• 한방안이비인후피부과학회지
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• 제28권1호
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• pp.134-142
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• 2015

Objective : This study was carried out to find out the effect of Korean medical treatments on tinea pedis with id reaction. Method : We treated a 33-year-old man was afflicted with tinea pedis complicating id reaction with herbal medicine, acupuncture, ointment, wet-dressing and steam treatment. After treatments, we measured the status of scales, fissures and the degree of pruritus. We followed up symptom changes by taking pictures and compared before and after. Result & Conclusion : Three days after admission, erythema occurred at upper and lower limbs and the degree of pruritus increased by id reaction. As tinea pedis improved, id reaction decreased, too. Consequently, both sites of primary lesions of tinea pedis and secondary id reactions were significantly improved, and accompanied symptoms were resolved simultaneously.

• 한국동물위생학회지
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• 제17권2호
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• pp.95-113
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• 1994

To establish an effective diagnostic measure for detection of the antibodies against Actinobacillus pleuropneumoniae, the methods for tube agglutination test (TAT), plate agglutination test (PAT), micro-agglutination test(MAT) and agar-gel immunodiffusion test(ID) were improved and standarized, and the comparative studies were carried out. The results obtained through the experiments were summarized as follows. 1. The rabbit hyperimmune sera to reference serotypes 1 to 6 were cross-tested with TAT, PAT, MAT and ID. In the homologous systems, the range of antibody titers in TAT was 80 to 640, showing the cross-reaction in serotypes 3, 4, 5 and 6. The range of antibody titers in PAT was 4 to 64, showing the cross-reaction in serotypes 3, 4, 5 and 6. In ID, the range of antigen titers was 8 to 32, and cross-reaction was observed in serotype 5. 2. The optimal concentration of antigen in PAT and MAT were 100mg /ml and 1.25mg /ml respectively. The most sensitive reaction in MAT was observed in 52$^{\circ}C$ for 18hrs. 3. In ID, the most promising antigen and the buffer for agar-gel were EDTA-treated antigen and 0.05M tris buffer (pH 7.2), respectively. 4. By the tests for 200 swine sera, it was found that the frequency of positive reaction were 203 in TAT, 240 in PAT and 163 in ID. 5. When compared the titers of TAT with those of MAT for 200 swine sera, MAT showed the higher titer than TAT being increased by relative correlation. Int was found that the titer for positive readings were 20 in TAT and 40 in MAT. 6. when compared the results of ID with those of TAT for 200 swine sera, all sera with TAT titer under 10 were negative in ID. Of the sera with TAT titer 20 and 40, 55.1% nd 91.8% were positive in ID, respectively. All sera with TAT titer above 80 were positive in ID. In comparison of ID and MAT, all sera with MAT titer under 20 were negative in ID. Of the sera with MAT titer 40 and 80, 24.7% and 93.9% were positive in ID, respectively. All sera with MAT titer over 160 showed positive in ID. 7. In conclusion, the established MAT showed high sensitivity but low specificity, wherease ID revealed low sensitivity but high specificity.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.308-313
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• 2008

Ixeris dentata (ID, family Asteraceae), called Seumbakuy in Korea, was fermented with lactic acid bacteria (LAB) and their antiallergic activities were investigated. Fermentation of ID with Bifidobacterium breve or Lactobacillus acidophilus increased its inhibition of degranulation in RBL-2H3 cells induced by the IgE-antigen complex. Oral administration of these extracts to mice inhibited the passive cutaneous anaphylaxis (PCA) reaction induced by the IgE-antigen complex and scratching behaviors induced by compound 48/80. The fermented ID more potently inhibited the PCA reaction and scratching behaviors than the non-fermented one. These extracts also inhibited mRNA expression of TNF-${\alpha}$ and IL-4, as well as NF-${\kappa}B$ activation in RBL-2H3 cells induced by the IgE-antigen complex. These findings suggest that LAB fermentation improves ID-mediated inhibition of IgE-induced allergic diseases such as rhinitis and asthma, and that ID works by inhibiting degranulation and NF-${\kappa}B$ activation in mast cells and basophils.

• Journal of Yeungnam Medical Science
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• 제5권2호
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• pp.47-52
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• 1988

1988년 8월초부터 9월말동안 영남의료원 내과를 내원한 사람중 1304명에서 대변도말검사를 실시하였으며, 이중 443명에서는 간흡충증 피내반응검사를 하였고, 간흡충증이 의심되는 79명에 대해서는 집란법을 동시에 시행하여 간흡충증을 조사한 결과 다음과 같은 결과를 얻었다. 1) 간흡충 감염율은 3.8%였다. 2) 간흡충증 피내반응검사의 감수성은 82.1%(32/39명)이었고, 특이성은 64.6%(261/404명)이었다. 3) 도말검사상 음성이었으나 피내반응검사가 양성이면서 간흡충증이 의심되거나, 양 검사 모두 음성이나 말초 호산구성 세포의 증가로 간흡충증이 의심되는 사람 79명을 집란법으로 조사한 결과 79명중 16명에서 간흡충란이 발견되었다. 4) 도말검사상 음성이었으나 집란법으로 충란이 발견된 16명중 13명은 경감염이었고 3명은 중등감염된 것으로 나타났다. 이상의 결과에서 간흡충증이 의심되는 환자에게는 반드시 대변도말검사와 함께 피내반응검사를 실시하고 필요시 집란법으로 EPG와 EPD을 조사한 후 확진하여 치료하는 것이 바람직하리라 생각된다.

• 한국발생생물학회지:발생과생식
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• 제17권1호
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• pp.17-24
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• 2013

This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.

• Korean Chemical Engineering Research
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• 제56권5호
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• pp.738-751
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• 2018

The effect of the reaction temperature and reaction time on the thermal oxidative purification quality of detonation nanodiamond (NDsoot) was investigated in a gas-solid fluidized bed reactor of a $0.10m-ID{\times}1.0m$-high stainless steel column with zirconia beads ($d_{SV}=99.2{\mu}m$). The carbon conversion increased with increasing the reaction temperature; however, when the reaction temperature was greater than 773 K, the carbon conversion did not increase. The content of $sp^3$-hybridized carbon at the reaction temperature of 703 K barely changed when the reaction time was more than 30 minutes, but at 773 K, the content decreased as preferred. At 703 K, the purification quality increased with the increasing reaction time; however, at 773 K, the purification quality increased up to 30 minutes and then decreased rapidly.

• 기본간호학회지
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• 제18권1호
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• pp.46-53
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• 2011

Purpose: The purpose of this study was to identify the effects of EMLA cream (eutectic mixture of local anesthetics, lidocaine and prilocaine) on pain during ampicillin sodium intradermal (ID) skin test, and also to assess skin reaction after the skin test. Methods: Forty-three nurse-volunteers had skin tests with 0.01ml-0.05ml ampicillin sodium antibiotics. Skin tests were done on each forearm to compare the pain level of the skin test site after application of EMLA cream with the pain level when no EMLA cream was applied. EMLA cream was applied at the ID skin test site with an occlusive dressing for one hour. Pain was evaluated using a visual analogue scale and pain sensation using the short form McGill Pain Questionnaire. The transverse diameter of the wheal and redness was read right after and at 15 minutes after the skin test. The results were compared using independent t-tests. Results: Pain score and sensation with EMLA cream treatment were significantly lower than when EMLA cream was not applied. There was no difference in skin reactions; reading of the skin test was not affected by EMLA cream. Conclusions: EMLA cream was found to be an effective local anesthetic to relieve the pain of clients having ampicillin sodium antibiotics ID skin tests.

• Mass Spectrometry Letters
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• 제11권4호
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• pp.108-112
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• 2020

A certified reference material (CRM) for the analysis of nutrient elements in an edible mushroom (Ganoderma lyceum) powder has been developed (KRISS CRM 108-10-011). The mass fractions of calcium (Ca), iron (Fe), and zinc (Zn) were measured by isotope dilution inductively coupled plasma mass spectrometry (ID ICP/MS). To dissolve the fungi cell wall of mushroom consisted of chitin fibers, sample preparation method by single reaction chamber type microwave-assisted acid digestion with acid mixtures was optimized. The mean measurement results obtained from 12 sample bottles were used to assign as the certified values for the CRM and the between-bottle homogeneities were evaluated from the relative standard deviations. The certified values were metrologically traceable to the definition of the kilogram in the International System of Units (SI). This CRM is expected to be used for validation of analytical methods or quality control of measurement results in analytical laboratories when they determine the mass fractions of elements in mushroom or other similar samples.

• 대한화학회지
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• 제28권2호
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• pp.95-101
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• 1984

$cis - [Cr(en)_2Cl_2]^+ $착물의 수화반응에 대한 연구를 전기전도도법에 의해. 온도는 $15^{\circ}C{\sim}30^{\circ}C$에서 압력은 $1{\sim}2000bar$의 범위에서 실시하였다. 반응속도는 온도가 증가함에 따라 증가하고, 압력이 증가하면 감소하였다. 활성화부피(${\Delta}V^{\neq}$)는 $25^{\circ}C$, 1bar에서 $1.82cm^2/mole$로 비교적 작은 양의 값을 나타내었으며, 이 값은 압력이 증가함에 따라 감소하고 온도 증가에 따라서는 증가하였다. 활성화엔트로피(${\Delta}S^{\neq}$)$는 $25^{\circ}C$, 1bar에서 -9.019 eu로 작은 음의 값을 나타내었다. 활성화파라미터의 값으로부터 수화반응의 메카니즘은 교환해리(Id)메카니즘인 것으로 밝혀졌다.

• 대한핵의학회지
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• 제28권3호
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• pp.331-337
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• 1994

In order to develop a $^{18}F$-labelled myocardial perfusion agent(flow tracer) for PET, $^{18}F$-labelled organic ammonium cations were synthesized and evaluated in relation to their biodistribution. Five quaternary organic ammonium compounds were labelled with $^{18}F$ in a side chain with moderate to good yields by direct introduction of $^{18}F$-fluoride. Radiochemical yields have been achieved in 30-40min by the precursors (tosylates) in dimethylsulfoxide 15-60% (decay corrected). The reaction was found to be autocatalyzed. A remote controlled procedure was developed in these synthesis. $^{18}F$-Labelling and HPLC-purification of com-pounds needed about 60 min(Yield; 7-20%). Up to now the two compounds N-4-[$^{18}F$]fluorobutyl-pyridinium cation(1) and N, N dibenzyl-4(2-[$^{18}F$]fluoroethyl)piperidinium cation(2) were investigated in relation to their biodistribution in mice. Compound 1 showed at 1 min post injection the high uptake of 19.22% ID/g organ in the myocardium but a following fast decline to 1.12% ID/g organ after 40min. Uptake of compound 2 was after 1min in the heart 5.90% ID/g organ but after 40min at the relative high value of 4.33% ID/g organ. Heart:blood ratio for compound(1) at 1 min was 8.3, at 40 min 2.6 for compound II 2.0(1min) and 15.0(40 min). As data of compound 2 showed greater heart uptake, slower myocardial release, and higher heart: blood ratios, compound 2 is a good candidate for further evaluation.