• Title/Summary/Keyword: i-FP

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Electron Transport Carrier for the Free Radical Shethna Flavoprotein in Azotobacter vinelandii (Azotobacter vinelandii Shethna Flavoprotein 의 Free Radical 생성(生成)을 위(爲)한 전자전달물질(電子傳達物質)-)

  • Chun, Jae-Kun;Tollin, Gordon
    • Applied Biological Chemistry
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    • v.16 no.1
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    • pp.31-40
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    • 1973
  • Azotobacter vinelandii cell extracts and its variety of purified fractions with regard to their ability to form the redox state of the Shethna Flavoprotein (free radical form FPH.) were studied. A fluorescent flavoprotein (protein I) and a brown protein (protein II) were the most active proteins which were isolated in purified form. The free radical formation activity was substantially decreased during the purification and was completely lost upon storage in a week under nitrogen in a frozenstate. The presence of free flavin (FMN) with NADH enhanced the rate of free radical formation. The reaction of FMN and NADH was found to be catalysed by various cell fractions. A possible role of FMN as a substrate for free radical shethna flavoprotein was investigated. Slower reaction rate of $FMNH_2+Flavoprotein\;(FP){\to}FPH+FMN$ than $FMN+NADH{\to}FMNH_2$, accumulation of $FMNH_2$ ocurred which subsquently caused FPH.

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Optical, thermal and gamma ray attenuation characteristics of tungsten oxide modified: B2O3-SrCO3-TeO2-ZnO glass series

  • Hammam Abdurabu Thabit;Abd Khamim Ismail;M.I. Sayyed;S. Hashim;I. Abdullahi;Mohamed Elsafi;K. Keshavamurthy;G. Jagannath
    • Nuclear Engineering and Technology
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    • v.56 no.1
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    • pp.247-256
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    • 2024
  • The glass series modified by tungsten oxide was created using the compounds (75-x) B2O3- 10SrCO3- 8TeO2- 7ZnO - xWO3, where x = 0, 1, 5, 10, 22, 27, 34, and 40% mole percentage. A UV-visible spectrophotometer and thermogravimetric-differential thermal analysis (TG-DTA) methods were employed to characterize the specimen's optical and phase transition attributes, respectively. The mass-attenuation coefficient (AC) of all created glasses from BSTZW0 to BSTZ7 was estimated using Geant4 code from 0.05 to 3 MeV and compared to the XCOM software results, with a relative difference of less than 2% between the two results. The increase of WO3 percentage lead to an increase in the Linear-AC at each studied energy, and this is mainly due to the fact that the higher the percentage of WO3 in the glass increases its density which causes an increase in the Linear-AC, so an energy of 0.06 MeV, as an example, the values of the Linear-AC was 4.009, 4.509, 5.442, 6812, 8.564, 9.856, 10.999 and 11.628 cm-1 form BSTZW0 too BSTZW7, respectively. The Half-VL (value layer), Mean-FP (free path), Tenth-VL, and Radiation attenuation performance (RAP) were also calculated for the current BSTZW-glass samples and revealed that BSTZW7 had the best gamma ray attenuation performance at all discussed energies when compared to other studied glass samples.

A Kinematic Comparative Analysis of Yoko Ukemi(side breakfall) by Each Stage in Judo[ I ] (유도 단계별 측방낙법의 운동학적 변인 비교분석[ I ])

  • Kim, Eui-Hwan;Kim, Sung-Sup
    • Korean Journal of Applied Biomechanics
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    • v.14 no.3
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    • pp.203-218
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    • 2004
  • The purpose of this study was to analyze the comparisons of the kinematical variables when performing Yoko Ukemi(side breakfall) by three Stage in Judo. The subjects were four male judokas who were trainees Y. I. University Squad members and the Yoko Ukemi were filmed by two S-VHS 16mm video cameras(60fields/sec.). The selected times were subject to KWON 3D analysis program and kinematical analysis to compare variables of three Yoko Ukemi. Temporal variables(total time-required : TK, TR by each phase), the body part touched order on the mat and COG variables were computed through video analysis while performing right Yoko Ukemi by three stage. From the data analysis and discussion, the following conclusions were drawn : 1. Temporal variables : total time-required(TR) when performing Yoko Ukemi(side breakfall) by each stage, the first stage(full squat posture: FP : 1.11sec.) showed the shortest time, the next was 3rd(Shizenhontai, straight natural posture: NP : 1.41sec.), and 2nd(Jigohontai, straight defensive posture, DP : 1.42sec.), respectively- 2. TR when performing Yoko Ukemi(side breakfall) by each stage, and phase : the first phase(take of phase, average 0.68sec.) showed the longest time, next was the third phase(ukemi phase, 0.39sec.), and the second phase(air phase, 0.23sec.), respectively. 3. When performing yore Ukemi the body part touched order and TR on the mat : hip(0.94sec.) showed the shortest time, the next was elbow hand(0.97sec.), back(0.98sec.), and shoulder(1.04sec.) order. The hip part touched on the mat the first, but slap the mat in order to alleviate the shock try hand palm and forearm before receiving impact (difference 0.03sec,) 4. Vertical COG variables in each event by each stage : e1(ready position, average 78.33cm) moved the highest, the next was e2(jumping position, 70.14cm), e3(transition position, average 64.00cm), e4(landing position, average 35.99cm), and e5(ukemi position, average 18.32cm) order, gradual decrease respectively. And the difference of COG were showed in initial by each stage, because position fo Yoko Ukemi was difference by each stage in preparation position, but in accordance with executing of Ukemi phase that difference of COG was by decreasing, almost equal displacement in e4(landing) and e5(Ukemi)position finally.

Genetic Variations of Outer Membrane Protein Genes of Vibrio harveyi Isolated in Korea and Immunogenicity of OmpW in Olive Flounder, Paralichthys olivaceus (한국에서 분리된 Vibrio harveyi 외막단백질의 유전적 차이와 넙치(Paralichthys olivaceus)에 대한 OmpW의 면역원성 분석)

  • KIM, Myoung-Sug;JIN, Ji-Woong;JUNG, Sung-Hee;SEO, Jung-Soo;HONG, Suhee
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.5
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    • pp.1508-1521
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    • 2015
  • Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form ${\beta}-barrel$ with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of $4.2{\pm}0.7$ and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.

Photosynthetic Responses to Dehydration in Green Pepper(Capsicum annuum L.)Leaves

  • Lee, Hae-Yeon;Jun, Sung-Soo;Hong, Young-Nam
    • Journal of Photoscience
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    • v.5 no.4
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    • pp.169-174
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    • 1998
  • Photosynthetic responses to dehydration were examined by the simulataneous measurement of O2 evolution and chlorophyll (Chl) fluorescence in green pepper leaves. Dehydration was induced by immersing the plant roots directly in the Hoagland solution containing varying concentration (2-30%) of polyethylene glycol(PEG-6000) . Water potential of the leaf was decreased time-and concentation -dependently by PEG-treatment. The decrease in water potential of leaf was correlated with the decrease in both the maximal photosynthesis (Pmax) and quantum yield of O2 evolution, but Pmax dropped more rapidly than quantum yield at all water deficit conditions tested. However, Chl fluorescence parameters were not affected much. Dehydration did not change the initial fluorescence (Fo) and maximum photochemical efficiency(Fv/Fm) of photosystem(PS) II. Both the photochemical quenching (qP) and non-photochemical quenching(NPQ) were not changed by dehydration under low PFR(50 $\mu$mols m-2s-1 ). In contrast, under high PFR(270$\mu$mols m-2s-1)qP was slightly decreased while NPQ was greatly increased. The fast induction kinetics of Chl fluroecence showed no change in Chl fluorescence pattern by dehydration at high PFR (640 $\mu$mols m-2s-1 ), but exhibited a significant drop in peak level(Fp)at low PRFR (70$\mu$mols m-2s-1 ). PS I oxidation and reduction kinetics revealed normal reduction but delayed oxidation to P-700+, suggesting no lesionin electron flow from PSII to PSI , but impaired electron transport to NADP+,These results suggest that water stress caused by PEG-treatment results in the reduction of photosynthesis, promarily due to the reducted electron trasport from PSI to NADP+ or hampered subsequent steps involving Calvin Cycle.

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Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa

  • Dangol, Sarmina;Singh, Raksha;Chen, Yafei;Jwa, Nam-Soo
    • Molecules and Cells
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    • v.40 no.11
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    • pp.828-836
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    • 2017
  • Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

The effects of psychological factors on elderly of depression scale and Electroephalography

  • Kang, Byeong-Woo;Jung, Mi-Hyun;Cho, Byung-Jun
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.12
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    • pp.157-163
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    • 2016
  • Purpose : This study purposed on the understanding of psychology in the elderly women using depression scale and electroephalography. The subjects were thirty elderly women in geriatric care hospital. All participants were elderly women over 65 years old. The subjects signed up with informed consent and they were divided into a normal elderly group, an exercise elderly group and a hospital elderly group. They were randomly assigned with 10 persons. Methods : The study instruments were Geriatric Depression Scale(GDS) and Poly-G-I. Brain wave activity was measured by 'power spectrum analysis' of TeleScan program. Statistic analysis consisted of average, standard deviation, One-way ANOVA and post-hoc Tukey using SPSS 21.0 version. The significance was set at .05. Results : Depression scale results showed that the exercise elderly group were $10.60{\pm}5.36$ points which was the lowest among the three groups. The Normal elderly group was $16.20{\pm}5.59$, and the hospitalized elderly group was $16.70{\pm}6.76$ points. There was no significant difference between the normal elderly group, exercise elderly group and hospitalized elderly group. The hospitalized group showed statistical difference in relative theta power in the area of Fp1, F3, F4, T3, P3 as compared to the normal elderly group and the exercise elderly groups. There was no significant difference between relative beta power and relative gamma power in three groups. Conclusion : Geriatric depressed scale showed no significant difference in each group. This is indicated in the mental problems associated with depression. This indicates a higher level of depression in the hospitalized elderly women, more than in the normal elderly women and exercise elderly women groups.

Verification Algorithm for the Duplicate Verification Data with Multiple Verifiers and Multiple Verification Challenges

  • Xu, Guangwei;Lai, Miaolin;Feng, Xiangyang;Huang, Qiubo;Luo, Xin;Li, Li;Li, Shan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.15 no.2
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    • pp.558-579
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    • 2021
  • The cloud storage provides flexible data storage services for data owners to remotely outsource their data, and reduces data storage operations and management costs for data owners. These outsourced data bring data security concerns to the data owner due to malicious deletion or corruption by the cloud service provider. Data integrity verification is an important way to check outsourced data integrity. However, the existing data verification schemes only consider the case that a verifier launches multiple data verification challenges, and neglect the verification overhead of multiple data verification challenges launched by multiple verifiers at a similar time. In this case, the duplicate data in multiple challenges are verified repeatedly so that verification resources are consumed in vain. We propose a duplicate data verification algorithm based on multiple verifiers and multiple challenges to reduce the verification overhead. The algorithm dynamically schedules the multiple verifiers' challenges based on verification time and the frequent itemsets of duplicate verification data in challenge sets by applying FP-Growth algorithm, and computes the batch proofs of frequent itemsets. Then the challenges are split into two parts, i.e., duplicate data and unique data according to the results of data extraction. Finally, the proofs of duplicate data and unique data are computed and combined to generate a complete proof of every original challenge. Theoretical analysis and experiment evaluation show that the algorithm reduces the verification cost and ensures the correctness of the data integrity verification by flexible batch data verification.

Forensic Decision of Median Filtering by Pixel Value's Gradients of Digital Image (디지털 영상의 픽셀값 경사도에 의한 미디언 필터링 포렌식 판정)

  • RHEE, Kang Hyeon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.6
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    • pp.79-84
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    • 2015
  • In a distribution of digital image, there is a serious problem that is a distribution of the altered image by a forger. For the problem solution, this paper proposes a median filtering (MF) image forensic decision algorithm using a feature vector according to the pixel value's gradients. In the proposed algorithm, AR (Autoregressive) coefficients are computed from pixel value' gradients of original image then 1th~6th order coefficients to be six feature vector. And the reconstructed image is produced by the solution of Poisson's equation with the gradients. From the difference image between original and its reconstructed image, four feature vector (Average value, Max. value and the coordinate i,j of Max. value) is extracted. Subsequently, Two kinds of the feature vector combined to 10 Dim. feature vector that is used in the learning of a SVM (Support Vector Machine) classification for MF (Median Filtering) detector of the altered image. On the proposed algorithm of the median filtering detection, compare to MFR (Median Filter Residual) scheme that had the same 10 Dim. feature vectors, the performance is excellent at Unaltered, Averaging filtering ($3{\times}3$) and JPEG (QF=90) images, and less at Gaussian filtering ($3{\times}3$) image. However, in the measured performances of all items, AUC (Area Under Curve) by the sensitivity and 1-specificity is approached to 1. Thus, it is confirmed that the grade evaluation of the proposed algorithm is 'Excellent (A)'.