• Proceedings of the Korean Geotechical Society Conference
• /
• 2001.03a
• /
• pp.405-410
• /
• 2001

Bioremediation is a degradation process of existing organic contaminants in soils and groundwater by indigenous or inoculated microorganisms. This process can provide economical solution as well as safe and effective alternative in remediation technologies. However, it has been suggested that the rate of bioremediation process of organic contaminants by microorganisms can be limited by the concentration of nutrients and TEAs(Terminal Electron Accepters). In in-situ bioremediation, conventional pumping techniques have been used for supplying these additives. However, the injection of these additives is difficult in low permeable soils, and also hindered by preferential flow paths resulting from heterogeneities in high permeable ground. Therefore, the Injection of chemical additives is the most significant concern in in-situ bioremediation. Most recently, electrokinetic technique has been applied into the bioremediation and the injection characteristics under electrokinetics have not been examined in various soil types. Therefore, in this study, electrokinetic injection method is investigated in kaolinite and sand, and the concentration of ammonium(nutrients) and sulfate(TEAs) in soil is presented.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.04a
• /
• pp.192-195
• /
• 2003

As a part of our research project for in-situ bioremediation of nitrate contaminated. groundwater, screening studies to determine an effective electron donor (EO) and/or carbon source (CS) such as acetate, ethanol, formate, fumarate, lactate, and propionate were conducted. To evaluate the feasibility for the biological degradation of nitrate, soil microcosm studies using nitrate-contaminated soil and groundwater were performed. The nitrate removal percentage in the order from the highest to the lowest was: formate, fumarate, and ethanol > lactate > propionate. Essentially no nitrate consumption was observed In acetate-fed microcosms. The order of nitrate removal rate from the highest to lowest was fumarate, formate, lactate, ethanol, and propionate. These results suggest that fumarate and formate are promising EDs/CSs for in-situ bioremediation of nitrate - contaminated oxygenated groundwater.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2002.04a
• /
• pp.199-202
• /
• 2002

Many methods have been developed for the remediation of contaminated soil and groundwater. Among those technologies, in-situ bioremediation is most likely to be cost-effective method for petroleum hydrocarbon contamination. But the in-situ bioremediation can require more time to remediate hydrocarbon-contaminated soil and groundwater than other methods. Therefore we intended to save time of in-situ bioremediation using a biological additive to activate indigenous microbes in soil. The additive, 'Inipol EAP 22' stimulates the growth of specific flora, significantly accelerating the speed at which hydrocarbons are biodegraded. And it hans been tested in accordance with protocol approved by the USEPA and is registered on the National Contingency Plan Product Schedule List. In the experiment, three soil samples contaminated with fuel oil were prepared in the same concentration. Inipol EAP 22 was not added to one sample and was added to the other two samples with 5% and 10% of hydrocarbon by weight respectively. And $CO_2$gas derived from bacterial respiration was analyzed in each samples for 15 days. As a result, 145% and 153% of $CO_2$ evolution (microbial respiration) against the sample without 'Inipol EAP 22' occurred in samples with 'Inipol EAP 22' addition of 5% and 10%, respectively

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.04a
• /
• pp.360-363
• /
• 2003

Bioremediation is often used for in situ remediation of petroleum-contaminated site. We studied the microbial degradation of hydrocarbon in an artificially diesel contaminated soil in laboratory microcosm. In control soil, about 30% of the initial TPH was diminished and the degradation of diesel oil was significantly enhanced by the addition of bioremediation agent (70% of TPH reduction).

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.04a
• /
• pp.103-106
• /
• 2003

This study was conducted to evaluate in-situ bioremediation ability of Bioil-D, microbial material for oil degradation, at a gas station that had been treated by SVE system. TPH concentrations and total contaminated soil volume were rapidly decreased after Bioil-D treatment. The performance of Bioil-D was also estimated based on the observation of microbial population at the soil samples and $CO_2$ concentration produced at the extraction wells. The field study showed a successful work of Bioil-D.

• 한국석유지질학회:학술대회논문집
• /
• autumn
• /
• pp.68-84
• /
• 1999

Biodegradation is a natural weathering process by microorganisms to decompose spilled oil or environmental contaminants. To accelerate this process, applying nutrients (fertilizer) or more microorganisms to naturally occurring microorganisms is called 'Bioremediation.' Presently, most popular response technique to spilled oil is mechanical cleanup using booms or skimmers. For the alternative to this technique, chemical dispersants, in-situ burning are used. Another promising alternative is bioremediation and it can clean oil contaminated seashore during enough time. In this paper, types of bioremediation technologies, its usage potential, and important consideration issues when applying this technique were summarized.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.8 no.4
• /
• pp.179-185
• /
• 2005

Polycyclic aromatic hydrocarbons (PAHs) are a kind of toxic environmental pollutants and has been accumulated usually in marine sediments. Due to their potential hazardous to human, removal of PAHs from environments has been great concern. In the present study, the effect of microbial inoculation and the supplementation of mixed form cyclodextrin (M-CD) was assessed in the pre-sterilized or nonsterilized microcosms for optimizing operational conditions for ex situ bioremediation of sediments contaminated by PAHs. Activity of electron transport system (ETSA) was increased by the addition of M-CD regardless of inoculation of microorganisms in microcosms without sterilization. The degradation rate of PAHs in sterilized microcosms was app. 9-20% by the inoculation of single strain and 24-37% by the inoculation of microbial consortium supplemented with 1% M-CD, respectively. The degradation was not observed in microcosms without sterilization under the same conditions. The proportion of inoculated microorganisms also decreased in nonsterilized microcosms. Signals of inoculated bacteria were decreased to detection limit after 2 days in the microcosms without M-CD. In conclusion, microbial inoculation with appropriate carbon sources and removal of natural flora and grazers are required for the efficient ex situ bioremediation of sediments contaminated by PAHs in bioslurry reactor.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.11
• /
• pp.1617-1626
• /
• 2013

The Bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. The dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, Rhizobium sp., Sinorhizobium, Ochrobactrum sp., Alcaligenes, Bacillus sp., Bacterium, Klebsiella sp., and Ensifer adhaerens. The bacterial community structure was altered extensively and its complexity reduced during the enrichment process, and four culturable bacteria, Ochrobactrum sp., Rhizobium sp., Geobacillus stearothermophilus, and Alcaligenes faecalis, remained in the final enrichment. Only one indigenous strain, BCL-1, with imidacloprid-degrading potential, was isolated from the sixth enrichment culture. This isolate was a gram-negative rod-shaped bacterium and identified as the genus Ochrobactrum based on its morphological, physiological, and biochemical properties and its 16S rRNA gene sequence. The degradation test showed that approximately 67.67% of the imidacloprid (50 mg/l) was degraded within 48 h by strain BCL-1. The optimum conditions for degradation were a pH of 8 and $30^{\circ}C$. The simulation of imidacloprid bioremediation by strain BCL-1 in soil demonstrated that the best performance in situ (tea soil) resulted in the degradation of 92.44% of the imidacloprid (100 mg/g) within 20 days, which was better than those observed in the ex situ simulations that were 64.66% (cabbage soil), 41.15% (potato soil), and 54.15% (tomato soil).

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2001.04a
• /
• pp.261-266
• /
• 2001

Three innovative technologies to remediate trichloroethylene (TCE) in situ were (or currently are being) evaluated at a TCE-contaminated groundwater site at Edwards Air Force Base (AFB), California. The three technologies all make use of groundwater recirculation to obviate the need to pump contaminated groundwater to the surface fer treatment. The first technology, which implements aerobic cometabolic bioremediation to destroy TCE in situ, successfully reduced dissolved TCE concentrations from above 1 mg/L to 20-30 $\mu\textrm{g}$/L. The second technology, in-well vapor stripping (IWVS), is capable of treating dissolved TCE at concentrations in the tens to hundreds of mg/L. Finally, the third technology, bioenhanced in-well vapor stripping (BEHIVS): is a combination of the first two technologies, and is designed to reduce very high levels of TCE (tens to hundreds of mg/L) to concentrations that meet regulatory requirements 5 $\mu\textrm{g}$/L). Results of field evaluations of tile first two technologies are presented, and the design of the BEHIVS system. as well as model predictions of BEHIVS performance and the current status of the technology field evaluation. is discussed.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2004.09a
• /
• pp.269-272
• /
• 2004

The use of colloidal gas aphron (CGA), as an external oxygen carrier, provides a promising alternative to promote aerobic bioremediation of BTEX in the subsurface environment. CGA is a stable bubble supported by three surfactant layers and can supply oxygen below the soil surface uniformly due to its plug-flow characteristic. Since CGA has a hydrophobic layer that can act as a partitioning medium for hydrophobic contaminants it is known to facilitate desorption of soil-sorbed contaminants. In addition, bioaugmentation and biostimulation are possibly achieved by using CGA when generated from a solution containing BTEX-degrading microorganisms and appropriate nutrients. In this study, we presented the physico-chemical characteristics of CGA generated from a solution composed of microorganisms and nutrients. The applicability of CGA as an in situ aerobic bioremediation technology of BTEX will be further evaluated.