Zhou, Han;Guo, Xuchao;Liu, Chengqi;Tang, Zhan;Lu, Shuhan;Li, Lin
KSII Transactions on Internet and Information Systems (TIIS)
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v.15
no.11
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pp.3991-4010
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2021
The Question Similarity Measurement of Chinese Crop Diseases and Insect Pests (QSM-CCD&IP) aims to judge the user's tendency to ask questions regarding input problems. The measurement is the basis of the Agricultural Knowledge Question and Answering (Q & A) system, information retrieval, and other tasks. However, the corpus and measurement methods available in this field have some deficiencies. In addition, error propagation may occur when the word boundary features and local context information are ignored when the general method embeds sentences. Hence, these factors make the task challenging. To solve the above problems and tackle the Question Similarity Measurement task in this work, a corpus on Chinese crop diseases and insect pests(CCDIP), which contains 13 categories, was established. Then, taking the CCDIP as the research object, this study proposes a Chinese agricultural text similarity matching model, namely, the AgrCQS. This model is based on mixed information extraction. Specifically, the hybrid embedding layer can enrich character information and improve the recognition ability of the model on the word boundary. The multi-scale local information can be extracted by multi-core convolutional neural network based on multi-weight (MM-CNN). The self-attention mechanism can enhance the fusion ability of the model on global information. In this research, the performance of the AgrCQS on the CCDIP is verified, and three benchmark datasets, namely, AFQMC, LCQMC, and BQ, are used. The accuracy rates are 93.92%, 74.42%, 86.35%, and 83.05%, respectively, which are higher than that of baseline systems without using any external knowledge. Additionally, the proposed method module can be extracted separately and applied to other models, thus providing reference for related research.
This paper considers the combination of cyclic and axial loads to investigate the hysteretic performance of H-section 6061-T6 aluminum alloy members. The hysteretic performance of aluminum alloy members is the basis for the seismic performance of aluminum alloy structures. Despite the prevalence of aluminum alloy reticulated shells structures worldwide, research into the seismic performance of aluminum alloy structures remains inadequate. To address this deficiency, we design and conduct cyclic axial load testing of three H-section members based on a reliable testing system. The influence of slenderness ratios and bending direction on the failure form, bearing capacity, and stiffness degradation of each member are analyzed. The experiment results show that overall buckling dominates the failure mechanism of all test members before local buckling occurs. As the load increases after overall buckling, the plasticity of the member develops, finally leading to local buckling and fracture failure. The results illustrate that the plasticity development of the local buckling position is the main reason for the stiffness degradation and failure of the member. Additionally, with the increase of the slenderness ratio, the energy-dissipation capacity and stiffness of the member decrease significantly. Simultaneously, a finite element model based on the Chaboche hybrid strengthening model is established according to the experiment, and the rationality of the constitutive model and validity of the finite element simulation method are verified. The parameter analysis of twenty-four members with different sections, slenderness ratios, bending directions, and boundary conditions are also carried out. Results show that the section size and boundary condition of the member have a significant influence on stiffness degradation and energy dissipation capacity. Based on the above, the appropriate material constitutive relationship and analysis method of H-section aluminum alloy members under cyclic loading are determined, providing a reference for the seismic design of aluminum alloy structures.
This study examines the two-level behavior of the toggle brace damper within a steel frame having a yielding pipe damper and rotational friction damper. The proposed system has two kinds of fuse for energy dissipation in two stages. In this mechanism, rotational friction damper rather than hinged connection is used in toggle brace system, connected to a pipe damper with a limited gap. In order to create a gap, bolted connection with the slotted hole is used, such that first a specific movement of the rotational friction damper solely is engaged but with an increase in movement, the yielding damper is also involved. The performance of the system is such that at the beginning of loading the rotational friction damper, as the first fuse, absorbs energy and with increasing the input load and further movement of the frame, yielding damper as the second fuse, along with rotational friction damper would dissipate the input energy. The models created by ABAQUS are subjected to cyclic and seismic loading. Considering the results obtained, the flexibility of the hybrid two-level system is more comparable to the conventional toggle brace damper. Moreover, this system sustains longer lateral displacements. The energy dissipation of these two systems is modeled in multi-story frames in SAP2000 software and their performance is analyzed using time-history analysis. According to the results, permanent relocations of the roof in the two-level system, in comparison with toggle brace damper system in 2, 5, and 8-story building frames, in average, decrease by 15, 55, and 37% respectively. This amount in a 5-story building frame under the earthquakes with one-third scale decreases by 64%.
The purpose of this study is to propose a conceptual model for the tourism application of the block chain consensus construct and to test the intention of technology acceptance of potential consumers. First, we have tried to investigate the security, reliability, functionality was derived. Secondary, structural validation of the proposed model confirmed the factors influencing the acceptance of block chain technology in terms of consumers. Based on this, we apply the Unified Theory of Acceptance and Use of Technology to evaluate. Individual innovation and block-chain technology have a strong causal relationship with the proposed block chain acceptance intentions based on the Hybrid Block Chain Consensus system, which shows strong innovation and strong cognitive status. In addition, the factors directly affecting the acceptance of block-chain are the benefits expected from the block chain, the technical infrastructure required to use the service, the perceived benefits available. The influence of the surrounding environment on the adoption of technology and ease of use on new technology did not affect the acceptance intention significantly.
In order to achieve efficiency of laying eggs of silkworms, it is very important to eliminate noncocooning silkworms. This study is written on the basis of observation and analysis of mechanism of silkworms physiologically and anatomically. It is hoped that given herein will contribute to the effecting elimination work. Outline of the study summarize as follows: 1. It is observed through microscope that the silkworms which are seen normal state in the silk gland but no ability of cocoon making have polyhedrosis in the nerve, trachea and muscle near the tissue of the spinneret. 2. Relatively high proportion of non-cocooning silkworms are caused by the grasserie of the silkworms. 3. As a result of inoculation with purulent discharges against silkworms from first fooding through 8th day of 5th instar, number of cocooning silkworms were increased when inoculation are applied at laterer day of the instar. In the case of non-cocooning silkworms, meanwhile, resulted not big varriation when it is apllied in the early and middle of the period, but number of non-cocooning silkworm was reduced when the inoculation are given at laterer of the instar. Number of death during rearing and mounting are increased when earliest application of inoculation are carried out. 4. Symptom of grasserie was appeared more or less three days after application of the inoculation. Some silkworms which were inoculated just before mounting has ability of cocooning making even taken grasserie, in this case the silkworm can make thin cocoon. since the silkworm fall sick during cocoon making and unable of spinning soon. when the worm was affected by grasserie slightly, it was observed that the silkworm can spinning. It is supposed to be the light paralysis of spinneret is not very much influenced to spinning. 5. As a result of observation of original stock and hybrid including other 44 kinds of silkworm, many non-cocooning silkworms were found in the original stock especially originated from japanese than in hybrid. 6. A number of undulations are found in the middle division of the silk gland of non-cocooning silkworms. 7. According to the observation of the sizes of the body and digestive organs, normal natured silkworms and non-cocooning silkworms are more or less same in length, but the width, circumference of bodies and digestive organs were more larger in the later. If silkworm which was in the period of active eating of 5th instar was given shock of dropping to the floor, the silkworm receives little more shock when hit to side of the body than to head, and concrete floor than ondol and wooden floor.
Jang, Won Hee;Jeong, Young Joo;Choi, Sun Hee;Kim, Sang-Jin;Urm, Sang-Hwa;Moon, Il Soo;Seog, Dae-Hyun
Journal of Life Science
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v.24
no.12
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pp.1276-1283
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2014
Cell adhesion molecules determine the cell-cell binding and the interactions between cells and extracellular signals. Cell-cell junctional complexes, which maintain the structural integrity of tissues, consist of more than 50 proteins including multi-PDZ domain protein 1 (MUPP1). MUPP1 contains 13 postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domains and serves a scaffolding function for transmembrane proteins and cytoskeletal proteins or signaling proteins, but the mechanism how MUPP1 links and stabilizes the juxtamembrane proteins has not yet been elucidated. We used the yeast two-hybrid system to identify proteins that interact with PDZ domains of MUPP1. We found an interaction between MUPP1 and cell adhesion molecule 1 (Cadm1, also known as SynCAM1, Necl-2, or TSLC1). Cadm1 bound to the second PDZ domain of MUPP1. The carboxyl (C)-terminal end of Cadm1 has a type II PDZ-association motif (-Y-F-I) which was essential for the interaction with MUPP1 in the yeast two-hybrid assay. MUPP1 also bound to the C-terminal cytoplasmic tail region of other Cadm family members (Cadm2, Cadm3, and Cadm4). In addition, these protein-protein interactions were observed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti-MUPP1 antibody co-immunoprecipitated Cadm1 and Cadm4 with MUPP1 from mouse brain extracts. These results suggest that MUPP1 could mediate interaction between Cadms and cytoskeletal proteins.
Jang, Won Hee;Jeong, Young Joo;Choi, Sun Hee;Lee, Won Hee;Kim, Mooseong;Kim, Sang-Jin;Urm, Sang-Hwa;Moon, Il Soo;Seog, Dae-Hyun
Journal of Life Science
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v.24
no.8
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pp.820-826
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2014
The localization to specific subcellular sites and the regulation of cell surface receptors and channels are crucial for proper functioning. Postsynaptic density-95/Disks large/Zonula occludens-1 (PDZ)-domain is involved in recognition of and interaction between various proteins, by which the localization and the regulation are mediated. Multi-PDZ domain protein 1 (MUPP1) contains 13 PDZ domains. MUPP1 serves a scaffolding function for structure proteins and signaling proteins, but the mechanism how MUPP1 is stabilized and signalized has not yet been elucidated. We used the yeast two-hybrid system to identify proteins that interact with PDZ domains of MUPP1. We found an interaction between MUPP1 and Parkin. Parkin is an E3 ubiquitin ligase. Loss-of-function mutations of Parkin gene are known to cause an autosomal recessive juvenile parkinsonism. Parkin bound to the $12^{th}$ PDZ domain, but not to other PDZ domains of MUPP1. The C-terminal end of Parkin has a type II PDZ-association motif, which was essential for the interaction with MUPP1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, Parkin co-localized with MUPP1. When co-expressed with ubiquitin in HEK-293T cells, MUPP1 has been strongly ubiquitinated by Parkin. These findings collectively suggest that MUPP1 is a novel substrate of Parkin and its function or stability could be modulated by Parkin-mediated ubiquitination.
The purpose of this study was to evaluate the effect of instrument compliance on the polymerization shrinkage stress measurements of dental composites. The contraction strain and stress of composites during light curing were measured by a custom made stress-strain analyzer, which consisted of a displacement sensor, a cantilever load cell and a negative feedback mechanism. The instrument can measure the polymerization stress by two modes: with compliance mode in which the instrument compliance is allowed, or without compliance mode in which the instrument compliance is not allowed. A flowable (Filtek Flow: FF) and two universal hybrid (Z100: Z1 and Z250: Z2) composites were studied. A silane treated metal rod with a diameter of 3.0 mm was fixed at free end of the load cell, and other metal rod was fixed on the base plate. Composite of 1.0 mm thickness was placed between the two rods and light cured. The axial shrinkage strain and stress of the composite were recorded for 10 minutes during polymerization. and the tensile modulus of the materials was also determined with the instrument. The statistical analysis was conducted by ANOVA. paired t-test and Tukey's test (${\alpha}<0.05$). There were significant differences between the two measurement modes and among materials. With compliance mode, the contraction stress of FF was the highest: 3.11 (0.13). followed by Z1: 2.91 (0.10) and Z2: 1.94 (0.09) MPa. When the instrument compliance is not allowed, the contraction stress of Z1 was the highest: 17.08 (0.89), followed by FF: 10.11 (0.29) and Z2: 9.46 (1.63) MPa. The tensile modulus for Z1, Z2 and FF was 2.31 (0.18), 2.05 (0.20), 1.41 (0.11) GPa, respectively. With compliance mode. the measured stress correlated with the axial shrinkage strain of composite: while without compliance the elastic modulus of materials played a significant role in the stress measurement.
Bax, a mammalian pro-apoptotic member of the Bcl-2 family induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorhodamine123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MSP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to playa novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.
In spite of various merit of algae as biofuel, the production cost of algae is a considerable obstacle for commercialization. The concurrent development of essential technologies is needed for the cultivating, harvesting, extracting and energy transformation. The production cost of algae biofuel has still higher than that of the other commercial biofuel. The major research activity has been focused on the cultivating and the research of other processes has been done with relatively lower activity. It is difficult to separate the algae from water because of the similar magnitude of density each other. The agglomeration and extracting of algae with the hybrid technology using ultrasonic wave is rare effect of environmental hazard and also it is appropriate technology for the next generation energy resources. The present research is investigated for the effective separation of algae from water with the ultrasonics wave. The aim of the present research is focused on the establishment of optimal design of algae agglomeration system. For this purpose, the computational fluid dynamic analysis has been conducted in the flow field with ultrasonic wave and algae flow to clarify the mechanism of algae separation by ultrasonic wave.
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