• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.345-351
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• 2004

We investigated the anaerobic ammonium oxidation (anammox) reaction in a lab-stale upflow anaerobic sludge blanket (UASB) reactor. Our aim was to detect and enrich the organisms responsible for the anammox reaction using a synthetic medium that contained low concentrations of substrates (ammonium and nitrite). The reactor was inoculated with granular sludge collected from a full-scale anaerobic digestor used for treating brewery wastewater The experiment was performed during 260 days under conditions of constant ammonium concentration ($50\;mg\;NH_4^+-N/L$) and different nitrite concentrations ($50{\~}150\;mg\;NO_2-N/L$). After 200 days, anammox activity was observed in the system. The microorganisms involved in this anammox reaction were identified as Candidatus B. Anammoxidans and K. Stuttgartiensis using fluorescence in situ hybridization (FISH ) method.

• 생태와환경
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• 제40권4호
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• pp.553-559
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• 2007

The vertical distributions of sulfate reducing bacteria (SRB) in sediments of lakes in Korea (Lake Sihwa and Lake Soyang) and China (Lake Aha and Lake Erhai) were investigated by fluorescence in situ hybridization (FISH). SRB from sediment of Lakes of China were located to deeper layer than those in Lakes of Korea. SRB were not detected below 19 cm and 10 cm depth in sediments of Lake Sihwa and Lake Soyang, respectively. SRB numbers were, however, detected at all observed sediments in Lake Aha and Lake Erhai. In case of lakes in Korea, the proportion of SRB ranged from 2.9 to 25.6% (Lake Sihwa) and ranged from 0.6 to 7.1% (Lake Soyang). For lakes in China, the proportions of SRB were from 0.6 to 19.4% and from 2.9 to 11.2% within sediments from Lake Aha and from Lake Erhai, respectively. The high peaks of SRB numbers in sediments of all lakes were appearing at depths between 0 cm and 2 cm.

• 한국어병학회지
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• 제16권1호
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• pp.61-68
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• 2003

MICs of 8 chemotherapeutic agents against forty streptococcal isolates were determined. These strains were isolated from diseased olive flounder, Paralichthys olivaceus, and showed 2-5 multiple drug resistance against different antibacterial agents including ampicillin, doxycycline (DOXY), erythromycin, florfenicol, flumequine, norfloxacin, oxolinic acid, and oxytetracycline (OTC). In conjugation experiment, we found transferable R plasmids carrying OTC and DOXY resistance determinant in 3 drug resistance strains analyzed. Six out of 40 isolates showed positive signal in colony hybridization with the R plasmid DNA (pST9) as a probe. It suggests that other types R plasmid different from pST9 is also involving in multiple drug resistance of streptococci isolated from olive flounder.

• 한국육종학회지
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• 제43권4호
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• pp.252-259
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• 2011

Conventional staining and fluorescence in situ hybridization (FISH) karyotypes of the non-genetically modified (GM) parental rice line, 'Nakdong' (Oryza sativa L. japonica), and its four GM rice lines, LS28 (event LS30-32-20-1), Cry1Ac1 (event C7-1-9-1), and LS28 ${\times}$ Cry1Ac1 (events L/C1-1-3-1 and L/C1-3-1-1) were analyzed using 5S and 45S rDNAs as probes. Both parental and transgenic lines were diploids (2n=24) with one satellite chromosome pair. The lengths of the prometaphase chromosomes ranged from 1.50 to $6.30{\mu}m$. Four submetacentric and eight metacentric pairs comprised the karyotype of 'Nakdong' and its four GM lines. One pair of 5S rDNA signals was detected near the centromeric region of chromosome g in both the parental and transgenic lines. The 45S rDNA signals were detected on the secondary constrictions of the satellite chromosome pair in both the parental and transgenic lines. There was no significant difference in chromosome size, length, and composition between 'Nakdong' and its four GM lines. This research was conducted as a preliminary study for chromosomal detection of transgenes in GM rice lines and would be useful for their breeding programs.

• 한국약용작물학회지
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• 제12권6호
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• pp.515-518
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• 2004

약용식물로 재배되고 있는 지모를 대상으로 McFISH 기법을 이용하여 45S와 5S rDNA 유전자의 염색체상의 위치를 확인하여 물리지도를 확립하였다. 2쌍의 45S rDNA는 1번 염색체의 단완 말단과 3번 염색체의 동원체 부위에서 관찰되었고, 1번 염색체의 signal이 3번 염색체에서의 signal보다 더 강하게 나타났다. 한 쌍의 5S rDNA signal은 45S rDNA signal과 함께 3번 염색체의 동원체 부위에서 관찰되었다.

• Animal cells and systems
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• 제3권1호
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• pp.69-72
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• 1999

A tandemly repeated DNA sequence was identified and characterized y the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5'-GAAACGGGTG-3', in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C-banded constitutive heterochromatin in the terminal regions of both arms of all 6 chromosomes.

• 대한임상검사과학회지
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• 제42권3호
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• pp.111-115
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• 2010

A 15-year-old female with primary amenorrhea and Tuner's syndrome feature was referred for a chromosome analysis. The karyotype of the patient was 45,X/46,X,der(Y) mosaicism under initial GTG-banding analysis. Fluorescence in situ hybridization (FISH) analysis with probe for CEP X probes and SRY probe (Vysis, Inc. Downers Grove, IL 60515, USA) was carried out. This probe is direct labeled with SpectrumOrange (SRY, Yp11.3) and is available as a single probe or mixed with the CEP X SpectrumGreen probe. SRY SpectrumOrange/CEP X SpectrumGreen hybridized to a specimen obtained from an two isodicentric Y chromosomes. The karyotype of the patient was ish Xcen(DXZ1x1)/Xcen(DXZ1x1), Yp11.3(SRYx2) by using FISH. This karyotype was considered a variant of Tuner syndrome with mixed gonadal dysgenesis (MGD), male pseudohermaphroitism (MPH) and apparently normal male.

• 한국약용작물학회지
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• 제18권3호
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• pp.186-190
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• 2010

The chromosome number was identified and fluorescence in situ hybridization(FISH) mapping of 5S and 45S rDNAs were conducted for C. minima and C. lanceolata in the genus Codonopsis from Jeju island. In this study, we have confirmed that the somatic metaphase chromosome number determined as 2n=2x=16 was the same as the findings from the previous studies. While the conventional staining method makes it rather difficult to distinguish satellite chromosomes due to high degree of variability, FISH analysis produced the exact number and location of 5S and 45S rDNAs. Both species in the genus Codonopsis have a pair of 5S rDNA and their gene loci were observed on chromosome 3. Although two pairs of 45S rDNAs (one on chromosome 1 and the other on chromosome 8) were identified in both species, the 45S rDNA signals on chromosome 8 in C. minima were significantly weaker than those on chromosome 1. In addition, the 45S rDNA signals on chromosome 1 in C. lanceolata showed that the chromosome is non-homologus. In this study, we have determined cytogenetic characteristics of C. minima and C. lanceolata according to their gene replication patterns.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
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• pp.13-15
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• 2004

Telomere는 진핵세포염색체 말단부에 TTAGGG 반복 염기서열을 가지는 DNA-protein 복합체로 세포 분열시마다 짧아지며, 발생 및 노화와 밀접한 관련이 있는 것으로 알려져 있다. 본 연구는 닭에 있어 telomere의 양적 분포양상을 구명함으로써 이를 이용한 개체의 생명표지 (bio-marker)의 가능성을 탐색코자 하였다. 본 분석에 이용된 계종으로는 한국재래계와 단관 백색화이트 레그혼종을 대상으로 하였고, 주령간, 품종간 및 성간 백혈구내 telomere 함량을 비교 분석하였으며, 또한 분석개체들의 생산능력과 이들의 telomere 함유율 간의 상관관계를 조사하였다. Telomere의 양적 분석은 chicken telomeric DNA probe를 이용한 양적 형광접합보인법(Quantitative fluorescence in situ hybridization : Q-FISH)을 이용하였다. Telomere 양적 분석결과. 주령이 증가함에 따라 telomere 함량이 유의적으로 감소됨을 확인하였고, 품종간 및 성간에도 유의적인 차이가 나타났다. 또한 생산능력과 각 개체의 telomere 함량간의 상관분석에 있어 성성숙 일령 및 체중과는 정(+)의 상관을, 산란수 및 난중과는 약한 부(-)의 상관관계를 나타내었다. 이러한 결과는 telomere 함유율이 닭의 생명표지 및 생산능력의 표지로서의 개발 가능성을 시사한다 하겠다.

• 한국물환경학회지
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• 제23권6호
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• pp.920-926
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• 2007

The upflow Biobead$^{(R)}$ process, one of biological aerated filters (BAF), which was used commercially, invented for removal of organic materials and nitrification. This process was modified to enhance the ability of denitrification through the induction of pre-anoxic tank. In this research, we investigated the effects of hydraulic retention time (HRT) and backwashing period in aerobic tank. The characteristics of nitrifying bacteria, which are composed of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), also investigated using fluorescence in situ hybridization (FISH). Even though the HRT was shortened, the efficiency of nitrification was not decreased when the organic loading rate and ammonium-nitrogen loading rate were $2.10kg/m^3/day$ and $0.25kg/m^3/day$, respectively. And then the distribution ratios of AOB and NOB showed the similar patterns. However, when the backwashing period was lengthened from 12 hours to 24 hours in aerobic 1 tank, the nitrification efficiency was decreased to 63.9% from 89.2%. The results of FISH explained that this decrease of nitrification efficiency was caused by the decrease of distribution ratio of AOB in aerobic 1 tank. The nitrification efficiencies of aerobic 1 and aerobic 2 tank were increased when the backwashing period was lengthened because of relative high distribution ratios of nitrifying bacteria.