• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.286-291
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• 2002

Histone deacetylase I (HDAC1) works as one of the components in a nucleosome remodeling (NuRD) complex that consists of several proteins, including metastasis-associated protein 1 (MTA1). Since the protein-protein interaction of HDAC1 and MTA1 would appear to be important for both the integrity and functionality of the HDAC1 complex, the interruption of the HDAC1 and MTA1 interaction may be an efficient way to regulate the biological function of the HDAC1 complex. Based on this idea, a yeast two-hybrid system was constructed with HDAC1 and MTA1 expressing vectors in the DNA binding and activation domains, respectively. To verify the efficiency of the assay system, 3,500 microbial metabolite libraries were tested using the paper disc method, and KB0699 was found to inhibit the HDAC1 and MTA1 interaction without any toxicity to the wild-type yeast. Furthermore, KB0699 blocked the interaction of HDAC1 and MTA1 in an in vitro GST pull down assay and induced morphological changes in B16/BL6 melanoma cells, indicating the interruption of the HDAC1 complex function. Accordingly, these results demonstrated that the yeast assay strain developed in this study could be a valuable tool for the isolation of a HDAC1 complex disruptor.

• The Journal of the Korea Contents Association
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• v.10 no.6
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• pp.134-143
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• 2010

This paper proposes a framework for classifying metric-based software quality prediction models, especially case of software criticality, into four types. Models are classified along two vectors: input metric forms and the necessity of past project data. Each type has its own characteristics and its strength and weakness are compared with those of other types using newly defined criteria. Through this qualitative evaluation each organization can choose a proper model to suit its environment. My earlier studies of criticality prediction model implemented specific models in each type and evaluated their prediction performances. In this paper I analyze the experimental results and show that the characteristics of a model type is the another key of successful model selection.

• Journal of the Korean Operations Research and Management Science Society
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• v.12 no.2
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• pp.21-35
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• 1987

This paper presents a method for propagating fuzzy concepts through fuzzy IF-THEN-ELSE rules. A fuzzy IF-THEN-ELSE rule consists of a set of fuzzy condition and conclusion pairs. These pairs assumed to contain informations about a fuzzy mapping from fuzzy concepts of condition parts to the fuzzy concepts of conclusion parts. Conventionally, vectors are used to define fuzzy concepts and matrices are used to define a fuzzy mapping between fuzzy conditions and conclusions. This approach, however, does not satisfy the existing condition property, i.e., when a fuzzy input data exactly matches to a fuzzy condition, fuzzy output data should be mapped to a corresponding fuzzy conclusion. Alternatively, we propose a parameterized approach in which every fuzzy concept is described by a parameterized standard function, including fuzzy conditions and fuzzy conclusions. A fuzzy IF-THEN-ELSE rule takes the parameterized fuzzy concept as an input, and produces a standard function with new parameters as an output. New parameters are determined by a parameterwise interpolation. That is, each output parameters are determined by interpolating parameters of the same class contained in fuzzy conclusions. Obviously, the proposed scheme always satisfies the existing condition property.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.2
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• pp.281-287
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• 1999

The turbulent natural convection in the membrane type LNG carrier cofferdam with heating points has been studied by numerical method. As the numerical methods, we introduced the three turbulence model, a standard $k-{\varepsilon}$ model and two case of a low Reynolds number models. The parameters considered for this study ore number and capacity of heating points i.e., $1{\leq}Ns{\leq}12$ and $1.0{\times}10^5{\leq}Qs(W/m^3){\leq}1.0{\times}10^8$. The results of the isotherms and velocity vectors have been represented for various parameters. The temperature and velocity at upper position in the space ore shown to be higher than those at lower position. For obtaining the optimal temperatures, $20{\sim}30^{\circ}C$ in the cofferdam space, the heating capacities show $2.0{\times}10^7W/m^3$ at g-heating points and $1.0{\times}10^7W/m^3$ at 12-points. The mean temperature in the cofferdam space can be expressed as a function of number and capacity of heating points.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.55 no.5
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• pp.250-257
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• 2006

It is well known that Grain-oriented electrical steel sheets have two dimensional magnetic properties according to the direction of exciting field such as non-linear phase difference between magnetic flux density and magnetic field intensity vectors, different iron loss and permeability even when an alternating magnetic field is applied. The measurement and application of the two dimensional magnetic properties of the Grain-oriented electrical steel sheets, therefore, are very important for the design and precise performance analysis of electric machines made of them. As the direction of exciting field changes, in this paper, the two dimensional magnetic properties of a Grain-oriented electrical steel sheet, i.e., non-linear B-H curves, phase difference between B and H, and iron loss characteristics, are measured using SST(Single Sheet Tester) which has two axes excitation. The measured results are presented in two ways: using $(B,\theta_B)$ method and using hysteresis loops along rolling and transverse directions, respectively.

• Speech Sciences
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• v.6
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• pp.219-233
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• 1999

This paper presents a real-time implementation of a speaker independent speech recognition system based on a discrete hidden markov model(DHMM). This system is developed for a car navigation system to design on-chip VLSI system of speech recognition which is used by fixed point Oak DSP core of DSP GROUP LTD. We analyze recognition procedure with C language to implement fixed point real-time algorithms. Based on the analyses, we improve the algorithms which are possible to operate in real-time, and can verify the recognition result at the same time as speech ends, by processing all recognition routines within a frame. A car noise is the colored noise concentrated heavily on the low frequency segment under 400 Hz. For the noise robust processing, the high pass filtering and the liftering on the distance measure of feature vectors are applied to the recognition system. Recognition experiments on the twelve isolated command words were performed. The recognition rates of the baseline recognizer were 98.68% in a stopping situation and 80.7% in a running situation. Using the noise processing methods, the recognition rates were enhanced to 89.04% in a running situation.

• Journal of The Korean Astronomical Society
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• v.33 no.1
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• pp.47-55
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• 2000

Nonpotential characteristics of magnetic fields in AR 5747 are examined using Mees Solar Observatory magnetograms taken on Oct. 20, 1989 to Oct. 22, 1989. The active region showed such violent flaring activities during the observational span that strong X-ray flares took place including a 2B/X3 flare. The magnetogram data were obtained by the Haleakala Stokes Polarimeter which provides simultaneous Stokes profiles of the Fe I doublet 6301.5 and 6302.5. A nonlinear least square method was adopted to derive the magnetic field vectors from the observed Stokes profiles and a multi-step ambiguity solution method was employed to resolve the $180^{\circ}$ ambiguity. From the ambiguity-resolved vector magnetograms, we have derived a set of physical quantities characterizing the field configuration, which are magnetic flux, vertical current density, magnetic shear angle, angular shear, magnetic free energy density, a measure of magnetic field discontinuity MAD and linear force-free coefficient. Our results show that (1) magnetic nonpotentiality is concentrated near the inversion line in the flaring sites, (2) all the physical parameters decreased with time, which may imply that the active region was in a relaxation stage of its evolution, (3) 2-D MAD has similar patterns with other nonpotential parameters, demonstrating that it can be utilized as an useful parameter of flare producing active region, and (4) the linear force-free coefficient could be a evolutionary indicator with a merit as a global nonpotential parameter.

• Journal of IKEEE
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• v.6 no.1 s.10
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• pp.94-101
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• 2002

In this paper, we propose a motion estimation algorithm for the discontinuity blocks. The algorithm uses sub-SAD value (i.e. the sum of absolute difference for a quarter of a block) to identify the discontinuity region, and produces additional motion vectors for these sub-blocks if necessary. We show with experimental results that, in comparison with some conventional motion estimation algorithms, the proposed algorithm achieves quality enhancement for the sequences with discontinuity blocks, and also shows the same computational quantity as to normal algorithms for sequences with less discontinuity.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.351-355
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• 2005

In the storage roots of sweetpotato (Ipomoea batatas (L.) Lam. cv. Kokei 14), 10 to 20% of starch is essentially unbranched linear amylose and the other major component is branched amylopectin. Amylose is produced by the enzyme GBSSI (granule bound starch synthase I), whereas amylopectin is produced by a concerted action of soluble starch synthase and starch branching enzymes (SBEI and SBEII). We constructed double-stranded RNA (dsRNA) interference vectors of GBSSI and IbSBEII and introduced them into sweetpotato genome via Agrobacterium-mediated gene transformation. The endogenous GBSSI expression was inhibited by dsRNA of GBSSI in 73 % of transgenic plants giving rise to the storage tubers containing amylopectin but not amylose. On the other hand, all sweetpotato plants transformed with dsRNA of IbSBEII contained a larger amount of amylose than the non-transgenic control (up to 25% compared to 10% in the controls). The RNA interference (RNAi) is effectively inhibited the gene expression in thestarch metabolic pathway and modified the characteristics of starch in sweetpotato.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.323-330
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• 2019

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum-specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the transformation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.