applied to cell counts, as well as to the preliminary identification of bifidobacteria from a variety of sample types including human feces, dairy products, and commercial probiotic supplements.

• Parasites, Hosts and Diseases
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• 제34권3호
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• pp.177-184
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• 1996

흰쥐의 폐조직 절편에서 실험적으로 유발시킨 폐포자충을 검출하고자 조직내교잡법 (in situ hybridization)을 수행하였다 흰쥐는 methylprednisolone(데포-메드롤)을 10 mg/kg의 양으로 주 1회 피하주사하여 면역억제시켰고. 6 8 및 9주에 희생시켜 폐를 적출한 후 포르말린에 고정하였다. 폐포자충의 5S rRNA에 상보적인 22 base길이의 oligonucleotide probe를 주문제작(한국 생공)하였고 3' 부위에 biotin을 부착하였다 조직내교잡법은 Microp「obi stainin료 system과 manual capillary action법을 이용하여 1시간 이내에 수행하였다 폐포자충은 주로 폐포세포의 내강면. 폐포내 삼출물 및 세기관지의 분비물 등에서 검출되었다 6주 면역억제시 양성반응은 주 로 폐단면의 가장자리 부위에서 부분적으로 간찰되었으나. 8 내지 9주에서는 전체적으로 관찰되었 나. Grocott의 methenamine silver 염색법에 비하여 조직내 교잡법은 폐포자충을 신속하게 검출 할 수 있호 영양형의 검출에는 특히 유리하며. 다른 알려진 병원성 진균 및 원충류와의 교차반응이 없어. 실험적으로 유발시킨 폐포자충에 의한 폐렴의 진단에도 유용하게 사용될 수 있을 것으로 생각된다.

• Transactions on Electrical and Electronic Materials
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• 제6권6호
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• pp.262-271
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• 2005

In order to achieve timely and accurate fault detection of plasma etching process, neural network based time series modeling has been applied to reactive ion etching (RIE) using two different in-situ plasma-monitoring sensors called optical emission spectroscopy (OES) and residual gas analyzer (RGA). Four different subsystems of RIE (such as RF power, chamber pressure, and two gas flows) were considered as potential sources of fault, and multiple degrees of faults were tested. OES and RGA data were simultaneously collected while the etching of benzocyclobutene (BCB) in a $SF_6/O_2$ plasma was taking place. To simulate established TSNNs as incipient fault detectors, each TSNN was trained to learn the parameters at t, t+T, ... , and t+4T. This prediction scheme could effectively compensate run-time-delay (RTD) caused by data preprocessing and computation. Satisfying results are presented in this paper, and it turned out that OES is more sensitive to RF power and RGA is to chamber pressure and gas flows. Therefore, the combination of these two sensors is recommended for better fault detection, and they show a potential to the applications of not only incipient fault detection but also incipient real-time diagnosis.

• 대한수의학회지
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• 제36권4호
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• pp.859-871
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• 1996

This study was conducted to elucidate the distribution of Aujeszky's disease viral nucleic acids and antigens in the central nervous system (CNS) of piglets. The first Korean isolate of Aujeszky's disease virus(ADV) that isolated from naturally infected piglets in Yang San, was inoculated into 32 day old piglets with $10^{5.9}TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at every 24hrs for 8 days. The immunohistochemistry (IHC) was conducted to detect the viral antigens in paraffin-embedded tissue sections using avidin-biotin-peroxidase complex (ABC) method. The viral nucleic acids were detected by in situ hybridization (ISH) using ADV specific DNA probe labeled with digoxigenin. The ADV antigens were detected in reticuloendothelial cells of spleen, lymph nodes and tonsil, alveolar walls, leptomeningeal vascular walls, inflammatory foci of each organ, and nerve cells. The viral nucleic acids were detected in the spinal trigeminal nucleus and its tracts of the pons and medulla oblongata by the ISH technique. The pathways of AD viruses in CNS were determined by IHC and ISH. In the intranasally inoculated group, the viruses in nasal mucosa moved to medulla oblongata and pons through the trigeminal nerve. In case of intramuscullarly inoculated group, viruses moved to brain via lymphoid organs or spinal nerves from sciatic nerves.

• 대한수의학회지
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• 제39권3호
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• pp.531-537
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• 1999

The present study was carried out to investigate the pathogenicity and pathogenesis of wild rats(Rattus norvegicus), trapped in nature, intranasally infected with Aujeszky's disease virus(ADV/NYJ-1-87) by histopathology, immunohistochemistry and in situ hybridization(ISH). Fifteen rats inoculated intranasally were roughened haircoat, anorexia, listlessness, and depression second day after inoculation, and three rats died in 66-72 hours. Eight rats showed severe pruritus at the face that was accompanied by frequent face-washing movements of the forelegs, and then became violent and spasmodic for an hour or until they died. Four rats slowly recovered after showing mild clinical signs of the disease. Microscopic lesions in infected rats were characterized by meningitis, perivascular round cell infiltration, focal gliosis, and neuronal degeneration and necrosis. And intranuclear inclusion bodies were frequently detected in the cerebral cortex and medulla. Positive reaction to ADV by immunohistochemistry and ISH were detected in the following areas : trigermimal ganglion, brain, tonsil, nasal mucosa, spleen, lung and liver. The result has suggested that ADV intranasally infected in wild rats is followed by replication in epithelial calls of nasal mucosa and tonsil, then invade local lymph nodes by way of the lymphatics. It is also believed that the virus invades bipolar olfactory cells and trigerminal ganglion; and then spread into central nervous system.

• Current Optics and Photonics
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• 제7권1호
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• pp.38-46
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• 2023

Optical Fourier-domain reflectometry (OFDR) sensors have been widely used to measure distances with high resolution and speed in a noncontact state. In the electroplating process of a printed circuit board, it is critically important to monitor the copper-plating thickness, as small deviations can lead to defects, such as an open or short circuit. In this paper we employ a phase-based OFDR sensor for in situ relative distance sensing of a sample with nanometer-scale resolution, during electroplating. We also develop an optical-path difference (OPD)-regulated sensing probe that can maintain a preset distance from the sample. This function can markedly facilitate practical measurements in two aspects: Optimal distance setting for high signal-to-noise ratio OFDR sensing, and protection of a fragile probe tip via vertical evasion movement. In a sample with a centimeter-scale structure, a conventional OFDR sensor will probably either bump into the sample or practically out of the detection range of the sensing probe. To address this limitation, a novel OPD-regulated OFDR system is designed by combining the OFDR sensing probe and linear piezo motors with feedback-loop control. By using multiple OFDR sensors, it is possible to effectively monitor copper-plating thickness in situ and uniformize it at various positions.