• Journal of Radiation Protection and Research
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• v.25 no.1
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• pp.3-9
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• 2000

The Korea Atomic Energy Research Institute acquired the Lawrence Livermore National Laboratory phantom for calibration of germanium detectors used for in vivo measurement of radionuclides deposited in human lungs. The manufacturer inscribed concentric circles as a phoswich detector positioning guides on the phantom's torso plate and its overlay plates, and provided the effective thickness of the chest wall for each plate measured at locations over the circles. However, since the germanium detectors are of different sizes, the areas considered for phoswich detectors were no. longer applicable for the locations of the germanium detectors on the phantom. Therefore, we re-evaluated the effective thickness of the phantom to determine if the manufacturer' s data are valid for germanium detectors in use for in vivo lung counting or if new values must be implemented. Differences no more than 3% in effective thickness were found between the germanium detector regions to be used at the Korea Atomic Energy Research Institute and the phoswich detector regions prescribed by the manufacturer.

• Journal of Sasang Constitutional Medicine
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• v.19 no.2
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• pp.11-21
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• 2007

1. Objectives The object of this thesis is to find the basis on why Jejo was assigned as a Taeumin medicine, and to understand Taeumin edema. 2. Methods ${\ulcorner}$Dongyisusebowon${\lrcorner}$ 'Shinchukbon', 'Gabobon', 'Chobonkwun', other related Sasang Constitutional Medicine(SCM) books, herbal medicine books such as ${\ulcorner}$Bonchokangmok${\lrcorner}$, and etc. were compared and researched. 3. Results & Conclusions (1) In Sasang Constitutional Medicine, edema is seen as a critical condition. The first cause of edema in Taeumin is fatigue, a condition where the flow of Qi and Fluid Qi is stagnated. Secondly, edema is caused as Taeeumin is mentally stressed(勞心焦思) and he/she is unable to accomplish his/her plans over many trials or as when they find pleasure in striving for greed. When Lungs which is Incline & Small Viscera(偏小之臟) in Taeumin decreases, it diminishes Real Count of Longevity(命脈實數) which leads to edema formation. (2) Perspective of Human Nature & Character(心性學) and Regimen(養生法) became included gradually in the treatment methods and prescription of edema in Taeumin as ${\ulcorner}$Dongyisusebowon${\lrcorner}$ was revised over time. The revisions also lead to more diversity in prescriptions. (3) Jejo didn't appear In ${\ulcorner}$Dongyisusebowon${\lrcorner}$ 'Chobonkwun' and 'Gabobon'. It was first presented in 'Shinchukbon' as a medicine to treat edema in Taeumin. (4) Jejo have effects of easing clumps. Also it has a strong radiating ability(發散力) which induces perspiration to treat diseases. Finally Jejo is used to remove Takki(濁氣) by utilizing powerful Exhale Gathering Qi contained in it.

• Journal of Haehwa Medicine
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• v.15 no.2
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• pp.39-52
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• 2006

We came to the conclusion as follows from the research about pulse diagnostic technique (脈診) out of "Nan Jinghoeju jeonjung" (難經滙注箋正). 1. "Nan Jing" is practical medical book for clinician and holds different opinions about three portions and nine takings(三部九候脈法) from Huang Di Nei Jing's Taking the pulse of Bu Jung Chim Chon Kwan Chuk((浮中沈 寸關尺) is a quite creative means which has been a important role to future generation. 2. We pointed the differences between western medical science and traditional medical science. And can explain the pulse in western medicine divided from twelve channels of Oriental Medicine. We can explain special connection between heart and lungs using the theory of systemic & pulmonary circulation in western medicine. And this can be a basement of Lung controls every pulse theory in oriental medicine. 3. We have negative assertion about viewing the human body using theory of Yin and Yang-Five Elements. And have poor opinion of explaining about the matching internal organs to Chon Kwan Chuk using the theory of Yin and Yang-Five Elements. 4. We pointed out the mistake that Porak & Sang hwa act for heart. We took pulses considering heart is a real actual internal organ as others. 5. We denied the theory of Man's Chuk pulse is always weak(男子尺脈恒弱) & Woman's Chuk pulse is always strong(女子尺脈恒盛). The physical form of man and woman is not different from each other considering their inheritances from forefather & same shape of organs. So, we cannot insist on the theory.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37 no.6C
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• pp.520-526
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• 2012

Mostly lung diseases by smoking and air pollution is increasing social interest one of 6 kinds of modern diseases which is difficult functional recovery of damaged lung as dangerous diseases of life extension. Therefore, to reduce suffering from respiratory diseases is usually non-smoking, to do strengthen behavior of lung function. In this paper, we would like to propose method to do investigation by voice analysis technology to apply when lung associated ear acupuncture point stimulus to help strengthen actually lung function. From this, we would like to consider the voice change of before/after in smoking to analyze the impact on the human body to the lungs. Based on this experiment, we would like to investigate numerically quantity data actual improved lung function to analyze of voice character difference of before/after in lung associated ear acupuncture point stimulating.

• Korean Journal of Microbiology
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• v.54 no.2
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• pp.152-154
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• 2018

Klebsiella pneumoniae is a Gram-negative, rod-shape bacterium causing disease in human and animal lungs. K. pneumoniae has been often found to gain antimicrobial resistance, thus it has been difficult to treat K. pneumoniae infection with antibiotics. For such infection, bacteriophage can provide an alternative approach for pathogenic bacterial infection with antimicrobial resistance, because of its sensitivity and specificity to the host bacteria. Bacteriophage KP1 was isolated in sewage and showed specific infectivity to K. pneumoniae. Here, we report the draft genome sequence of Klebsiella pneumoniae phage KP1. The draft genome of KP1 is 167,989 bp long, and the G + C content is 39.6%. The genome has 295 predicted ORFs and 14 tRNA genes. In addition, it encodes various enzymes which involve in lysis of the host cell such as lysozyme and holin.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1349-1357
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• 2009

This study was performed to investigate the effects of Shigyungbanha-tang(SGT) on the lipopolysaccharide(LPS) induced acute lung injury(ALI) in mice. 1 and 24 h before LPS intratracheal instillation, control group was taken distilled water orally. Treated groups was taken each concentrate SGT(2.5 g/kg, 6.7 g/kg) by orally as same times. Normal group was not instilled with LPS and was taken distilled water. 24 h after LPS intratracheal instillation, lung histology was performed in inflated-fixed lungs in 3 mice of each groups. The other mice of each groups, bronchoalveolar lavege fluids(BALF) was obtained to measure proinflammatory cytokines(TNF-$\alpha$, IL-$1{\beta}$, IL-6) and blood sample was obtained to measure white blood cell(WBC). In vitro, the effect of SGT($100\;ug/m{\ell}$, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$) on the release of RANTES, TARC induced by TNF-$\alpha$ and IL-4 in human alveolar epithelial cell(A549) was examined. Histopathologically, SGT prevented LPS-induced lung injury. SGT decreased protein, TNF-$\alpha$, IL-$1{\beta}$ and IL-6 according to concentrations. In vitro, $500\;ug/m{\ell}$, $1000\;ug/m{\ell}$ concentrate SGT suppressed the expression of RANTES and TARC on A549 cells. On the basis of these results, SGT had a markedly anti-inflammatory effect in a clinically relevant model of ALI. Nevertheless, further investigations are required to determine the potential clinical usefulness of SGT in the adjunctive therapy of ALI.

• Development and Reproduction
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• v.24 no.3
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• pp.197-205
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• 2020

Diabetes mellitus is a common heterogeneous metabolic disorder, characterized by deposition of extracellular matrix, oxidative stress, and vascular dysfunction, thereby leading to gradual loss of function in multiple organs. However, little attention has been paid to gene expression changes in the lung under hyperglycemic conditions. In this study, we found that diabetes inuced histological changes in the lung of streptozotocin-induced diabetic mice. Global gene expression profiling revealed a set of genes that are up- and down-regulated in the lung of diabetic mice. Among these, expression of Amigo2, Adrb2, and Zbtb16 were confirmed at the transcript level to correlate significantly with hyperglycemia in the lung. We further evaluated the effect of human umbilical cord-derived perivascular stem cells (PVCs) on these gene expression in the lung of diabetic mice. Our results show that administration of PVC-conditioned medium significantly suppressed Amig2, Adrb2, and Zbtb16 upregulation in these mice, suggesting that these genes may be useful indicators of lung injury during hyperglycemia. Furthermore, PVCs offer a promising alternative cell therapy for treating diabetic complications via regulation of gene expression.

• Advances in nano research
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• v.10 no.5
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• pp.461-470
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• 2021

Infection is one of the major mortality causes throughout the globe. Nuclear medicine plays an important role in diagnosis of deep infections such as osteomyelitis, arthritis infection, heart valve and heart prosthesis infections. Techniques such as labeled leukocytes are sensitive and selective for tracking the inflammations but they are not suitable for differentiating infection from inflammation. Anionic linear-globular dendrimer-G₂ was synthesized then conjugation to gemifloxacin antibiotic. The structures were identified by FT-IR, ¹H-NMR, C-NMR, LC-MS and DLS. The toxicity of gemifloxacin and dendrimer-gemifloxacin complex was compared by MTT test. Dendrimer-G₂-gemifloxacin was labeled by Technetium-99m and its in-vitro stability and radiochemical purity were investigated. In-vivo biodistribution and SPECT imaging were studied in a rabbit model. Identify and verify the structure of the each object was confirmed by FT-IR, ¹H-NMR, C-NMR and LC-MS, also, the size and charge of this compound were 128 nm and -3/68 mv respectively. MTT test showed less toxicity of the dendrimer-G₂-gemifloxacin than free gemifluxacin (P < 0.001). Radiochemical yield was > %98. Human serum stability was 84% up to 24 h. Biodistribution study at 50 min, 24 and 48 h showed that the complex is significantly absorbed by the intestine and accumulation in the lungs and affects them, finally excreted through the kidneys, biodistribution results are consistent with results from full image means of SPECT/CT technique.

• Tuberculosis and Respiratory Diseases
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• v.84 no.3
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• pp.200-208
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• 2021

Background: Hypersensitivity pneumonitis (HP) is an increasingly recognized form of diffuse parenchymal lung disease. Krebs von den Lungen-6 (KL-6) is now classified as a human MUC1 mucin protein, and regenerating type II pneumocytes are the primary cellular source of KL-6/MUC1 in the affected lungs of patients with interstitial lung diseases (ILD). Serum KL-6/MUC1 levels have been demonstrated to be useful for the evaluation of various ILD. To determine the role of circulating KL-6 in evaluating the disease activity and management of HP. Methods: An observational cross-sectional study was conducted on 51 patients with HP and 20 healthy controls. Serum KL-6 levels were measured in both groups. Patients were further assessed based on chest high-resolution computed tomography (HRCT), pulmonary function test, 6-minute walk test, echocardiography, bronchioalveolar lavage, and/or transbronchial biopsy. Patients were divided into the fibrotic and non-fibrotic groups according to the HRCT findings. Results: The median serum KL-6 levels were significantly higher in HP patients as compared to the control group. The median serum KL-6 levels were found to be higher in the non-fibrotic HP group (1,900 IU/mL) as compared to the fibrotic group (1,200 IU/mL). There was a significant inverse correlation between serum KL-6 serum level and the dose of steroids as well as the duration of steroid therapy. Conclusion: The presence of higher KL-6 levels in the non-fibrotic HP group implies its enhanced production by regenerating pneumocytes in response to alveolar injury. The significant association between serum KL-6 levels and the dose and the duration of steroid therapy emphasizes the significant role of steroids in the stabilization of the disease.

• Molecules and Cells
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• v.45 no.11
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• pp.833-845
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• 2022

Although asthma is a common chronic airway disease that responds well to anti-inflammatory agents, some patients with asthma are unresponsive to conventional treatment. Mesenchymal stem cells (MSCs) have therapeutic potential for the treatment of inflammatory diseases owing to their immunomodulatory properties. However, the target cells of MSCs are not yet clearly known. This study aimed to determine the effect of human umbilical cord-derived MSCs (hUC-MSCs) on asthmatic lungs by modulating innate immune cells and effector T cells using a murine asthmatic model. Intravenously administered hUC-MSCs reduced airway resistance, mucus production, and inflammation in the murine asthma model. hUC-MSCs attenuated not only T helper (Th) 2 cells and Th17 cells but also augmented regulatory T cells (Tregs). As for innate lymphoid cells (ILC), hUC-MSCs effectively suppressed ILC2s by downregulating master regulators of ILC2s, such as Gata3 and Tcf7. Finally, regarding lung macrophages, hUC-MSCs reduced the total number of macrophages, particularly the proportion of the enhanced monocyte-derived macrophage population. In a closer examination of monocyte-derived macrophages, hUC-MSCs reduced the M2a and M2c populations. In conclusion, hUC-MSCs can be considered as a potential anti-asthmatic treatment given their therapeutic effect on the asthmatic airway inflammation in a murine asthma model by modulating innate immune cells, such as ILC2s, M2a, and M2c macrophages, as well as affecting Tregs and effector T cells.