• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.668-673
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• 2013

A recombinant E. coli co-expressing ALA synthase (hemA), NADP-dependent malic enzyme (maeB), and dicarboxylic acid transporter (dctA) was reported to synthesize porphyrin derivatives including iron-containing heme. To enhance the synthesis of bacterial heme, five genes of the porphyrin biosynthetic pathway [pantothenate kinase (coaA), ALA dehydratase (hemB), 1-hydroxymethylbilane synthase (hemC), uroporphyrinogen III synthase (hemD), and uroporphyrinogen III decarboxylase (hemE)] were amplified in the recombinant E. coli co-expressing hemA-maeB-dctA. Pantothenate kinase expression enabled the recombinant E. coli to accumulate intracellular CoA. Intracellular ALA was the most enhanced by uroporphyrinogen III synthase expression, porphobilinogen was the most enhanced by ALA dehydratase expression, uroporphyrin and coproporphyrin were the most enhanced by 1-hydroxymethylbilane synthase expression. The strain co-expressing coaA, hemA, maeB, and dctA produced heme of $0.49{\mu}mol/g$-DCW, which was twice as much from the strain without coaA expression. Further pathway gene amplifications for the porphyrin derivatives are discussed based on the results.

• Journal of the Korean Society of Clothing and Textiles
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• v.5 no.2
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• pp.49-53
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• 1981

This study was carried out to investigate the drapability of polyester double jersey skirt Drapability is an important aesthetic properties of fabric on clothing construction, In this thesis weight, shearing, bending and non-isotropic characteristics of fabric were regarded as important factors of drapability, Especially for drapability of skirt, I investigated hem effect on various length of hem and skirt, The results were as follows, 1. The less the weight of fabric was, the greater drapability appeared. On fabrics, large pliability and modulus of shear have good drapability, 2. On clothing cutting, non-isotropic property affected on drapability of clothes remark-ably. Drapability order of clothes was greatest in bias direction, next in wale, course direction, 3. The shorter the skirt length and closer at hem line were the larger the hem effect influence upon the drapability of skirt was.

• Journal of the Korea Fashion and Costume Design Association
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• v.15 no.1
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• pp.21-30
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• 2013

The purpose of this study is to recognize the differences of visual evaluation by variations in silhouette of the wide pants. The stimuli are 9 samples: One control group, 3 variations of the width of hem line and 3 variations of the silhouette. The data has been obtained from 57 fashion college students. The data has been analyzed by Factor Analysis, Anova, Scheffe's Test. The results of the study are as follows: The visual evaluation by silhouette of wide pants are composed of 5 factors(i.e., physical characteristics, complexity, attractiveness, originality, and stiffness and softness). Among these factors, the physical characteristic is evaluated to be the most important factor. The straight pants were the most attractive and modern when the width of the hem line was 66cm (1st stage), while the pants were uncomfortable and unwearable when the width of the hem line was 98cm (3rd stage). The bell-bottom pants were evaluated to be the most attractive with the average width of the hem line. When the width of the hem line of the flare pants were too wide, it was uncomfortable and unwearable but had its unique originality. When the width of the hem line was narrow, the visual image changed as the pants' silhouette varied. However, when the width of the hem line was wide, the visual image did not change by silhouette.

• Korean Journal of Environmental Agriculture
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• v.21 no.1
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• pp.45-49
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• 2002

To estimate annual pollutant loading rate of n-hexane extractable material (HEM) to soil 84 sewage sludge samples were analyzed. The average content of HEM was 27.7$\pm$26.5 g/kg, and ranged from 1.05 to maximum 194 g/kg. According to the number of population of the city the areas were classified as five regions, i.e. big, large, middle, small, and rural area in which the waste water treated plants were grown. The contents of HEM were 22.7$\pm$16.7, 33.3$\pm$25.8, 22.0$\pm$8.7, 31.0$\pm$38.8, 27.7$\pm$25.1 g/kg, in big, large, middle, small, and rural area, respectively. The applicated areas with sewage sludge to land also were divided as 4 sites by US EPA criteria such as agricultural, forest, public contact sites, and reclamation site. The quantitation of annual pollutant loading rate was estimated using a function (99 percentile concentration = $\bar{X}$ + 3$\sigma$) when the application amounts were in dosages of 7,000, 26,000, 18,000, 74,000 kg/ha in 5 regions, respectively. The annual pollutant loading rate of HEM to soil by sewage sludge application was maximum 1,032, 3,832, 2,653, and 10,908 kg/ha in agricultural, forest, public contact sites, and reclamation site, respectively.

• The Research Journal of the Costume Culture
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• v.17 no.3
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• pp.499-511
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• 2009

As the demand of the consumer for high-sense clothing, relation with materials is becoming important even in clothing construction. Especially, the cutting angle of materials is becoming an crucial element in the formation of silhouette, and drape of Hem line, of skirts. Accordingly, in this study, Hem line shapes between real clothing and 3D virtual clothing of "i-Designer" were analyzed by manufacturing semi-flare skirts of polyester 100% according to a cutting angle, and the results are as follows. As a result of comparison of silhouette between the real clothing and the virtual clothing, the real and the virtual have a similar feeling regardless of a cutting angle. In case of drape shapes, both the front and the lateral side were represented in almost similar shapes. The back side, however, the real and the virtual showed a great difference in case of grain direction. And in the whole silhouette, the real and the virtual were represented similarly. It could be known that with regard to Hem-line drape shapes between the real clothing and the virtual according to a cutting angle, the real clothing is represented in a location farther from the body than the virtual clothing and, the location or number of node was similarly showed in the real and the virtual.

• Journal of the Korean Society of Costume
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• v.33
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• pp.217-228
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• 1997

The purpose of this study was to investigate influence of the construction method of the circular skirt on drapability and shape of static silhouette and length variation. In made 24 types of skirts giving the variations (six kinds of peach skin-like finished fabrics two types of cutting method two types of machine stitch method of hem), The results were as follows: 1. Analysis of drapability In the cutting method thre was similarity between warp direction and true bias direc-tion. In the machine stitch method of hem there was similarity between blind stitch and blind stitch machine. According to the fabrics analysis of drapability was excellent in the order N/P 80/20(fabric 1)>P 100(fabric 5)>P 100(fabric 6)>P 100 (fabric 2)>P 100 (fabric 4)>N/C 50/50(fabric 3). 2. Analysis of the characteristics values of static silhouette shape In the cutting method shape of static sil-houette became wide in the warp direction. In the machine stitch method of hem shape of static silhouette became wide in the blind stitch. According to the fabrics shape of static silhouette became most wide in the N/C 50/50(fabric 3) and shape of static sil-houette become most narrow in the P 100(fabric 2,5) 3. Analysis of the length variation In the cutting method the true bias direc-tion was longer than the warp direction. In the machine stitch method of hem there was simi-machine stitch method of hem there was simi-larity between blind stitch and blind stitch ma-chine. In fabrics the P 100(fabric 6) showed the longest the N/C 50/50(fabric 3) showed most slight. Interaction between the cutting and messure-ment part of skirt lengh. In the warp diretion parts that showed longest length variation were C. G, K, O in the true bias diretion parts that showed most slight length variation were A, E, M, I.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.2
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• pp.304-310
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• 2006

Prevotella intermedia is one of the most frequently implicated pathogens in human periodontal disease and has a requirement for hemin for growth. This study has identified a hemin-binding P. intermedia protein by expression of a P. intermedia genomic library in Escherichia coli, a bacterium which does not require or transport exogenous hemin. The genomic library of P. intermedia was constructed into plasmid pUC18, transformed into Escherichia coli strain $DH5{\alpha}$, and screened for recombinant clones using heminbinding activity by plating onto hemin-containing agar. Approximately 5,000 recombinant E. coli colonies were screened onto LB-amp-hemin agar, single clone(pHem1) was exhibited a clearly pigmented phonotype. The 2.5kb insert DNA of pHem1 was determined by restriction enzyme mapping. Southern blot analysis of BamHI, BglII, EcoRI, HindIII and PstI-digested P. intermedia DNA indicated that single copy of the gene was present in the genome. Northern blot analysis revealed that the size of transcript was approximately 1.8 kb. The cloned gene contained a single ORF, consisting of approximately 850-residue amino acids. A BLAST search of the Institute for Genomic Research genes with similar nucleotide sequence revealed no significant similarity It needs further investigation to clarify the mechanisms of heme uptake in P. intermedia.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.6
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• pp.655-662
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• 2003

A rigorous field analysis by the non-decaying mode and the contour graph method is presented for dielectric-rod loaded cylindrical cavity resonators. Resonant frequencies and the lowest order modes for this resonator are calculated. The mode charts are presented to design the resonators. TE mode(transverse electric or H mode, having no Ez), TM mode(transverse magnetic or I mode, having no ㎐) and HEM mode(hybrid electromagnetic mode, having non-zero Ez and ㎐) are analyzed in detail using non-decaying mode method. The mode charts are completed. The validity of the theory is confirmed by experiments. The results were on the whole satisfactory. Experimental measurements show excellent agrement with the numerical results. The average error of TE, TM, and HEM mode is about 0.20 %, 0.14 %, and 0.28 %, respectively.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.880-886
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• 2015

Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. coli-synthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosynthetic pathways, an engineered E. coli artificially overexpressing ALA synthase (hemA from Rhodobacter sphaeroides) and pantothenate kinase (coaA gene from self geneome) was constructed as a bacterial heme-producing strain, and both the transcription levels of pathway genes and the intermediates concentrations were determined from batch and continuous cultures. Transcription levels of the pathway genes were not significantly changed among the tested conditions. Intracellular intermediate concentrations indicated that aminolevulinic acid (ALA) and coenzyme A (CoA) were enhanced by the hemA-coaA co-expression. Intracellular coproporphyrinogen I and protoporphyrin IX accumulation suggested that the bottleneck steps in the heme biosynthetic pathway could be the spontaneous conversion of HMB to coproporphyrinogen I and the limited conversion of protoporphyrin IX to heme, respectively. A strategy to increase the conversion of ALA to heme is discussed based on the results.

• YAKHAK HOEJI
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• v.46 no.1
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• pp.24-29
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• 2002

Enoyl-Acyl Carrier Protein Reductase (Fabl) of bacteria is hem as an important target for new antibacterial drugs and plays a determinant role in completing cycles of elongation in type-H fatty acid synthase system. In this study, a fabI gene from Staphylococcus aureus 6538p cloned in pET-l4b vector and FabI protein was over-produced in Escherichaia coli BL2l (DE3). $NH_2$-terminal His-tagged FabI protein was purified by nickel-nitrilotriacetic acid (Ni-NTA) metalaffinity chromatography Purified 6xHis-tagged FabI showed a catalytic activity on tram - 2 - octenoyl - N -acethlcysteamine by utilizing NADPH as a cofactor. For the discovery of new FabI inhibitors from chemical libraries, a target-oriented screening system using a 96-well plate was developed. About 10,000 chemical libraries from Korea Chemical Bank wore tested in this screening system, and 26 chemicals (0.25%) among them showed an inhibitory activity against FabI enzyme. This result showed that a new screening system can be used for the discovery of new FabI inhibitors.