• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s01
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• pp.48-54
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• 1989

An immunoassay techniques using monoclonal antibodies have been developed of the quantitative analysis of endogenous hormones in plants. In order to be useful for immunoassay, both a high degree of specificity and a high affinity are required. A system is described for production of hybridomas which secrete antibodies against the plant hormone. Using the system we were able to produce hybridmas with the desired antibody specificity by cell fusion and culture method. For a number of obvious reasons, monoclonal antibodies(mAb) were superior to polyclonal antibodies.

• KSBB Journal
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• v.4 no.2
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• pp.143-149
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• 1989

A cell sedimentaion system was designed and employed for the high culture of hydridoma. An upward divering cell settler allowed a good sedimentation of hybridoma but the accumulation of cell mass on the settler's wall side was a potential prolem. Although a cyinderical cell settler was useful to solve this problem, this device was employable only at low dilytion rate. A modified cell settler could support the high density culture of hybridoma at a concentration of $5{\times}10^6$ cell/ ml during 1 week, producting 380mg of monclonal antibody.