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Comparative Study for Compatibility of Acellular Dermis ($SureDerm^{TM}$) and Synthetic Material (PROCEED$^{(R)}$) on Abdominal Wall Defect in Rabbit Models (토끼의 전층 복벽 결손 재건에서 무세포진피 ($SureDerm^{TM}$)와 합성물질(PROCEED$^{(R)}$)에 대한 생체적합성 비교 연구)

  • Kang, Nak-Heon;Song, Seung-Han;Kang, Dae-Young;Ahn, Jae-Hyoung;Choi, Da-Mi;Kim, Jin-Young
    • Archives of Plastic Surgery
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    • v.38 no.2
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    • pp.135-142
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    • 2011
  • Purpose: Deficiencies of the abdominal wall can be the a result of infection, surgery, trauma, or primary herniation. For abdominal wall reconstruction, synthetic materials have been shown to provide a better long-term success rate than primary fascial repair. But, synthetic materials cannot elicit angiogenesis or produce growth factor and are therefore plagued by an inability to clear infection. As a result of the inherent drawbacks of synthetic, significant effort has been spent on the identification of new bioprosthetic materials. The aim of our study is to evaluate the effectiveness of a synthetic material (PROCEED$^{(R)}$) and an ADM ($SureDerm^{TM}$) to repair abdominal wall defects in a rabbit models. Methods: We measured the tensile strength of the $SureDerm^{TM}$ and PROCEED$^{(R)}$ by a Tension meter (Instron 4482). 16 Rabbit models were assigned to this study for abdominal wall reconstruction. Abdominal defect of 8 rabbits were reconstructed by PROCEED$^{(R)}$ and the rest were reconstructed by $SureDerm^{TM}$. We assessed gross and histologic examinations for the reconstructed abdominal wall. Results: The tensile strength of $SureDerm^{TM}$ and Gore Tex$^{(R)}$ is $14.64{\pm}0.51Mpa$, $8.54{\pm}0.45Mpa$. PROCEED$^{(R)}$ was estimated above the limits of measurement. Inflammatory reaction of PROCEED$^{(R)}$ persisted for 32weeks, but $SureDerm^{TM}$ decreased after 16weeks. Vascular ingrowth into the $SureDerm^{TM}$ was seen after 32 weeks. The basement membrane of $SureDerm^{TM}$ changed into a form of pseudoperitoneum. In PROCEED$^{(R)}$, it seemed like pseudoepithelial lining was made from the fibrosis around the mesh. Conclusion: In our study, the $SureDerm^{TM}$ not only have less inflammatory reaction and presented more angiogenesis than the PROCEED$^{(R)}$, but also have pseudoperitoneum formation. It is expected that $SureDerm^{TM}$ is useful for abdominal wall reconstruction. However, a long-term study of its usage consequences are thought to be needed.

Comparative Study of Acute Dyspepsia Patients Depending on Stress Factor and Food Factor (과심상(過心傷), 음식상(飮食傷)에 따른 급성 소화불량증 환자간의 비교 연구)

  • Kim, Hyo-Jin;Lee, Soo-Jung;Lee, Ah-Ram;Kang, Kyung-Hwa;Kim, Won-Il
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.3
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    • pp.525-532
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    • 2010
  • Dypepsia is the most common gastrointestinal disease and mainly occurs by stress or food. According to the cause of dyspepsia, the clinical characteristics and the curative process are different in patients. The purpose of this study is to compare acute dyspepsia patients depending on food factor and stress factor. For this study, we analyzed the cause, and the symptoms of 59 acute dyspepsia patients and divided them into two groups: one group is 27 acute dyspepsia patient group caused by stress factor (stress factor group). The other group is 32 acute dyspepsia patient group caused by food factor (food factor group). In addition, we carried out HRV test and compared HRV index between two groups. There was no significant difference in past illness involved in gastrointestinal tract diseases between two groups. There were big differences in the frequency of chest discomfort, thurst, lack of appetite, and exhaustion after meals between two groups. There were many changes of tongue fur in food factor group. But there were many changes of tongue body in stress factor group. The pulse pattern is mostly wiry in stress factor group. But, The pulse pattern is mostly slippery in food factor group. HF, TP were significantly lower than food factor group in stress factor group, it means autonomic nerve system was more suppressd in stress factor group than food factor group. Food factor group improved sooner than stress factor group. This study presented the clinically different characteristics (past medical history, symptoms, tongue diagnosis, pulse pattern, curative process) of acute dyspepsia depending on food factor and stress factor. Thus, it provides the necessity of oriental medical diagnosis and treatment called pattern identification for acute dyspepsia.

Comparative Study on Exposure Factors for Risk Assessment in Contaminated Lands and Proposed Exposure Factors in Korea (토양오염 위해성평가를 위한 국가별 노출인자 비교분석 및 국내 노출인자 연구)

  • An, Youn-Joo;Lee, Woo-Mi
    • Journal of Soil and Groundwater Environment
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    • v.12 no.1
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    • pp.64-72
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    • 2007
  • Humans are exposed by a range of pollutants in soil via exposure routes such as ingestion, inhalation, and dermal contact. Risk assessment is a process of evaluating the adverse health effects of chemicals as a result of exposure to stressors, and it is a very useful tool to establish the cleanup goals in contaminated lands. In the exposure assessment that is one of main process in risk assessment, exposure factor plays a significant role to quantify the intake of soil pollutants. However there is a very limited study about the exposure factor applicable to Korea. In this study, we compared the exposure factors applied by the developed countries including the United States and representative European countries, and suggested the exposure factor that might be suitable in our situation. The exposure factors considered in this study include average lifetime, body weight, (exposed) skin surface area, life time, skin absorption, soil-skin adherence factor, and soil ingestion rate. This information is needed to quantitatively estimate the intake of soil pollutants in contaminated lands.

Isolation of Enterovirus Causing Aseptic Meningitis in Busan, 2000-2002 Years (2000-2002년 바이러스성 뇌수막염의 발생양상 및 특성)

  • Jo, Gyeong-Sun;Jeong, Myeong-Ju
    • Korean Journal of Microbiology
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    • v.39 no.4
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    • pp.248-252
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    • 2003
  • Enteroviruses isolation were attempted from samples obtained from aseptic meningitis-suspected patients in hospitals in Busan during 2000-2002. Enteroviruses were found in 2 of 292 cases in 2000, 4 of 371 cases in 2001, 83 of 703 cases in 2002. In 2000, the isolated viruses were found to be echovirus serotype 11 and coxsackievirus serotype B2. Coxsackievirus serotype B5 was isolated in 2001 and in 2002, echovirus serotypes 2, 3, 6, 7, 9, 13, 25, 30 were isolated in 70 cases while coxsackievirus serotypes B3 and B4 were isolated in 10 cases. Various specimens tended to emerge over the years. The occurrence in 2000 tended to be mostly focus during the cold months, December through January, while in 2001, it occurred in May. In 2002, occurrence was found to be distributed from April to November with the highest rate during June and July. The strains of Vero and HEp-2 of echovirus and coxsackievirus, respectively, are highly infectious. Electron micrograph of echovirus and coxsackievirus show that they are small nonenevolped, isometric-shaped viruses. Isolated RNA from strains of echovirus and coxsackievirus showing cytopathic effects were used to undergo nested PCR which resulted in a 436 bp single band in all the strains. The serotype was sent to the Department of Virology at the Korean National Institute of Health for identification.

Nutrition Knowledge, Dietary Attitudes, Dietary Habits and Awareness of Food-Nutrition Labelling by Girl's High School Students (여고생의 영양지식, 식태도, 식습관 및 식품영양표시에 대한 인식)

  • Cho, Su-Hee;Yu, Hyeon-Hee
    • Korean Journal of Community Nutrition
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    • v.12 no.5
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    • pp.519-533
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    • 2007
  • This study was carried out to investigate the nutrition knowledge, dietary attitudes, and dietary habits of girl's high school students in Kunsan, and to investigate their recognition of food-nutrition labeling. The results are summarized as follows. General nutrition knowledge is relatively low, with an average of 0.57. It was shown that high school female students skipped breakfast rather than lunch or dinner, with a skipping rate of 28.9% for breakfast, 0.7% for lunch, and 8.6% for dinner. Regarding snacks, 35.5% of all the surveyed students had 1 snack per day, with 31.9% having them between lunch and dinner. The most popular snacks include biscuits (22.3%), noodles (18.3%) and bakery (13.3%). The most popular response was that students 'sometimes checked' the food label of processed domestic and imported processed food. The level of satisfaction with food labels is moderate, with an average of 2.96, out of 5. The most satisfactory title about food labels was 'helpful for food selection' with 3.19. On the other hand, the least satisfactory title was 'understands the label' with 2.78. Regarding the identification of the nutrition labeling, the highest response was 'sometimes watched, sometimes not' with 40.5%. Products which were most often checked were milk/milk products (3.44), snacks/bread (3.33), and soft drinks (3.07). Among nutrition labeling items, total calories was the most important, followed by fat, carbohydrate, cholesterol and calcium. The question regarding the knowledge of nutrition labeling rated an average of 0.58 (out of 1). There was a significant positive correlation between the degree of the nutrition label verification and the dietary attitude score, along with the nutrition labeling knowledge and the nutrition knowledge score of the subjects. On the other hand, the degree of the nutrition label verification and the knowledge on nutrition labeling had a significant negative correlation. Hence, it is of the opinion that education on properly reading nutrient information is necessary to enable adolescents to apply that in real life. Furthermore, labeling nutritional information on processed fred through a more comprehensive method is deemed necessary as a supporting measure.

Isolation and Characterization of Colletotrichum Isolates Causing Anthracnose of Japanese Plum Fruit (자두 탄저병균의 분리 및 동정)

  • Lee, Yong-Se;Ha, Da-Hee;Lee, Tae-Yi;Park, Min-Jung;Chung, Jong-Bae;Jeong, Byeong-Ryong
    • Korean Journal of Environmental Agriculture
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    • v.36 no.4
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    • pp.299-305
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    • 2017
  • BACKGROUND: Although the filamentous fungal pathogen Colletotrichum species causing anthracnose disease on various fruits including peach, apple, persimmon and grape, there is no report on Japanese plum in Korea. METHODS AND RESULTS: In 2016, diseased fruits showing typical anthracnose symptoms of Japanese plum were collected in market and ochards. Diseased tissue was cut off and disinfected subsequently with 70% ethanol for 1 min, and in 1% sodium hypochloride solution for 1 min, followed by three washes with sterile distilled water. The disinfected tissues were placed onto potato dextrose agar (PDA), and incubated at $25^{\circ}C$ in the dark for 5 to 7 days. For single-spore isolation, conidia were scraped off the plate using a loop, and suspended with 10 mL sterile distilled water. One hundred microliter of the conidial suspension was spread on PDA plates and incubated at $25^{\circ}C$. Finally, one germinated conidium was transferred onto PDA plates. Morphological and cultural characteries of colonies and spores of isolated Colletotrichum were observed after 7 to 10 days incubation on PDA. Molecular identification of isolates were analyzed by comparing rDNA-ITS gene sequences with NCBI GeneBank. CONCLUSION: Of eleven isolates of Colletotrichum isolated from anthracnose diseased Japanese plum fruits, six were identified as C. acutatum, and five as C. gloeosporioides based on diagnostic characteristics such as colony growth rate, shape and size of conidia, and rDNA-ITS sequences. This is the first report of Colletotrichum causing the anthracnose on Japanese plum in Korea.

Price Response Function With and Without Choice Set Information in Denim Jeans Market (고려상품군의 유.무에 따른 가격반응함수의 비교연구)

  • Kwak, Youngsik;Lee, Jin-Hwa
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.910
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    • pp.1273-1281
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    • 2004
  • The primary purpose of this study is to suggest a new methodology for calibration of a continuous price response function and to compare the differences in the price response function with and without choice set information. Through the new methodology, the two-staged conjoint analysis, the continuous price response function far jeans market was calibrated. Three steps were required to complete the two-staged conjoint analysis. Step one provided respondents with both a written and a visual description of two different randomly selected styles and colors of denim jeans. In step two respondents were asked to choose the combination of attributes they intended to purchase. Based upon the literature review, these four attributes included: brand, style, price, and color. Respondents were required to assess their purchase intentions for 32 combinations by marking Yes if she/he would purchase a given combination and No if she/he would not purchase a given combination. This allowed for identification of each respondents choice set. Instructions in step three required respondents to rate each combination marked Yes on a scale of 1-100, with one as least likely to be purchased and 100 as most likely to be purchased. This value served as the dependent variable for estimating the parameters in the model. Furthermore, the empirical study shows that there is a difference in price response function with and without choice set information. Therefore, when one calibrates a price response function far a given brand, we can recommend to include choice set information in his/her research.

Identification of process generating formaldehyde in a furniture manufacturer (특정 가구 제조 공장의 포름알데히드 발생 공정 노출 평가)

  • Yoo, Kye-Mook;Lee, Mi-Young
    • Analytical Science and Technology
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    • v.27 no.5
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    • pp.243-247
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    • 2014
  • Formaldehyde is defined as carcinogen causing leukaemia, lymphoma or nasopharyngeal carcinoma at high level of exposure. Furniture-manufacturing workers can be exposed to formaldehyde, which implies serious impact on health of the workers. The authors carried out ambient monitoring of formaldehyde in the field, and identified the source of formaldehyde generated during the working process by testing the condition in the laboratory settings. After sampling formaldehyde in the air with 2,4-DNPH (2,4-dinitrophenylhydrazine) coated silica gel, we extracted formaldehyde derivative with acetonitrile and analyzed the extract using HPLC with UV detector at 360 nm. Formaldehyde was separated by ACQUITY UPLC BEH $C_{18}$ column at a flow rate of 0.5 mL/min using 45% acetonitrile as mobile phase. The workers were exposed to higher level of formaldehyde than normal air. Formaldehyde up to 0.31 ppm was detected in the process of veneer attachment, which exceeded 0.3 ppm, the ceiling value of ACGIH standard. The laboratory test of measuring formaldehyde generated from the glue and veneer used in the attachment process resulted in more formaldehyde generation as the temperature increased, and more from the veneer. Heating the veneer to $100-150^{\circ}C$ following the real condition of the manufacturing site generated 1.14-2.70 ppm of formaldehyde from the sample, which was 2-5 times higher level than Korean limit of exposure (0.5 ppm). As the workers handling and processing the veneer which was produced by wet process had high possibility to be exposed to formaldehyde, urgent improvement and management of working environment of furniture manufacturer is demanded.

A STUDY OF MONOMER RELEASE FROM PIT AND FISSURE SEALANTS ACCORDING TO VARIOUS LIGHT SOURCES (광원에 따른 수종의 치면열구전색제로부터 용리되는 모노머에 관한 연구)

  • Seo, Hyun-Woo;Park, Ho-Won
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.2
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    • pp.284-292
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    • 2005
  • The aim of this study was to identify and quantify the major or detectable monomers released from any of five commercially-available, light-cured pit and fissure sealants with three different light sources : conventional halogen light curing unit, plasma arc light curing unit and LED curing unit. After curing, specimens were immediately immersed in distilled water for different time intervals. The time related release of monomers were analyzed by high performance liquid chromatography(HPLC). Identification and quantitative analysis of monomers were performed by the comparison of the elution time and the absorption peak height of the eluates with those of the authentic sample. The result of this study can be summarized as follows. 1. Standard solution peaks with retention times of 2.3, 3.2, 5.6, 6.5, 10.4 minutes were identified as BPA, TEGDMA, UDMA, Bis-GMA, Bis-DMA, respectively. 2. None of the chromatograms of the tested sealants displayed peaks with the same retention time as that of the standard solution, except for TEGDMA. 3. The highest release rate of TEGDMA was observed during the 12hr period for all samples and declined thereafter. 4. The elution of TEGDMA from curing with Halogen curing unit for 20 second and LED for 10 second was less than that resulting from curing with Plasma arc for 3 second. 5. TEGDMA was detected at much lower levels in eluates from the Pit & Fissure $Sealant^{TM}$ than other sealants. The elution of TEGDMA from the $Helioseal^{(R)}$ F cured with Halogen light curing unit, the $Concise^{TM}$ cured with Plasma arc curing unit and the $Teethmate^{(R)}$ F-1 cured with LED curing unit were higher than other sealants.

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Analysis on PD Pulse Distribution by Defects Depending on SF6 Pressure (SF6 압력에 따른 결함별 부분 방전 펄스의 분포 분석)

  • Kim, Sun-Jae;Jo, Hyang-Eun;Jeong, Gi-Woo;Kil, Gyung-Suk;Kim, Sung-Wook
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.1
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    • pp.40-45
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    • 2015
  • Electrode systems: a protrusion on conductor (POC), a protrusion on enclosure (POE), a crack in epoxy plate and a free particle (FP) were fabricated to simulate insulation defects in a gas insulated switchgear (GIS). $SF_6$ gas was filled in the electrode systems by 3 bar and/or 5 bar, respectively. Partial discharge (PD) pulses were detected through a $50{\Omega}$ non-inductive resistor. A calibration test was carried out according to IEC 60270, and the sensitivity was 0.25 pC/mV. PD pulses were distributed in the phase of $50^{\circ}{\sim}135^{\circ}$ and over 95% of them existed in the phase of $55^{\circ}{\sim}120^{\circ}$ for the POC. PD pulses were distributed in the phase of $230^{\circ}{\sim}310^{\circ}$ and over 90% of them existed in phase of $220^{\circ}{\sim}300^{\circ}$ for the POE. PD pulses occurred in the phase of $40^{\circ}{\sim}60^{\circ}$ and $220^{\circ}{\sim}300^{\circ}$ for the crack, and pulse counts were 25% higher in negative polarity than in positive polarity. PD pulses were distributed in every phase unlike to other three electrode systems and the peak magnitude was measured at $118^{\circ}$ and $260^{\circ}$ for the FP. As described above, PD pulses were observed in positive polarity for the POC, in negative one for the POE, in both one for the crack and the FP. In conclusion, it is expected that the identification rate of defect type can be improved by considering the polarity ratio of PD pulses on the PRPDA method.