• 한국지하수토양환경학회지:지하수토양환경
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• 제6권2호
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• pp.39-47
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• 2001

회분식 세척실험에서 적용된 혼합계면활성제 중 $POE_5$ + $POE_14$, SDS+$POE_5$가 endosulfan의 탈착에 효율이 우수한 것으로 나타났으며, 독성실험 결과 SDS+POE$_{5}$는 미생물의 활성을 다소 저해시키는 경향이 있어 $POE_5$ + $POE_14$ (1:1) 1%를 세척제로 선정하였다. In-Situ flushing 실험에서는 세척용액의 주입 flux가 1.5L/min/m$^2$일 때 67%의 제거효율을 얻을 수 있었으며, 보조용매로서 메탄올과 에탄올을 첨가한 경우에는 각각 75%와 81%의 제거효율을 보였다. In-situ flushing기법의 적용에 따른 농도 저감이 생물학적 분해에 미치는 영향을 알아보기 위하여, 초기 농도 13mg/kg dry soil과 3mg/kg dry soil인 토양을 생물학적으로 처리한 결과 제거 효율은 각각 86% 및 81%였으며, 2가지 토양 모두 24시간 이후에는 미생물에 의한 작용이 크지 않은 것으로 나타났다. 이것은 토양상에서 용액상으로의 물질전이 속도가 율속 단계로 작용하고 있기 때문이라 판단된다. 토양에 잔류하는 계면활성제가 생물학적 분해에 미치는 영향에 대하여 검토한 결과, 잔류하는 계면활성제에 의해 물질전이 속도가 향상되어 생분해가 지속적으로 일어났으며 초기 농도 3mg/kg dry soil인 경우 120시간이 경과한 후 89%의 제거효율을 나타내었다. 계면활성제와 보조용매가 동시에 잔류하는 경우에는 계면활성제에 대한 순응기간이 보다 길어지는 것을 알 수 있었으며, 메탄올과 에탄올의 경우 각각 84% 및 83%의 제거효율을 나타내었다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 International Symposium
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• pp.89-110
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• 2003

최근 현장(In situ)에서의 토양 및 지하수 오염 정화는 오염물질을 현장에서 추출해 지상에서 처리하는 것이 아니라, 직접 현장에서 미생물 혹은 화학물질과 오염물질의 혼합을 통한 분해기 작을 이용해, 오염물질을 제거하는 과정을 의미한다. 이러한 현장오염정화 기법 중 하나가 Figure 1에 도시한 수직흐름처리정(Horizontal Flow Treatment Wells, HFTWs)으로 2개의 정(well) 중간지점에 반응물질을 설치한 후 각각의 정에서 상향 및 하향흐름을 발생시켜 주위의 지하수를 순환시키면서 정화하는 기법이다.

• 한국미생물·생명공학회지
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• 제39권2호
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• pp.161-167
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• 2011

본 연구실에서 확보한 diesel 분해 고효율 균주 Pseudomonas putida KDi 19, kerosene 분해 고효율 균주 P. aeruginosa K14, gasoline 분해 고효율 균주 P. putida G8, BTEX 분해 고효율 균주 P. putida BJ10, P. putida E41의 5개의 고효율 균주를 컬럼 및 반응기에 적용하여 TPH의 생물학적 분해 실험에 적용하였다. 영양염류 및 산소 농도, 균농도 등 최적의 환경인자 도출을 통해 최적의 생물학적 처리 효율을 TPH의 경우, MSM 및 activator I을 주입하여 25일 동안 76.3%의 제거 효율과 제거속도상수 K=0.711를 나타냈으며, diesel의 경우 40일 동안 99.2%의 제거 효율을 보였다. 또한, TPH 오염 토양의 lab-scale bioremediation 실험에서 고효율 균주를 적용한 결과 45일 운전 기간 동안 7,209.9 $mg{\cdot}kg^{-1}$을 825.6 $mg{\cdot}kg^{-1}$까지 88.5% 제거하였다. 본 연구에서 도출된 TPH로 오염된 토양의 bioremediation을 위한 고효율 균주 확보와 최적의 환경 인자 도출은 현재 부족한 생물학적 처리 연구와 물리적 화학적 처리의 문제를 해소하기 위한 기초적 실험 자료로서 기여할 것으로 사료된다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 1998년도 공동 심포지엄 및 추계학술발표회
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• pp.232-235
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• 1998

Air sparging system is a kind of in-situ bioremediation method in the contaminated ground water. When Air sparging, the both of water circulation and oxygen transfer happend in the same time. The hydraulic differential head is zero at the middle height of well, is negative below the height and is possitive above the height. Hydroraulic head gradient is proportioned to air superficial velocity in the well. But over 24m/min of the superficial velocity, the hydraulic head gradient increase little.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1992년도 폐기물 매립지의 공학적 특성과 개량기술
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• pp.1-18
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• 1992

The contaminatlon of soil and groundwater by leachate from impmperly managed landfills, or by cheiicals and gasoline leaked flu underground storage tanks has buou a serious urldwide environmental problei. Most of those contaminants are adsorptive and absorptive into soul, while they are hardly soluble in water. Thus, the rate of self purification is very slow, causing persistent problems in water use and environmental protection when the contamination is left untreated. Biological remediatlon technologies utilize the ertraordlnary caperbllity of microorganisms In degrading a tilde spectrum of organic compounds. Among them, an in situ bioremediation technology Involves injection of supplementary materials into the subsurfce in order to bring about a significant Increase in the microbial activity. The Increased microbial activity helps remove the pollutants in situ, that is, without digging out contaminants, soil, or water. This paper focused on the features, possibilities, and limitations of the bioremedition technology.

• 상하수도학회지
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• 제18권3호
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• pp.307-311
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• 2004

The objective of this work is to evaluate the hypothesis that a good technique for supplying oxygen to the saturated zone in the presence of light nonaqueous phase liquid (LNAPL) pool contamination at the water table is to pass air through the unsaturated zone above the pool. This hypothesis was evaluated in experimental studies performed using a bench-scale, sand-tank reactor, Steady-state abiotic experiments in the sand-tank reactor with air flowing through the reactor headspace demonstrated that oxygen supply through the water table interface into the saturated zone was enhanced when an LNAPL (dodecane) pool was present at the water table. These experimental results confirmed the hypothesis that an LNAPL pool can serve as a high concentration oxygen source to the oxygen-limited area beneath the pool and, as a result, enhance the in situ biodegradation rate.

• 상하수도학회지
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• 제18권3호
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• pp.337-342
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• 2004

Previous experiment was performed under abiotic condition. Steady-state abiotic experiments in the sand-tank reactor with air flowing through the reactor headspace demonstrated that oxygen supply through the water table interface into the saturated zone was enhanced when an LNAPL (dodecane) pool was present at the water table. Biotic condition was considered in this study. Biotic experiments performed after inoculating the reactor with Pseudomonas putida mt-2, which does not grow on dodecane, indicated that the enhanced oxygen supply in the presence of the LNAPL pool also enhanced biodegradation of a solute (glucose) plume passing beneath the LNAPL pool at steady-state.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.175-178
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• 2004

Little study has been published specifically addressing the dynamics of nitrate reducing bacteria (NBR) during the bioremediation of nitrate-contaminated aquifer. In our previous study we successfully quantified fumarate-enhanced microbial nitrate reduction rate in a nitrate-contaminated aquifer by using a series of single-well push-pull tests (PPTs). In this study we analyzed the suspended population during PPTs. To monitor changes in the microbial community, PCR amplification of 16S rDNA genes and denaturing gradient gel electrophoresis (DGGE) were used to study the dynamics of the bacterial community in detail. Before the stimulation of NBR, the dominant DGGE bands obtained by PCR were affiliated with V-Proteobacteria consisting of Acinetobacter spp. and Pseudomonas fluorescens. However, as NBR biostimulation proceeded, the dominant patterns of DGGE bands changed, and they were affiliated with Azoarcus denitrificans Td-3 and Flavobacterium xanthum. Azoarcus denitrificans Td-3 is known to completely reduce nitrate to nitrogen gas. The series of single-well push-pull tests in this study should prove useful for conducting rapid, low-cost feasibility assessments for in situ denitrification and provide important information about which microorganisms play a key role in bioremediation of a nitrate contaminated aquifer.

• 한국환경과학회지
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• 제17권1호
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• pp.97-105
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• 2008

This study examined the contaminated soils with an indicator of TPH using SVE (Soil Vapor Extraction) and biological treatments. Their results are as follows. Water content in the polluted soils slowly decreased from 15% during the initial experimental condition to 10% during the final condition. Purification of polluted soils by Bioventing system is likely to hinder the microbial activity due to decrease of water content. Removal rate of TPH in the upper reaction chamber was a half of initial removal rate at the 25th day of the experiment. The removal rate in the lower reaction chamber was 45% with concentration of 995.4 mg/kg. When the Bioventing is used the removal rate at the 14th day of the experiment was 53%, showing 7 day shortenting. Since the Bioventing method control the microbial activity due to dewatering of the polluted soil, SVE method is likely to be preferable to remove in-situ TPH. The reactor that included microbes and nutrients showed somewhat higher removal rate of TPH than the reactor that included nurtients only during experimental period. In general, the concentration showed two times peaks and then decreased, followed by slight variation of the concentration in low concentration levels. Hence, in contrast to SVE treatment, the biological treatment tend to show continuous repetitive peaks of concentration followed by concentration decrease.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.678-682
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• 2005

The promoter region of a carbon starvation gene isolated from Pseudomonas putida was cloned and analyzed for its potential use for in situ bioremediation and bioprocessing. We constructed a recombinant plasmid pMKD101 by cloning the 0.65 kb promoter region of the gene into the promoter proving vector, pMK301, which contains the lacZ for ${\beta}$-galactosidase activity as a reporter gene. pMKD101 was transformed into the wild-type P. putida MK1, resulting in P. putida RPD101, and analyzed for ${\beta}$-galactosidase activity under different culture conditions. When RPD101 was grown on the minimal medium plus $0.1\%$ glucose as a sole carbon source in batch cultures, ${\beta}$-galactosidase activity was found to be 3.2-fold higher during the stationary phase than during the exponential phase. In chemostat cultures, ${\beta}$-galactosidase activity was found to be 3.1-fold higher at the minimal growth rate (dilution rate=$0.05\;h^{-1}$) than at the maximal growth rate (dilution rate=$0.173;h^{-1}$). The results suggest that a carbon starvation promoter can be utilized to maximize the expression of a desired gene under nutrient limitation.