• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.11-15
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• 2008

The expression of neuronal nitric oxide synthase (nNOS) is regulated by various spliced first exons (exon 1a-1i), sharing differentially common exon 2 in diverse human tissues. The highly complex structure and regulation of human nNOS gene gave limitations of information for the precise mechanism of nNOS regulation. In the present study, we report that the repeats of polymorphic dinucleotides $(GT)^nA(TG)^n$ repeats located in just upstream to the exon 1f in human nNOS gene play suppressive role in transcription, as shown in the characteristics of Z-DNA motif in other genes. In neuronal and trophoblast cells transfected transiently with luciferase construct without dinucleotide repeats at the 5'-flanking region of exon 1f in nNOS gene, the luciferase activity was increased markedly. However, the presence of the dinucleotide repeats dramatically suppressed the luciferase activity to the basal level, and which was dependent on the length of $(GT)^n$ and $(TG)^n$ repeats. More importantly, we found the polymorphisms in the length of dinucleotide repeats in human. Furthermore, we show for the first time here that there is a significant association of the lengths of polymorphic dinucleotide $(GT)^n$ and $(TG)^n$ repeats with the risk of schizophrenia.

• 한국의상디자인학회지
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• 제4권3호
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• pp.43-54
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• 2002

Some factors had influence upon the grotesque image formation expressed in fashion: for instance, thanatos, religions, fin de siecle (end of the century), the aesthetics of ugliness, subculture group's resistance and technology development, etc. Those factors have formed a grotesque while exchanging influence each other, and have following features: First, the thanatos, which is destructive and aggressive instinct of the inner world of human being, creates frightening object and motif to form the images of grotesque. Second, from religious point of view, the church made the Devil a tool for maintenance of power: They manipulated physical body and give a damage to it to sublimate it in holy existence, so that they could feel catharsis. Third, there was fin de siecle (end of the century) to let people have negative life attitude, such as uneasiness on following century, eschatology, skepticism and nihilism, etc. Fourth, the ugliness having unpleasantness and disharmony occupies governing position when our society becomes corrupted and uneasy, and the aesthetics of ugliness discloses the inconsistency of ideal and beautiful life in the grotesque images. Fifth, subculture groups, i.e., the lower classes, homosexual and the youth's group, etc, form the grotesque images by political and ideological resistance, complaints and specific identity, etc concerning governing culture keeping traditional ethics consciousness. Sixth, recent technology development has destroyed a boundary between human being and machinery, and bio-technology development has created transplant operation, plastic operation and other human body transformation operations, and genome research, etc has raised human being's identity.

• Interdisciplinary Bio Central
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• 제4권4호
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• pp.12.1-12.6
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• 2012

The advanced glycation endproducts receptor (AGE-R) is a signal transduction receptor for multiligand such as S100b and AGEs. S100b has been demonstrated to activate various cells with important links to atherosclerosis initiation and progression including endothelial cells, and smooth muscle cells via AGE-R, triggering activation of multiple signaling cascades through its cytoplasmic domain. Many studies have suggested AGE-R might even participate in the cardiovascular complications involved in the pathogenesis of type I diabetes. Recently, Small Ubiquitin-Related Modifier 1 (SURM-1 also known as SUMO-1) has been recognized as a protein that plays an important role in cellular post-translational modifications in a variety of cellular processes, such as transport, transcriptional, apoptosis and stability. Computer Database search with SUMOplot Analysis program identified the five potential SURMylation sites in human AGE-R: K43, K44, K123, and K273 reside within the extracellular domain of AGE-R, and lastly K374 resides with the cytosolic domain of AGE-R. The presence of the consensus yKXE motif in the AGE-R strongly suggests that AGE-R may be regulated by SURMylation process. To test this, we decided to determine if AGE-R is SURMylated in living vascular cell system. S100b-stimulated murine aortic vascular smooth muscle cells were used for western blot analysis with relevant antibodies. Taken together, bioinformatics database search and molecular biological approaches suggested AGE-R is SURMylated in living cardiovascular cell system. Whilst SURMylation and AGE-R undoubtedly plays an important role in the cardiovascular biology, it remains unclear as to the exact nature of this contribution under both physiological and pathological conditions.

• 복식
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• 제64권3호
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• pp.126-141
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• 2014

The purpose of this study is to analyze the specific mimesis types of common character costumes in MMORPGs, which have stories that are very similar in structure to ancient myths and fantasy novels. As the subject of the research I have selected "Diablo 3" created by the American video game developer, Blizzard Entertainment Images of 5 characters, for both genders - Barbarian, Witch Doctor, Monk, Demon hunter, Wizard - were compiled for a total of 10 images, and these images were compared to popular archetypes in mythology, religion and literature. analyzed them separately according to the mimesis type. For this study, Articles and books containing the Aesthetics, Mimesis and Digital culture were researched in order to study the costume features and the Mimesis types and compare them to the costumes in the MMORPG. Firstly, the character Barbarian is a successor of the barbarian character of the previous product. The outfit worn by the game figure is based on the common images expressed in the movie . The makers created an analogous-imitation mimesis by replicating the image in an exaggerated and grand form. Second, the character Witch Doctor derived its motif from the Voodoo cult, and was expressed in analogous-imitation mimesis, using the very shades and patterns used in real life. Third, the character Monk, blending the image of a priest in Eastern Europe and images of its oriental counterparts, was expressed in transposition-diversion mimesis. Fourth, the Demon Hunter took the source of its inspiration from the image of a Witch hunter in the medieval times. The game character has a sharper sleeker figure than its model, and uses more acute shapes and darker gloomier colors expressed in an analogous-imitation mimesis. Finally, the character Magician took a formless character and expressed it in transposition-diversion mimesis taking as its basis some fantasy novels.

• 한국동물위생학회지
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• 제42권4호
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• pp.245-250
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• 2019

Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the 'A' farm and 3~72 weeks old in the 'B' farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm 'A' and 'B' were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was 'GKQGRLIG' which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by entire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid sequence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.

• International Journal of Costume and Fashion
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• 제5권2호
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• pp.55-67
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• 2005

The purpose of this study is to document an unidentified velvet fabric which is located in the Museum. The investigation was conducted by analyzing color, weaving technique. pattern of the study object. To identify the origin of the object, many references about color trends, construction methods and patterns were accessed. Two comparative objects which were dating from 1600 to 1699 in the Museum of F.I.T. were selected since they are similar to the study object. The results of this study can be summarized as follows; (1) The type of this study object is 'cut and voided velvet'. It is obtained establishing the motif as areas of cut pile so as to form a pattern, while leaving other areas of the grounded weave without pile. Two flowers and two tulips with S curved stems are composed as one unit in the vertical direction. (2) The colors used in this study object. which are ivory, smoke blue, medium orange, and yellow. are part of a new trend color in the $17^{th}$ century. (3) The vertical undulating stripe patterns are also of significance in this fabric. They seemed to be contemporary with the serpentine line which was common since the $15^{th}$ century and the vertical stripes pattern which appeared from the $16^{th}$ century. (4) The stylized tulip patterns of this study object began to appear in the early $17^{th}$ century. The stems and leaves of this patterns are less sinuous than the floral motifs of the later $17^{th}$ century. Therefore, the study object is dated to early in $17^{th}$ century because of the color combination. stripes. stylized tulips, leaves. and stems are showing the characteristics of this period.

• 대한기계학회논문집A
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• 제24권7호
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• pp.1779-1786
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• 2000

Most of traditional design processes of mechanical parts are regarded as sequential and discrete, since different kinds of softwas should be introduced. In this paper, we develop an integrated fram ework for virtual design and performance testing of an automobile seat. The system is composed of four modules, i.e. CAD, static analysis, dynamic analysis, and draft drawing module. In the CAD module, PRO/ENGINEER 3D seat model is created using parameters to be modified with the result of static and dynamic analysis. In the static analysis, headrest tere used in each design stage make it difficult to feedback their results to upstream process. These discrete processes may result in time loss and cost rise. In recent years, life cycle of product is reduced. To have competence with others, new concept design processt is simulated using ANSYS. In the dynamic analysis module, FMVSS201 test is simulated using DADS. Overall data flow is controlled by Motif. The advantage of the system is that even a novice can perform and review the whole design process, without a good hand at professional design/analysis S/W in each stage. The system also provides a virtual design space, where engineers in different development stage can access common data of design models. The concept could be applied to other fields and it could reduce time and money required in design process.

• 한국의상디자인학회지
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• 제14권4호
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• pp.29-42
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• 2012

Historically, the pattern and technology of Copts' textiles, who were a minority in Egypt, have been studied a lot in the textile sector due to its unique characteristics. Unlike ones in other regions that appeared around the same time, the overall configuration ratio of the patterns looks exaggerated or distorted because they expressed it plainly by interpreting the world with ordinary people's eyes. Also, because it had used mixed linen and woolen yarns, harsh expression way and the use of various colors have been one of the features in Coptic textiles. Coptic textiles, which have been developed along with the historical development of continued domination from neighboring countries, have expressed the effects of the Roman Empire, Christ, Christianity, and Islam on the pattern of its fabric. This study analyzed its characteristics which make people smile by the way of expressing a simple and humorous representation of the textiles and categorized them as Humoristic Beauty - the aesthetic category of humorous feature. In this study, the Humoristic Beauty in Coptic textiles has been analyzed in terms of the following three smiles; the smiles coincidental with the flow of time, the smiles made by the shaping of distorted proportions and appearance, and the smiles like folk-paintings made by a rustic expression way. This study shows the possibility of the further studies on the textile patterns history from a different angle. I look forward to more detailed analysis in the follow-up studies in the future.

• 문화기술의 융합
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• 제4권1호
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• pp.231-236
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• 2018

꽃은 인간의 표현 욕구를 가장 아름답게 표현하는 자연의 소재 중 하나이다. 최근에는 이러한 꽃을 상품에 다양하게 응용해서 미적 가치를 높이고 생명력을 표현하는 예술적 소재로 사용하고 있다. 본 연구는 시각적으로 아름다운 형태를 가진 꽃과 우리 일상생활에서 사용하는 다양한 상품과의 융합을 통해 시각적으로 표현하고자 하는 조형성을 연구하였으며 다양한 상품에 응용된 꽃문양 모티브의 사례를 살펴보고 디자인의 요소와의 상관관계를 연구하였다. 이를 바탕으로 소비자에게 만족을 주는 감성디자인의 중요성과 새로운 디자인 표현기법을 연구하고 디자인 요소로 평가한 연구결과를 통해 꽃의 활용 가능성을 제시하고자 한다. 또한 상품의 일반적인 디자인 관점에서 벗어나 자연스러움과 독창적인 디자인 감각으로 상품의 심미적 가치를 추구하고 꽃의 이미지를 이용한 표현장식을 바탕으로 다양한 상품에 꽃 모티브를 접목시켜 디자인 감성을 표현하는 감성 상품디자인 개발의 가능성을 제시하고자 한다.

• 생명과학회지
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• 제22권3호
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• pp.293-297
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• 2012

분자 운동 단백질은 신경세포 내의 세포체에서 특정 목적지까지 소포를 이동시키는데 관여한다. 오징어의 거대 축삭은 간단한 제거조작으로 축삭을 분리 가능하기 때문에 신경세포내 물질이동기전 연구의 좋은 모텔로 활용 가능하다. 이전연구에서 오징어 거대축삭의 소포들은 미세소관을 따라 이동하는 키네신 항체에 의하여 운반됨이 확인되었다. 본 연구는 오징어 뇌에 존재하는 키네신들을 크로닝하고, 분리된 유전자의 분석을 행하기 위하여 키네신 운동 도메인에서 잘 보존된 아미노산 배열에 해당되는 영역에 DNA primer을 이용하여 새로운 6종류의 키네신을 분리하였다. 오징어의 키네신들과 생쥐의 키네신들의 motor 영역의 아미노산분석에서 보존된 영역이 존재하며, Maximum Parsimony (MP) 방법, Neighbor-Joining (NJ) 방법, Minimum Evolution (ME) 방법, 그리고 Maximum likelihood (ML) 방법을 기초로 한 계통분석에서 생쥐의 키네신과 높은 상동성을 나타내었으며, 또한 계통수에서도 높은 상관관계가 확인되었다.