• Journal of Mushroom
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• v.14 no.3
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• pp.81-89
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• 2016

White button mushroom (Agaricus bisporus) is the most widely consumed mushroom in the world, and domestic consumption is increasing owing to expansion of the westernized diet in recent years. Before 2000, most Korean mushroom farmers cultivated foreign varieties, but recently, Korean breeders are developing hybrid strains using molecular breeding tools. To produce a better mushroom cultivar, we evaluated some traits of button mushroom Agaricus bisporus strains. Mycelial growth rate at different culture temperatures was investigated by 25>20>15>30>$10^{\circ}C$. Recently developed domestic varieties had superior mycelial growth rate compared to the traditionally grown species. In particular, the ASI1338 strain showed excellent mycelial growth rate at different temperatures. Further, we confirmed that ASI1007, ASI1085, ASI1310, ASI1339 were strongly resistant to bacterial brown blotch, whereas ASI1053, ASI1103, ASI1140, ASI1146, ASI1177, ASI1183, ASI1195, ASI1321, ASI1331, ASI1336 were relatively weakly resistant. The average number of days for 16.5, and that for harvest was 5.7; the average yield was 142.7 g/2-kg bag. Mycelial growth rates at 10, 15, 20, $25^{\circ}C$ were similar, but that at $30^{\circ}C$ was lower. The mushroom yield was highly correlated with the number of days for pinheading and harvest.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.941-946
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• 2015

A new Doritaenopsis cultivar 'Hwasu 5205' was bred by Kyungpook National University, Korea, which produces young plants through tissue culture techniques. The new cultivar 'Hwasu 5205', showing the phenotype of vivid red and large flower type characteristics, was derived from crossing between Phalaenopsis Happy Valentine and Doritaenopsis Happy Rose. An elite individual, number '02-05-205' later named as 'Hwasu 5205', was selected among about 300 individual progenies after more than 2 years of intensive selection covering vegetative and flowering distinctiveness. In year 2004-2005, 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'Hwasu 5205' produces vivid red (RHS #PN78B) flowers of i ncurved type with large size, of 9.2 and 12.0 cm in flower height and width, respectively. Leaves of 'Hwasu 5205' grow horizontally and are about 24.3cm in length and 8.5cm in width, respectively. This cultivar possesses no genetic variation. It can be propagated rapidly in vitro and is easy to grow due to its vigorous growth habit. 'Hwasu 5205' was registered (Reg. #: 2915) to Korea Seed & Variety Service (KSVS) on 1st December, 2009 and the PBR(plant breeder's right)is currently controlled by Sangmiwon Orchid Company, Korea.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.651-654
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• 2011

Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.610-616
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• 2011

Clubroot disease caused by Plasmodiophora brassicae, induces damage to cruciferous vegetables worldwide. For control of the disease, many CR (clubroot resistant) $F_1$ hybrid cultivars of Chinese cabbage have been bred and released in Korea. In this study, we determined the race of 10 field isolates of P. brassicae collected from ten regions in Korea using Williams' differential varieties and investigated the degree of resistance of 25 commercial CR cultivars of Chinese cabbage to the isolates. The clubroot pathogens were assigned into two (HS and YC), two (HN1 and HN2), two (DJ and SS) and four (GS, GN, JS, and PC) isolates for race 2, race 4, race 5, race 9, respectively. All CR cultivars showed similar response, resistant or susceptible, to each isolate and the P. brassicae isolates were divided into two groups. Among them, the DJ, GS, GN, HS, and JS isolates could not infect the CR cultivars. In contrast, the SS, HN1, HN2, PC, and YC isolates caused severe clubroot disease on the CR cultivars like susceptible cultivars. Even though they belong to the same race, the CR cultivars showed a different response to the pathogens. The results suggest that the breakdown of CR in Chinese cabbage has already occurred in cultivation areas of Korea and resistance source introduced in CR cultivars may be very limited. In addition, it is likely that resistance genes of Williams' differential varieties to P. brassicae are different from the gene of CR cultivars of Chinese cabbage used in the study.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.49-55
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• 1986

To obtain basic information required for improving grain yield of the two modified opaque-2 synthetics, which have been developed at College of Agr., Chungnam National Univ. in 1980 and named as Puyo No.2 and No.3, physical kernel characteristics of the two synthetics were fully investigated and results obtained are as follows: Puyo No.2 synthetics had a smaller kernel size with lighter weight than the Puyo No.3. The Puyo No.2 synthetics had higher kernel density than the Puyo No.3 with large Kernel size. The Puyo No.2 had kernels with heterogenous endosperm phenotypes. Some kernels had mottled patches on endosperm, while other kernels 1/2 and 1/2 phenotypes. All the modified opaque-2 synthetics had somewhat lighter endosperm weight than the normal check hybrid. The Puyo No.2 synthetics with smaller kernel size had more germ portion compared with large kernel, Puyo No.3. The Puyo No.2 had shown also typical endosperm texture when observed under microscope after cutting by glass knife. The lysine content of the Puyo No.2 was higher than those of other varieties studied. Breeding schemes to improve the yield capacity of the two modified opaue-2 synthetics were discussed.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.224-228
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• 2012

A new $Saxifraga$ $fortunei$ cultivar 'Crystal' was bred by crossing 'Dotaerang' with light pink and light Green (RP 58D) colored petals and 'Rocksun' with light pink and light green white (RP62A + GW157A) colored petals. The selection and flower characterization were conducted from 2004 to 2007 in a greenhouse of the National Institute of Highland Agriculture. The flowering of 'Crystal' occurred from September 21 for 31 days. The number of flowers per plant was 279.0 with many flowering habit. The flower coloration was mixed light pink and green white (RP62D + GW157B) at the opening. The number of petals were 7.0, with a width of 2.1 cm and length of 1.8 cm. The plant height was 15.3 cm and number of leaves was 133.7. 'Crystal' cultivar can be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease would occurs usually by over-irrigation. 'Crystal' cultivar was registered as a new variety with No. 3466 at Korea Seed and Variety Service in April of 2011 year.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.121-126
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• 1995

Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.317-322
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• 1995

As a basic research for breeding new varieties, reciprocal -intergeneric crosses between Citrus junos and P.trifoliata were made. F$_1$ hybrid production using in vitro ovule culture, gametogenesis, and fertilization phenomena were investigated. Frequency of fruit set resulting from crossing of Citrus junos and Poncirus Trifoliata was 16.6% while that of Poncirus Trifoliata and Citrus junos was 11.7%. Callus formation occurred well when ovules at the 6th week after pollination were cultured on MT (Murashige and Tucker) medium supplemented with zeatin 0.5 mg/L and NAA 1.0 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4-D 0.1 or 3.0 mg/L. Immature ovules developed into mature embryos of the MT medium supplemented with 2,4 D 0.1 or 0.5 mg/L. The invitro germination rates of 20-week-old ovules set C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 54.5% and 48.6%, respectively. The emergence ratios of trifoliate hybrids obtained by C. junos $\times$ P. Trifoliata and P. Trifoliata $\times$ C. junos were 56.7% and 100%, respectively. The chromosome number of C. junos and P. Trifoliata was n = 9 or 2n = 18, and the sizes of their pollen grain were 33.75 $\mu$ and 25.0 $\mu$. The length and width of embryo sac in C. junos and P. Trifoliata were 69.38~79.23 $\mu$ and 27.50~38.56 $\mu$, and those of egg cells were 17.50~41.50 $\mu$ and 6.25~8.12$\mu$. Fertilization of C. junos and P. trifoliata terminated 72 h after pollination.

• Journal of Sericultural and Entomological Science
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• v.24 no.1
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• pp.28-31
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• 1982

Flacherie virus (FV) is an important pathogen in the silkworm, which often gives serious damage to farmers for cocoon production. The inbred parents and F$_1$'s from an eight-parent diallel were examined to determine the inheritance of resistance to flacherie virus in the silkworm. Three resistant (R), two intermediate (M) and three susceptible (S) inbreds were used in the diallel with no reciprocals. Mean resistance was measured by survival rates of larvae which were fed on mulberry leaves sprayed with diluted mid-gut homogenate of FV infected larvae. Broad-sense heritability was obtained according to inbreds and F$_1$ family performance. Estimation of general (GCA) and specific combining ability (SCA) was made according to Griffing's Model 1, Method 2. Mean FV resistance of F$_1$ family displayed additive effect of the major gene, while heterotic effect was not significant. Considerable variation in FV resistance within F$_1$ groups of R$\times$S and S$\times$S indicated that action of minor genes for FV resistance may have been involved. FV resistance of inbreds perse and predominant effect of the major gene over minor gene(s) satisfactorily predicted the FV resistance of the hybrids. Broadsense heritability value of FV resistance on the basis of F$_1$ family performance averaged 93%, which suggested that environmental effects might have not been important in this experiment. GCA was highly significant for FV resistant among inbreds. GCA effect of 13.1 in Jam 108 was highest and -17.7 in Gyeongchu lowest. Effective selection for high FV resistance would be possible, using inbreds with high GCA effect and low GCA variance. SCA was significant among hybrids. High SCA effect in the hybrid of Geumho$\times$Mudeung (13.7) and Hansaeng #4$\times$Jam 115 (11.6) indicated that the interaction effect of minor genes for resistance to FV in the silkworm could be exploited by standard silkworm breeding procedures.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.227-231
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• 1984

Chinese CGMS (Cytoplasmic-Genetic Male Sterility) is being transfered to Korean cultivars which will be used as CGMS maintainers. These maintainer lines along with the Chinese CGMS lines and their maintainer lines were crossed with another group of Korean cultivars which will be used as the fertility restorers, and their yield heterosis were examined. The yield heterosis was calculated as high as 113.0-221.1 % in terms of heterosis (F$_1$/Midparent) with the grand mean of 150.5%, and, 86.1-179.8% in terms of heterobeltiosis (F$_1$/better parent) with the grand mean of 125.3%. Among the female parent groups, Chinese mainteiners were the highest following by Chinese MS lines and the Korean maintainers were the lowest. Heterobeltiosis of yield components were 92.7%, 120.0%, 103.0% and 112.0% for number of panicles/unit area, number of grains/panicle, fertility per cent and 1000 grain weight, respectively. Path-coefficients of yield components to the yield were calculated. The highest one was number of grains per panicle ranging 0.8073-0.8649 followed by the 1000 grain weight ranging 0.2000-0.5032. The grain yield ratios to the grand mean were calculated in terms of combining ability. It was highest with the Chinese maintainers followed by Chinese MS lines and The Korean maintainers were lowest. This facts bring cautions to the breeding efforts to transfer the Chinese CGMS in to Korean maintainers.