• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3817-3824
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• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.1
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• pp.84-92
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• 2000

A GaAs MESFET MMIC transmitter for 2.4 GHz wireless local loop handset is designed and fabricated. The transmitter consists of a double balanced active mixer and a two stage driver amplifier with voltage negative feedback. In particular, a pair of CS-CG(common source-common gate) structure compensates the reduction in dynamic range caused by unbalanced complementary IF input signals. And to suppress the leakage local power at RF port, the mixer is designed by using phase characteristic between the ports of MESFET. At the bias condition of 2.7 V and 55.2 mA, the fabricated MMIC transmitter with chip dimensions of $0.75\times1.75 mm^2$ obtains a measured conversion gain of 38.6 dB, output $P_{idB}$ of 11.6 dBm, and IMD3 at -5 dBm RF output power of -31.3 dBc. This transmitter is well suited for WLL handset.

• Analytical Science and Technology
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• v.21 no.6
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• pp.502-509
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• 2008

An ID LC/MS (isotope dilution liquid chromatography/mass spectrometry) was used as a primary method for the quantitative analysis of cholesterol in human serum. The separation of cholesterol was carried out by Thermo ODS hypersil $C^{18}$ column. The mobile phase was 100% methanol, and flow rate was $0.3m{\ell}/min$. Cholesterol and cholesterol-$3,4-13C_2$ were monitored at m/z 369.4 and 371.3, which correspond to $[M-H_2O+H]^+$ respectively. In order to verify the measurement method, NIST SRM 909b was analyzed. The results agreed well with certified values within uncertainty. The four kinds of serum certified reference material were prepared and certified. The repeatabilities of measurement were ranged from 0.1 to 0.8% (RSD), which were relatively good. The reproducibility between independent measurement run was below 0.24% (RSD). The expanded uncertainty was about 1.43% within the 95% confidence interval.

• Korean Chemical Engineering Research
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• v.49 no.5
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• pp.582-587
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• 2011

Holdup characteristics of bubble, wake and continuous liquid phases were investigated in bubble columns with viscous liquid media. Effects of column diameter(0.051, 0.076, 0.102 and 0.152 m ID), gas velocity($U_G$=0.02~0.16 m/s) and liquid viscosity(${\mu}_L$=0.001~0.050 $Pa{\cdot}s$) of continuous liquid media on the holdups of bubble, wake and continuous liquid phases were discussed. The three phase such as bubble, wake and continuous liquid phases were classified successfully by adapting the dual electrical resistivity probe method. Compressed filtered air and water or aqueous solutions of CMC(Carboxy Methyl Cellulose) were used as a gas and a liquid phase, respectively. To detect the wake as well as bubble phases in the bubble column continuously, a data acquisition system(DT 2805 Lab Card) with personal computer was used. The analog signals obtained from the probe circuit were processed to produce the digital data, from which the wake phase was detected behind the multi-bubbles as well as single bubbles rising in the bubble columns. The holdup of bubble and wake phases decreased but that of continuous liquid media increased, with an increase in the column diameter or liquid viscosity. However, the holdup of bubble and wake phases increased but that of continuous media decreased with an increase in the gas velocity. The holdup ratio of wake to wake to bubble phase decreased with an increase in the column diameter or gas velocity, however, increased with an increase in the viscosity of con-tinuous liquid media. The holdups of bubble, wake and continuous liquid media could be correlated in terms of operating variables within this experimental conditions as: ${\varepsilon}_B=0.043D^{-0.18}U_G^{0.56}{\mu}_L^{-0.13}$, ${\varepsilon}_W=0.003D^{-0.85}U_G^{0.46}{\mu}_L^{-0.10}$, ${\varepsilon}_C=1.179D^{0.09}U_G^{-0.13}{\mu}_L^{0.04}$.

• Journal of KIISE:Software and Applications
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• v.36 no.7
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• pp.560-570
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• 2009

RFID, Radio Frequency Identification, is a technology of automated identification replacing bar-code. RFID technology has advantages that it recognizes fast and it is strong to contamination using wireless communication. However, there are difficult problems that should be solved for popularization of RFID. Among of these, tag anti-collision problem is dealed in this paper. It affected the performance of RFID system directly. This paper analyzes conventional algorithms and proposes new algorithms of tag anti-collision. The algorithm proposed was composed of appropriate properties to each phase of distribution and recognition as hybrid between ALOHA-based algorithm and QT-based algorithm. At phase of distribution, the number of tags recognizing at a frame was reduced using ALOHA-based algorithm. It addressed the delay problem because of deep depth of tree. At phase of recognition, it solved ALOHA-based chronic problem that couldn't recognize all the tags sometimes. Moreover, QTR algorithm that recognize by reversed tag IDs was adopted for the performance. The FQTR algorithm proposed in this paper showed brilliant performance as compared with convention algorithms by simulation.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.737-744
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• 2007

An isotope dilution-liquid chromatography/tandem mass spectrometric method was developed as a candidate reference method for the accurate determination of acrylamide in potato chips, starch-rich foodstuff cooked at high temperature. Sample was spiked with 13C3-acrylamide and then extracted with water. The extract was further cleaned up with an Oasis HLB solid-phase extraction (SPE) cartridge and an Oasis mixed-phase cation exchange (MCX) SPE cartridge. The extract was analyzed by using LC/ESI/Tandem MS in positive ion mode. LC with a medium reversed-phase (C4) column was optimized to obtain adequate chromatographic retention and separation of acrylamide. MS was operated to selectively monitor [M+H]+ ions of the analyte and its isotope analogue at m/z 72 and m/z 75, respectively. Sample was also analyzed by the LC/MS with selectively monitoring the collisionally induced dissociation channels of m/z 72 → m/z 55 and m/z 75 → 58. Compared to the LC/MS chromatograms, the LC/MS/MS chromatograms showed substantially reduced background chemical noises coming from solvent clusters formed during ESI spray processes and interferences from sample matrix. Repeatability and reproducibility studies showed that the LC/MS/MS method is a reliable and reproducible method which can provide a typical method precision of 1.0% while the LC/MS results are influenced by chemical interferences.

• Journal of Internet Computing and Services
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• v.5 no.6
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• pp.21-30
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• 2004

In Ad-Hoc network, the cluster structure enables effective use of multiple channels, reduces the number of control messages, and increase the scalability of network, Also, it is employed for reducing the number of broadcast messages in an Ad-Hoc network. With the consideration of these advantages, it is desirable that a cluster structure keeps a few clusters in the network, Generally, the cluster formation scheme based on connectivity yields fewer clusters than the other schemes. However, the connectivity based scheme may yield even more clusters than the other schemes according to the network topology. In this paper, a cluster formation scheme dividing the cluster formation into two phases is proposed. In the first phase, the lowest connectivity host in neighborhood initiates the cluster formation. Then, an adjustment procedure for affiliating a lot of the lowest connectivity hosts is employed. In the second phase, the hosts which were not affiliated to the first phase clusters are grouped into one or more clusters through criterions of connectivity and host ID. As a result, the proposed scheme yields a fewer clusters compared with existing other schemes in fully distributed method. The simulation results proves that our scheme is better than LIDCP(3) and HCCP(3).

• Journal of the Korea Society of Computer and Information
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• v.24 no.10
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• pp.65-70
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• 2019

In recently, ship trajectory data consisting of ship position, speed, course, and so on can be obtained from the Automatic Identification System device with which all ships should be equipped. These data are gathered more than 2GB every day at a crowed sea port and used for analysis of ship traffic statistic and patterns. In this study, we propose a method to process ship trajectory data efficiently with distributed computing resources using MapReduce algorithm. In data preprocessing phase, ship dynamic and static data are integrated into target dataset and filtered out ship trajectory that is not of interest. In mapping phase, we convert ship's position to Geohash code, and assign Geohash and ship MMSI to key and value. In reducing phase, key-value pairs are sorted according to the same key value and counted the ship traffic number in a grid cell. To evaluate the proposed method, we implemented it and compared it with IALA waterway risk assessment program(IWRAP) in their performance. The data processing performance improve 1 to 4 times that of the existing ship trajectory analysis program.

• Korean Chemical Engineering Research
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• v.48 no.3
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• pp.359-364
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• 2010

Three kinds of functional regions such as continuous slurry(${\varepsilon}_f$), bubble(${\varepsilon}_b$) and wake(${\varepsilon}_w$) regions were identified, and the individual phase holdups of each functional region were determined in a three-phase slurry bubble column of 0.152 m ID. The holdups of bubble and wake were measured by adopting the electrical resistivity probe method. Effects of gas velocity and solid concentration in the slurry phase on the individual holdups of functional regions in the column were discussed. The holdup of continuous slurry phase decreased but that of bubble or wake increased, with an increase in the gas velocity in the column. The increase of solid content in the slurry phase could lead to the increase in the holdup of continuous slurry phase but decrease in the bubble or wake holdup. The portion of wake holdup was in the range of 15~40% of the bubble holdup, which decreased with increasing gas velocity or solid content in the slurry phase. The individual holdups of three functional regions were well correlated with operating variables within this experimental conditions.

• Journal of the Korean Geophysical Society
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• v.5 no.4
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• pp.283-292
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• 2002

The Korean peninsula is located at the edge of the East Asian active margin. The seismic activity in the Korean Peninsula is relatively low compared with the neighboring countries China and Japan. According to the available Seismic information, the Korean Peninsula is not totally safe from the Earthquake disaster. Moreover, the area is surrounded by varies tectonic forces which is resulted from the relative movements of the surrounding tectonic plates "Pacific, Philippine Sea, Eurasian and South China". Nowadays South Korea has 65 GPS stations belong to 5 governmental organizations "each organization figure out its own GPS stations for different requirements" In order to minimize the seismic hazard in the Korean Peninsula a program for monitoring the recent crustal movement has been designed considering the uses of the available GPS station "some selected stations from the previously mentioned stations" and the tectonic settings in and around the Korean Peninsula. This program is composed of two main parts, the first part to monitor the crustal deformation around the Korean Peninsula with the collaboration of the surrounding countries "China and Japan" this part is composed of two phases "East Sea Phase and Yellow Sea Phase". These phases will be helpful in determining the deformation parameters in the East Sea and the Yellow Sea respectively While the Second part of this program, is designed to determine the deformation parameters id and around the main faults in the Korean Peninsula and the relative movement between the Korean Peninsula and the Cheju Island. Through out this study the needs of crustal movement center rose up to collect the data from the previously mentioned stations and Organizations in order to use such reliable data in different geodynamical application.