• 분석과학
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• 제28권2호
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• pp.79-85
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• 2015

TLC (SiO₂) 상에서 3가지 유사 암페타민 류 아민 화합물과 3가지 유도체화 시약 사이의 반응으로부터 얻어진 아민유도체의 이미지 분석으로부터 암페타민 류 화합물에 대한 신속하고 경제적인 반정량적인 분석방법을 제시하기 위하여 선행 실험을 하였다. TLC상 직접적인(in situ, co-spot) 유도체화 반응의 적정화를 시도하였고 그 TLC 결과물들을 디지털카메라로 촬영하여 이미지를 얻은 후 2가지의 이미지 분석 프로그램(CP Atlas 2.0 및 ImageJ)에 적용하여 재현성(RSD; %)과 상관성(R²)을 각각 확인 및 비교하였다. 그 결과, 반응 조건에 대한 조정이 필요하였고(반응 온도 등) 각 시료별 2가지의 농도(0.5 mg/mL 및 0.01 mg/mL)에서 반응의 재현성은 0.69~5.50% 범위이었으며 반응(2 µL per spot)의 생성물과 아민의 농도에 대한 상관성은 0.1~0.005 mg/mL의 농도 구간에서 가장 높게 나타났다(R²> 0.9906). 또한, 두 가지 프로그램 모두 재현성과 직선성에 대한 유사한 결과를 나타내어 직접적인 유도체화 반응과 TLC 이미지에 대한 이들 프로그램을 사용한 분석이 아민 화합물에 대한 반정량적인 분석으로 사용될 수 있는 가능성을 보였다.

• 한국연초학회지
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• 제12권1호
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• pp.29-38
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• 1990

Until relatively recently plant scientists have made little use of immunological techniques. Now, however, more and more researchers are discovering the powder of these techniques for the screening of immunomodulators and for the detection, quantitative determination and localization of compounds in plant materials. Especially, the recent developments in the fields of plant biotechnology and plant genetic engineering make it even more important for forkers in the plant sciences to become acquainted with the more sophisticated methods. The possible methods include onestep purification of antigens, visualization in situ by immunocytochemis try and on polyacrylam ids gel s by ni trocellulose Western blotting and quantification by various immunoassay. Among them, in this reviews, the quantitative determination methods are to be reviewed. There are several kinds of methods for the quantitative determination of plant constituents such as colorimetry, TLC, GLC, DCC, UV derivatization, densitometry and HPLC. When the complexity of plant constituents is considered. densitometry and HPLC have many advantages in sensitivity and separation ability. After a 11 some advarltages of two methods meritiorled above, all of these methods have many disadvantages and inconveniences. Previous purification for the application of all these methods make them less sensitive and more tedious. Immunoassay can solve these problems in part. But immunoassay also has some limitations. Specificity of immunoassay, contrary, can be considered to be disadvantages. Including this the advantages and disadvantages of immunoassay are to be discussed.

• Archives of Pharmacal Research
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• 제23권3호
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• pp.226-229
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• 2000

To obtain the standard compounds of metoprolol for a pharmacokinetic study, a convenient synthetic procedure to prepare enantiomers of metoprolol (3a) and its major metaboites, 2-4-(2-hydroxy-3-isopropylamino)propoxyphenylathanol (3b) and 4-(2-hydroxy-3- isopropylamino) pro-poxyphenylacetic acid (4), was developed from their respective starting materials, 4-(2-methoxyethyl)phenol (1a), 4-(2-hydroxyethyl)phenol (1b) and methyl 4-hydroxyphenylacetate (1c). These phenolic compounds (1a, b, c) were converted in situ to their corresponding phenoxides with sodium hydroxide treatment followed by (R)- or (S)-epichlorohydrin treatment. The resulting epoxides 2 were transformed to 3 through reaction with isopropylamine. Ester 3c was hydrolyzed to the metabolite 4. Measured using the HPLC method on chiral column without any derivatization, the optical purity of enantiomers of metoprolol and o-demethylated metabolite 3b ranged between 96-99 ％ ee and that of enantiomers of carboxylic acid metabolite 4 ranged 91％ ee.