• Development and Reproduction
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• v.12 no.3
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• pp.289-296
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• 2008

BPES (Blepharophimosis/Ptosis/Epicanthus inversus Syndrome) is an autosomal dominant disorder caused by mutations in FOXL2. Affected individuals have premature ovarian failure (POF) in addition to small palpebral fissures, drooping eyelids, and broad nasal bridge. FOXL2 is a member of the forkhead family transcription factors. In FOXL2-deficient ovaries, granulosa cell differentiation dose not progress, leading to arrest of folliculogenesis and oocytes atresia. Using yeast two-hybrid screening of rat ovarian cDNA library with FOXL2 as bait, we found that small ubiquitin-related modifier (SUMO)-conjugating E2 enzyme UBE2I protein interacted with FOXL2 protein. UBE2I also known as UBC9 is an essential protein for processing SUMO modification. Sumoylation is a form of post-translational modification involved in diverse signaling pathways including the regulation of transcriptional activities of many transcriptional factors. In the present study, we confirmed the protein-protein interaction between FOXL2 and UBE2I in human cells, 293T, by in vivo immunoprecipitation. In addition, we generated truncated FOXL2 mutants and identified the region of FOXL2 required for its association with UBE2I using yeast-two hybrid system. Therefore, the identification of UBE2I as an interacting protein of FOXL2 further suggests a presence of novel regulatory mechanism of FOXL2 by sumoylation.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.48 no.2
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• pp.106-116
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• 2011

USN(Ubiquitous Sensor Network) environment permits us to access whatever information we want, whenever we want. The technologies to provide a basement to these environments premise an accurate location establishment. Especially, ITS(Intelligent Transportation Systems) is easily constructed by applying USN technology. Localization can be categorized as either Range-based or Range-free. Range-based is known to be not suitable for the localization based on sensor network, because of the irregularity of radio propagation and the additional device requirement. The other side, Range-free is much appropriated for the resource constrained sensor network because it can actively locate by means of the communication radio. But, generally the location accuracy of Range-free is low. Especially, it is very low in a low-density environment. So, these two methods have both merits and demerits. Therefore, it requires a new method to be able to improve tracking accuracy by combining the two methods. This paper proposes the tracking scheme based on range-hybrid, which can markedly enhance tracking accuracy by effectively using the information of surrounding nodes and the RSSI(Received Signal Strength Indication) that does not require additional hardware. Additionally, we present a method, which can improve the accuracy of vehicle tracking by adopting the prediction mechanism. Simulation results show that our method outperforms other methods in the transportation simulation environment.

• Journal of the Earthquake Engineering Society of Korea
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• v.8 no.4
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• pp.51-62
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• 2004

Fuzzy supervisory control technique for the seismic response control of cable-stayed bridges subject to earthquakes is studied. The proposed technique is a hybrid control method, which adopts a hierarchical structure consisting of several sub-controllers and a fuzzy supervisor. Sub-controllers are independently designed to reduced the responses to be controlled of a cable-stayed bridge, and a fuzzy supervisor achieves improved seismic control performance by tuning the pre-designed sub-controllers. It is realized by converting static gains of the sub-controllers into time-varying dynamic gains through the fuzzy inference mechanism. To evaluate the feasibility of the proposed technique, the benchmark control problem of cable-stayed bridge proposed by Dyke et al. is adopted. The control variables for the seismic response control of the cable-stayed bridge are determined to be t도 shear forces and bending moments at the base of the towers, the longitudinal displacements at the top of the towers, the relative displacements between the deck and the tower, and the tensions in the stay cables. Comparative results between the fuzzy supervisory controller and LQG controller demonstrate the effectiveness of the proposed control technique.

• BMB Reports
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• v.42 no.4
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• pp.232-237
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• 2009

Sterol regulatory element-binding protein (SREBP)-1c plays a crucial role in the regulation of lipogenic enzymes in the liver. We previously reported that an X-chromosome-linked RNA binding motif (RBMX) regulates the promoter activity of Srebp-1c. However, still unknown was how it regulates the gene expression. To elucidate this mechanism, we screened the cDNA library from mouse liver by yeast two-hybrid assay using RBMX as bait and identified scaffold attachment factor B1 (SAFB1). Immunoprecipitation assay demonstrated binding of SAFB1 to RBMX. Chromatin immunoprecipitation assay showed binding of both SAFB1 and RBMX to the upstream region of Srebp-1c gene. RNA interference of Safb1 reduced the basal and RBMX-induced Srebp-1c promoter activities, resulting in reduced Srebp-1c gene expression. The effect of SAFB1 overexpression on Srebp-1c promoter was found only in the presence of RBMX. These results indicate a major role for SAFB1 in the activation of Srebp-1c through its interaction with RBMX.

• Journal of KIISE:Information Networking
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• v.31 no.1
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• pp.11-19
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• 2004

P2P network environments provide users with direct data transmission and sharing facilities and those environments can be classified into hybrid P2P network environments and pure P2P network environments according to the arbitration mechanism among the peers in the network. In hybrid P2P network environments, there exists a server that maintains index information for the data to be shared and network isolation does not occur because every peer always keeps connection to the server. In pure P2P network environments, however, each peer directly connects to another peer and gets services without server intervention, and so, network isolation can occur when the mediating peer fails to work. In this paper. we propose a scheme for each peer to keep connection to other peers continuously by maintaining IP addresses of its neighbor peers and connecting to the peers when the mediating peer fails to work. Although the P2P application that uses our proposed framework should obtain one or more IP addresses of the neighbor peers manually, after instantiation, the application can do its job while maintaining connection to the network continuously and automatically. To evaluate our proposed scheme, we measured and analyzed the time for a peer to reconnect to the network when the mediating peer fails and the network isolation occurs.

• Journal of Mushroom
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• v.5 no.1
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• pp.34-38
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• 2007

The white-colored and the dark gray-colored mutants were frequently happened in cultivated areas of Pleurotus ostreatus (Wonhyeong-neutari). These caused conflicts between farmers and spawn companies. Our studies were conducted to elucidate the mechanism of mutagenesis. The results from the studies would provide valuable informations that could be used to prevent the color-related mutation, and also will be applied in breeding programs of P. ostreatus. Oyster mushroom variety, Wonhyeong-neutari, is somatic hybrid of Pleurotus and has genetic makers for arginine, ornithine, proline, riboflavine. Genetic markers analysis of monospore isolates derived from color mutants show identical tendency with that of Wonhyeong-neutari, these results indicate that color mutants were derived from Wonhyeongneutari. Twenty-one and four homokaryons were selected from the white-colored mutant MGL 2205 and gray-colored ASI 2029. All 34 F1 hybrids derived from the white-colored mutant MGL 2205 produce white-color fruiting bodies, indicating that the white color trait is heritable. In the first generation hybrids between the white-colored MGL 2205 and the gray-colored ASI 2029, all 16 hybrids produced pigmented fruiting bodies. Homokaryons isolated from the hybrid MGL 2205 X ASI 2029 were mated with homokaryon tester strains derived from MGL 2205. By these result, we could assumed that white color trait is a heritable character which is controlled by more than one recessive gene.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1063-1067
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• 2007

The Bcl-2 family proteins play critical roles in regulation of apoptosis, and the balanced interaction of pro- and anti-death members is a key factor in determining the cell fate. Noxa, a BH3-only Bcl-2-family member, has been originally identified as a target gene of p53. To understand the mechanism by which human Noxa (hNoxa) regulates the cell death, we screened the hNoxa binding partner using the yeast two hybrid screening and found that anti-death protein Mcl-1 binds to hNoxa. The binding of hNoxa to Mcl-1 was confirmed by immunoprecipitation in human colon cancer cell line HCT 116 cells. Mcl-1 significantly inhibited the hNoxa-induced cell death in HCT 116 cells. During the cell death induced by hNoxa, Mcl-1 protein was degraded. Its degradation was inhibited by z-VAD-fmk, a pancaspase inhibitor, suggesting caspase is responsible for Mcl-1 degradation in response to hNoxa. Together, the results indicate that hNoxa binds to Mcl-1 that is degraded by cas-pases during hNoxa-induced cell death.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.9
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• pp.691-698
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• 2006

TCP/IP Offload Engine (TOE) is a technology that processes TCP/IP on a network adapter instead of a host CPU to reduce protocol processing overhead from the host CPU. There have been some approaches to implementing TOE: software TOE based on an embedded processor; hardware TOE based on ASIC implementation; and hybrid TOE in which software and hardware functions are combined. In this paper, we designed software modules and hardware modules for a hybrid TOE on an FPGA that had two processor cores. Software modules are based on the embedded Linux. Hardware modules are for data transmission (TX) and reception (RX). One core controls the TX path and the other controls the RX path of the Linux. This TX/RX path separation mechanism can reduce task switching overheads between processes and overcome poor performance of single embedded processor. Hardware modules deal with creating headers for outgoing packets, processing headers of incoming packets, and fetching or storing data from or to the host memory by DMA. These can make it possible to improve the performance of data transmission and reception. We proved performance of the TOE with separated transmission and reception paths by performing experiments with a TOE network adapter that was equipped with the FPGA having processor cores.

• Journal of the Korea Concrete Institute
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• v.21 no.5
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• pp.589-597
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• 2009

High Performance Fiber-reinforced Cement Composite (HPFRCC) shows the multiple crack and damage tolerance capacity due to the interfacial bonding of the fibers to the cement matrix. For practical application, it is needed to investigate the fractural behavior of HPFRCC and understand the micro-mechanism of cement matrix with reinforcing fiber. This study is devoted to the investigation of the AE signals in HPFRCC under monotonic and cyclic uniaxial compressive loading, and total four series were tested. The major experimental parameters include the type and volume fraction of fiber (PE, PVA, SC), the hybrid type and loading pattern. The test results showed that the damage progress by compressive behavior of the HPFRCC is a characteristic for the hybrid fiber type and volume fraction. It is found from acoustic emission (AE) parameter value, that the second and third compressive load cycles resulted in successive decrease of the amplitude as compared with the first compressive load cycle. Also, the AE Kaiser effect existed in HPFRCC specimens up to 80% of its ultimate strength. These observations suggested that the AE Kaiser effect has good potential to be used as a new tool to monitor the loading history of HPFRCC.

• Journal of the Korea Society of Computer and Information
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• v.16 no.6
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• pp.131-142
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• 2011

In this paper, we propose a new multipath routing protocol and compare it with other multipath routing protocols in mobile ad hoc network (MANET) environments. The new multipath routing establishes the main route using a mechanism based on Ad Hoc On-demand Distance Vector(AODV), after which data transmission starts immediately. The backup route search process takes place while data are transmitted, to reduce the transmission delay. The protocol can also operate in a hybrid node-disjoint/link-disjoint mode, where the protocol finds the node-disjoint backup route first; if the node-disjoint does not exist, the protocol discovers the link-disjoint backup route from the main route. When either of the main route or the backup route is broken, data are transmitted continuously through the other route and the broken route is recovered by the route maintenance process. The results of simulations, based on the Qualnet simulator, show that the proposed multipath routing protocol increases the packet transmission success rate and reduces end-to-end delays, when compared with AODV and AOMDV protocols.