• 분석과학
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• 제25권1호
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• pp.25-32
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• 2012

동위원소희석 액체크로마토그래피-탄뎀질량분석법을 이용하여 차음료 중의 소르빈산을 정밀하게 측정하는 방법을 개발하였다. 소르빈산의 동위원소, $^{13}C_2$-소르빈산은 주문합성하여 구입하였다. 질량 분석기는 음이온 모드로 선택반응분석법 (Selected Reaction Monitoring mode)에서 수행하여, $[M-H]^-$ 이온이 $[M-CO_2H]^-$ 조각이온으로 가는 m/z 111 ${\rightarrow}$ 67와 $^{13}C_2$-소르빈산 m/z 113 ${\rightarrow}$ 68 채널을 선택하여 분석하였다. 분리는 C18 컬럼으로 수행되었으며, 암모니움 아세테이트용액(pH 4.5) 55%와 메탄올 45%로 이동상을 구성하였다. 반복성과 재현성을 포함한 방법의 유효성 검토를 위하여 본 연구원에서 차음료에 소르빈산을 강화하여 균질성있는 기준물질을 제조하였다. 반복성과 재현성 결과는 동위원소희석 액체크로마토그래피-탄뎀질량분석법에 의한 소르빈산의 분석이 신뢰할만 하며 재현성 있는 방법임을 보여주었고, 기준물질들의 소르빈산의 농도는 3.8%의 표준편차를 보여주었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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• pp.187-187
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• 2012

In this report, the plasmonic nanopores of less than 5 nm diameter were fabricated on the apex of the pyramidal cavity array. The metallic pyramidal pit cavity can also utilized as the plasmonic bioreactor, and the fabricated Au or Al metallic nanopore can provide the controllable translocation speed down using the plasmonic optical force. Initially, the SiO2 nanopore on the pyramidal pit cavity were fabricated using conventional microfabrication techniques. Then, the metallic thin film was sputter-deposited, followed by surface modification of the nanometer thick membrane using FESEM, TEM and EPMA. The huge electron intensity of FESEM with ~microsecond scan speed can provide the rapid solid phase surface transformation. However, the moderate electron beam intensity from the normal TEM without high speed scanning can only provide the liquid phase surface modification. After metal deposition, the 100 nm diameter aperture using FIB beam drilling was obtained in order to obtain the uniform nano-aperture. Then, the nanometer size aperture was reduced down to ~50 nm using electron beam surface modification using high speed scanning FESEM. The followed EPMA electron beam exposure without high speed scanning presents the reduction of the nanosize aperture down to 10 nm. During these processes, the widening or the shrinking of the nanometer pore was observed depending upon the electron beam intensity. Finally, using 200 keV TEM, the diameter of the nanopore was successively down from 10 nm down to 1.5 nm.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 하계학술대회 논문집 A
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• pp.121-123
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• 1996

Capacitor-run motor has a capacitor id series with the auxiliary winding for normal running connections. After the shape of stator and rotor are determined, the motor is designed with variables such as winding distributions or capacitance except punching variables. In this paper, the winding distribution and the turn ratio was taken as design variables because the winding distribution affects the torque and efficiency. And capacitance was selected as an additional variable. Simulation results show the validity of proposed method.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1977-1983
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• 2006

Kaempferol was isolated and identified from the methanol extract of the flowers of Impatiens balsamina. Kaempferol showed inhibitory activity against mushroom tyrosinase with an $ID_{50}$ of 0.042 mM. Inhibition kinetics, as determined using a Lineweaver-Burk plot, showed kaempferol to be a competitive inhibitor of mushroom tyrosinase with a $K_i$ value of 0.011 mM. The lag phase of tyrosine hydroxylation catalyzed by mushroom tyrosinase clearly increased on increasing the concentration of kaempferol. In addition to its tyrosinase inhibiting activity, kaempferol strongly inhibited melanin production by Streptomyces bikiniensis, in a dose-dependent manner, without inhibiting cell growth. For comparative purposes, the tyrosinase inhibitory activity of kaempferol was also assayed versus quercetin, a positive standard.

• Journal of Power Electronics
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• 제14권6호
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• pp.1272-1280
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• 2014

This paper presents solutions for fault detection and diagnosis of two-level, three phase voltage-source inverter (VSI) topologies with IGBT devices. The proposed solutions combine redundant standby VSI structures and contactors (or relays) to improve the fault-tolerant capabilities of power electronics in applications with safety requirements. The suitable combination of these elements gives the inverter the ability to maintain energy processing in the occurrence of several failure modes, including short-circuit in IGBT devices, thus extending its reliability and availability. A survey of previously developed fault-tolerant VSI structures and several aspects of failure modes, detection and isolation mechanisms within VSI is first discussed. Hardware solutions for the protection of power semiconductors with fault detection and diagnosis mechanisms are then proposed to provide conditions to isolate and replace damaged power devices (or branches) in real time. Experimental results from a prototype are included to validate the proposed solutions.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 B
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• pp.1044-1048
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• 2001

This paper proposes a nonlinear impedance circuit composed by diodes and inductors or capacitors. This circuit needs no control circuits and switches, and the impedance value is changed by the polarity of current or voltage. This paper presents one of these applications to improve the input current of capacitor input diode rectifiers. The rectifier using the nonlinear impedance circuit id constructed with four diodes and four capacitors in addition to the conventional rectifiers, that is, it has eight diodes and five capacitors, including a DC link capacitor. It makes harmonic components of the input current reduce and the power factor improve. A circuit design method is shown by experimentation and confirmed simulation. It explained that compared conventional pulse-width modulated (PWM)inverter with half pulse-width modulated (HPWM) inverter proposed HPWM inverter eliminated dead-time by lowering switching loss and holding over-shooting.

• 한국식품위생안전성학회지
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• 제9권1호
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• pp.37-42
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• 1994

Four sulfoanmides ; sulfamerazine ; sulfamethazine, sulfathiazole and sulfadimethoxine from muscle, kindney, liver and heart tissues of pork and chicken by LC. Residual sulfonamides were extracted with dichloromethane and determined on a Sperisorb ODS-1 column(250mm$\times$4.6mm id) with acetonitrile/water/acetic acid (30/70/0.3 v/v) as a mobile phase at 260nm. Recoveries from 4 tissues of pork and chicken samples fortified with 50 and 100 ppb were 71.2~87.2% and 73.7~89.6%, respectively. The detection limit was 0.03 $\mu\textrm{g}$/g in each drug. Sulfamethazine in 5 samples of pork. And sulfadimethoxine in 5 samples and sulfamethazine in 3 samples were also detected from 41 samples of chicken. The order of residue levels of sulfonamides in tissues was kidney>liver>heart>muscle, respectively. The residue levels of sulfonamides from kidney and liver were 0.03~0.15 $\mu\textrm{g}$/g in porks and 0.03~0.10 $\mu\textrm{g}$/g in chickens.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.358-360
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• 2007

In this paper, an auto-diagnosis method of the stator winding fault for small induction motor is suggested. 3-phase stator currents are sampled, filtered, and transformed with Park's vector transformation. After then Park's vector patterns are obtained. To detect the stator winding fault automatically, a distortion ratio (id/iq) is newly defined and compared with the one of healthy motor, and the threshold levels are suggested. The 2-turn, 4-turn, 8-turn winding fault are tested with no load, 25%, 50%, 75%, and 100% rated load. The distortion ratio of the Park's vector patterns are increased as the increase of the faulted turns, but are same as the increase of the load.

• 한국식품영양과학회지
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• 제26권6호
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• pp.1258-1267
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• 1997

Corbon dioxide id effective for extending the shelf-life of perishable foods by retarding microbial growth. The overall effect of carbon dioxide is to increase both the lag phase and generation time of microorganisms. However, the role of carbon dioxide in affecting the growth and metabolism of any given microorganisms is not clear yet, although its inhibitory effect is generally found at moderate to high concentrations. Systematic studies of the effects of carbon dioxide on microorganisms are therefore warranted. It is also necessary to understand the role of carbon dioxide in the preservation of foods as well as the control by carbon dioxide of fermentations of biotechnological importance. In this review, the antimicrobial effect of carbon dioxide on microorganisms is investigated in terms of its gas and solution properties, inhibition of microbial growth and specific metabolic processes, perturbation of membrane structure.

• 한국산학기술학회논문지
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• 제10권10호
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• pp.2750-2759
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• 2009

본 논문은 2008년 Bindu 등이 제안한 프로토콜의 취약점을 분석하고, 이를 해결할 수 있는 향상된 프로토콜을 제안한다. 제안한 프로토콜은 안전성면에서 타임 스탬프를 사용하지 않고 랜덤 수를 사용하여 제한된 재전송 공격과 서비스 거부 공격을 방지할 수 있다. 이와 더불어 사용자의 ID 정보를 AES로 암호화하여 전송함으로써 사용자의 익명성을 제공하였다. 또한, 멱승 연산을 제거하고 사용자가 자유롭게 패스워드를 변경할 수 있는 패스워드 변경 단계를 추가하여 프로토콜의 효율성을 높였다. 논문에서는 제안한 프로토콜을 STM 스마트 카드에 직접 구현하고 인증 서버를 설치하여 그 동작이 정확하고 효율적임을 검증하였다.