• Journal of Veterinary Clinics
• /
• v.32 no.3
• /
• pp.224-230
• /
• 2015

This study was designed in the fabricated poly (L-Lactic-co-${\varepsilon}$-Caprolactone) (PLCL) scaffold using chitosan-alginate hydrogel, which would be more suitable to maintain the biological and physiological functions continuing three dimensional spatial organizations for chondrocytes. As a scaffold, hydrogels alone is weak at endure complex loading within the body. In this study, we made cell hybrid scaffold constructs with poly (L-Lactic-co-${\varepsilon}$-Caprolactone) (PLCL) scaffold and hydrogels to make a three-dimensional composition of cells and extracellular matrix, which would be a mimic of a native cartilage. Using a particle leaching technique with NaCl, we fabricated a highly-elastic scaffold from PLCL with 85% porosity and $300-500{\mu}m$ pore size. A mixture of bovine chondrocytes and chitosan-alginate gel was seeded and compared with alginate as a control on the PLCL scaffold. The cell maturation, proliferation, extracellular matrix synthesis, glycosaminoglycans (sGAG) production and collagen type-II expressions were better in chondrocytes seeded in chitosan-alginate hydrogel than in alginate only. These results indicate that chondrocytes with chitosan-alginate gel on PLCL scaffolds provide an appropriate biomimetic environment for cell proliferation and matrix synthesis, which could successfully be used for cartilage repair and regeneration.

• The Journal of the Korea Contents Association
• /
• v.12 no.9
• /
• pp.194-201
• /
• 2012

The expansion of media is in close connection with the expansion of awareness. The invention of characters enabled mankind to cross over time and space. Machines led to the development of body functions and electricity led to the expansion of space and time. Computers are the extension of the human brain and the advent of the internet led to the expansion of relationships. Even at this moment, media is unremittingly progressing like a spread of a mutant virus, and has resulted in fusion and complex phenomena such as convergence and hybrid media. Transmedia is a compound word formed by the word "Trans" which means traverse, transcend, penetrate or change, and the word "Media" and has the meaning "media which transcends media" which embraces all of modern day media. However, unlike other fusion or complex media, it is different in that it is not a combination of technologies but a combination of technology and emotion. Thus, transmedia should be recognized as a form of media that carries a significant meaning from the user experience aspect as it must simultaneously satisfy both "emotional awareness", which appeals to the human emotion, and "conscious awareness" of mankind, which arises out of the digital technology considered to be important in the smart-era society. This study first examines the concept of transmedia, and then examines the role of user experience design which triggers conscious thinking and strategies for emotional marketing. This study aims to be recognized as a matter for consideration with respect to the development stage for the establishment of a steady communication relationship between developers and designers, as well as communication with users.

• Molecules and Cells
• /
• v.40 no.3
• /
• pp.230-242
• /
• 2017

In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. However, only a few of them have been characterized, and the functions of most MAP3Ks are largely unknown. In this paper, we report the function of MAP3K16 and several other MAP3Ks, MAP3K14/15/17/18, whose expression is salt-inducible. We prepared MAP3K16 overexpression (OX) lines and analyzed their phenotypes. The result showed that the transgenic plants were ABA-insensitive during seed germination and cotyledon greening stage but their root growth was ABA-hypersensitive. The OX lines were more susceptible to water-deficit condition at later growth stage in soil. A MAP3K16 knockout (KO) line, on the other hand, exhibited opposite phenotypes. In similar transgenic analyses, we found that MAP3K14/15/17/18 OX and KO lines displayed similar phenotypes to those of MA3K16, suggesting the functional redundancy among them. MAP3K16 possesses in vitro kinase activity, and we carried out two-hybrid analyses to identify MAP3K16 substrates. Our results indicate that MAP3K16 interacts with MKK3 and the negative regulator of ABA response, ABR1, in yeast. Furthermore, MAP3K16 recombinant protein could phosphorylate MKK3 and ABR1, suggesting that they might be MAP3K16 substrates. Collectively, our results demonstrate that MAP3K16 and MAP3K14/15/17/18 are involved in ABA response, playing negative or positive roles depending on developmental stage and that MAP3K16 may function via MKK3 and ABR1.

• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.31 no.1
• /
• pp.89-113
• /
• 2020

Libraries are one of the essential living infrastructures in urban regeneration. Beyond its meaning as a basic living infrastructure, it is necessary to first understand the role, nature and forms of participation in existing urban regeneration projects in order for libraries to perform their functions and roles effectively as participants of urban regeneration. Therefore, this study investigates the contents related to libraries in the contents of the strategic plan related to urban regeneration projects, and considers them in terms of the role of the library and the form of participation. Through this, the city's library would seek to maximize its role and function as a participant in urban regeneration. As a result, the library in urban regeneration is expected to improve the quality of living environment, Urban vitality, culturally, educationally, socially/welfare role closely related to life, and the role of community as a hub. In addition, the types of library participation could be largely divided into regional and geographical libraries, hybrid libraries combined with various services, libraries based on community cooperation and linkage, and regional community hub libraries based on community participation. In the future, the library needs to promote a more active role change based on the five roles expected as participants in urban regeneration. In addition, more detailed discussions on librarians, services, and programs, which are the main subjects of library services, will be needed in developing urban regeneration plans in order for libraries to successfully fulfill their role as urban regeneration centers.

• Journal of the Korean Institute of Gas
• /
• v.7 no.4 s.21
• /
• pp.44-52
• /
• 2003

In this work dynamic heat transfer in a CPFS (cable penetration fire stop) system built in the firewall of nuclear power plants is three-dimensionally investigated to develop a test-simulator that can be used to verify effectiveness of the sealant. Dynamic heat transfer in the fire stop system is formulated in a parabolic PDE (partial differential equation) subjected to a set of initial and boundary conditions. First, the PDE model is divided into two parts; one corresponding to heat transfer in the axial direction and the other corresponding to heat transfer on the vertical planes. The first PDE is converted to a series of ODEs (ordinary differential equations) at finite discrete axial points for applying the numerical method of SOR (successive over-relaxation) to the problem. The ODEs are solved by using an ODE solver In such manner, the axial heat flux can be calculated at least at the finite discrete points. After that, all the planes are separated into finite elements, where the time and spatial functions are assumed to be of orthogonal collocation state at each element. The initial condition of each finite element can be obtained from the above solution. The heat fluxes on the vertical planes are calculated by the Galerkin FEM (finite element method). The CPFS system was modeled, simulated, and analyzed here. The simulation results were illustrated in three-dimensional graphics. Through simulation, it was shown clearly that the temperature distribution was influenced very much by the number, position, and temperature of the cable stream, and that dynamic heat transfer through the cable stream was one of the most dominant factors, and that the feature of heat conduction could be understood as an unsteady-state process.

• The Korean Journal of Mycology
• /
• v.49 no.4
• /
• pp.507-514
• /
• 2021

Lentinula edodes have been cultivated extensively not only in South Korea but also in other Asian countries. In terms of taste and nutrition, it is a valuable mushroom that is comparable to other mushrooms. L. edodes contains various physiologically active compounds that promote human health. One compound, ergothioneine, exerts a powerful antioxidant function that inhibits cellular senescence. As it is not biosynthesized by plants or animals, L. edodes is very important for ergothioneine supply. The L. edodes cultivars Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420) of the National Institute of Forest Science (NIFoS) had higher ergothioneine content than the other cultivars, and 11 strains were obtained using mono-mono hybridization between them. The strains were inoculated into sawdust media to obtain fruiting bodies, and the ergothioneine content of each hybrid strain was confirmed using high-performance liquid chromatography (HPLC) analysis. Among the 11 strains, NIFoS 5108 had a higher ergothioneine content than the parental strains, Bambithyang (NIFoS 3404) and Sansanhyang (NIFoS 3420). This study revealed that it is possible to develop cultivars with improved specific functions using mono-mono hybridization.

• The Journal of the Korea Contents Association
• /
• v.18 no.12
• /
• pp.646-659
• /
• 2018

This study proposed "Reservoir Park", which added disaster prevention function to urban green spaces such as city parks through domestic and overseas related laws review, case studies, field trips. This is a combination of urban parks and reservoirs as urban planning facilities, which can provide both space for daily use by urban residents and disaster mitigation functions in case of emergency. In order to prevent flooding in urban areas due to climate change, facilities should be installed in the form of parks, etc., as the reservoir facility should be systematically reviewed together with urban planning facilities. However it was found that the reservoir park was not clear as a theme park. In this study, the types of storage facilities in urban areas were reclassified into five types of storage parks reflecting the characteristics of urban green spaces through domestic case studies and field trips. The classification of the reservoir parks is classified into 5 kinds such as ecological type, vegetation cover type, exercise facility type, underground burial type and hybrid type based on groundwater level, human use, and reservoir size. This classification system can be used to determine the types of facilities to be built after designating the location of future storage facilities.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.42 no.1
• /
• pp.27-40
• /
• 2014

The functions of urban open space, which embraces community revitalization, are diverse. It is the means of public healthcare, learning centers for children, hub of arts and cultural programs, as well as promoter of urban tourism. However, in-depth discourse and research on the topic of urban open spaces has been limited so far. Hence, this study aims to investigate the motivations and satisfaction of visitation based on four representative waterfront open space in Seoul; Cheongyecheon Waterfront, Seoul Forest Park, Seonyudo Park and Banpo Hangang Park. The methods of study are literature review, observation investigation, and questionnaire survey. The findings are analyzed through the Exploratory Factor Analysis, Reliability Analysis, ANOVA Analysis and Regression Analysis by SPSS 18.0. The results of the study are as follows. First, urban waterfront open spaces in Seoul has 5 factors of visitor motivation; community amenity, nature access, cultural and educational assets, aesthetic enjoyment, and lastly means of escape. Second, factors of recognizing urban waterfront open spaces as community amenity and nature access indicate meaningful differences in visitor's perception by spatial characteristics. Third, distances between the destination and the visitor's residence influence significantly their perceived motivation. Close-range visitors perceived nature access as a principal factor, whilst medium to long-range visitors perceived visitation for aesthetic purposes more importantly. Lastly, the will to escape was shown as the influential factor in visitor satisfaction. Visiting open spaces for the enjoyment of nature and aesthetic purposes were factors that also closely relate to visitor satisfaction. In addition, it was found that there are different visitor motivations that influence visitor satisfaction in accordance with the spatial characteristics of each open space. In summary, it can be said that urban waterfront open space is a hybrid space connected to various types of urban contents beyond daily experiences. It was found that several visitor motivations including community development, design aesthetics, education and culture, entertainment, enjoyment of natural landscape, and relaxation, affect the overall satisfaction of the visiting experience. It is anticipated that the results of the study will be used by the local government in setting up strategies for the creation and management of successful urban waterfront open space, and for those involved in planning and design act as a starting point for spatial programming and amenities arrangement in accordance to the city's tourism and urban marketing approach.

• Korean Journal of Community Nutrition
• /
• v.26 no.5
• /
• pp.327-336
• /
• 2021

Objectives: This study was conducted to create a 3D printable snack dish model for the elderly with low food or fluid intake along with barriers towards eating. Methods: The decision was made by the hybrid-brainstorming method for creating the 3D model. Experts were assigned based on their professional areas such as clinical nutrition, food hygiene and chemical safety for the creation process. After serial feedback processes, the grape shape was suggested as the final model. After various concept sketching and making clay models, 3D-printing technology was applied to produce a prototype. Results: 3D design modeling process was conducted by SolidWorks program. After considering Dietary reference intakes for Koreans (KDRIs) and other survey data, appropriate supplementary water serving volume was decided as 285 mL which meets 30% of Adequate intake. To consider printing output conditions, this model has six grapes in one bunch with a safety lid. The FDM printer and PLA filaments were used for food hygiene and safety. To stimulate cognitive functions and interests of eating, numbers one to six was engraved on the lid of the final 3D model. Conclusions: The newly-developed 3D model was designed to increase intakes of nutrients and water in the elderly with dementia during snack time. Since dementia patients often forget to eat, engraving numbers on the grapes was conducted to stimulate cognitive function related to the swallowing and chewing process. We suggest that investigations on the types of foods or fluids are needed in the developed 3D model snack dish for future studies.

• Journal of Life Science
• /
• v.33 no.7
• /
• pp.531-537
• /
• 2023

Intracellular and axonal transport is mediated by microtubule-dependent motor proteins, such as kinesins and cytoplasmic dynein. Kinesin moves along the microtubule to the positive end of the microtubule, while dynein moves to the negative end of the microtubule. Kinesin-1 was first identified as a kinesin superfamily protein (KIF) that functions in the intracellular transport of various cargoes, including organelles, neurotransmitter receptors, and mRNA-protein complexes, through interactions between the carboxyl (C)-terminal domain and the cargo. It interacts with other cargoes, but the adapter/scaffold proteins that mediate between kinesin-1 and the cargo have yet to be fully identified. In this study, a yeast two-hybrid screen was used to identify adapter proteins that interact with the C-terminal region of KIF5A. We found an association between the C-terminal region of KIF5A and the cyclin-dependent kinase 2-associated protein 1 (CDK2AP1), originally identified in malignant hamster oral keratinocytes. CDK2AP1 bound to the C-terminal region of KIF5A and did not interact with KIF3A (the motor of kinesin-2), KIF5B, KIF5C, and kinesin light chain 1 (KLC1). The C-terminal region of CDK2AP1 is essential for its interaction with KIF5A. When co-expressed in HEK-293T cells, CDK2AP1 and kinesin-1 co-immunoprecipitated and co-localized in the cells. These results suggest that the KIF5A-CDK2AP1 interaction serves as an adapter protein connecting kinesin-1 and the cargo when kinesin-1 transports cargo in cells.