• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.668-673
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• 2013

A recombinant E. coli co-expressing ALA synthase (hemA), NADP-dependent malic enzyme (maeB), and dicarboxylic acid transporter (dctA) was reported to synthesize porphyrin derivatives including iron-containing heme. To enhance the synthesis of bacterial heme, five genes of the porphyrin biosynthetic pathway [pantothenate kinase (coaA), ALA dehydratase (hemB), 1-hydroxymethylbilane synthase (hemC), uroporphyrinogen III synthase (hemD), and uroporphyrinogen III decarboxylase (hemE)] were amplified in the recombinant E. coli co-expressing hemA-maeB-dctA. Pantothenate kinase expression enabled the recombinant E. coli to accumulate intracellular CoA. Intracellular ALA was the most enhanced by uroporphyrinogen III synthase expression, porphobilinogen was the most enhanced by ALA dehydratase expression, uroporphyrin and coproporphyrin were the most enhanced by 1-hydroxymethylbilane synthase expression. The strain co-expressing coaA, hemA, maeB, and dctA produced heme of $0.49{\mu}mol/g$-DCW, which was twice as much from the strain without coaA expression. Further pathway gene amplifications for the porphyrin derivatives are discussed based on the results.

• 한국의류학회지
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• 제5권2호
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• pp.49-53
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• 1981

This study was carried out to investigate the drapability of polyester double jersey skirt Drapability is an important aesthetic properties of fabric on clothing construction, In this thesis weight, shearing, bending and non-isotropic characteristics of fabric were regarded as important factors of drapability, Especially for drapability of skirt, I investigated hem effect on various length of hem and skirt, The results were as follows, 1. The less the weight of fabric was, the greater drapability appeared. On fabrics, large pliability and modulus of shear have good drapability, 2. On clothing cutting, non-isotropic property affected on drapability of clothes remark-ably. Drapability order of clothes was greatest in bias direction, next in wale, course direction, 3. The shorter the skirt length and closer at hem line were the larger the hem effect influence upon the drapability of skirt was.

• 한국의상디자인학회지
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• 제15권1호
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• pp.21-30
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• 2013

The purpose of this study is to recognize the differences of visual evaluation by variations in silhouette of the wide pants. The stimuli are 9 samples: One control group, 3 variations of the width of hem line and 3 variations of the silhouette. The data has been obtained from 57 fashion college students. The data has been analyzed by Factor Analysis, Anova, Scheffe's Test. The results of the study are as follows: The visual evaluation by silhouette of wide pants are composed of 5 factors(i.e., physical characteristics, complexity, attractiveness, originality, and stiffness and softness). Among these factors, the physical characteristic is evaluated to be the most important factor. The straight pants were the most attractive and modern when the width of the hem line was 66cm (1st stage), while the pants were uncomfortable and unwearable when the width of the hem line was 98cm (3rd stage). The bell-bottom pants were evaluated to be the most attractive with the average width of the hem line. When the width of the hem line of the flare pants were too wide, it was uncomfortable and unwearable but had its unique originality. When the width of the hem line was narrow, the visual image changed as the pants' silhouette varied. However, when the width of the hem line was wide, the visual image did not change by silhouette.

• 한국환경농학회지
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• 제21권1호
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• pp.45-49
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• 2002

하수슬러지 중에 존재하는 HEM에 대해 분석하고, 이를 토양에 사용할 때 발생하는 연간 오염부하량에 대해 추정하였다. 전국의 하수처리장에서부터 수집된 하수슬러지 시료 84점에 대해 분석한 HEM의 평균함량은 27.7$\pm$26.5 g/kg이었고, 최소 1.05에서 최대 194 g/kg을 나타내어 분석시료간에 큰 편차를 보였다. 하수처리장이 위치한 도시의 규모에 따라 5단계로 나누었을 때 HEM의 함량은 광역시, 대도시, 중도시, 소도시, 농촌지역에 각각 22.7$\pm$6.7, 33.3$\pm$25.8, 22.0$\pm$8.7, 31.0$\pm$38.8, 27.7$\pm$25.1 g/kg을 나타내었다. 미국 EPA의 하수슬러지 토양시용량 기준을 적용하여 농경지, 산림, 공공용지, 개량용지로 분류하고 각각 7,000, 26,000, 18,000, 74,000 kg/ha (건물량 기준)를 하수슬러지 최대 사용량으로 가정하여 HEM의 연간 오염부하량을 계산하였다. 건물량 기준으로 시용량이 7,000 kg/ha인 농경지의 경우 최대 1,032 kg/ha의 HEM 부하량을 나타내었고, 개량용지 시용기준인 74,000 kg/ha를 적용한 경우 HEM의 연간 오염부하량은 10,908 kg/ha으로 추정되었다. 그 외 산림기준을 적용할 때는 최대 3,832 kg/ha, 공공용지 기준을 적용할 때는 최대 2,653 kg/ha의 연간 오염부하량이 추정되었다.

• 복식문화연구
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• 제17권3호
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• pp.499-511
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• 2009

As the demand of the consumer for high-sense clothing, relation with materials is becoming important even in clothing construction. Especially, the cutting angle of materials is becoming an crucial element in the formation of silhouette, and drape of Hem line, of skirts. Accordingly, in this study, Hem line shapes between real clothing and 3D virtual clothing of "i-Designer" were analyzed by manufacturing semi-flare skirts of polyester 100% according to a cutting angle, and the results are as follows. As a result of comparison of silhouette between the real clothing and the virtual clothing, the real and the virtual have a similar feeling regardless of a cutting angle. In case of drape shapes, both the front and the lateral side were represented in almost similar shapes. The back side, however, the real and the virtual showed a great difference in case of grain direction. And in the whole silhouette, the real and the virtual were represented similarly. It could be known that with regard to Hem-line drape shapes between the real clothing and the virtual according to a cutting angle, the real clothing is represented in a location farther from the body than the virtual clothing and, the location or number of node was similarly showed in the real and the virtual.

• 복식
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• 제33권
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• pp.217-228
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• 1997

The purpose of this study was to investigate influence of the construction method of the circular skirt on drapability and shape of static silhouette and length variation. In made 24 types of skirts giving the variations (six kinds of peach skin-like finished fabrics two types of cutting method two types of machine stitch method of hem), The results were as follows: 1. Analysis of drapability In the cutting method thre was similarity between warp direction and true bias direc-tion. In the machine stitch method of hem there was similarity between blind stitch and blind stitch machine. According to the fabrics analysis of drapability was excellent in the order N/P 80/20(fabric 1)>P 100(fabric 5)>P 100(fabric 6)>P 100 (fabric 2)>P 100 (fabric 4)>N/C 50/50(fabric 3). 2. Analysis of the characteristics values of static silhouette shape In the cutting method shape of static sil-houette became wide in the warp direction. In the machine stitch method of hem shape of static silhouette became wide in the blind stitch. According to the fabrics shape of static silhouette became most wide in the N/C 50/50(fabric 3) and shape of static sil-houette become most narrow in the P 100(fabric 2,5) 3. Analysis of the length variation In the cutting method the true bias direc-tion was longer than the warp direction. In the machine stitch method of hem there was simi-machine stitch method of hem there was simi-larity between blind stitch and blind stitch ma-chine. In fabrics the P 100(fabric 6) showed the longest the N/C 50/50(fabric 3) showed most slight. Interaction between the cutting and messure-ment part of skirt lengh. In the warp diretion parts that showed longest length variation were C. G, K, O in the true bias diretion parts that showed most slight length variation were A, E, M, I.

• 대한소아치과학회지
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• 제33권2호
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• pp.304-310
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• 2006

본 연구는 치주질환 주요 병인균주 중의 하나인 P. intermedia를 대상으로 하여, 이 균주에서의 hemin 결합 단백질 유전자를 분리하고 염기서열을 결정하기 위하여 수행되었다. 본 연구에서는 약 5,000개의 recombinant colony들을 스크리닝하여 hemin 결합 단백질 유전자를 포함하고 있는 것으로 여겨지는 1개의 클론(pHem1)을 확인하였다. Restriction enzyme mapping 결과 pHem1의 insert DNA의 크기는 약 2.5kb이었으며 P. intermedia chromosomal DNA 내에는 hemin 결합 단백질 유전자가 single copy로 존재하였고, transcript의 크기는 약 1.8kb이었다. 분리한 pHem1 유전자는 1개의 ORF를 가지고 있으며, ORF의 크기는 2,550bp로 약 850개의 아미노산 폴리펩타이드로 구성되어 있다. 또한, pHem1 유전자는 이미 밟혀진 다른 유전자들과 상동성을 보이지 않았다. 본 연구는 P. intermedia에서의 porphyrin생리 및 hemin 획득기전을 분자생물학적으로 규명하는데 있어 중요한 의의가 있으리라 사료된다. 향후 pHem1 유전자의 특성 분석 등이 수행되어야 할 것으로 여겨진다.

• 한국전자파학회논문지
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• 제14권6호
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• pp.655-662
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• 2003

유전체 봉이 삽입된 원통형 공진기의 정밀한 필드 해석이 비소멸 모드와 컨투어 그래프 방법에 의해 제안되었다. 공진주파수들과 저차 모드들이 유전체 봉이 삽입된 원통형 공진기에 대해 계산되었다. 공진기의 설계를 위해 모드 차트가 제안되었다. 비소멸 모드 해석을 사용하여 하이브리드 모드들(TE, TM, HEM 모드)이 상세하게 해석되었고, 모드 차트를 완성하였다. 이론적 타당성은 실험에 의해 확인하였다. 이론과 실험 결과는 전반적으로 만족하였다. TE 모드, TM 모드, HEM 모드의 실험값과 이론값의 평균 오차는 각각 0.20 %, 0.14 %, 0.28 %이다.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.880-886
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• 2015

Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. coli-synthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosynthetic pathways, an engineered E. coli artificially overexpressing ALA synthase (hemA from Rhodobacter sphaeroides) and pantothenate kinase (coaA gene from self geneome) was constructed as a bacterial heme-producing strain, and both the transcription levels of pathway genes and the intermediates concentrations were determined from batch and continuous cultures. Transcription levels of the pathway genes were not significantly changed among the tested conditions. Intracellular intermediate concentrations indicated that aminolevulinic acid (ALA) and coenzyme A (CoA) were enhanced by the hemA-coaA co-expression. Intracellular coproporphyrinogen I and protoporphyrin IX accumulation suggested that the bottleneck steps in the heme biosynthetic pathway could be the spontaneous conversion of HMB to coproporphyrinogen I and the limited conversion of protoporphyrin IX to heme, respectively. A strategy to increase the conversion of ALA to heme is discussed based on the results.

• 약학회지
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• 제46권1호
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• pp.24-29
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• 2002

Enoyl-Acyl Carrier Protein Reductase (Fabl) of bacteria is hem as an important target for new antibacterial drugs and plays a determinant role in completing cycles of elongation in type-H fatty acid synthase system. In this study, a fabI gene from Staphylococcus aureus 6538p cloned in pET-l4b vector and FabI protein was over-produced in Escherichaia coli BL2l (DE3). $NH_2$-terminal His-tagged FabI protein was purified by nickel-nitrilotriacetic acid (Ni-NTA) metalaffinity chromatography Purified 6xHis-tagged FabI showed a catalytic activity on tram - 2 - octenoyl - N -acethlcysteamine by utilizing NADPH as a cofactor. For the discovery of new FabI inhibitors from chemical libraries, a target-oriented screening system using a 96-well plate was developed. About 10,000 chemical libraries from Korea Chemical Bank wore tested in this screening system, and 26 chemicals (0.25%) among them showed an inhibitory activity against FabI enzyme. This result showed that a new screening system can be used for the discovery of new FabI inhibitors.