• Journal of Species Research
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• 제12권4호
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• pp.307-312
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• 2023

Oxytricha multilineata, Mixophrya pantanalensis pantanalensis, and Caudiurostyla sinensis were isolated from soil samples collected from Cheongju-si and Yeoju-si, confirmed as new to South Korea. Oxytricha multilineata was distinguished from other congeners by seven dorsal kineties and dorsal bristles about 15 ㎛ long. Mixophrya pantanalensis pantanalensis was characterized by five to seven lithosomes and six dorsal kineties. Caudiurostyla sinensis was characterized by colorless cortical granules present, 10-14 midventral pairs, 7-9 left and 6-9 right marginal rows and four or five dorsal kineties. We determined the ribosomal DNA sequences (including 18S rDNA, ITS1, 5.8S rDNA, ITS2, and partial 28S rDNA) from above three species. And the genetic distances were compared with their congeners.

• Animal Systematics, Evolution and Diversity
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• 제34권3호
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• pp.143-151
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• 2018

A marine ciliate Amphisiella milnei (Kahl, 1932) $Horv{\acute{a}}th$, 1950 was discovered from the tidal pool of Baekdo Island, South Korea. The existence of extra cirri between leftmost frontal cirrus and buccal cirrus discriminates this species from its congeners. Its morphological features are described as follows: body size in vivo $110-130{\times}35-45{\mu}m$; elongate rectangular to elliptical in shape; two large and several small ring-shaped structures; yellowish cortical granules arranged irregularly on ventral side but longitudinally along dorsal kineties on dorsal side; 34-40 adoral membranelles, 3 frontal cirri, 1 buccal cirrus, 1 parabuccal cirrus, usually 2 extra cirri behind leftmost frontal cirrus, and 3 frontoventral cirri; amphisiellid median cirral row composed of 25-31 cirri with 27-36 left and 27-44 right marginal cirri; usually 5 transverse cirri and 2 pretransverse cirri with 7 dorsal kineties; two macronuclear nodules. In addition to, 18S rDNA sequence of A. milnei was analyzed to understand its phylogenetic relationship.

• Animal Systematics, Evolution and Diversity
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• 제36권2호
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• pp.113-122
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• 2020

From a moss sample, we isolated and identified Notohymena gangwonensis Kim et al., 2019 based on morphological and molecular data. The moss and type population has completely identical 18S rRNA (nuclear small subunit ribosomal RNA) gene sequences and both are highly similar in morphological and morphometric attributes, except for the diameter and arrangement of the cortical granules. Thus, we reexamined the type materials(i.e., micrographs and gDNA) and resulted in finding mistakes made by the authors of the species. Based on these data and supporting materials newly obtained (i.e., internal transcribed spacer [ITS] 1, ITS2, 5.8S, and partial 28S rDNA sequences, and scanning electron micrographs), we provide improved diagnosis of the species to clarify its identity. In addition, a key for Notohymena species is provided.

• Animal Systematics, Evolution and Diversity
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• 제19권2호
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• pp.227-235
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• 2003

2002년 제주도의 한 웅덩이에서 채집된 섬모충류를 실험실에서 분리, 배양한 결과 하모류의 이끼토양자라하모충(Euplotes muscorum Dragesco, 1970)으로 동정되었으며 한국에서 처음으로 보고되어 재기재한다. 기재는 생체 표본 관찰, protargol impregnation 그리고 형태통계에 기초하여 기술하였다. 이 종의 형태적 특징은 몸길이 63-78${\mu}m$, 폭 40-52${\mu}m$, 9개의 전방복극모, 5개의 후방극모, 4개의 미극모, 한 개의 대핵과 소핵, 몸길이의 약 2/3을 덮고 있는 막판구대는 32-35개의 구부막판으로 이루어졌고, 8개의 등쪽섬모열, 중앙의 등쪽섬모열의 섬모는 20-24개, 등쪽섬모배열은 complex type 등의 특징들을 가진다. 이 종은 이끼자라하모충(E. muscicola Kahl,1932)과 매우 비슷하지만 이끼토양자라하모충은 8개의 등쪽섬모열, complex type의 등쪽섬모배열을 가지는데 반해 이끼자라하모충는 9개의 등쪽섬모열, multiple type의 등쪽섬모배열을 가지는 형태적 특징을 각각 나타낸다.

• Animal Systematics, Evolution and Diversity
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• 제34권1호
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• pp.37-49
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• 2018

Two hypotrich ciliates, Australocirrus shii (Shi et al., 1997) Kumar & Foissner, 2015 and Afrokeronopsis aurea (Foissner & Stoeck, 2008) Foissner et al., 2010 isolated from freshwater habitats in Korea and were studied based on the specimens from live and after protargol impregnation. Australocirrus shii is redescribed based on morphology and 18S rRNA gene sequence, whereas Af. aurea is the first record for Korea. Main morphological features of the Korean population of Au. shii are as following: body size $100-200{\times}40-80{\mu}m$ in vivo; elongate to ellipsoidal or slightly elongate obovate, dorsoventrally flattened; transverse cirri arranged in (3+2) pattern, anterior pretransverse ventral cirrus distantly anterior of the first transverse cirrus; eight or nine dorsal kineties; and three caudal cirri. Main morphological features of the Korean population of Af. aurea are as following: body size $230-375{\times}70-145{\mu}m$ in vivo; shape elongate obovate or ellipsoidal, widest at the mid-body; undulating membranes in Australocirrus pattern with a buccal depression; and three caudal cirri. The Korean population of A. shii is similar in morphology with previous descriptions except for the presence of indentation at the posterior end in the Korean population. The Korean population of A. aurea is slightly shorter than the South African population and has slightly less marginal and mid-ventral cirri. The phylogenetic analysis of present two Korean hypotrichs and relevant species based on 18S rRNA gene sequences generated almost similar tree topologies compared with previous studies.