• 한국양식학회지
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• 제9권1호
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• pp.83-92
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• 1996

농어목 어류, C. diagramma 암컷의 vitellogenin과 난황단백의 면역화학적 특성을 서로 비교하였다. 면역화학적 방법에 의해 C. diagramma 암컷의 혈청에서 vitellogenin 1 (VTG1)과 vitellogenin 2 (VTG2)를 동정하였으며, sephacryl S-300 gel 여과법에 의하여 측정된 분자량은 각각 560,000과 410,000이었다. 암컷의 난소 추출액에서는 두 가지 형태의 난황단백인 E2와 E3가 분리되었으며, 이들의 분자량은 각각 410,000과 170,000이었다. $17\beta$-estradiol ($E_2$)을 주사한 수컷의 혈청과 간조직을 abidine-biotin complex (ABC) 방법으로 분석한 결과, vitellogenin과 유사한 물질이 검출되었다. 이상의 결과로 부터, 외인성 $E_2$ 에 의해 간에서 합성된 vitellogenin은 혈액 속으로 방출된 후 난소로 이동되는 것으로 추정되었다. 암컷의 $E_2$, 혈중농도는 산란기인 6월에 최고치를 나타내었고, vitellogenin 상대농도도 $5\~6$월에 최고치를 나타내었다. $E_2$와 vitellogenin의 변화는 본 종의 난소성숙과 밀접한 관계가 있으며, 난황형성에 있어서도 중요한 역할을 하고 있음을 알 수 있었다. 암컷 혈청 VTG2와 난소추출액 E2의 분자량이 서로 같고, vitellogenin이 간에서 합성되어 난소내로 이동하는 점으로 보아, 난소내 난황단백 성분의 전구물질은 혈청내의 vitellogenin인 것으로 추정되었다.

• 동의생리병리학회지
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• 제19권2호
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• pp.481-489
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• 2005

Lonicerae Flos has antibacterial effects against Staphylococcus aureus, streptococci, pneumococci, Bacillus dysenterii, Salmonella typhi, and paratyphoid. It is an antiviral agent. The herb has a cytoprotective effect against $CCl_{4}-induced$ hepatic injury. It has antilipemic action, interfering with lipid absorption from the gut. Nowadays this herb is used mainly in the treatment of upper respiratory infections, such as tonsillitis and acute laryngitis. It is also used in the treatment of skin suppurations, such as carbuncles, and to treat viral conjunctivitis, influenza, pneumonia, and mastitis. Lonicerae Flos is dried flower buds of Lonicera japonica, L. hypoglauca, L. confusa, or L. dasystyla. But, for the most part, we use whole plant of Lonicera japonica, as a flower bud of it. And, little is known of the original copy of effects of whole plant, except for the 'Bon-Cho-Gang-Mok', which is written the effects of flower of Lonicera japonica are equal to effects of leaves and branch of it. The present study was conducted to evaluate the effect of flower and whole plant of Lonicera japonica on the regulatory mechanism of cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, flower and whole plant of Lonicera japonica water extracts inhibited nitric oxide production in a dose-dependent manner and abrogated iNOS and COX-2. Flower and whole plant of Lonicera japonica water extract did not affect on cell viability. To investigate the mechanism by which flower and whole plant of Lonicera japonica water extract inhibits iNOS and COX-2 gene expression, we examined the on phosphorylation of inhibitor ${\kappa}B{\alpha}$ and assessed production of $TNF-{\alpha}$, $interleukin-1{\beta}$ $(IL-1{\beta})$ and interleukin-6 (IL-6). Results provided evidence that flower and whole plant of Lonicera japonica inhibited the production of $IL-1{\beta}$, IL-6 and activated the phosphorylation of inhibitor ${\kappa}B{\alpha}$ in Raw 264.7 cells activated with LPS. These findings suggest that flower and whole plant of Lonicera japonica can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections, respectively.