• Tuberculosis and Respiratory Diseases
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• 제81권3호
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• pp.222-227
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• 2018

Background: Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea. Methods: Drug-resistant mycobacterial isolates were cultured to determine their susceptibility to anti-tubercular agents. Mutations in rpoC were identified by sequencing and compared with the relevant wild-type DNA sequence. Results: In total, 93 M. tuberculosis clinical isolates were successfully cultured and tested for drug susceptibilities. They included 75 drug-resistant tuberculosis species, of which 66 were RFP-resistant strains. rpoC mutations were found in 24 of the 66 RFP-resistant isolates (36.4%). Fifteen different types of mutations, including single mutations (22/24, 91.7%) and multiple mutations (2/24, 8.3%), were identified, and 12 of these mutations are reported for the first time in this study. The most frequent mutation involved a substitution at codon 452 (nt 1356) resulting in amino acid change F452L. Conclusion: Fifteen different types of mutations were identified and were predominantly single-nucleotide substitutions (91.7%). Mutations were found only in dual isoniazid- and RFP-resistant isolates of M. tuberculosis. No mutations were identified in any of the drug-susceptible strains.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1178-1184
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• 2018

The spread of antibiotic-resistant Enterobacteriaceae in the community is one of the main challenges for antibiotic treatment of community-onset infections. We evaluated the microbiological and molecular characteristics of stool samples from adults with comprehensive health examinations. Of 109 fecal samples, bacterial growth was observed in 86 samples and 61 gram-negative bacterial isolates were identified, of which 45 were Escherichia coli isolates (73.8%). Two isolates of Raoultella showed imipenem resistance, and both E. coli and Citrobacter freundii showed intermediate resistance to imipenem. Colistin resistance was identified in isolates of Klebsiella variicola and Salmonella subterranean, but no isolates carried mcr-1. As for E. coli genotypes, 35 sequence types were identified. $bla_{TEM-1}$, $bla_{TEM-30}$, and $bla_{CTX-M}$ were identified in 15, 1, and 4 E. coli isolates, respectively. In addition, all four Klebsiella pneumoniae isolates carried $bla_{SHV}$. Many genotypes that have been identified in isolates causing human infections were found in isolates in this study. There is a need to control the rise and spread of antibiotic-resistant pathogens by fecal carriage.

• 농촌계획
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• 제7권2호
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• pp.33-40
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• 2001

The purpose of this paper is on identifying spatial structure of Palwang and Taechon village and dealing with restoration direction of village spatial structure and village groves. The results are as follows. It was identified that the standing stone for penis form is not standing stone for praying son but complementing that for enforcing enclosed function of enclosing village groves. It was considered that village entrance and entrance road, village grove of Taechon village must be restored for being identified as standing stone of penis form of spatial structure of village. It was considered that the above is essential to connect with ecotourism of Sunchang-gun region. It was identified that Palwang village grove is very good in conservation state, but Taechon village grove has been destructed mostly by various development. It was identified that Palwang village grove is high in species diversity and in scare value as mixed forest, landscape value. The dominent tree of Palwang village was Pinus densiflora and that of Taechon was Salix glandulosa. Salix glandulosa was used pavilion tree, main tree in both village. It was identified that Carpinus tschonoskii is showing the most rapid growth in tree height and Salix glandulosa is showing the most rapid growth in tree breadth diameter of all kind of tree. It was considered that Pinus densiflora was planted first of all and deciduous trees were complemented later. It was considered that simple forest of Pinus densiflora has been changed into mixed forest by artificial affect in the long term. It was considered that Salix glandulosa in Palwang village grove must be indicated as legal protection tree according to present criteria of protection tree selection in Korea. And it is considered desirable that standing stone of penis form and village grove in Palwang village must be indicated unitedly as cultural conservation element.

• 패션비즈니스
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• 제24권4호
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• pp.30-47
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• 2020

The purpose of this study is to derive the type of image pursued by men at work and to present the fashion style element as a method of expressing the image to be pursued. The was carried out by deriving the types of seeking images of men at work through literature research and questionnaire on seeking images and analyzing cases through FGI. The results of the study on fashion style elements for each type of image pursued are as follows. First, the reliable image appeared a gentle and formal fashion style, and colors and items were identified as the main elements of expression. Second, the refined image appeared in a chic and dandy fashion style, and colors, fabrics and items reflecting trends were identified as the main elements of expression. Third, the neat image was identified as a simple and minimal fashion style, and details, fabrics, and colors were identified as the main elements of expression. Fourth, the luxurious image was found to have an intellectual feeling in a classic fashion style, and color and material are the main elements of expression. Fifth, the dignified image appeared in a fashion style that was well-dressed and felt classy and heavy, and colors, patterns, and items were identified as the main elements of expression. Sixth, the energetic image emerged as a business casual style, except sporty or active functional styles, with overall comfortable and cheerful feeling, fit, color, and items were noted as the main elements of expression.

• 한국임상수의학회지
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• 제18권3호
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• pp.243-248
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• 2001

Radiographic findings of degenerative joint disease (DJD) in the coxofemoral joints were studied in 108 dogs referred to Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University. Radiographic findings were identified with a extended ventrodorsal projection. Clinical signs such as pain in the affected joint, exercise intolerance, lameness, crepitation on flexion and extension of the joint and decreased range of motion, were shown in 40% and radiographic changes of bilateral coxofemoral joints were identified in 69% of a group of dogs. There are no sex predilections. Thirty-seven dogs were diagnosed as canine hip dysplasia (CHD; 10 cases), avascular necrosis of the femoral head (Legg-Calves-Perthes disease [LCPD]; 12 cases), fracture of the acetabulum (1 case), luxation(10 cases) and subluxation (15 cases), however, luxation and subluxation were identified in 11 dogs affected with CHD and LCPD. Causes of DJD in others (71case) were not identified. Radio-graphic findings identified included osteophyte formation at the attachment of the joint capsule (70%), sclerosis of the subchondral bone of the cranial acetabular edge (47%), remodeling of the femoral head (34%), thickening of the femoral neck(31%), perichondral osteophyte formation (31%), joint laxity (30%) and so on. All of the dogs diagnosed as CHD were large breed such as Retriever, Germain Shepherd, and Weimaraner and their age was under 1 year. This condition occurred bilaterally in 70% of affected animals. Dogs diagnosed as LCPD were young (under 1 year), small breed dogs. The incidence of LCPD is greater in Yorkshire Terrier, Miniature Pinscher and Poodle. This condition occurred unilaterally in 67% of affected animals. Clinical signs of dogs with unknown cause were shown in 15% and this condition occurred bilaterally in 75% of affected animals. Radiographic findings varied with patient's age and breed, however, the most common finding was osteophyte formation at the attachment of the joint capsule.

• 약학회지
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• 제40권3호
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• pp.251-261
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• 1996

Acanthopanax divaricatus Seem. is a medicinal plant growing widely through out Korea, Japan and China. The plant material of Acanthopanax spp. is used as analgesic , tonic, sedative drug as well as for the treatment of hypertension, rheumatism and diabetes. From the stem barks and root barks of A. divaricatus, diterpenoid compound was isolated and identified as pamaric acid ($C_{20}H_{30}O_2,\;mp\;164^{\circ}C$), lignan compounds were isolated and identified as d-sesamin ($C_{20}H_{18}O_6,\;mp\;123{\sim}124^{\circ}C$), eletheroside E ($C_{34}H_{46}O_{18},\;mp\;257{\sim}259^{\circ}C$), three sterol compounds were identified as ${\beta}$-sitosterol, campesterol, stigmasterol, and six fatty acid compounds were identified as stearic acid, palmitic acid, oleic acid, linolenic acid, arachidonic acid and behenic acid. And also leaves of A. divaricatus, chiisanoside were identified, one of secotriterpenoidal compound(white amorphorous powder crystal, mp $228^{\circ}C$). Anticancer activity and nephrotoxicity were tested by MTT assay. Anticancer effect of chiisanoside was much lower than that of cisplatin.

• 대한영양사협회학술지
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• 제24권2호
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• pp.159-168
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• 2018

Children's exposure to various vegetables has been associated with higher preferences and intake of vegetables. Attractive names have been reported to increase children's vegetable intake in school lunches. This study hypothesized that the number of vegetables identified correctly may be used as a measure of exposure to vegetables. The survey was administered to students (n=368) at an elementary school located in Gyeonggi-do to determine the association of number of vegetables identified correctly with factors regarding vegetable intakes and effects of descriptive menu names on consumption intentions in school lunches. Students were asked to identify the correct names for nine vegetables and rate their desire to eat six vegetable dishes labeled with general names and two different descriptive names. Descriptive menu names did not consistently increase subjects' desire to eat vegetables compared to general menu labels. Children who ate two vegetable dishes and more per meal tended to identify the names of more vegetables than those who ate less, without reaching statistical significance (P=0.057). Moreover, 5th graders who answered that they ate more than two vegetable dishes each meal had significantly higher scores in identifying vegetable names than those who did rarely (P<0.05). There was no significant association between the number of correctly identified vegetable names and children's vegetable preferences and frequency of being served vegetable dishes. Mother's vegetable preference showed a significant positive association with vegetable preferences of children (r=0.147, P<0.01) and fathers (r=0.340, P<0.01) as well as the number of correctly identified vegetables by children (r=0.183, P<0.01). The results of this study cannot support the advantage of descriptive menu labels over general menu labels. However, it suggests that the number of correctly identified vegetable names could serve as a measure of children's exposure to vegetables associated with factors regarding vegetable intake.

• 한국균학회지
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• 제37권2호
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• pp.121-129
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• 2009

In 2007, a total of 77 isolates of Fusarium spp. were obtained from ear rot symptoms of corns collected from 5 locations in Gangwon Province, Korea. The fungal isolates were identified based on their morphological features. Out of the isolates, fifteen isolates were identified as Fusarium verticillioides which formed microconidia in long chains on monophialides. Four isolates were identified as F. subglutinans which formed microconida only on false heads. Six isolates were identified as F. graminearum which produced red pigment in PDA culture. Besides these Fusarium species, F. napiform, F. nygamai, and F. oxysporum were identified from the rest isolates. To assess for genetic diversity of the isolates, a random amplified polymorphic DNA(RAPD) technique was carried out using URP primers. The results from the RAPD analysis showed that the isolates from corn were divided into 6 groups. These RAPD groups of the Fusarium species corresponded to morphological characters of the Fusarium species. The phylogenetic analysis of most isolates by DNA sequencing of EF-1$\alpha$ gene corresponded to morphological characters of the Fusarium species. The results of pathogenicity tests by two inoculation methods revealed that F. verticillioides, F. graminearum and F. subglutinans are strongly pathogenic to corn stalks.

• 한국의류학회지
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• 제42권3호
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• pp.530-543
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• 2018

This study investigated elderly winter wearing habits and self-identified thermal tolerance in order to compare differences between older males and older females. We surveyed 269 participants: 119 males (age $76.9{\pm}5.9yr$) and 150 females (age $76.1{\pm}5.7yr$). Thirty one questionnaires were used: Self-identified cold and heat tolerance, clothing behavior, lifestyles related to the cold and health care, etc. High percentage of respondents (both males and females) felt vulnerable to the cold. The total number of outdoor clothes were $9.6{\pm}1.6$ and $10.6{\pm}1.6$ in males and females, respectively (p<.05). Wearing frequencies for thermal underwear were 84.0% and 82.4% in males and females, respectively. The using percentage of auxiliary heating devices was higher in females compared to males (p<.01). Males felt more cold in the head and face compared to females (p<.01). Self-identified cold tolerance had negative relationships with the total number of winter outdoor clothes for both males (r=-0.260) and females (r=-0.222) (p<.01). The perceived old age for both males and females was 72.4 yr. Sex differences should be considered when developing guidelines for winter clothing behavior for the elderly and educating them on how to improve health and cold tolerance.

• 한국정밀공학회지
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• 제17권8호
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• pp.78-86
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• 2000

This study identified a linear time-invariant mathematical model of each stand of a five-stand tandem cold mill. Two model identification methods are applied to construct a linear model of each stand of the tandem cold mill. For the model identification the input-output data that have interstand interference property in tandem cold rolling are obtained from a nonlinear simulator of the tandem cold mill. And a linear model of each stand is identified with N4SD(numerical algorithms for subspace state space system identification) method based on a state-space model and Least Square algorithm based on a transfer function. Furthermore a modeling error of the tandem cold mill is quantitatively analyzed from a maximum singular value plot of error function between an identified nominal model and uncertain model. In conclusion the comparison of the output signals between the existing Taylor linearized model the identified linear model and the nonlinear model of the tandem cold mill shows the accuracy and the applicability of the proposed identified model.