• Nutritional Sciences
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• v.9 no.1
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• pp.9-13
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• 2006

The differential effects of various fatty acids such as n-3 and n-6 types or degrees of unsaturation on the CYP2E1 induction and the production of lipid peroxidation (LPO) were investigated. The CYP2E1-transduced human hepatoma HepG2 cells (E47) were cultured in RPMI 1640 media containing different concentrations of various fatty acids up to 48 h incubation compared to 04 cells and CYP2E1-null cells. Treated fatty acids were linoleic acid (LA:n-6, C18:2), arachidonic acid (AA:n-6, C20:4) and docosahexaenoic acid (DHA:n-3, C22:6). The cell survival rate was decreased corresponding to the degree of unsaturation (LA>AA $\cong$DHA) and to LPO production in E47 and 04 cells. The four or five unsaturation degree of fatty acids, AA and DHA, caused time- and dose-dependent cell death in E47 cells but not as much as in C34 (without CYP2E1), suggesting an important role of CYP2E1 in the DHA mediated damage. In the levels of lipid peroxides (LPO), AA also elevated LPO by 3- and 5- fold compared to DHA or LA treated E47 cells. However, AA did not increase LPO until 48 h incubation in C34 cells. In conclusion, the polyunsaturated fatty acids induced CYP2E1 induction might be changed by the elevated levels of lipid peroxide (LPO) and oxidative stress through the connection of CYP2E1 and degrees of unsaturated fatty acids.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.793-801
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• 2018

BACKGROUND: The aim of this study was to evaluate the combined effect of low-level laser treatment (LLLT) and recombinant human bone morphological protein-2 (rhBMP-2) applied to hypoxic-cultured MC3T3-E1 osteoblastic cells and to determine possible signaling pathways underlying differentiation and mineralization of osteoblasts under hypoxia. METHODS: MC3T3-E1 cells were cultured under 1% oxygen tension for 72 h. Cell cultures were divided into four groups: normoxia control, low-level laser (LLL) alone, rhBMP-2 combined with LLLT, and rhBMP-2 under hypoxia. Laser irradiation was applied at 0, 24, and 48 h. Cells were treated with rhBMP-2 at 50 ng/mL. Alkaline phosphatase activity was measured at 3, 7, and 14 days to evaluate osteoblastic differentiation. Cell mineralization was determined with Alizarin red S staining at 7 and 14 days. Western blot assays were performed to evaluate whether p38/protein kinase D (PKD) signaling was involved. RESULTS: The results indicate that LLLT and rhBMP-2 synergistically increased alkaline phosphatase (ALP) activity and mineralization. Western blot analyses showed that expression of type I collagen, runt-related transcription factor 2 (RUNX2), and Osterix (Osx), increased and expression of hypoxia-inducible factor 1-alpha ($HIF-1{\alpha}$), decreased more in the LLLT and rhBMP-2 combined group than in the rhBMP-2 or LLL alone groups. Moreover, LLLT and rhBMP-2 stimulated p38 phosphorylation and rhBMP-2 and LLLT increased Prkd1 phosphorylation. CONCLUSION: Combined treatment with rhBMP-2 and LLL induced differentiation and mineralization of hypoxic-cultured MC3T3-E1 osteoblasts by activating p38/PKD signaling in vitro.

• Food Science of Animal Resources
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• v.41 no.6
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• pp.967-982
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• 2021

Probiotics are living microorganisms that, when administered in adequate amounts, provide a health benefit to the host and are considered safe. Most probiotic strains that are beneficial to human health are included in the "Lactic acid bacteria" (LAB) group. The positive effects of probiotic bacteria on the host's health are species-specific and even strain-specific. Therefore, evaluating the probiotic potential of both wild and novel strains is essential. In this study, the probiotic characteristics of Lactobacillus brevis KT38-3 were determined. The strain identification was achieved by 16S rRNA sequencing. API-ZYM test kits were used to determine the enzymatic capacity of the strain. L. brevis KT38-3 was able to survive in conditions with a broad pH range (pH 2-7), range of bile salts (0.3%-1%) and conditions that simulated gastric juice and intestinal juice. The percentage of autoaggregation (59.4%), coaggregation with E. coli O157:H7 (37.4%) and hydrophobicity were determined to be 51.1%, 47.4%, and 52.7%, respectively. L. brevis KT38-3 produced β-galactosidase enzymes and was able ferment lactose. In addition, this strain was capable of producing antimicrobial peptides against the bacteria tested, including methicillin and/or vancomycin-resistant bacteria. The cell-free supernatants of the strain had high antioxidant activities (DPPH: 54.9% and ABTS: 48.7%). Therefore, considering these many essential in vitro probiotic properties, L. brevis KT38-3 has the potential to be used as a probiotic supplement. Supporting these findings with in vivo experiments to evaluate the potential health benefits will be the subject of our future work.

• Mass Spectrometry Letters
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• v.12 no.1
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• pp.1-10
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• 2021

A simple, specific, and economical LC-MS/MS method was investigated for the screening of 43 prescribed antihypertensive and related drugs in human urine. The urine samples were simply prepared by diluting and mixing with internal standard before directly introduced to the LC-MS/MS system, which is fast, straightforward, and cost-effective. Fractional factorial, Box-Behnken, and I-optimal design were applied to screen and optimize the mass spectrometric and chromatographic factors. The analysis was carried out on a triple quadrupole mass spectrometer system utilizing multiple reaction monitoring with positive and negative electrospray ionization method. Chromatographic separation was performed on a Thermo Scientific Accucore RP-MS column (50 × 3.0 mm ID., 2.6 ㎛) using two separate gradient elution programs established with the same mobile phases. Chromatographic separation was performed within 12 min. The optimal method was validated based on FDA guideline. The results indicated that the assay was specific, reproducible, and sensitive with the limit of detection from 0.1 to 50.0 ㎍/L. The method was linear for all analytes with coefficient of determination ranging from 0.9870 to 0.9981. The intra-assay precision was from 1.44 to 19.87% and the inter-assay precision was between 2.69 and 18.54% with the recovery rate ranges from 84.54 to 119.78% for all drugs measured. All analytes in urine samples were stable for 24 h at 25℃, and for 2 weeks at -60℃. The developed method improves on currently existing methods by including larger number of cardiovascular medications and better sensitivity of 12 analytes.

• Journal of the Ergonomics Society of Korea
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• v.10 no.2
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• pp.51-55
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• 1991

Since slipping has been identified as a major hazard, it is important to understand the mechanism of a slip. Many researches, based on biomechanical studies, had been attempted to do that. Thowever, the correlation between the mechanism of a slip and psychophysical behaviors of people had rarely been verified. For example, the existence of small slips in a forward direction, which do not normally perceived by human subjects, has been established by several experimenters. However, the term "microslips are not perceived by the walkers[4]." has not been examined precisely by any experiments. The objective of this study is, using a Signal Detection Theory (SDT), to define a microslip and slip more quqntitatively with the biomechanical measurement of slip distance. The results showed that, the slip distance around the 3 centimeters, there was a obvious change in the accident detectability of the subject. The conclusion is that it is possible to identify the boundary of a microslip and slip around the 3 centermeters of slip distance.

• International Journal of Advanced Culture Technology
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• v.8 no.1
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• pp.19-25
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• 2020

This article describes the automated incubator designs for white mushrooms growing. Mongolia has a very long and cold winter, so the process of growing mushrooms is short. Therefore, we aim to design an automated incubator system that has created a favorable artificial environment for mushroom growing by examining the necessary conditions for mushroom growing. This system was designed to be able to hold for some time the required levels of soil moisture, air humidity, soil temperature, air temperature, and CO2 levels. This article presents the results of a system-based experiment that allows you to grow mushrooms for 30 days without human interference.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.9 no.2
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• pp.7-22
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• 2001

The objective of this study focuses on impacts of stress affecting CRM skills. According to KMOCT Report(1990-1999), aviation accident results in Korea imply that the crew is the primary cause in over 72.7% of accidents. So, CRM continues to receive increasing attention throughout the aviation community to prevent and decrease accidents caused by human factors. In addtion, developing and implementing CRM skills has been a formal part of aviation training. Stress may have a critical impact on the way a pilot assesses decision information, the way they make a decision, and the way they carry that decision through to completion.

• Electronics and Telecommunications Trends
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• v.33 no.2
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• pp.1-9
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• 2018

In the past, the Internet connected people together; recently, however it has been extended to the Internet of Things (IoT), allowing all things in the physical world to be connected. We call a new society in which everything is connected through IoT a 'hyper-connected society'. IoT for a hyper-connected society is more than just connecting things to the Internet, it is an infrastructure providing intelligent services without human intervention by connecting things to the Internet using sensors and communication functions, collecting data from connected things, and analyzing and predicting information. Therefore, IoT is a convergence technology that includes not only sensors and communication networks but also big data and AI. This paper examines the short history of IoT, reviews its current trends, and finally, discusses its future direction.

• Electronics and Telecommunications Trends
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• v.36 no.2
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• pp.1-11
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• 2021

As sensors such as Inertial Measurement Unit, cameras, and Light Detection and Rangings have become cheaper and smaller, research has been actively conducted to implement functions automating micro aerial vehicles such as multirotor type drones. This would fully enable the autonomous flight of drones in the real world without human intervention. In this article, we present a survey of state-of-the-art development on autonomous drones. To build an autonomous drone, the essential components can be classified into pose estimation, environmental perception, and obstacle-free trajectory generation. To describe the trend, we selected three leading research groups-University of Pennsylvania, ETH Zurich, and Carnegie Mellon University-which have demonstrated impressive experiment results on automating drones using their estimation, perception, and trajectory generation techniques. For each group, we summarize the core of their algorithm and describe how they implemented those in such small-sized drones. Finally, we present our up to date research status on developing an autonomous drone.

• BMB Reports
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• v.45 no.12
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• pp.742-747
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• 2012

Granulocyte colony-stimulating factor (G-CSF) is used for heart failure therapy and promotes myocardial regeneration by inducing mobilization of bone marrow stem cells to the injured heart after myocardial infarction; however, this treatment has one weakness in that its biological effect is transient. In our previous report, we generated 5 mutants harboring N-linked glycosylation to improve its antiapoptotic activities. Among them, one mutant (Phe140Asn) had higher cell viability than wild-type hG-CSF in rat cardiomyocytes, even after treatment with an apoptotic agent ($H_2O_2$). Cells treated with this mutant significantly upregulated the antiapoptotic proteins, and experienced reductions in caspase 3 activity and PARP cleavage. Moreover, the total number of apoptotic cells was dramatically lower in cultures treated with mutant hG-CSF. Taken together, these results suggest that the addition of an N-linked glycosylation was successful in improving the antiapoptotic activity of hG-CSF, and that this mutated product will be a feasible therapy for patients who have experienced heart failure.